Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,832,060 A→T 10.8% intergenic (+35/+117) pvdS → / ← pvdY alternative sigma factor/hydroxyproline acetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,832,0600AT10.8% 172.9 / 9.2 63intergenic (+35/+117)pvdS/pvdYalternative sigma factor/hydroxyproline acetylase
Reads supporting (aligned to +/- strand):  ref base A (29/27);  new base T (2/5);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGTGCCGCAGAAGGACATTGCCAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCAGCTTGCAACGATCGAAAAAC  >  NC_002947/4831913‑4832204
                                                                                                                                                    |                                                                                                                                                
gCGTGCCGCAGAAGGACATTGCCAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGa                                                                                                                                                  <  1:298991/148‑1 (MQ=255)
gCGTGCCGCAGAAGGACATTGCCAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGa                                                                                                                                                  <  3:382015/148‑1 (MQ=255)
    gCCGCAGAAGGACATTGCCAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGct                                                                                                                                             >  1:411530/1‑149 (MQ=255)
       gCAGAAGGACATTGCCAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAAAGCGAGAGCGATCCAGGTTCGctctg                                                                                                                                          >  6:116185/1‑148 (MQ=39)
           aaGGACATTGCCAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCtt                                                                                                                                      >  3:142377/1‑149 (MQ=255)
           aaGGACATTGCCAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCtt                                                                                                                                      <  3:114702/149‑1 (MQ=255)
            aGGACATTGCCAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCttt                                                                                                                                     <  8:212502/149‑1 (MQ=255)
             ggACATTGCCAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGACCGCTCTCGCtttt                                                                                                                                    >  1:205969/1‑149 (MQ=255)
             ggACATTGCCAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGACCGAAACTGGATCGCTCACGcaat                                                                                                                                     >  2:332499/1‑145 (MQ=255)
             ggACATTGCCAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAAAGCGAGAGCGATCCAGGTTCGCTCTGGCgttt                                                                                                                                    >  5:351411/1‑149 (MQ=38)
              gACATTGCCAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGATCCAGGTTCGCTCTGGCGTttg                                                                                                                                   <  4:408192/149‑1 (MQ=39)
               aCATTGCCAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTttgc                                                                                                                                  <  3:249545/149‑1 (MQ=255)
                  ttGCCAAGGAACTGGTCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTttgcttg                                                                                                                               <  5:437457/149‑1 (MQ=255)
                     ccAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAAAGCGAGAGCGATCCAGGTTCGCTCTGGCGTTTGCTTGCa                                                                                                                             <  6:351411/148‑1 (MQ=37)
                     ccAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAAAGCGAGAGCGATCCAGGTTCGCTCTGGCGTTTGCTTGCa                                                                                                                             <  3:93824/148‑1 (MQ=37)
                     ccAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAAAGCGAGAGCGATCCAGGTTCGCGCTGGCGTTTGCTTGCa                                                                                                                             <  5:116185/148‑1 (MQ=37)
                      cAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAgc                                                                                                                           >  6:279064/1‑149 (MQ=255)
                       aaGGAACTGGGCGTATCGCCGCCCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAgcg                                                                                                                          >  2:389078/1‑149 (MQ=255)
                       aaGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAgcg                                                                                                                          <  2:345267/149‑1 (MQ=255)
                         ggAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAAAGCGAGAGCGATCCAGGTTCGCTCTGGCGTTTGCTTGCAgtcat                                                                                                                        <  3:270009/149‑5 (MQ=37)
                             cTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCGGCGCTAGTa                                                                                                                    <  4:142377/149‑1 (MQ=255)
                                ggCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATgg                                                                                                                 >  6:299088/1‑149 (MQ=255)
                                ggCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATg                                                                                                                  <  2:68858/148‑1 (MQ=255)
                                        gCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTg                                                                                                          <  2:411530/148‑1 (MQ=255)
                                         ccGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGc                                                                                                         >  7:1120/1‑148 (MQ=255)
                                         ccGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCa                                                                                                        <  7:4804/149‑1 (MQ=255)
                                              cTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCCAGTATGGTGTTTTGCAAATAc                                                                                                   >  6:370015/1‑149 (MQ=255)
                                                              aTCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCa                                                                                    <  6:379678/148‑1 (MQ=255)
                                                              aTCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCa                                                                                    <  5:299088/148‑1 (MQ=255)
                                                               tcggcgATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAg                                                                                   <  8:404102/145‑1 (MQ=255)
                                                               tCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAgc                                                                                  >  6:3028/1‑149 (MQ=255)
                                                                 cgcgATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAgcg                                                                                 <  5:279064/148‑1 (MQ=255)
                                                                 cgcgATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAgcg                                                                                 >  2:201432/1‑148 (MQ=255)
                                                                         gcTGGTGCATTGCCGCAAGACGGCCAGGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAAt                                                                                            >  1:226266/1‑130 (MQ=255)
                                                                         gcTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAAt                                                                                            <  2:226266/129‑1 (MQ=255)
                                                                           tGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAAcgcc                                                                      <  8:1120/149‑1 (MQ=255)
                                                                             gTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAAcgccg                                                                     >  6:159319/1‑148 (MQ=255)
                                                                                cATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCaa                                                                  <  1:332499/148‑1 (MQ=255)
                                                                                cATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAg                                                                 <  5:3028/149‑1 (MQ=255)
                                                                                     ccGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGACCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATg                                                            <  2:205969/149‑1 (MQ=255)
                                                                                      cGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATgg                                                           >  7:40142/1‑149 (MQ=255)
                                                                                       gCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGt                                                          >  5:121020/1‑149 (MQ=255)
                                                                                           gACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGt                                                       >  5:326529/1‑148 (MQ=255)
                                                                                               gCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTAttt                                                  <  6:173100/149‑1 (MQ=255)
                                                                                                ccAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTtg                                                 >  7:73320/1‑149 (MQ=255)
                                                                                                ccAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTtg                                                 >  1:264287/1‑149 (MQ=255)
                                                                                                          aGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAAcacc                                        <  6:326529/149‑1 (MQ=255)
                                                                                                               tGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCacac                                    >  6:144961/1‑148 (MQ=255)
                                                                                                               tGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCacac                                    >  1:222956/1‑148 (MQ=255)
                                                                                                                 aaGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTa                                  <  2:3754/148‑1 (MQ=255)
                                                                                                                 aaGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAg                                 >  6:91406/1‑149 (MQ=255)
                                                                                                                 aaGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCACTCGCTTTTGCTTGCAGCGCCAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTa                                  <  5:370015/148‑1 (MQ=255)
                                                                                                                  aGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGc                                <  1:201432/149‑1 (MQ=255)
                                                                                                                        cTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTAc                          >  6:118909/1‑149 (MQ=255)
                                                                                                                         tGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACg                         >  1:22151/1‑149 (MQ=255)
                                                                                                                            aagcaaACGCCCGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGt                        >  6:341880/1‑147 (MQ=255)
                                                                                                                            aagcaaACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCa                      <  2:437232/149‑1 (MQ=255)
                                                                                                                            aagcaaACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCa                      <  5:118909/149‑1 (MQ=255)
                                                                                                                                aaaCGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCAGCtt                  >  2:142414/1‑149 (MQ=255)
                                                                                                                                    gCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCTAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCAGCTTGc                <  2:244900/147‑1 (MQ=255)
                                                                                                                                    gCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCGAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCAGCTTGc                >  1:244900/1‑147 (MQ=255)
                                                                                                                                      cAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCAGCTTGCAAc             >  6:104552/1‑148 (MQ=255)
                                                                                                                                         agCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATAAGGCCAATAACTCGCAGCGCAAAAAACGCCGAAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCAGCTTGCAACGATc         >  2:21510/1‑149 (MQ=255)
                                                                                                                                              aCCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCAGCTTGCAACGATCGaaa     >  2:54781/1‑148 (MQ=255)
                                                                                                                                                cTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCAGCTTGCAACGATCGAAAAAc  <  8:256779/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GCGTGCCGCAGAAGGACATTGCCAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATGACTGCAAGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCAGCTTGCAACGATCGAAAAAC  >  NC_002947/4831913‑4832204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: