| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,796,617 | G→C | 30.0% | intergenic (‑175/+43) | pvdH ← / ← PP_4224 | diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,796,617 | 0 | G | C | 30.0% | 38.9 / 22.0 | 30 | intergenic (‑175/+43) | pvdH/PP_4224 | diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase |
| Reads supporting (aligned to +/- strand): ref base G (11/10); new base C (6/3); total (17/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.47e-01 | |||||||||||
CGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATC > NC_002947/4796475‑4796765 | cGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCgg < 8:172732/149‑1 (MQ=255) cATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTAcc < 1:407288/149‑1 (MQ=38) aTGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTAccc < 7:298917/149‑1 (MQ=38) gTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCcgcg > 7:105740/1‑148 (MQ=255) tAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGaa < 6:51017/149‑1 (MQ=255) aaTCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAaga < 1:217281/149‑1 (MQ=255) aTCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCCCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAagag < 8:294929/149‑1 (MQ=37) tCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTTGAAACGCTTGTGTGGGGGTAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCTGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAgg < 4:265081/149‑1 (MQ=255) ggCGGTTACCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACAt < 5:213224/149‑1 (MQ=255) aaaCGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGc > 5:346870/1‑113 (MQ=255) aaaCGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGc < 6:346870/113‑1 (MQ=255) aGCGGTTGTTACGGGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCaa < 8:105740/148‑1 (MQ=255) tCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAg < 7:297853/148‑1 (MQ=255) ttCGCGGGTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCg > 4:30752/1‑149 (MQ=37) cgcgGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGa > 4:41327/1‑57 (MQ=255) cgcgGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGa < 3:41327/57‑1 (MQ=255) gcgGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCCATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCCCTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTg > 7:114035/1‑149 (MQ=255) gggTGAACCCGCTCCTACGGGCGGGTTTACACGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACACAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCGGCa > 3:238643/1‑149 (MQ=37) gtgaaCCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCAc > 4:218206/4‑148 (MQ=38) tgaaCCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGa > 4:185627/3‑149 (MQ=38) tgaaCCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGa > 8:322292/3‑149 (MQ=38) tAAACCCGCCCGTAGGAGCGGGTTCAACCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAAACCGCAAGCCGTCACTACGGCTCTCAAAGCGCAGGCTAACGGCACACCGCTCCACg > 4:267233/1‑148 (MQ=39) aaaCCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGAt > 3:75222/1‑149 (MQ=255) aaaCCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGAt > 1:396110/1‑149 (MQ=255) cccgctccTACGGGCGGGTTTACCCGCGAACAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGc > 1:38526/1‑149 (MQ=38) cccgcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGc > 8:301565/1‑149 (MQ=255) ccgcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGcc > 7:27778/1‑149 (MQ=255) ccgcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGcc > 3:228241/1‑149 (MQ=255) cgcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCt > 5:351935/1‑149 (MQ=255) gTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGcaca < 1:184043/149‑1 (MQ=255) ggAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATc < 8:27778/149‑1 (MQ=255) | CGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATC > NC_002947/4796475‑4796765 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |