Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,740,330	A→C	7.1%	D286A (GAC→GCC)	gltA →	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,740,330	0	A	C	7.1%	208.6 / 6.4	70	D286A (GAC→GCC)	gltA	citrate synthase
Reads supporting (aligned to +/- strand):  ref base A (32/33);  new base C (2/3);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

CACCGTTCGTCTGGCAGGCTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCTGCGCGAGCTGGGCATCAAGAACGAC  >  NC_002947/4740184‑4740478                                                                                                                                                     |                                                                                                                                                     caccGTTCGTCTGGCAGGCTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGAc                                                                                                                                                     >  5:355087/1‑148 (MQ=255) caccGTTCGTCTGGCAGGCTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACa                                                                                                                                                    <  3:54510/149‑1 (MQ=255)         gTCTGGCAGGCTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ATATCGACAAATTCATc                                                                                                                                            <  4:282674/149‑1 (MQ=255)          gcttgCAGGCTCGTCGGGTGCCACCCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTTGGGCCGGT‑GCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATCAAATCGGCTATGTCTCGA‑ACATCGACAAATTCATCg                                                                                                                                           <  8:296866/145‑1 (MQ=255)             ggCAGGCTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCgcc                                                                                                                                         >  6:247082/1‑148 (MQ=255)              gCAGGCTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAATTTGTCGATGT‑TCGAGACATCGCCGATTTCATcca                                                                                                                                          <  4:348323/146‑3 (MQ=37)                 ggCTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCgccaaggc                                                                                                                                    <  2:133240/149‑1 (MQ=255)                   cTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAATTTGTCGATGT‑TCGAGACATCGCCGATTTCATcca                                                                                                                                          <  8:345695/141‑3 (MQ=37)                   cTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAATTTGTCGATGT‑TCGAGACATCGCCGATTTCATcca                                                                                                                                          >  7:345695/1‑139 (MQ=37)                    tcgtcgGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAATTTGTCGATGT‑TCGAGACATCGCCGATTTCATccagcatg                                                                                                                                     <  2:148152/145‑8 (MQ=25)                    tcgtcgGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAATTTGTCGATGT‑TCGAGACATCGCCGATTTCATccagcatg                                                                                                                                     >  1:148152/1‑138 (MQ=25)                    tcgtcgGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAAt                                                                                                                                                 <  3:418845/133‑1 (MQ=255)                    tcgtcgGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAAt                                                                                                                                                 >  4:418845/1‑133 (MQ=255)                    tcgtcgGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAAGTCATCgccaaggcc                                                                                                                                   >  8:295858/1‑147 (MQ=255)                      gtcgTGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCgccaaggccaag                                                                                                                                >  3:231877/1‑148 (MQ=255)                       tcgGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGa                                                                                                                              >  2:1205/1‑149 (MQ=255)                              ccAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATc                                                                                                                                            >  7:282576/1‑128 (MQ=255)                              ccAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATc                                                                                                                                            <  8:282576/128‑1 (MQ=255)                                 aCCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGAc                                                                                                                    <  2:265743/149‑1 (MQ=255)                                         gCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTc                                                                                                              >  1:229661/1‑147 (MQ=255)                                         gCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCaa                                                                                                            <  4:390568/149‑1 (MQ=255)                                             gTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACaaa                                                                                                                                                  >  5:132170/1‑107 (MQ=255)                                             gTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACaaa                                                                                                                                                  <  6:132170/107‑1 (MQ=255)                                             gTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCgc                                                                                                                                          <  6:427988/115‑1 (MQ=255)                                             gTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCgc                                                                                                                                          >  5:427988/1‑115 (MQ=255)                                              tATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGa                                                                                                       <  7:391829/149‑1 (MQ=255)                                              tATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGa                                                                                                       <  7:371307/149‑1 (MQ=255)                                                tCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCgccaaggccaa                                                                                                                                 <  8:6564/121‑1 (MQ=255)                                                tCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCgccaaggccaa                                                                                                                                 >  7:6564/1‑121 (MQ=255)                                                tCGCAGACGTTATCGAAGAACTGTGGGTCCAGG‑CCAAATTCAGTGCGAACGAAGACGTACTGACCATGCTGGATGTAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGACAAGGCAAAGGACAAGAACGACCCCTTCTAGCTGAtc                                                                                                     >  4:70823/1‑148 (MQ=255)                                                 cgccgcCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGACAAGGCCAAGGACAAGAACGACCCGTTCAAGCGGATgg                                                                                                    >  8:290221/1‑149 (MQ=255)                                                    cgccgGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCCAGCTGATGGGCt                                                                                                 >  6:202496/1‑149 (MQ=255)                                                     gccgGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCtt                                                                                                <  1:98589/149‑1 (MQ=255)                                                       cgGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCg                                                                                              <  2:290653/149‑1 (MQ=255)                                                         gTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGt                                                                                            >  1:317769/1‑149 (MQ=255)                                                          tATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGt                                                                                            >  1:420081/1‑148 (MQ=255)                                                            tCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCa                                                                                          <  2:342488/148‑1 (MQ=255)                                                               ccgcACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCGAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACcgc                                                                                      >  7:141271/1‑149 (MQ=255)                                                                 gcACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCgt                                                                                    <  2:229661/149‑1 (MQ=255)                                                                    gccgtgggccgggccCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCgtg                                                                                   >  6:431244/6‑148 (MQ=255)                                                                        ggggCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTGAAGCTGATGGGCTTCGGTCACCGCGTGTAc                                                                                >  1:116012/1‑146 (MQ=255)                                                                        ggggCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAg                                                                             <  5:15440/149‑1 (MQ=255)                                                                          ggcccgg‑cccACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCCAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGaa                                                                           >  7:102704/1‑149 (MQ=255)                                                                           gcccgg‑cccACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAAc                                                                          >  6:431162/1‑149 (MQ=255)                                                                                    cACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGAccc                                                                  >  8:197359/1‑149 (MQ=255)                                                                                              cgaacgaaGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCAcc                                                        >  6:36195/1‑149 (MQ=255)                                                                                                  cgaaGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGtga                                                    <  1:71911/149‑1 (MQ=255)                                                                                                   gaaGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGtgat                                                   >  6:376579/1‑149 (MQ=255)                                                                                                    aaGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGtgatg                                                  <  5:247082/149‑1 (MQ=255)                                                                                                       ccGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAg                                               <  7:197359/149‑1 (MQ=255)                                                                                                           aCTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGa                                           >  8:364174/1‑149 (MQ=255)                                                                                                            cTGACCATGCTCGTTGAAATCCGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTTTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGAc                                          <  4:238617/149‑1 (MQ=255)                                                                                                               aCCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCt                                        <  5:431162/148‑1 (MQ=255)                                                                                                                ccATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGc                                      <  5:431244/149‑1 (MQ=255)                                                                                                                 cATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGcg                                     >  3:33868/1‑149 (MQ=255)                                                                                                                  aTGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGcga                                    <  7:364174/149‑1 (MQ=255)                                                                                                                     cTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGcgacg                                  <  2:317769/148‑1 (MQ=255)                                                                                                                      tGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGaa                                <  2:116012/149‑1 (MQ=255)                                                                                                                        gATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCCACGaa                                <  7:392771/147‑1 (MQ=255)                                                                                                                           gAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCt                           >  3:213457/1‑149 (MQ=255)                                                                                                                            aaaTCGGCGTTGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCTg                          >  6:411107/1‑149 (MQ=255)                                                                                                                                   cGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCTGCGCGAGc                   <  2:420081/149‑1 (MQ=255)                                                                                                                                   cGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCTGCGCGAGc                   >  8:219437/1‑149 (MQ=255)                                                                                                                                         ctcGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGCGCTTCGGTCACCGCGTGTCCAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTCCGACGAAGTCCTGCGCGAGCTGGGc              >  7:280407/1‑148 (MQ=255)                                                                                                                                            gA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCTGCGCGAGCTGGGCa             <  1:1205/146‑1 (MQ=255)                                                                                                                                            gA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCTGCGCGAGCTGGGCATc           <  5:376579/148‑1 (MQ=255)                                                                                                                                             a‑aCATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCTGCGCGAGCTGGGCATCaa         <  4:33868/149‑1 (MQ=255)                                                                                                                                                cATCGACAAATTCATCGCCAAGGGCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCCCCGTGATGAAGCAGACCTGCGACGAAGTCCTGCGCGAGCTGGGCATCAAg        >  4:60781/1‑148 (MQ=255)                                                                                                                                                cATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCTGCGCGAGCTGGGCATCAAg        >  8:164736/1‑148 (MQ=255)                                                                                                                                                 aTCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCTGCGCGAGCTGGGCATCAAGaa      <  3:70823/149‑1 (MQ=255)                                                                                                                                                     aCAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCTGCGCGAGCTGGGCATCAAGAACGAc  <  4:231877/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     CACCGTTCGTCTGGCAGGCTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCTGCGCGAGCTGGGCATCAAGAACGAC  >  NC_002947/4740184‑4740478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑