Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	374,742	C→G	5.2%	A11G (GCC→GGC)	PP_0312 →	electron transfer flavoprotein subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	374,742	0	C	G	5.2%	317.9 / 5.6	97	A11G (GCC→GGC)	PP_0312	electron transfer flavoprotein subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (55/37);  new base G (3/2);  total (58/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.82e-01

GCCGCGCCCACAGATCAAGGACTTGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGCAT  >  NC_002947/374594‑374889                                                                                                                                                      |                                                                                                                                                      gCCGCGCCCACAGATCAAGGACTTGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACgcgc                                                                                                                                                       >  4:148841/1‑149 (MQ=255)      gcCCACAGATCAAGGACTTGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGt                                                                                                                                                  <  1:268510/149‑1 (MQ=255)          acaGATCAAGGACTTGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGAt                                                                                                                                             >  4:90934/1‑149 (MQ=255)          acaGATCAAGGACTTGGCCGAACTGGTGGCCGACGTGAAGATCTAGGAGGACGCATAAACATTAAAAAAAAAAAAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGAt                                                                                                                                             >  2:3876/1‑149 (MQ=255)              aTCAAGGACTTGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAAGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGcc                                                                                                                                         >  8:382031/1‑149 (MQ=255)              aTCAAGGACTTGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACATGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCCCTCGGCGCGT‑GGATCGcg                                                                                                                                         <  4:84882/149‑2 (MQ=255)               tCAAGGACTTGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACTCGGCGCGT‑GGATCGcgg                                                                                                                                        <  6:146350/149‑3 (MQ=255)               tCAAGGACTTGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGccc                                                                                                                                        <  8:223725/149‑1 (MQ=255)                cAAGGACTTGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCg                                                                                                                                       >  3:202964/1‑149 (MQ=255)                  aGGACTTGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCGCATCCACGCGCCGAGT‑GGATCGCCCGt                                                                                                                                      <  1:3876/149‑1 (MQ=255)                    gACTTGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAAc                                                                                                                                   <  5:111843/149‑1 (MQ=255)                       ttGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCg                                                                                                                                 >  8:186343/1‑148 (MQ=255)                          gCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCt                                                                                                                              <  2:271644/148‑1 (MQ=255)                          gCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCAAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCt                                                                                                                              <  5:183599/148‑1 (MQ=255)                            cGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCa                                                                                                                           <  2:155115/149‑1 (MQ=255)                              aaCTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCatc                                                                                                                         >  5:318638/1‑147 (MQ=255)                                 tggtggCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGc                                                                                                                      >  3:5054/1‑149 (MQ=255)                                   gtggCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTg                                                                                                                    <  3:257450/149‑1 (MQ=255)                                    tggCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGc                                                                                                                   <  5:338784/149‑1 (MQ=255)                                        cGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGc                                                                                                               >  7:348624/1‑149 (MQ=255)                                        cGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGc                                                                                                               <  3:148841/149‑1 (MQ=255)                                             tGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGCTGCACTGATTAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑Ga                                                                                                         <  8:147254/149‑1 (MQ=255)                                              gCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GAt                                                                                                        >  3:325634/1‑149 (MQ=255)                                                 gATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCAGCG‑ATCCACGCGCCGAGT‑‑GATCGCCCGTAACCGTCTGCAACCGCTGCATGCCGC‑GATGCAg                                                                                                    >  3:193909/1‑149 (MQ=255)                                                   tCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGa                                                                                                   <  3:263813/149‑1 (MQ=255)                                                    cGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGccc                                                                                                                                        >  7:411561/1‑112 (MQ=255)                                                    cGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGccc                                                                                                                                        <  8:411561/112‑1 (MQ=255)                                                     gaggagGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGAc                                                                                                  <  7:186343/148‑1 (MQ=255)                                                     gaggagGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCA‑TCGCGGCATGCAGCGGGTGCAGACg                                                                                                 <  6:318638/149‑1 (MQ=255)                                                      aggaggACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACg                                                                                                 >  7:346058/1‑148 (MQ=255)                                                      aggaggACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAAGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGc                                                                                                <  7:382031/149‑1 (MQ=255)                                                       ggaggaCGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGc                                                                                                >  2:352470/1‑148 (MQ=255)                                                        gaggaCGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATg                                                                                                                <  8:177547/132‑1 (MQ=255)                                                        gaggaCGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATg                                                                                                                >  7:177547/1‑132 (MQ=255)                                                        gaggaCGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTCAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCaa                                                                                              <  3:219725/149‑1 (MQ=255)                                                           gaCGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAg                                                                                           >  2:183116/1‑149 (MQ=255)                                                              gCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGAc                                                                                         >  1:138547/1‑148 (MQ=255)                                                              gCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGAc                                                                                         >  2:405902/1‑148 (MQ=255)                                                               cATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGAc                                                                                         >  1:241497/1‑147 (MQ=255)                                                                 tCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGc                                                                                     <  2:241497/149‑1 (MQ=255)                                                                     atGTCCCAAAGCCGCAAACGGCTAAACTTCAACCTGCGGAGGTCCACTGATCAGCGACATTATACGACGCG‑ATCCACGCGCCGAGA‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCAGGa                                                                                 >  2:191083/2‑149 (MQ=255)                                                                        tCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATg                                                                               <  7:343859/148‑1 (MQ=255)                                                                        tCCCAAAGCCGCAAACGGCTAAACGTGAACCTCCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGC‑GGATCGCCCGTAACCGTCTGCACACGCTGCATGACGC‑GATCCAGACGCaaa                                                                                             >  3:167486/1‑133 (MQ=255)                                                                        tCCAAAATCCGCATACGGCTAAACTTGAACCTGCGGAGGTGGAGTGATGAGCGACATTATCCGCCGCG‑TTCCAGGCGCCGATC‑GGATCGGCCGTAACCGTCTTCATCCGCTGCATGCCGC‑GATGCAGAGGCaag                                                                                             <  4:167486/134‑2 (MQ=255)                                                                          ccAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATgggg                                                                            <  8:348624/149‑1 (MQ=255)                                                                          ccAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATgggg                                                                            >  8:362436/1‑149 (MQ=255)                                                                            aaaGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGcc                                                                          >  3:141294/1‑149 (MQ=255)                                                                             aaGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGcc                                                                          >  2:233644/1‑148 (MQ=255)                                                                                ccGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAAc                                                                      <  6:179673/149‑1 (MQ=255)                                                                                   cAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGCCGCAACAGACCAGCTGGATGGGGCCCAACgg                                                                    >  6:101997/1‑148 (MQ=255)                                                                                          cTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATcc                                                            >  6:128671/1‑149 (MQ=255)                                                                                           tAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCg                                                           >  5:406187/1‑149 (MQ=255)                                                                                            aaaCGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGTGGGATCGCCCGTAACCCTCTGAACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCAACATCCg                                                           >  1:348517/1‑149 (MQ=255)                                                                                             aaCGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGc                                                          >  5:280441/1‑148 (MQ=255)                                                                                               cGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATAGCCCGTAACCGTCTGCACCCGCAGCATGCCGC‑GATTCAGACGCAACAGACCAGCTGGATTGGGCCCAAACGCAACATCCGCaa                                                        >  1:108013/1‑148 (MQ=255)                                                                                                gTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAg                                                       >  5:283057/1‑148 (MQ=255)                                                                                                 tGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGaa                                                     <  3:90934/149‑1 (MQ=255)                                                                                                   aaCCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAAcc                                                   >  3:85546/1‑149 (MQ=255)                                                                                                    aCCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAAccc                                                  <  6:283057/149‑1 (MQ=255)                                                                                                     ccTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCca                                                 >  3:212655/1‑149 (MQ=255)                                                                                                      cTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCca                                                 >  5:27505/1‑148 (MQ=255)                                                                                                        gCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACAt                                              >  5:18853/1‑149 (MQ=255)                                                                                                        gCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCAGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACAt                                              >  4:12185/1‑149 (MQ=255)                                                                                                           gAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGc                                            >  3:231766/1‑148 (MQ=255)                                                                                                              gTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGAtt                                        <  8:117664/149‑1 (MQ=255)                                                                                                                gCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGc                                      >  2:376774/1‑149 (MQ=255)                                                                                                                gCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGc                                      >  6:390977/1‑149 (MQ=255)                                                                                                                 cACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGcc                                     >  8:3925/1‑149 (MQ=255)                                                                                                                 cACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGcc                                     <  4:202964/149‑1 (MQ=255)                                                                                                                 cACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGcc                                     >  7:148369/1‑149 (MQ=255)                                                                                                                 cACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGc                                      <  7:336810/148‑1 (MQ=255)                                                                                                                  aCTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCg                                    <  4:141294/149‑1 (MQ=255)                                                                                                                    tgatgaGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTaa                                                                                                                                    <  6:270380/52‑1 (MQ=255)                                                                                                                    tgatgaGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTaa                                                                                                                                    >  5:270380/1‑52 (MQ=255)                                                                                                                    tgatgaGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCg                                    >  6:119488/1‑147 (MQ=255)                                                                                                                    tgatgaGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCg                                    >  2:434814/1‑147 (MQ=255)                                                                                                                    tgatgaGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCg                                    >  6:354986/1‑147 (MQ=255)                                                                                                                      atgaGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAgg                                >  5:14701/1‑149 (MQ=255)                                                                                                                      atgaGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAgg                                >  5:251033/1‑149 (MQ=255)                                                                                                                        gaGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCt                              >  6:381001/1‑149 (MQ=255)                                                                                                                          gCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCttt                            <  6:27505/149‑1 (MQ=255)                                                                                                                           cGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTg                           <  1:405902/149‑1 (MQ=255)                                                                                                                           cGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGAACAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCAACATGCGATTGCCGCAGGCTTTg                           >  2:299064/1‑149 (MQ=255)                                                                                                                            gACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGt                          <  5:119488/149‑1 (MQ=255)                                                                                                                              cATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTcg                        >  1:411532/1‑149 (MQ=255)                                                                                                                                ttATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTcggc                      <  3:83575/149‑1 (MQ=255)                                                                                                                                 tATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCatcatc                                                             <  6:228985/109‑1 (MQ=255)                                                                                                                                 tATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCatcatc                                                             >  5:228985/1‑109 (MQ=255)                                                                                                                                      gccgcG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACTTGCGATTGCCGCAGGCTTTGTcggcccggcc                >  7:373716/1‑149 (MQ=255)                                                                                                                                      gccgcG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTcggcccggcc                >  6:432916/1‑149 (MQ=255)                                                                                                                                      gccgcG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTcggcccggcc                >  7:208825/1‑149 (MQ=255)                                                                                                                                       gggcg‑ATCCACTCGGCGCGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCccggccg               >  6:323269/3‑149 (MQ=255)                                                                                                                                        cgcg‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATCGCCGCAGGCTTTGTCGGCccggccgg              <  1:233644/149‑1 (MQ=255)                                                                                                                                         gcg‑ATCCACTCGGCGCGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTg                                       >  4:68432/1‑123 (MQ=255)                                                                                                                                          cg‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCa            <  6:280441/149‑1 (MQ=255)                                                                                                                                           g‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCAt           >  7:53184/1‑149 (MQ=255)                                                                                                                                              ggatcgcgGCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCAAGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATc          >  1:304892/5‑148 (MQ=255)                                                                                                                                               ccACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCaa        >  8:248589/1‑149 (MQ=255)                                                                                                                                                     gcCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGCAt  <  4:85546/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                      GCCGCGCCCACAGATCAAGGACTTGGCCGAACTGGTGGCCGACGTGCTGATCGAGGAGGACGCATCCACTGTCCCAAAGCCGCAAACGGCTAAACGTGAACCTGCGGAGGTGCACTGATGAGCGACATTATCCGCCGCG‑ATCCACGCGCCGAGT‑GGATCGCCCGTAACCGTCTGCACCCGCTGCATGCCGC‑GATGCAGACGCAACAGACCAGCTGGATGGGGCCCAACGGCATCATCCGCAAGAACCCACATGCGATTGCCGCAGGCTTTGTCGGCCCGGCCGGCATCAAGCGCAT  >  NC_002947/374594‑374889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑