| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,945,368 | A→G | 24.5% | intergenic (+728/+70) | PP_5673 → / ← flhB | D‑alanine‑‑D‑alanine ligase/flagellin export apparatus substrate specificity protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,945,368 | 0 | A | G | 24.5% | 81.4 / 28.5 | 53 | intergenic (+728/+70) | PP_5673/flhB | D‑alanine‑‑D‑alanine ligase/flagellin export apparatus substrate specificity protein |
| Reads supporting (aligned to +/- strand): ref base A (30/10); new base G (5/8); total (35/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.20e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01 | |||||||||||
GCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGTGGCTCTGGGCGCTTGCCCTTGCCGGCGCG > NC_002947/4945220‑4945515 | ggtcggtcGTACATTTGTTTCGGGCCTGTTATTCCTGCGTGATTTACGCGCTACCGCCTTCGCTTAAACATCGATATTGCGCCGTACAAAAAAGGCGGTCGGGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGgagcga < 6:435507/147‑1 (MQ=255) aCATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTcgc > 2:361337/1‑149 (MQ=255) aTTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGa > 3:4421/1‑149 (MQ=255) ttGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATg > 1:14730/1‑149 (MQ=255) tttCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATgcgc > 3:282946/1‑149 (MQ=255) aGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCgcgggc > 8:69217/1‑149 (MQ=255) gTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCgcgggcg > 8:108457/1‑149 (MQ=255) aTTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGgcggcg > 5:14292/1‑149 (MQ=255) aTTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGgcggcg > 1:430151/1‑149 (MQ=255) gcgcgACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCaa > 7:120734/1‑149 (MQ=255) ccTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAAcc > 4:182310/1‑148 (MQ=255) cgcgTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTAt < 7:108457/147‑1 (MQ=255) gcgTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTAACAGGCAACGCAAACCTATCGc > 3:274036/1‑149 (MQ=255) gTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGcc > 2:293995/1‑148 (MQ=255) gTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGcc > 7:128245/1‑148 (MQ=255) cTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAgg < 6:406132/124‑1 (MQ=255) cTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAgg > 5:406132/1‑124 (MQ=255) cTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGaa > 2:33024/1‑145 (MQ=255) tCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTacccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacct < 7:247375‑M2/148‑67 (MQ=255) cgcCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTcg > 4:28732/1‑149 (MQ=255) gcCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCCACTGTcgc > 8:402383/1‑149 (MQ=255) ccGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTcgcg < 2:14730/149‑1 (MQ=255) tACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTacccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgcc < 1:214523‑M2/149‑82 (MQ=255) aaacaaGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAg > 6:375728/1‑149 (MQ=255) caaGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGtg > 4:119739/1‑148 (MQ=255) aaGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTcc > 7:128246/1‑149 (MQ=255) cGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGgcgc < 2:52748/139‑1 (MQ=255) cGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGgcgc > 1:52748/1‑139 (MQ=255) gcgcgcgcCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATc < 8:128245/149‑1 (MQ=255) gcgcgcgaCTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTacccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtccactcaaaattcc > 3:193720‑M2/1‑52 (MQ=255) gcgcgcCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCgg > 1:250049/1‑149 (MQ=255) tgcaggAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTacccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtccactcaaaattccaacgccgccccc < 3:67521‑M2/147‑110 (MQ=255) acaGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCt < 5:375728/149‑1 (MQ=255) aggaggGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCtt > 4:75722/1‑148 (MQ=255) aggGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGt < 7:69217/149‑1 (MQ=255) aCTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGTGGc > 8:101861/1‑148 (MQ=255) aCTGGCC‑GAAACAAACGTAGGAGCGGGTTTacccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtccactcaaaattccaacgccgccccctcaaaattcc > 2:369738‑M2/1‑30 (MQ=255) tggccgaaACAAACGTAGGAGCGGGTTTacccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtccactcaaaattccaacgccgccccctcaaaattccaa < 2:139395‑M2/146‑122 (MQ=255) gcacGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGTGGCTCTgg > 6:207461/4‑149 (MQ=255) gCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGTGGctct < 8:128246/147‑1 (MQ=255) gccgAAACAAACGTAGGAGCGGGTTTacccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtccactcaaaattccaacgccgccccctcaaaattccaacg < 5:84136‑M2/148‑124 (MQ=255) gccgAAACAAACGTAGGAGCGGGTTTacccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtccactcaaaattccaacgccgccccctcaaaatt > 6:278964‑M2/2‑26 (MQ=255) gccgAAACAAACGTAGGAGCGGGTTTacccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtccactcaaaattccaacgccgccccctcaaaatt < 5:278964‑M2/142‑118 (MQ=255) cccGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGTGGCTCTggg > 4:393292/1‑149 (MQ=255) ccGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGTGGCTCTGGgc > 4:375255/1‑149 (MQ=255) ccGAAACAAACGTAGGAGCGGGTTTacccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtccactcaaaattccaacgccgccccctcaaaattccaacgc < 7:158862‑M2/149‑125 (MQ=255) aaaCGTAGGAGCGGGTTTacccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtccactcaaaattccaacgccgccccctcaaaattccaac > 5:84008‑M2/1‑18 (MQ=255) aaaCGTAGGAGCGGGTTTacccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtccactcaaaattccaacgccgccccctcaaaattccaac < 6:84008‑M2/140‑123 (MQ=255) aaTGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGTGGCTCTGGGCGcttg > 7:107782/1‑146 (MQ=255) aaTGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGTGGCTCTGGGCGcttg < 8:107782/146‑1 (MQ=255) gTAGGAGCGAGCTTGCTCGCGATCCGCCGCCCGGCCGGCCCTCGATCTCACACGCAACGCAAACCTATCGCAGAACAGATCAACTCTCGCGGCGCAGGTCCCGCGCGAACCCCACCACGTCCTTCAGAGGCTCTCGGCGcttacccttg > 8:296022/1‑149 (MQ=21) tAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGTCGGGATCGGCAGGTCGTCCTTCAGTGGCTCTGGGCGcttgcccttgc > 8:115132/1‑149 (MQ=255) tAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGTGGCTCTGGGCGcttgcccttg < 4:274036/148‑1 (MQ=255) ggAGCGGGTTTacccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtccactcaaaattccaacgccgccccctcaaaattccaacgccgcctcccctcga > 1:208967‑M2/1‑11 (MQ=255) gagcgagcTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTGCGGCGGGATCGGCAGGTCGTCCTTCAGTGGCTCTGGGCGCTTGCCCTTGCCgg > 1:356084/1‑149 (MQ=255) gcgagcTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGTGGCTCTGGGCGCTTGCCCTTGCCGgcg > 4:154193/1‑149 (MQ=255) gagcTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGTGGCTCTGGGCGCTTGCCCTTGCCGGcgcg < 3:28732/149‑1 (MQ=255) | GCTCGCTCCTACATTTGTTTCGGGCCAGTTATTCCTGCGGGATTTACGCGCGACCGCCTTCGCGTACACCTCGATATTGCGCCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGAGGGACTGGCCCGAAACAAATGTAGGAGCGAGCTTGCTCGCGATGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCAACGCAAACCTATCGCCGAACAGATCAACTGTCGCGGCGCAGGTCCGGCGGGATCGGCAGGTCGTCCTTCAGTGGCTCTGGGCGCTTGCCCTTGCCGGCGCG > NC_002947/4945220‑4945515 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |