| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,945,329 | G→T | 11.8% | intergenic (+689/+109) | PP_5673 → / ← flhB | D‑alanine‑‑D‑alanine ligase/flagellin export apparatus substrate specificity protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,945,329 | 0 | G | T | 11.8% | 95.3 / 5.0 | 34 | intergenic (+689/+109) | PP_5673/flhB | D‑alanine‑‑D‑alanine ligase/flagellin export apparatus substrate specificity protein |
| Reads supporting (aligned to +/- strand): ref base G (22/8); new base T (2/2); total (24/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01 | |||||||||||
CGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGG > NC_002947/4945192‑4945470 | ggcccccCGGGCGGCGAATCGCGACAATGCTGGATCTTACTTTTGTTTCGGCCCTTTTATGCCTGGGTTTTTTACGCGCTACCGCTTTCGCTTACACCTCGATATTCCGCGGAAAAAACATGGGGGTCGCGCGCGCCGGGCACaggagg < 4:395399/148‑1 (MQ=255) tgctcgctcCTACATTTTTTTCGGGCCTGTTATTCCTGCGGTATTTACGCGCGACCGCCTTCGCTTACACCTCGATTTTGCGCCGTACAAACAAGGCGGTGGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGgagcg < 2:314784/148‑1 (MQ=255) ggtcggtcGTACATTTGTTTCGGGCCTGTTATTCCTGCGTGATTTACGCGCTACCGCCTTCGCTTAAACATCGATATTGCGCCGTACAAAAAAGGCGGTCGGGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGgagcga < 6:435507/147‑1 (MQ=255) aCATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTcgc > 2:361337/1‑149 (MQ=255) aTTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGa > 3:4421/1‑149 (MQ=255) ttGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATg > 1:14730/1‑149 (MQ=255) tttCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATgcgc > 3:282946/1‑149 (MQ=255) ggCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGG‑CCGAAACAAACGTAGgagc > 3:78486/1‑124 (MQ=2) aGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCgcgggc > 8:69217/1‑149 (MQ=255) gTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCgcgggcg > 8:108457/1‑149 (MQ=255) aTTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGgcggcg > 1:430151/1‑149 (MQ=255) aTTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGgcggcg > 5:14292/1‑149 (MQ=255) gcgcgACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCaa > 7:120734/1‑149 (MQ=255) ccTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAAcc > 4:182310/1‑148 (MQ=255) cgcgTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTAt < 7:108457/147‑1 (MQ=255) gcgTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTAACAGGCAACGCAAACCTATCGc > 3:274036/1‑149 (MQ=255) gTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGcc > 2:293995/1‑148 (MQ=255) gTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGcc > 7:128245/1‑148 (MQ=255) cTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAgg > 5:406132/1‑124 (MQ=255) cTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAgg < 6:406132/124‑1 (MQ=255) cTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGaa > 2:33024/1‑145 (MQ=255) tCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTacccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacct < 7:247375‑M2/148‑67 (MQ=255) cgcCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTcg > 4:28732/1‑149 (MQ=255) gcCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCCACTGTcgc > 8:402383/1‑149 (MQ=255) ccGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTcgcg < 2:14730/149‑1 (MQ=255) tACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTacccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgcc < 1:214523‑M2/149‑82 (MQ=255) aaacaaGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAg > 6:375728/1‑149 (MQ=255) caaGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGtg > 4:119739/1‑148 (MQ=255) aaGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTcc > 7:128246/1‑149 (MQ=255) cGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGgcgc > 1:52748/1‑139 (MQ=255) cGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGgcgc < 2:52748/139‑1 (MQ=255) gcgcgcgcCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATc < 8:128245/149‑1 (MQ=255) gcgcgcgaCTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTacccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtccactcaaaattcc > 3:193720‑M2/1‑52 (MQ=255) gcgcgcCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCgg > 1:250049/1‑149 (MQ=255) | CGCCGCCCGCGCGGCGCATCGCGAGCAAGCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGG > NC_002947/4945192‑4945470 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |