Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,950,972 T→G 20.3% intergenic (‑122/+35) fliL ← / ← fliK flagellar protein FliL/flagellar hook‑length control protein FliK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,950,9720TG20.3% 58.9 / 10.6 36intergenic (‑122/+35)fliL/fliKflagellar protein FliL/flagellar hook‑length control protein FliK
Reads supporting (aligned to +/- strand):  ref base T (12/16);  new base G (2/5);  total (14/22)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTCGCTCTTCGCCATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCA‑‑GGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCG‑GGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGGCGCGCCGCCACCC  >  NC_002947/4950835‑4951116
                                                                                                                                            |                                                                                                                                                
cTTCGCTCTTCGCCATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCA‑‑GGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCG‑GGCTCGCCCGCga                                                                                                                                         <  2:877/147‑1 (MQ=255)
   cGCTCTTTGCCATGCCAATAATCCGTGGGCCCTCGGGGGTTCAAATGATAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCATCGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCG‑GGCGAGCCCGCgaag                                                                                                                                       <  1:125085/148‑1 (MQ=255)
   cGCTCTTCGCCCTGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCA‑‑GGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCG‑GGCTCGCCCGCgaagaa                                                                                                                                     <  3:68761/148‑1 (MQ=255)
   cGCTCTTCGCCATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTTGCTTGAGCAAGTGTTATGCCAGATTTTGCA‑‑GGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCG‑GGCGAGCCCGCgaagaa                                                                                                                                     <  2:415751/148‑1 (MQ=255)
   cGCTCTTCGCCATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCA‑‑GGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCG‑GGCTCGCCCGCgaagaa                                                                                                                                     <  6:234739/148‑1 (MQ=255)
   cGCTCTTCGCCATGCCAATAATCCGGCGACCATCGGGGGTTCACAGGAGAATGGCTTGGCTTGAGCACGGGTTATGCCAGAGATTGCA‑‑GGCGAGGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCG‑GGCTCGCCCGCtaaga                                                                                                                                      <  5:303637/147‑1 (MQ=255)
    gCTCATCGCCATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCA‑‑GGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCG‑GGCCCGCCCGCGaagaag                                                                                                                                    <  7:368997/148‑1 (MQ=255)
        ttCGCCATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATTCCAGCTCTTGCA‑‑GGGGATGGATTCCAGCAGGTTTTGCGGTGCTAGCTTCTTCGCG‑GGCGAGCCCGCGAAGAagcca                                                                                                                                 <  1:354831/147‑1 (MQ=255)
                   aataatCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCA‑‑GGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCG‑GGCTCGCCCGCGAAGAAGCCCGCCCAGGCCAAcc                                                                                                                    >  7:262398/1‑148 (MQ=255)
                      aatCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCA‑‑GGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTGCGCG‑GGCGCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGtt                                                                                                                 <  1:408079/149‑1 (MQ=255)
                          cgtcgtCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCA‑‑GGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCG‑GGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAAc                                                                                                                     <  8:317165/141‑1 (MQ=255)
                          cgtcgtCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCA‑‑GGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCG‑GGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAAc                                                                                                                     >  7:317165/1‑141 (MQ=255)
                           gtcgtcCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCA‑‑GGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCG‑GGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAg                                                                                                            <  3:411941/149‑1 (MQ=255)
                                 cATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCA‑‑GGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCG‑GGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCgta                                                                                                       <  6:220913/148‑1 (MQ=255)
                                       ccgTTCTAAGGAGATTGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCA‑‑GGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCG‑GGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATc                                                                                                 <  3:231318/146‑1 (MQ=255)
                                             aaaTGAGTATGGCTTGGCTTGAGCATGTGTTAAGCCAGATTTTGCA‑‑GGGGATGGATTCCAGCAGGTTTTGCGGTGCTTGCTTCTTCGCGTCGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCg                                                                                            <  1:119811/148‑1 (MQ=255)
                                             aaaGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCA‑‑GGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCG‑GGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCa                                                                                          >  2:304440/1‑149 (MQ=255)
                                             aaaGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCA‑‑GGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCG‑GGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCa                                                                                          <  1:304440/149‑1 (MQ=255)
                                               aGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCA‑‑GGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCG‑GGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAgg                                                                                                           >  8:314680/1‑130 (MQ=255)
                                               aGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCA‑‑GGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCG‑GGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAgg                                                                                                           <  7:314680/130‑1 (MQ=255)
                                               aGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCA‑‑GGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCG‑GGCTCGCCCGCGAAGAAGCCACCCCAGGCCAACACTTAAAAGGCGTAGTAATCGagagagg                                                                                         >  4:231318/1‑142 (MQ=255)
                                                                   gCAAGTGTTATGCCAGATTTTGCA‑‑GGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCG‑GGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACctgct                                                                    >  7:333075/1‑149 (MQ=255)
                                                                              gCCAGATTTTGCA‑‑GGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCG‑GGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGc                                                         >  5:131418/1‑149 (MQ=255)
                                                                              gCCAGATTTTGCA‑‑GGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCG‑GGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGc                                                         >  1:329461/1‑149 (MQ=255)
                                                                                  gATTTTGCA‑‑GGGGATGGATTCCAGCTGGTTTTGCGGTGCTGGCTTCTTCGCG‑GGCGCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCggg                                                     <  6:128716/149‑1 (MQ=255)
                                                                                      ttGCA‑‑GGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCG‑GGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGgcgc                                                  <  8:333075/148‑1 (MQ=255)
                                                                                                            cagGTTTTGCGGTGCTGGCTTCTTCGCG‑GGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCAc                             >  7:199248/1‑149 (MQ=255)
                                                                                                                      ggTGCTGGCTTCTTCGCG‑GGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGc                   >  4:145965/1‑149 (MQ=255)
                                                                                                                       gTGCTGGCTTCTTCGCG‑GGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGcc                  <  4:411859/149‑1 (MQ=255)
                                                                                                                               ttcttcGCG‑GGCGAGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTAACGACCGCTTCGGCCCCACACTCCTCGGCCCGgagcgc          >  8:245453/1‑149 (MQ=255)
                                                                                                                                tcttcGCG‑GGCGAGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGGCGcgcc         >  3:250124/1‑149 (MQ=255)
                                                                                                                                 cttcGCG‑GGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGTCAACCACCTGCTGCTCCAAAGGGCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGgcgcgc          <  4:350717/147‑1 (MQ=255)
                                                                                                                                 cttcGCG‑GGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGgcgcgc          <  5:272322/147‑1 (MQ=255)
                                                                                                                                   tcGCG‑GGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGGCGcgccgc       >  4:294548/1‑148 (MQ=255)
                                                                                                                                   tcGCG‑GGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGGCGCgccgcc      >  6:157544/1‑149 (MQ=255)
                                                                                                                                       g‑ggCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGGCGCGCCGCCAccc  <  3:145965/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                
CTTCGCTCTTCGCCATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCA‑‑GGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCG‑GGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGGCGCGCCGCCACCC  >  NC_002947/4950835‑4951116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑