Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,740,334	A→T	7.3%	K287N (AAA→AAT) ‡	gltA →	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,740,334	0	A	T	7.3%	197.0 / 4.8	68	K287N (AAA→AAT) ‡	gltA	citrate synthase
Reads supporting (aligned to +/- strand):  ref base A (30/33);  new base T (2/3);  total (32/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

GTCTGGCAGGCTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCTGCGCGAGCTGGGCATCAAGAACGACC  >  NC_002947/4740192‑4740479                                                                                                                                                 |                                                                                                                                                  gTCTGGCAGGCTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ATATCGACAAATTCATc                                                                                                                                             <  4:282674/149‑1 (MQ=255)  gcttgCAGGCTCGTCGGGTGCCACCCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTTGGGCCGGT‑GCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATCAAATCGGCTATGTCTCGA‑ACATCGACAAATTCATCg                                                                                                                                            <  8:296866/145‑1 (MQ=255)     ggCAGGCTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCgcc                                                                                                                                          >  6:247082/1‑148 (MQ=255)      gCAGGCTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAATTTGTCGATGT‑TCGAGACATCGCCGATTTCATcca                                                                                                                                           <  4:348323/146‑3 (MQ=37)         ggCTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCgccaaggc                                                                                                                                     <  2:133240/149‑1 (MQ=255)           cTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAATTTGTCGATGT‑TCGAGACATCGCCGATTTCATcca                                                                                                                                           <  8:345695/141‑3 (MQ=37)           cTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAATTTGTCGATGT‑TCGAGACATCGCCGATTTCATcca                                                                                                                                           >  7:345695/1‑139 (MQ=37)            tcgtcgGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAATTTGTCGATGT‑TCGAGACATCGCCGATTTCATccagcatg                                                                                                                                      >  1:148152/1‑138 (MQ=25)            tcgtcgGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAATTTGTCGATGT‑TCGAGACATCGCCGATTTCATccagcatg                                                                                                                                      <  2:148152/145‑8 (MQ=25)            tcgtcgGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAAt                                                                                                                                                  >  4:418845/1‑133 (MQ=255)            tcgtcgGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAAt                                                                                                                                                  <  3:418845/133‑1 (MQ=255)            tcgtcgGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAAGTCATCgccaaggcc                                                                                                                                    >  8:295858/1‑147 (MQ=255)              gtcgTGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCgccaaggccaag                                                                                                                                 >  3:231877/1‑148 (MQ=255)               tcgGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGa                                                                                                                               >  2:1205/1‑149 (MQ=255)                      ccAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATc                                                                                                                                             <  8:282576/128‑1 (MQ=255)                      ccAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATc                                                                                                                                             >  7:282576/1‑128 (MQ=255)                         aCCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGAc                                                                                                                     <  2:265743/149‑1 (MQ=255)                                 gCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTc                                                                                                               >  1:229661/1‑147 (MQ=255)                                 gCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCaa                                                                                                             <  4:390568/149‑1 (MQ=255)                                     gTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACaaa                                                                                                                                                   <  6:132170/107‑1 (MQ=255)                                     gTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACaaa                                                                                                                                                   >  5:132170/1‑107 (MQ=255)                                     gTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCgc                                                                                                                                           >  5:427988/1‑115 (MQ=255)                                     gTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCgc                                                                                                                                           <  6:427988/115‑1 (MQ=255)                                      tATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGa                                                                                                        <  7:371307/149‑1 (MQ=255)                                      tATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGa                                                                                                        <  7:391829/149‑1 (MQ=255)                                        tCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCgccaaggccaa                                                                                                                                  >  7:6564/1‑121 (MQ=255)                                        tCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCgccaaggccaa                                                                                                                                  <  8:6564/121‑1 (MQ=255)                                        tCGCAGACGTTATCGAAGAACTGTGGGTCCAGG‑CCAAATTCAGTGCGAACGAAGACGTACTGACCATGCTGGATGTAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGACAAGGCAAAGGACAAGAACGACCCCTTCTAGCTGAtc                                                                                                      >  4:70823/1‑148 (MQ=255)                                         cgccgcCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGACAAGGCCAAGGACAAGAACGACCCGTTCAAGCGGATgg                                                                                                     >  8:290221/1‑149 (MQ=255)                                            cgccgGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCCAGCTGATGGGCt                                                                                                  >  6:202496/1‑149 (MQ=255)                                             gccgGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCtt                                                                                                 <  1:98589/149‑1 (MQ=255)                                               cgGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCg                                                                                               <  2:290653/149‑1 (MQ=255)                                                 gTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGt                                                                                             >  1:317769/1‑149 (MQ=255)                                                  tATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGt                                                                                             >  1:420081/1‑148 (MQ=255)                                                    tCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCa                                                                                           <  2:342488/148‑1 (MQ=255)                                                       ccgcACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCGAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACcgc                                                                                       >  7:141271/1‑149 (MQ=255)                                                         gcACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCgt                                                                                     <  2:229661/149‑1 (MQ=255)                                                            gccgtgggccgggccCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCgtg                                                                                    >  6:431244/6‑148 (MQ=255)                                                                ggggCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTGAAGCTGATGGGCTTCGGTCACCGCGTGTAc                                                                                 >  1:116012/1‑146 (MQ=255)                                                                ggggCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAg                                                                              <  5:15440/149‑1 (MQ=255)                                                                  ggcccgg‑cccACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCCAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGaa                                                                            >  7:102704/1‑149 (MQ=255)                                                                   gcccgg‑cccACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAAc                                                                           >  6:431162/1‑149 (MQ=255)                                                                            cACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGAccc                                                                   >  8:197359/1‑149 (MQ=255)                                                                                      cgaacgaaGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCAcc                                                         >  6:36195/1‑149 (MQ=255)                                                                                          cgaaGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGtga                                                     <  1:71911/149‑1 (MQ=255)                                                                                           gaaGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGtgat                                                    >  6:376579/1‑149 (MQ=255)                                                                                            aaGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGtgatg                                                   <  5:247082/149‑1 (MQ=255)                                                                                               ccGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAg                                                <  7:197359/149‑1 (MQ=255)                                                                                                   aCTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGa                                            >  8:364174/1‑149 (MQ=255)                                                                                                    cTGACCATGCTCGTTGAAATCCGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTTTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGAc                                           <  4:238617/149‑1 (MQ=255)                                                                                                       aCCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCt                                         <  5:431162/148‑1 (MQ=255)                                                                                                        ccATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGc                                       <  5:431244/149‑1 (MQ=255)                                                                                                         cATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGcg                                      >  3:33868/1‑149 (MQ=255)                                                                                                          aTGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGcga                                     <  7:364174/149‑1 (MQ=255)                                                                                                             cTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGcgacg                                   <  2:317769/148‑1 (MQ=255)                                                                                                              tGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGaa                                 <  2:116012/149‑1 (MQ=255)                                                                                                                gATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCCACGaa                                 <  7:392771/147‑1 (MQ=255)                                                                                                                   gAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCt                            >  3:213457/1‑149 (MQ=255)                                                                                                                    aaaTCGGCGTTGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCTg                           >  6:411107/1‑149 (MQ=255)                                                                                                                           cGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCTGCGCGAGc                    <  2:420081/149‑1 (MQ=255)                                                                                                                           cGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCTGCGCGAGc                    >  8:219437/1‑149 (MQ=255)                                                                                                                                 ctcGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGCGCTTCGGTCACCGCGTGTCCAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTCCGACGAAGTCCTGCGCGAGCTGGGc               >  7:280407/1‑148 (MQ=255)                                                                                                                                    gA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCTGCGCGAGCTGGGCa              <  1:1205/146‑1 (MQ=255)                                                                                                                                    gA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCTGCGCGAGCTGGGCATc            <  5:376579/148‑1 (MQ=255)                                                                                                                                     a‑aCATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCTGCGCGAGCTGGGCATCaa          <  4:33868/149‑1 (MQ=255)                                                                                                                                        cATCGACAAATTCATCGCCAAGGGCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCCCCGTGATGAAGCAGACCTGCGACGAAGTCCTGCGCGAGCTGGGCATCAAg         >  4:60781/1‑148 (MQ=255)                                                                                                                                        cATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCTGCGCGAGCTGGGCATCAAg         >  8:164736/1‑148 (MQ=255)                                                                                                                                         aTCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCTGCGCGAGCTGGGCATCAAGaa       <  3:70823/149‑1 (MQ=255)                                                                                                                                             aCAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCTGCGCGAGCTGGGCATCAAGAACGAc   <  4:231877/149‑1 (MQ=255)                                                                                                                                              cAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCTGCGCGAGCTGGGCATCAAGAACGAcc  <  5:411107/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                  GTCTGGCAGGCTCGTCGGGTGCCAACCCGTTCGCCTGTATCGCCGCCGGTATCGCCGCACTGTGGGGCCCGG‑CCCACGGCGGTGCGAACGAAGCCGTACTGACCATGCTGGATGAAATCGGCGATGTCTCGA‑ACATCGACAAATTCATCGCCAAGGCCAAGGACAAGAACGACCCGTTCAAGCTGATGGGCTTCGGTCACCGCGTGTACAAGAACCGCGACCCGCGCGCCACCGTGATGAAGCAGACCTGCGACGAAGTCCTGCGCGAGCTGGGCATCAAGAACGACC  >  NC_002947/4740192‑4740479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑