Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,717,954	G→T	6.1%	E173D (GAG→GAT)	fabB →	3‑oxoacyl‑ACP synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,717,954	0	G	T	6.1%	287.5 / 5.2	91	E173D (GAG→GAT)	fabB	3‑oxoacyl‑ACP synthase
Reads supporting (aligned to +/- strand):  ref base G (36/48);  new base T (4/2);  total (40/51)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

AAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGCTG  >  NC_002947/4717808‑4718102                                                                                                                                                   |                                                                                                                                                     aaGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAg                                                                                                                                                      >  3:336835/1‑147 (MQ=255) aaGCGCGTAGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCa                                                                                                                                                    >  1:286846/1‑149 (MQ=255)   gcgcGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGa                                                                                                                                                  <  5:102578/149‑1 (MQ=255)   gcgcGTCGG‑CCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAg                                                                                                                                                      >  3:83878/1‑144 (MQ=255)          ggCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCTATCTCGTCGGCCTGCGCCACCTCTGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGt                                                                                                                                            <  2:286846/148‑1 (MQ=255)          ggCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTg                                                                                                                                           <  2:296216/149‑1 (MQ=255)          ggCCCATACCGCGTGACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTg                                                                                                                                           >  7:370635/1‑149 (MQ=255)           gCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTgg                                                                                                                                          >  1:249814/1‑149 (MQ=255)           gCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTgg                                                                                                                                          <  8:370635/149‑1 (MQ=255)            cccATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCAGCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTggg                                                                                                                                         <  1:357466/149‑1 (MQ=255)             ccATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTgggg                                                                                                                                        >  5:117693/1‑149 (MQ=255)             ccATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTgggg                                                                                                                                        >  1:436660/1‑149 (MQ=255)               aTACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGc                                                                                                                                       >  7:269142/1‑148 (MQ=255)               aTACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGc                                                                                                                                       >  1:128891/1‑148 (MQ=255)               aTACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACATCGGCTCACTGCAGCGGTACCCCACTGGAGCAGATCCAGTGGGGc                                                                                                                                       >  2:431607/1‑148 (MQ=255)                 aCCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTggg                                                                                                                                         >  1:194288/1‑144 (MQ=255)                 aCCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTggg                                                                                                                                         <  2:194288/144‑1 (MQ=255)                  ccGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAg                                                                                                                                    <  2:225944/148‑1 (MQ=255)                    gcgTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAg                                                                                                                                 <  4:271565/149‑1 (MQ=255)                      gTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGa                                                                                                                               <  4:83878/149‑1 (MQ=255)                          cccGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATc                                                                                                                           <  6:72187/149‑1 (MQ=255)                           ccGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCGACCGCGTTCATGATGTAGGGCAGCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCGGACTGCAGCGGTACCGCCCTGGAGGAGATCCCGTGGGGCAAGCAGGCCATCg                                                                                                                          >  4:169986/1‑149 (MQ=255)                            cGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGATCTGCTCCAGTgcgg                                                                                                                                        <  7:292421/134‑1 (MQ=255)                            cGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGATCTGCTCCAGTgcgg                                                                                                                                        >  8:292421/1‑134 (MQ=255)                            cGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGt                                                                                                                         <  7:104479/149‑1 (MQ=255)                             gCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTc                                                                                                                        >  5:175405/1‑149 (MQ=255)                              cACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATTGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCt                                                                                                                       >  2:86972/1‑149 (MQ=255)                                 cATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCg                                                                                                                    >  3:136400/1‑149 (MQ=255)                                     ggCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCg                                                                                                                 <  3:422108/148‑1 (MQ=255)                                       cagcaCCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTg                                                                                                              <  2:436660/149‑1 (MQ=255)                                          gacggtgtCGGCATGCCGGGCCACCCCGTTCAAGATCAACGGCATCATCTTCTCGATCTCGTCGGCGTGCGCCACCTCGGCTCACTGCATCGGTACCGCCCTGCAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCTGTGGCg                                                                                                           <  8:412129/145‑1 (MQ=255)                                          caCCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTAGTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTAGCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCg                                                                                                           <  5:318315/149‑1 (MQ=255)                                          caCCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCg                                                                                                           <  6:375400/149‑1 (MQ=255)                                          caCCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCg                                                                                                           <  8:108872/149‑1 (MQ=255)                                            ccGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGt                                                                                                         <  5:254657/149‑1 (MQ=255)                                             cGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTg                                                                                                        <  3:136969/149‑1 (MQ=255)                                             cGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTg                                                                                                        <  2:249814/149‑1 (MQ=255)                                               tgtCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGTCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTgaa                                                                                                      <  6:111341/149‑1 (MQ=255)                                                   ggCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGaagaag                                                                                                  <  1:174246/149‑1 (MQ=255)                                                   ggCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGaagaag                                                                                                  <  1:339425/149‑1 (MQ=255)                                                    gCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAagaaga                                                                                                 <  5:125409/149‑1 (MQ=255)                                                     cATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAagag                                                                                                <  6:175405/149‑1 (MQ=255)                                                      aTGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGc                                                                                               >  8:334429/1‑149 (MQ=255)                                                         ccTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGGGGCGGTGAAGAAGAGCAc                                                                                             <  8:237479/148‑1 (MQ=255)                                                           tcgCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGACTGCGCCACCTCGCCTCACTCCATCGGTACCGCAATGGAGAACAACAACAGGGGCAACCAGGACATCGTCTTCCCCCGAGGCCCTGAAGAAcaccacag                                                                                           >  8:94063/3‑146 (MQ=255)                                                             gCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAg                                                                                        >  2:87008/1‑149 (MQ=255)                                                              ccACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCTCTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGc                                                                                       <  6:285047/149‑1 (MQ=255)                                                              ccACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGc                                                                                       <  4:336835/149‑1 (MQ=255)                                                                      ttCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGTACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGtt                                                                               <  3:169986/149‑1 (MQ=255)                                                                       tCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTc                                                                              >  3:408854/1‑149 (MQ=255)                                                                        cAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTc                                                                              <  2:128891/148‑1 (MQ=255)                                                                        cAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTc                                                                              <  1:87008/148‑1 (MQ=255)                                                                         aaGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCAc                                                                                             <  4:383896/132‑1 (MQ=255)                                                                         aaGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCAc                                                                                             >  3:383896/1‑132 (MQ=255)                                                                         aaGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCt                                                                            <  8:292589/149‑1 (MQ=255)                                                                         aaGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCt                                                                            >  5:276257/1‑149 (MQ=255)                                                                         aaGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTAACGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCt                                                                            >  3:287148/1‑149 (MQ=255)                                                                           gATCAAGGGCATCAACTACTCGATCTTGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGCCATCGTCTTAGCCGGTGGCGGTGAAGAAGAGCACTGTAGCCAGACGTTCCTGt                                                                          >  6:285611/1‑149 (MQ=255)                                                                           gATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGt                                                                          >  5:26442/1‑149 (MQ=255)                                                                               aaGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCgt                                                                      >  7:409404/1‑148 (MQ=255)                                                                               aaGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGa                                                                      <  1:431607/149‑1 (MQ=255)                                                                               aaGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACAGCACTGGAGCAGATCCAGTGGGGAAAGCAGGACAACGTCTTCGCCGGTGGCGGTGAAGAAGAGCACAGCAGCCAGTCGTTACTGttaca                                                                      >  7:142661/1‑146 (MQ=255)                                                                                     aTCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCAt                                                                >  5:245331/1‑149 (MQ=255)                                                                                      tCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATg                                                               >  1:288734/1‑149 (MQ=255)                                                                                      tCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATg                                                               >  7:96/1‑149 (MQ=255)                                                                                        aaCTACTCGATCTTGTCGGCCTGCGACAACTCGGCTCACTTCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATgg                                                              <  5:285611/148‑1 (MQ=255)                                                                                         actactCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGATCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGgc                                                            <  7:94063/149‑1 (MQ=255)                                                                                         actactCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGgc                                                            >  6:194856/1‑149 (MQ=255)                                                                                             ctCGATCTCGTCGGCCTGCGCTACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGccc                                                        >  1:255533/1‑149 (MQ=255)                                                                                             ctCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATTGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAGGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGgcgc                                                          <  1:86972/147‑1 (MQ=255)                                                                                             ctCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGcc                                                         <  7:271760/148‑1 (MQ=255)                                                                                              tCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCt                                                       >  7:237623/1‑149 (MQ=255)                                                                                               cGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTg                                                      >  7:280443/1‑149 (MQ=255)                                                                                                gATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCt                                                       <  1:43872/147‑1 (MQ=255)                                                                                                  tctcGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCg                                                   >  5:319021/1‑149 (MQ=255)                                                                                                   ctcGTCGGCCTGCCCCACCTAGGCTACCTCCCACGCTACCGCAGTCGACCACCCCCAGGGGGGCAAGCAGGACATCGACTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGa                                                  <  8:379597/149‑1 (MQ=255)                                                                                                      gtcgGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTc                                                    <  6:245331/144‑1 (MQ=255)                                                                                                      gtcgGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCg                                                   <  4:136400/145‑1 (MQ=255)                                                                                                           ccTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAgcgc                                          <  7:110247/149‑1 (MQ=255)                                                                                                               cgcCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCaa                                        <  2:288734/147‑1 (MQ=255)                                                                                                                  cACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGaaa                                   >  3:109395/1‑149 (MQ=255)                                                                                                                   aCCTCGGCTCACTGCATCGGTACTGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAAc                                  >  6:13954/1‑149 (MQ=255)                                                                                                                        ggCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCg                              <  7:334429/148‑1 (MQ=255)                                                                                                                             aCTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACt                         <  5:194856/148‑1 (MQ=255)                                                                                                                               tGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGc                      >  3:413220/1‑149 (MQ=255)                                                                                                                                 cATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGc                      <  8:237623/147‑1 (MQ=255)                                                                                                                                 cATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGGCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGc                      <  6:117693/147‑1 (MQ=255)                                                                                                                                  aTCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTc                   <  7:159505/149‑1 (MQ=255)                                                                                                                                      gTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAACCCCCGGAACTGGCCTcgcgc               >  8:140383/1‑149 (MQ=255)                                                                                                                                         ccccACTGGATCTGCTCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCgcgcgc             >  8:260630/4‑148 (MQ=255)                                                                                                                                           ccACTGGATCTGCTCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAAGCCCGGAACTGGCCTCGCGCGCCTa          >  6:379890/2‑149 (MQ=255)                                                                                                                                           ccACTGGATCTGCTCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTa          >  7:62931/2‑149 (MQ=255)                                                                                                                                                 gAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTacgacg     <  8:280443/148‑1 (MQ=255)                                                                                                                                                   gCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGCTg  <  6:276257/149‑1 (MQ=255)                                                                                                                                                   gCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGAAAAGAAACCCCGCAACTGGCCTCGCGCGCCTACTACGCt   >  8:383952/1‑148 (MQ=255)                                                                                                                                                   |                                                                                                                                                     AAGCGCGTCGGCCCATACCGCGTTACCCGCACCATGGGCAGCACCGTGTCGGCATGCCTGGCCACCCCGTTCAAGATCAAGGGCATCAACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGCTG  >  NC_002947/4717808‑4718102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑