Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,854,775 T→A 5.2% D109V (GAT→GTT)  dnaX ← DNA polymerase III subunit gamma

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,854,7750TA 5.2% 244.9 / 2.5 77D109V (GAT→GTT) dnaXDNA polymerase III subunit gamma
Reads supporting (aligned to +/- strand):  ref base T (39/34);  new base A (2/2);  total (41/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

GGTGGTGGCGAGGATGAACTTGACGTAGGGCGGCGGCTCTTCCAGCGTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGCCTTCGTCGATTTCCCGGCACACCGAACAGGTGCCACAGGGCGTGGAGGTGATGCCGGTTTCACAGTTCAGGCACTT  >  NC_002947/4854630‑4854923
                                                                                                                                                 |                                                                                                                                                    
ggtggtGGCGAGGATGAACTTGCCGTAGGGCGGCGGCTCTTCCAGCGTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGa                                                                                                                                                   <  6:256347/149‑1 (MQ=255)
 gtggtgGCGAGGATGAACTTGACGTAGGGCGGCGGCTCTTCCAGCGTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCTCGATAACgtg                                                                                                                                                  >  3:69686/1‑147 (MQ=255)
        cGAGGATGAACTTGACGTAGGGCGGCGGCTCTTCCAGCGTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCTCGATAACGTGCAGTAcg                                                                                                                                           <  4:69686/149‑1 (MQ=255)
         gAGGATGAACTTGACGTAGGGCGGCGGCTCTTCCAGCGTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTcgc                                                                                                                                          <  4:399828/149‑1 (MQ=255)
         gAGGATGAACTTGACGTAGGGCGGCGGCTCTTCCAGCGTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTcgc                                                                                                                                          <  2:163669/149‑1 (MQ=255)
            gATGAACTTGACGTAGGGCGGCGGCTCTTCCAGCGTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAg                                                                                                                                       >  3:123075/1‑149 (MQ=255)
             accaccTTGCCGTAGGGCGGCGGCTCTTCCAGCGTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGACGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGa                                                                                                                                        <  3:376077/142‑1 (MQ=255)
              tGAACTTGACGTAGGGCGGCGGCTCTTCCAGCGTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAgtg                                                                                                                                     <  3:237951/149‑1 (MQ=255)
                aaCTTGACGTAGGGCGGCGGCTCTTCCAGCGTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAgtgt                                                                                                                                    <  4:123075/148‑1 (MQ=255)
                  cTTGACGTAGGGCGGCGGCTCTTCCAGCGTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCct                                                                                                                                 >  2:212838/1‑149 (MQ=255)
                   ttGACGTAGGGCGGCGGCTCTTCCAGCGTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTc                                                                                                                                   >  8:243176/1‑146 (MQ=255)
                   ttGACGTAGGGCGGCGGCTCTTCCAGCGTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTc                                                                                                                                   <  7:243176/146‑1 (MQ=255)
                        gTAGGGCGGCGGCTCTTCCAGCGTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATc                                                                                                                                                     >  4:157798/1‑123 (MQ=255)
                        gTAGGGCGGCGGCTCTTCCAGCGTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATc                                                                                                                                                     <  3:157798/123‑1 (MQ=255)
                            ggcggccgcGCTTCCAGCGGCTTGAGCAAGGCGTTGAATGAGTGGGGCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTgg                                                                                                                        <  7:81444/148‑1 (MQ=255)
                                    ctctTCCAGCGTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTgg                                                                                                               >  5:232197/1‑149 (MQ=255)
                                       ttCCAGCGTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCTCGATAACGTGCAGTAcg                                                                                                                                           <  8:244808/118‑1 (MQ=255)
                                       ttCCAGCGTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCTCGATAACGTGCAGTAcg                                                                                                                                           >  7:244808/1‑118 (MQ=255)
                                       ttCCAGCGTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTg                                                                                                                         <  7:234766/136‑1 (MQ=255)
                                       ttCCAGCGTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTg                                                                                                                         >  8:234766/1‑136 (MQ=255)
                                       ttCCAGCGTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTggcgg                                                                                                            >  8:260968/1‑149 (MQ=255)
                                       ttCCAGCGTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTggc                                                                                                              <  7:330455/147‑1 (MQ=255)
                                          cAGCGTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGt                                                                                                         <  6:232197/149‑1 (MQ=255)
                                          cAGCGTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGt                                                                                                         >  2:232483/1‑149 (MQ=255)
                                           aGCGTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTCGGGGCGTACTGCACGTTATCGAGCAGTTc                                                                                                                                            >  4:147338/1‑113 (MQ=255)
                                            gCGTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTggcg                                                                                                             <  6:106609/143‑1 (MQ=255)
                                            gCGTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTggcg                                                                                                             >  5:106609/1‑143 (MQ=255)
                                              gTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCAGGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGTTACGGCTGGCGGCGtcgat                                                                                                     <  8:294310/149‑1 (MQ=255)
                                                 ttGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTCGGTACGGCTGGCGGCGtcgatctc                                                                                                  >  4:109336/1‑149 (MQ=255)
                                                  tGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGtcgatctc                                                                                                  >  7:187647/1‑148 (MQ=255)
                                                    aGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGtcgatctcgat                                                                                               >  1:198503/1‑149 (MQ=255)
                                                    aGCAAGGCGGTGACTGAGGGGGTCGAGAGCATGGGCACGGCGTCGATGAGGGAGCCCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGtcgatctcgat                                                                                               <  6:363009/149‑1 (MQ=255)
                                                     gCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGtcgatctcgat                                                                                               >  3:297967/1‑148 (MQ=255)
                                                       aaGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAg                                                                                            <  2:198503/149‑1 (MQ=255)
                                                         ggCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGt                                                                                          >  3:68154/1‑149 (MQ=255)
                                                         ggCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGt                                                                                          >  2:364159/1‑149 (MQ=255)
                                                            gTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGtcgatctcgatc                                                                                              >  2:396585/1‑142 (MQ=255)
                                                            gTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGtcgatctcgatc                                                                                              <  1:396585/142‑1 (MQ=255)
                                                             ttGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCTATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACATTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTcgac                                                                                      <  7:267868/149‑1 (MQ=255)
                                                                 aTGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCGTCTACCTTGGTACGGCTGGCGGCGTCGGTCTCGATCAGGTCGACGaag                                                                                  >  4:324501/1‑148 (MQ=255)
                                                                    aGTGGGTCGAGATCATGTGAACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGTGGAGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTAGATCTCGATCAGGTCGACGAAACgg                                                                               <  4:291163/149‑1 (MQ=255)
                                                                    aGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCTTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACg                                                                                <  7:126840/148‑1 (MQ=255)
                                                                      tGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGcc                                                                             >  3:285242/1‑149 (MQ=255)
                                                                         gTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTcgc                                                                                                                                          >  8:126812/1‑85 (MQ=255)
                                                                         gTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTcgc                                                                                                                                          <  7:126812/85‑1 (MQ=255)
                                                                         gTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGCCTtc                                                                          <  1:232483/149‑1 (MQ=255)
                                                                           cGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGCCTtcgt                                                                        <  7:260968/149‑1 (MQ=255)
                                                                            gagaGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGCCTtcgt                                                                        >  4:77344/1‑148 (MQ=255)
                                                                                 cATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTc                                                                                                                                   <  8:227598/84‑1 (MQ=255)
                                                                                 cATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTc                                                                                                                                   >  7:227598/1‑84 (MQ=255)
                                                                                        aCTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGATAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCATCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGCCTTCGTCGATTTCCCGGca                                                           >  6:190700/1‑149 (MQ=255)
                                                                                          ttCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGCCTTCGTCGATTTCCCGGc                                                            >  6:421628/1‑146 (MQ=255)
                                                                                          ttCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGCCTTCGTCGATTTCCCGGc                                                            <  5:421628/146‑1 (MQ=255)
                                                                                               cgATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGCCTTCGTCGATTTCCCGGCACACCGaa                                                    >  8:154292/1‑149 (MQ=255)
                                                                                                     ggTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGCCTTCGTCGATTTCCCGGCACACCGAACAGGTg                                              >  1:345616/1‑149 (MQ=255)
                                                                                                      gTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGCCTTCGTCGATTTCCCGGCACACCGAACAGGTGc                                             >  5:168704/1‑149 (MQ=255)
                                                                                                      gTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGCCTTCGTCGATTTCCCGGCACACCGAACAGGTGc                                             >  4:151945/1‑149 (MQ=255)
                                                                                                         gACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGCCGTCGTCGATTTCCCGGCACACCGAACAGGTGCca                                           >  6:18742/1‑148 (MQ=255)
                                                                                                           ccTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGCCTTCGTCGATTTCCCGGCACACCGAACAGGTGCCACAg                                        >  6:378087/1‑149 (MQ=255)
                                                                                                            cTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGTCCTTCGTCGATTTCCCGGCACACCGAACAGGTGCCACAgg                                       >  6:375194/1‑149 (MQ=255)
                                                                                                            cTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGCCTTCGTCGATTTCCCg                                                              <  8:131061/126‑1 (MQ=255)
                                                                                                            cTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGCCTTCGTCGATTTCCCg                                                              >  7:131061/1‑126 (MQ=255)
                                                                                                               gAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTCGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGCCTTCGTCGATTTCCCGGCACACCGAACAGGTGCCACAGGGc                                     <  3:109336/148‑1 (MQ=255)
                                                                                                                aaGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGAGTCGATCTCGATCAGGTCGACGAAACTGCCTACGTCGATTTCCCGGCACAACGAACAGGTGCCACAGGGCGt                                   >  8:255901/1‑149 (MQ=255)
                                                                                                                 aGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGCCTTCGTCGATTTCCCGGCACACCGAACAGGTGCCACAGGGCGt                                   <  2:345616/148‑1 (MQ=255)
                                                                                                                    gcCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGCCTTCGTCGATTTCCCGGCACACCGAACAGGTGCCACAGGGCGTGGAg                               <  3:151945/149‑1 (MQ=255)
                                                                                                                    gcCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGCCTTCGTCGATTTCCCGGCACACCGAACAGGTGCCACAGGGCGTGGAg                               <  8:187647/149‑1 (MQ=255)
                                                                                                                      ccACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAgg                                                                                           >  2:225/1‑87 (MQ=255)
                                                                                                                      ccACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAgg                                                                                           <  1:225/87‑1 (MQ=255)
                                                                                                                            cTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGCCTTCGTCGATTTCCCGGCACACCGAACAGGTGCCACAGGGCGTGGAGGTGATGcc                       >  7:34034/1‑149 (MQ=255)
                                                                                                                               ggggCGTACTGCACGTTATCGAGCAGTTCGAGAGTGTCCTCTACCAAGGTAAGGCTGCCGTCGTCGATCTCGATAAGGTCGACGAAACGGCCTTAGTCGATTTCCCGGCACACCCAACAGGTGCAAAAGGGCGTGGAGGTGATGCCGGt                    >  2:335036/1‑149 (MQ=255)
                                                                                                                                  gCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGCCTTCGTCGATTTCCCGGCACACCGAACAGGTGCCACAGGGCGTGGAGGTGATGCCGGttt                  >  7:401282/1‑148 (MQ=255)
                                                                                                                                    gTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGCCTTCGTCGATTTCCCGGCACACCGAACAGGTGCCACAGGGCGTGGAGGTGATGCCGGTTTcac               <  7:254329/149‑1 (MQ=255)
                                                                                                                                     tACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGCCTTCGTCGATTTCCCGGCACACCGAACAGGTGCCACAGGGCGTGGAGGTGATGCCGGTTTcaca              <  5:190700/149‑1 (MQ=255)
                                                                                                                                      aCTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGCCTTCGTCGATTTCCCGGCACACCGAACAGGTGCCACAGGGCGTGGAGGTGATGCCGGTTTCACAg             >  1:223806/1‑149 (MQ=255)
                                                                                                                                          cACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGCCTTCGTCGATTTCCCGGCACACCGAACAGGTGCCACAGGGCGTGGAGGTGATGCCGGTTTCACAGTTCa         <  2:223806/149‑1 (MQ=255)
                                                                                                                                              ttATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGCCTTCGTCGATTTCCCGGCACACCGAACAGGTGCCACAGGGCGTGGAGGTGATGCCGGTTTCACAGTTCAGGCa     >  1:85493/1‑149 (MQ=255)
                                                                                                                                                 tCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGCCTTCGTCGATTTCCCGGCACACCGAACAGGTGCCACAGGGCGTGGAGGTGATGCCGGTTTCACAGTTCAGGCACtt  >  3:355801/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GGTGGTGGCGAGGATGAACTTGACGTAGGGCGGCGGCTCTTCCAGCGTCTTGAGCAAGGCGTTGAATGAGTGGGTCGAGAGCATGTGCACTTCGTCGATCAGGTAGACCTTGAAGCGCCCACGGCTAGGGGCGTACTGCACGTTATCGAGCAGTTCGCGAGTGTCCTCTACCTTGGTACGGCTGGCGGCGTCGATCTCGATCAGGTCGACGAAACGGCCTTCGTCGATTTCCCGGCACACCGAACAGGTGCCACAGGGCGTGGAGGTGATGCCGGTTTCACAGTTCAGGCACTT  >  NC_002947/4854630‑4854923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑