BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,860,813	G→C	5.3%	L79L (CTC→CTG)	ligA ←	DNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,860,813	0	G	C	5.3%	368.6 / 7.0	112	L79L (CTC→CTG)	ligA	DNA ligase
Reads supporting (aligned to +/- strand): ref base G (58/47); new base C (3/3); total (61/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

Only 100 of 112 total aligned reads displayed.

CTGGCCGTCGCGGTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTCGGCAT  >  NC_002947/4860666‑4860958

  ggCCGTCGCGGTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTTGGCTTCTTCGAAGGCGTTGCCCAGGCTGAg                                                                                                                                                 >  8:104752/1‑148 (MQ=255)

    ccGTCGCGGTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCAt                                                                                                                                              <  7:310634/149‑1 (MQ=255)

     cGTCGCGGTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATg                                                                                                                                             >  4:363922/1‑149 (MQ=255)

      gTCGCGGTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATgg                                                                                                                                            <  6:222554/149‑1 (MQ=255)

      gTCGCGGTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATgg                                                                                                                                            <  8:228360/149‑1 (MQ=255)

       tCGCGGTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATggg                                                                                                                                           >  8:322237/1‑149 (MQ=255)

          cgGTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCATGCTCAGCCTGGGCaa                                                                                                                                        <  7:381465/149‑2 (MQ=255)

           ggTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACt                                                                                                                                       >  3:255312/1‑149 (MQ=255)

               cagcagGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGt                                                                                                                                   <  1:379073/149‑1 (MQ=255)

                agcagGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGGTTCTTCGAAGGCGTTGCCCAGGCTTAGCATGGGCACTTCGTg                                                                                                                                  <  8:232901/149‑1 (MQ=255)

                   agGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCg                                                                                                                               <  2:109007/149‑1 (MQ=255)

                    ggCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCg                                                                                                                               >  3:415128/1‑148 (MQ=255)

                       tCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCATGCTCAGCCTGGGCaacg                                                                                                                                      >  3:241433/1‑135 (MQ=255)

                       tCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTAcc                                                                                                                           >  6:77027/1‑149 (MQ=255)

                       tCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTAc                                                                                                                            >  8:346155/1‑148 (MQ=255)

                        cACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCt                                                                                                                          <  4:255312/149‑1 (MQ=255)

                        cACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCt                                                                                                                          <  8:346191/149‑1 (MQ=255)

                         aCGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTg                                                                                                                         >  3:240133/1‑149 (MQ=255)

                          cGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTgg                                                                                                                        <  8:189243/149‑1 (MQ=255)

                          cGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTgg                                                                                                                        <  6:230460/149‑1 (MQ=255)

                                ggccaTCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTTGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAAc                                                                                                                  <  7:104752/149‑1 (MQ=255)

                                   caTCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAAcgcc                                                                                                               >  7:311119/1‑149 (MQ=255)

                                    aTCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAAcgccg                                                                                                              >  7:399891/1‑149 (MQ=255)

                                    aTCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAAcgccg                                                                                                              >  5:198474/1‑149 (MQ=255)

                                    aTCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAAcgccg                                                                                                              >  6:169609/1‑149 (MQ=255)

                                         gCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTgg                                                                                                                                 >  3:48429/1‑125 (MQ=255)

                                         gCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAg                                                                                                         >  5:65330/1‑149 (MQ=255)

                                          cTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAgg                                                                                                        <  7:346155/149‑1 (MQ=255)

                                           ttGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAAcgc                                                                                                                <  2:54359/140‑1 (MQ=255)

                                           ttGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAAcgc                                                                                                                >  1:54359/1‑140 (MQ=255)

                                            tGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCAt                                                                                                                                              >  8:393550/1‑109 (MQ=255)

                                            tGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTCAGCAt                                                                                                                                              <  7:393550/109‑1 (MQ=255)

                                              ggTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAg                                                                                                     <  5:151605/148‑1 (MQ=255)

                                                ttCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTg                                                                                                                                  <  2:230420/117‑1 (MQ=255)

                                                ttCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTg                                                                                                                                  >  1:230420/1‑117 (MQ=255)

                                                ttCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTc                                                                                                  >  6:376984/1‑149 (MQ=255)

                                                   gCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAg                                                                                                     <  1:62293/143‑1 (MQ=255)

                                                   gCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAg                                                                                                     >  2:62293/1‑143 (MQ=255)

                                                        tGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCcaca                                                                                          >  3:144907/1‑149 (MQ=255)

                                                            gTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCt                                                                                      >  5:420358/1‑149 (MQ=255)

                                                            gTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCACCAAGGCAGCTCCACCCACACGCt                                                                                      <  4:30518/149‑1 (MQ=255)

                                                             tCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCtg                                                                                     <  6:198474/149‑1 (MQ=255)

                                                             tCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCtg                                                                                     >  4:58697/1‑149 (MQ=255)

                                                             tCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCtg                                                                                     >  7:53205/1‑149 (MQ=255)

                                                               gACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCtggg                                                                                   >  4:14570/1‑149 (MQ=255)

                                                               gACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCtgg                                                                                    <  4:415128/148‑1 (MQ=255)

                                                               gACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCtgg                                                                                    <  4:144907/148‑1 (MQ=255)

                                                                aCCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCtgggt                                                                                  <  6:65330/149‑1 (MQ=255)

                                                                aCCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCtgggt                                                                                  <  3:58697/149‑1 (MQ=255)

                                                                aCCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCt                                                                                      >  4:25640/1‑145 (MQ=255)

                                                                 ccGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCtgggtg                                                                                 >  1:100660/1‑149 (MQ=255)

                                                                  cgcgCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCtgggtgg                                                                                <  2:83741/149‑1 (MQ=255)

                                                                  cgcgCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCtgggtg                                                                                 >  5:49508/1‑148 (MQ=255)

                                                                  cgcgCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACACACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCtgggtgg                                                                                >  1:301405/1‑149 (MQ=255)

                                                                      cccGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGa                                                                            <  3:14570/149‑1 (MQ=255)

                                                                      cccGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGa                                                                            <  8:311119/149‑1 (MQ=255)

                                                                          gCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCg                                                                        >  5:344924/1‑149 (MQ=255)

                                                                           cTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCgg                                                                       <  5:169609/149‑1 (MQ=255)

                                                                              aTCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCggggg                                                                    <  5:77027/149‑1 (MQ=255)

                                                                              aTCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCgggg                                                                     <  3:363922/148‑1 (MQ=255)

                                                                                  aGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTAc                                                                >  5:58435/1‑149 (MQ=255)

                                                                                     ccTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAg                                                             <  4:77053/149‑1 (MQ=255)

                                                                                            ccACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCgg                                                       <  5:376984/148‑1 (MQ=255)

                                                                                             cACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGgt                                                     >  2:157021/1‑149 (MQ=255)

                                                                                               cgcgACGGCCGAACTCACGCAGGTCGGCTTCTTCGTAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCATCTCCACCCACACGCTGGGTGTGCGAGTCGTGGGTTACCAGGTGCGGgtgt                                                   <  8:303591/149‑1 (MQ=255)

                                                                                                gcgACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGtt                                                  >  1:397617/1‑149 (MQ=255)

                                                                                                     ggCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTCGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCt                                             >  7:390264/1‑149 (MQ=255)

                                                                                                      gCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCtt                                            >  4:32549/1‑149 (MQ=255)

                                                                                                        cGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGt                                                           >  8:302998/1‑132 (MQ=255)

                                                                                                        cGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTc                                           >  6:198152/1‑148 (MQ=255)

                                                                                                        cGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTc                                           >  7:289866/1‑148 (MQ=255)

                                                                                                           aCTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCtgggt                                                                                  <  4:350738/106‑1 (MQ=255)

                                                                                                           aCTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCtgggt                                                                                  >  3:350738/1‑106 (MQ=255)

                                                                                                             tCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAgcgc                                     >  8:280259/1‑149 (MQ=255)

                                                                                                             tCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAgcgc                                     >  8:280264/1‑149 (MQ=255)

                                                                                                               aCGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCt                                   >  4:1875/1‑149 (MQ=255)

                                                                                                                 gCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCtt                                  >  5:408816/1‑148 (MQ=255)

                                                                                                                 gCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCtt                                  >  8:195533/1‑148 (MQ=255)

                                                                                                                 gCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTg                                 <  7:280259/149‑1 (MQ=255)

                                                                                                                 gCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTg                                 <  7:280264/149‑1 (MQ=255)

                                                                                                                     gTCGGCTTCTTCGAAGGCATTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCCCCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCt                             >  3:93433/1‑149 (MQ=255)

                                                                                                                          cttcttCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTg                        <  1:204445/149‑1 (MQ=255)

                                                                                                                           ttcttcGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTg                        >  5:148544/1‑148 (MQ=255)

                                                                                                                             cttcGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGaa                      <  7:195533/148‑1 (MQ=255)

                                                                                                                                cGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAgg                  >  5:385963/1‑149 (MQ=255)

                                                                                                                                 gAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAgg                  <  2:100660/148‑1 (MQ=255)

                                                                                                                                 gAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGc                 >  7:92534/1‑149 (MQ=255)

                                                                                                                                   aGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCgg               >  4:50181/1‑149 (MQ=255)

                                                                                                                                     gaagtGCCCATGCTCAGCCTGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTAAACAGGCGGTc             >  1:152407/5‑149 (MQ=255)

                                                                                                                                       gTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCgggg                                                                     >  8:210170/1‑91 (MQ=255)

                                                                                                                                       gTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCgggg                                                                     <  7:210170/91‑1 (MQ=255)

                                                                                                                                       gTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCg            >  3:245933/1‑148 (MQ=255)

                                                                                                                                        ttGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCtt                                  >  1:159728/1‑125 (MQ=255)

                                                                                                                                        ttGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCtt                                  <  2:159728/125‑1 (MQ=255)

                                                                                                                                        ttGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTa          <  4:245933/149‑1 (MQ=255)

                                                                                                                                           cccAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTc       >  6:365918/1‑149 (MQ=255)

                                                                                                                                           cccAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTc       >  4:4950/1‑149 (MQ=255)

                                                                                                                                            ccAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTCg      >  8:331873/1‑149 (MQ=255)

                                                                                                                                             cAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTCgg     <  6:344924/149‑1 (MQ=255)

                                                                                                                                                gCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTCGGCAt  >  2:179211/1‑149 (MQ=255)

CTGGCCGTCGCGGTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGCTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTCGGCAT  >  NC_002947/4860666‑4860958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`