BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,918,174	C→G	6.4%	L191L (CTC→CTG)	PP_4328 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,918,174	0	C	G	6.4%	330.1 / 9.3	105	L191L (CTC→CTG)	PP_4328	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (48/50); new base G (2/5); total (50/55)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 105 total aligned reads displayed.

ACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTCACCCGCGACCCAGGCGTCGATGGCGACCTGCGCGCCAGCGCCGAACG  >  NC_002947/4918027‑4918320

aCCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCa                                                                                                                                                   <  8:27248/149‑1 (MQ=255)

   ggCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCg                                                                                                                                                >  8:249789/1‑149 (MQ=255)

     ccGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCggc                                                                                                                                              <  8:179553/149‑1 (MQ=255)

      cGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGGTCAGTCATTGCGCTTCGTGCCGCTGAGCggca                                                                                                                                             <  8:217346/149‑1 (MQ=255)

      cGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTGAGCggca                                                                                                                                             <  7:206688/149‑1 (MQ=255)

      cGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCggca                                                                                                                                             <  6:201971/149‑1 (MQ=255)

      cGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCggca                                                                                                                                             <  7:131300/149‑1 (MQ=255)

         gtACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCggcaggc                                                                                                                                          <  4:279072/149‑1 (MQ=255)

          tACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTGAGCggcacga                                                                                                                                          <  4:221027/148‑4 (MQ=255)

          tACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCggcaggc                                                                                                                                          <  1:68733/148‑1 (MQ=255)

            ccGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTGAGCggcacgaagc                                                                                                                                       <  7:389132/149‑7 (MQ=255)

             cGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGa                                                                                                                                      >  4:93139/1‑149 (MQ=255)

             cGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGa                                                                                                                                      >  4:26304/1‑149 (MQ=255)

                gcgcTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCa                                                                                                                                   <  7:19059/149‑1 (MQ=255)

                gcgcTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCCCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGAcc                                                                                                                                    >  4:282386/1‑148 (MQ=255)

                 cgcTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCaa                                                                                                                                  <  6:178344/149‑1 (MQ=255)

                 cgcTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCaa                                                                                                                                  >  3:385475/1‑149 (MQ=255)

                   cTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACt                                                                                                                                >  2:233101/1‑149 (MQ=255)

                    tGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGAGCTTCGTGCCGCTCAGCGGATGGCAGGACCAACt                                                                                                                                >  4:304408/1‑148 (MQ=255)

                      gTCAGGCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGGTGGTCATTGGCAGCCTGCTCAGCGACCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCa                                                                                                                              <  8:256791/148‑1 (MQ=255)

                      gTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGGAAGGATAAGTCATTTAGCTTAGTGACCCTCAGCGCGCGGCAGGGCCaaaa                                                                                                                                >  1:141803/1‑146 (MQ=255)

                       tCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAg                                                                                                                             >  4:254842/1‑148 (MQ=255)

                          gCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCAtt                                                                                                                         >  1:145315/1‑149 (MQ=255)

                          gCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCAtt                                                                                                                         >  3:232215/1‑149 (MQ=255)

                           ccTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTg                                                                                                                        >  3:118221/1‑149 (MQ=255)

                             tGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGcc                                                                                                                      >  1:201420/1‑149 (MQ=255)

                             tGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGAGGCAGGCAGGACCAACTCAGCATTGcc                                                                                                                      >  7:78723/1‑149 (MQ=255)

                               cTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCa                                                                                                                    <  7:222259/149‑1 (MQ=255)

                                  aaCAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACa                                                                                                                 <  7:157442/149‑1 (MQ=255)

                                  aaCAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACa                                                                                                                 >  4:313794/1‑149 (MQ=255)

                                      gCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑GGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAgctg                                                                                                             <  6:341025/149‑1 (MQ=255)

                                       cAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAgctgc                                                                                                            <  3:313794/149‑1 (MQ=255)

                                           cAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGAc                                                                                                        >  4:39905/1‑149 (MQ=255)

                                           cAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGAc                                                                                                        <  4:260765/149‑1 (MQ=255)

                                           cAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGAAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGAc                                                                                                        <  7:171779/149‑1 (MQ=255)

                                            aGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGAcc                                                                                                       <  3:93139/149‑1 (MQ=255)

                                             ggCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGAccc                                                                                                      <  1:31219/149‑1 (MQ=255)

                                              gCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCa                                                                                                     <  3:166404/149‑1 (MQ=255)

                                               ccGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAg                                                                                                    <  1:339866/149‑1 (MQ=255)

                                                cGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGc                                                                                                   <  3:282386/149‑1 (MQ=255)

                                                  gTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCaa                                                                                                 <  3:26076/149‑1 (MQ=255)

                                                     ccACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGTG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCaaaa                                                                                               <  8:78723/148‑1 (MQ=255)

                                                     ccACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAAc                                                                                              >  3:377226/1‑149 (MQ=255)

                                                       acacTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCg                                                                                            >  7:218494/1‑149 (MQ=255)

                                                        cacTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGc                                                                                           >  7:292941/1‑149 (MQ=255)

                                                        cacTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGc                                                                                           <  8:218494/149‑1 (MQ=255)

                                                         acTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACt                                                                                                                                >  3:37596/1‑111 (MQ=255)

                                                         acTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACt                                                                                                                                <  4:37596/111‑1 (MQ=255)

                                                         acTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGc                                                                                           >  2:283994/1‑148 (MQ=255)

                                                           tGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGcc                                                                                          >  7:101326/1‑147 (MQ=255)

                                                                aTCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAAc                                                                                              >  2:307275/1‑138 (MQ=255)

                                                                aTCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTc                                                                                   <  4:232215/149‑1 (MQ=255)

                                                                aTCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGCCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAAc                                                                                              <  1:307275/138‑1 (MQ=255)

                                                                 tCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCg                                                                                  <  4:385475/149‑1 (MQ=255)

                                                                   gACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTCATGACCCAGCAAAACCGCCAAGCCTCGc                                                                                 >  7:380155/1‑148 (MQ=255)

                                                                      aGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTAcc                                                                             >  2:147536/1‑149 (MQ=255)

                                                                         ccgcgcccgcTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCgg                                                                          >  3:61065/1‑149 (MQ=255)

                                                                          cgcgcccgcTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGc                                                                         <  4:118221/149‑1 (MQ=255)

                                                                           gcgcccgcTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGcc                                                                        >  2:237395/1‑149 (MQ=255)

                                                                            cgcccgcTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCt                                                                       <  2:141803/149‑1 (MQ=255)

                                                                             gcccgcTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTg                                                                      >  5:181175/1‑149 (MQ=255)

                                                                              cccgcTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGc                                                                     <  6:372102/149‑1 (MQ=255)

                                                                               ccgcTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCt                                                                    >  3:229389/1‑149 (MQ=255)

                                                                               ccgcTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCt                                                                    >  4:374637/1‑149 (MQ=255)

                                                                                     gTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGc                                                              >  1:437110/1‑149 (MQ=255)

                                                                                      tCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCAGCTCAACGcc                                                             >  8:143261/1‑149 (MQ=255)

                                                                                        aTTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGccc                                                            <  3:1131/148‑1 (MQ=255)

                                                                                        aTTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCt                                                           >  8:211583/1‑149 (MQ=255)

                                                                                         ttGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTg                                                          <  3:304408/149‑1 (MQ=255)

                                                                                         ttGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTg                                                          <  3:374637/149‑1 (MQ=255)

                                                                                              aGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACa                                                     <  6:181175/149‑1 (MQ=255)

                                                                                               gCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAg                                                    <  4:377226/149‑1 (MQ=255)

                                                                                                    cTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTc                                                 <  5:295570/147‑1 (MQ=255)

                                                                                                       aGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTCAccc                                             <  2:201420/148‑1 (MQ=255)

                                                                                                        gcgcCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTCACCcgc                                           >  2:75864/1‑149 (MQ=255)

                                                                                                            ccTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTCACCCGCGAcc                                       >  7:190380/1‑149 (MQ=255)

                                                                                                            ccTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTCACCCGCGAcc                                       >  8:39076/1‑149 (MQ=255)

                                                                                                                gTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTCACCCGCGACCCa                                     <  7:39076/147‑1 (MQ=255)

                                                                                                                            aTCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTCACCCGCGACCCAGGCGTCGATgg                          <  7:143261/148‑1 (MQ=255)

                                                                                                                            aTCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTCACCCGCGACCCAGGCGTCGATGGc                         <  1:75864/149‑1 (MQ=255)

                                                                                                                            aTCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTCACCCGCGACCCAGGCGTCGATGGc                         >  8:245027/1‑149 (MQ=255)

                                                                                                                              cAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTCACCCGCGACCCAGGCGTCGATGGCGa                       >  3:265355/1‑149 (MQ=255)

                                                                                                                               aGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTCACCCGCGACCCAGGCGTCGATGGCGAc                      <  8:190380/149‑1 (MQ=255)

                                                                                                                                 tCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTCACCCGCGACCCAGGCGTCGATGGCGACCt                    >  8:148738/1‑149 (MQ=255)

                                                                                                                                 tCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTCACCCGCGACCCAGGCGTCGATGGCGACCt                    <  3:254842/149‑1 (MQ=255)

                                                                                                                                    ttGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTCACCCGCGACCCAGGCGTCGATGGCGACCTgcg                 <  1:283994/149‑1 (MQ=255)

                                                                                                                                      gcgcTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTCACCCGCGACCCAGGCGTCGATGGCGACCTgcgcg               >  6:319468/1‑149 (MQ=255)

                                                                                                                                       cgcTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTCACCCGCGACCCAGGCGTCGATGGCGACCTgcgcgc              <  3:26304/149‑1 (MQ=255)

                                                                                                                                         ctgcctGCCGCTGAGCGGCAGGCAGGACCAACTCAGCAGTGCCCAACAGCTGCTGCCCCAGCAAAACCGCCAAGCCTCACTACACGGCCTGCTCCACGCCCTGCAACCGCTCACCCGCGCCCCCGGCGTCGATGGCGCCCTGCGCGCCa            >  7:280554/6‑149 (MQ=255)

                                                                                                                                         cTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTCACCCGCGACCCAGGCGTCGATGGCGACCTgcgcgc              >  3:130012/1‑147 (MQ=255)

                                                                                                                                         cTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTCACCCGCGACCCAGGCGTCGATGGCGACCTgcgcgc              >  4:301271/1‑147 (MQ=255)

                                                                                                                                         cTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTCACCCGCGACCCAGGCGTCGATGGCGACCTGCGCGCCa            <  4:371572/149‑1 (MQ=255)

                                                                                                                                          tgcctGCCGCTGAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTCACCCGCGACCCAGGCGTCGATGGCGACCTGCGCGCCAg           >  4:128052/5‑149 (MQ=255)

                                                                                                                                             gTGCCGCTCAGTGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTCACCCGCGACCCAGGCGTCGATGGCGACCTGCGCGCCAgcgc        >  1:111358/1‑149 (MQ=255)

                                                                                                                                              tGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTCACCCGCGACCCAGGCGTCGATGGCGACCTGCGCGCCAGCGcc       >  2:232724/1‑149 (MQ=255)

                                                                                                                                              ggCCGCGCAGCGGCAGGCAGGACCAACTCAGCATTCCCTTTCAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTCACCCGCGACCCAGGCGTCGATGGCGACCTGCGCGCCAGCGcc       <  1:147536/148‑1 (MQ=255)

                                                                                                                                                  gCTGAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTCACCCGCGACCCAGGCGTCGATGGCGACCTGCGCGCCAGCGCCGAAc   <  3:128052/149‑1 (MQ=255)

                                                                                                                                                  gCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCTCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTCACCCGCGACCCAGGCGTCGATGGCGACCTGCGCGCCAGCGCCGAAc   <  2:437110/149‑1 (MQ=255)

                                                                                                                                                  gCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTCACCCGCGACCCAGGCGTCGATGGCGACCTGCGCGCCAGCGCCGAAc   >  6:203360/1‑149 (MQ=255)

                                                                                                                                                   cTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTCACCCGCGACCCAGGCGTCGATGGCGACCTGCGCGCCAGCGCCGAACg  >  8:85854/1‑149 (MQ=255)

ACCGGCCGTGTACCGGGCGCTGGTCAGCCTGCTCAACAGCAACCAGGCCGGTGCCACACTGAGCATCGACAGCCCGCGCCCGCTGGTCATTGGCAGCCTGCTCAGCGCCCTGGTGCAGGG‑‑TGATCAGTCATTGCGCTTCGTGCCGCTCAGCGGCAGGCAGGACCAACTCAGCATTGCCCAACAGCTGCTGACCCAGCAAAACCGCCAAGCCTCGCTACCCGGCCTGCTCAACGCCCTGCAACAGCTCACCCGCGACCCAGGCGTCGATGGCGACCTGCGCGCCAGCGCCGAACG  >  NC_002947/4918027‑4918320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`