Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,968,140	A→G	5.9%	intergenic (‑36/+39)	PP_4377 ← / ← fliC	flagellin FlaG/flagellin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,968,140	0	A	G	5.9%	249.9 / 4.5	85	intergenic (‑36/+39)	PP_4377/fliC	flagellin FlaG/flagellin
Reads supporting (aligned to +/- strand):  ref base A (44/36);  new base G (2/3);  total (46/39)
Fisher's exact test for biased strand distribution p-value = 6.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.80e-01

TTGGTCGGCACGTCTGGTAGCTCAGCATTCGATCTGCTCGATGGCTGTGCACTCCTGGCTTCTTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCTTGCTGCAGGGTTTGCTGCTTGCTCAGCTCCGCAGTTTCGG  >  NC_002947/4967992‑4968285                                                                                                                                                     |                                                                                                                                                  ttGGTCGGCACGTCTGGTAGCTCAGCATTCGATCTGCTCGATGGCTGTGCACTCCTGGCTTCTTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTa                                                                                                                                                   >  1:388717/1‑149 (MQ=255)       ggCACGTCTGGTAGCTCAGCATTCGATCTGCTCGATGGCTGTGCACTCCTGGCTTCTTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGAACGTTGc                                                                                                                                             >  6:219350/1‑149 (MQ=255)       ggCAAGTCTGGTAGCTCAGCATTCGATCTTCTCGATGTCTTTGCACTCCTGTCTTCTTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGc                                                                                                                                             <  5:311059/149‑1 (MQ=255)         cACGTCTGGTAGCTCAGCATTCGATCTGCTCGATGGCTGTGCACTCCTGGCTTCTTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCg                                                                                                                                           <  5:50804/149‑1 (MQ=255)           cGTCTGGTAGCTCAGCATTCGATCTGCTCGATGGCTGTGCACTCCAGGCTTCTTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCa                                                                                                                                         <  1:194988/149‑1 (MQ=255)                 gTAGCTCAGCATTCGATCTGCTCGATGGCTGTGCACTCCTGGCTTCTTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCttt                                                                                                                                   <  7:117085/149‑1 (MQ=255)                 gTAGCTCAGCATTCGATCTGCTCGATGGCTGTGCACTCCTGGCTTCTTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCtt                                                                                                                                    <  2:388717/148‑1 (MQ=255)                  tAGCTCAGCATTCGATCTGCTCGATGGCTGTGCACTCCTGGCTTCTTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTc                                                                                                                                  >  3:78977/1‑149 (MQ=255)                    gCTCAGCATTCGATCTGCTCGATGGCTGTGCACTCCTGGCTTCTTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGGAAGGGTACGTTGCCGCACCCtt                                                                                                                                    >  1:372760/1‑145 (MQ=255)                    gCTCAGCATTCGATCTGCTCGATGGCTGTGCACTCCTGGCTTCTTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTGCGGCAACGTACCCTTTcg                                                                                                                                 <  3:367207/148‑1 (MQ=255)                    gCTCAGCATTCGATCTGCTCGATGGCTGTGCACTCCTGGCTTCTTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTcgc                                                                                                                                >  5:346414/1‑149 (MQ=255)                      tCAGCATTCGATCTGCTCGATGGCTGTGCACTCCTGGCTTCTTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGa                                                                                                                              >  7:362222/1‑149 (MQ=255)                        cgCATTCGATCTGCGCGTTGGCTGTGCACTCCTGGCTTCGTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAACACGAAACGCTACGTTGCCGCACCCTTTCGCGa                                                                                                                              <  6:130606/146‑1 (MQ=255)                        aGCATTCGATCTGCTCGATGGCTGTGCACTCCTGGCTTCTTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGAt                                                                                                                             >  8:11401/1‑148 (MQ=255)                         gCATTCGATCTGCTCGATGGCTGTGCACTCCTGGCTTCTTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATtc                                                                                                                           <  7:11401/149‑1 (MQ=255)                           aTTCTATATGCTCGATGGCTGTGCACTCCTGGATTCTTGTGTTGCGTGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCATAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATtcat                                                                                                                         <  2:14593/149‑1 (MQ=255)                                     cTCGATGGCTGTGCACTCCTGGCTTCTTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTGCGGCAACGTACCCTTTcgt                                                                                                                                <  7:69727/132‑2 (MQ=255)                                     cTCGATGGCTGTGCACTCCTGGCTTCTTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTGCGGCAACGTACCCTTTcgt                                                                                                                                >  8:69727/1‑131 (MQ=255)                                     cTCGATGGCTGTGCACTCCTGGCTTCTTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATcaca                                                                                                                     <  7:30914/143‑1 (MQ=255)                                     cTCGATGGCTGTGCACTCCTGGCTTCTTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATc                                                                                                                >  4:271522/1‑148 (MQ=255)                                       cGATGGCTGTGCACTCCTGGCTTCTTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGaa                                                                                                             >  4:285971/1‑149 (MQ=255)                                          tGGCTGTGCACTCCTGGCTTCTTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTa                                                                                                          <  4:78977/149‑1 (MQ=255)                                          tGGCTGTGCACTCCTGGCTTCTTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTa                                                                                                          <  3:96444/149‑1 (MQ=255)                                                 gCACTCCTGGCTTCTTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGag                                                                                                    >  6:247543/1‑148 (MQ=255)                                                 gCACTCCTGGCTTCTTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGAGATTCATCACAGAATCGAATTAGCCGag                                                                                                    >  6:241526/1‑148 (MQ=255)                                                   aCTCCTGGCTTCTTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGagcag                                                                                                 >  3:261531/1‑149 (MQ=255)                                                   aCTCCTGGCTTCTTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGagcag                                                                                                 >  6:229101/1‑149 (MQ=255)                                                        tGGCTTCTTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCa                                                                                            >  2:44526/1‑149 (MQ=255)                                                               ttGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCg                                                                                      >  5:10645/1‑148 (MQ=255)                                                                 gAGTGGCTGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGTGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGaa                                                                                    <  2:311048/148‑1 (MQ=255)                                                                 gAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGaa                                                                                    <  3:285971/148‑1 (MQ=255)                                                                 gAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGaa                                                                                    <  5:229101/148‑1 (MQ=255)                                                                  aGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGa                                                                                  <  4:261531/149‑1 (MQ=255)                                                                   gTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTTCCGAAGAt                                                                                 <  8:362222/149‑1 (MQ=255)                                                                   gTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGAt                                                                                 <  6:346414/149‑1 (MQ=255)                                                                      gcgggcgCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGCAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGc                                                                              <  2:372760/149‑1 (MQ=255)                                                                             cAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGt                                                                       >  7:317698/1‑149 (MQ=255)                                                                                agagaACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATTGCATCTGGTTCg                                                                    >  3:51198/1‑149 (MQ=255)                                                                                  agaACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGtt                                                                      >  5:345717/1‑145 (MQ=255)                                                                                  agaACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCATCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGtt                                                                      <  6:345717/145‑1 (MQ=255)                                                                                    aaCAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTg                                                                >  7:187797/1‑149 (MQ=255)                                                                                         agTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGa                                                             >  8:81618/1‑147 (MQ=255)                                                                                          gTTCAGCTTGACACGCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAg                                                          >  7:367731/1‑149 (MQ=255)                                                                                           ttCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCCAATTAGCAGAGCAGCTTCAGAACCGAAGATCGCAGATTGTTCGCATGCGACac                                                          >  5:292078/1‑147 (MQ=255)                                                                                                cTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGc                                                    <  2:219395/149‑1 (MQ=255)                                                                                                  tGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCgg                                                  >  4:135898/1‑149 (MQ=255)                                                                                                   gACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGGAGCTGGTTCGCCTGCGACAGGATAGCGGt                                                 >  5:385224/1‑149 (MQ=255)                                                                                                   gACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCgg                                                  <  3:135898/148‑1 (MQ=255)                                                                                                   gACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCgg                                                  <  3:271522/148‑1 (MQ=255)                                                                                                    acacTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGt                                                 <  8:317698/148‑1 (MQ=255)                                                                                                      acTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTgg                                               >  2:402389/1‑148 (MQ=255)                                                                                                        tCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAg                                            >  1:167676/1‑149 (MQ=255)                                                                                                        tCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAg                                            >  5:306936/1‑149 (MQ=255)                                                                                                          atatCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCg                                                   >  8:15601/1‑140 (MQ=255)                                                                                                          atatCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCg                                                   <  7:15601/140‑1 (MQ=255)                                                                                                          atatCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCt                                          >  5:156096/1‑149 (MQ=255)                                                                                                              ccATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCTtgct                                      >  5:408638/1‑149 (MQ=255)                                                                                                                atgatgCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCTtgctg                                     >  2:27170/1‑148 (MQ=255)                                                                                                                    tgCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCTTGCTGCAgg                                 <  6:10645/148‑1 (MQ=255)                                                                                                                    tgCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCAGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCTTGCTGCAgg                                 <  6:274841/148‑1 (MQ=255)                                                                                                                     gCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCTTGCTGCAGGGt                               >  7:351793/1‑149 (MQ=255)                                                                                                                       ttGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCTTGCTGCAGGGttt                             >  6:17650/1‑149 (MQ=255)                                                                                                                       ttGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTATCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCTTGCTGCAGGGttt                             >  1:95901/1‑149 (MQ=255)                                                                                                                          ccTCGTAGAATCAGAAAACGAAAGGGGACGTTGCCGCACCCTTTCGCGCTTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGGCTGCGTCAGGAGAGCGGTGGACGCTTGCTGCCGGGTTtgct                          >  2:266786/1‑149 (MQ=255)                                                                                                                            tCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCTTGCTGCAGGGTTtgctgc                        >  6:10375/1‑149 (MQ=255)                                                                                                                              gTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCTTGCTGCAGGGTTtgctgc                        <  1:402389/147‑1 (MQ=255)                                                                                                                              gTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCTTGCTGCAGGGTTtgctgc                        <  1:44526/147‑1 (MQ=255)                                                                                                                              gTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCTTGCTGCAGGGTTtgctgc                        <  5:247543/147‑1 (MQ=255)                                                                                                                              gTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCTTGCTGCAGGGTTtgctgc                        <  5:241526/147‑1 (MQ=255)                                                                                                                              gTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCTTGCTGCAGGGTTtgctgc                        <  6:385224/147‑1 (MQ=255)                                                                                                                              gTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCTTGCTGCAGGGTTTGCtgctt                      <  2:357533/149‑1 (MQ=255)                                                                                                                               tAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGaa                                             <  4:306040/125‑1 (MQ=255)                                                                                                                               tAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGaa                                             >  3:306040/1‑125 (MQ=255)                                                                                                                               tAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCTTGCTGCAGGGTTTGCtgcttg                     >  5:256617/1‑149 (MQ=255)                                                                                                                                 taaTCATAAAACGAAATGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACATGATAGCGGTGGAAGCTTGCTGCAGGGTTTGCtgcttgct                   <  8:367731/148‑1 (MQ=255)                                                                                                                                  aaTCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCTTGCTGCAGGGt                               <  8:117246/136‑1 (MQ=255)                                                                                                                                  aaTCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCTTGCTGCAGGGt                               >  7:117246/1‑136 (MQ=255)                                                                                                                                    tCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCTTGCTGCAGGGTTTGCTGCTTGCTCAg                >  2:10688/1‑149 (MQ=255)                                                                                                                                       gAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCTTGCTGCAGGGTTTGCTGCTTGCTCAGCTc             >  3:125263/1‑149 (MQ=255)                                                                                                                                       aatcgcGAAAGGGTGCGGCAACGTACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCTTGCTGCAGGGTTtgctg                         <  5:219973/132‑1 (MQ=38)                                                                                                                                       aatcgcGAAAGGGTGCGGCAACGTACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCTTGCTGCAGGGTTtgctg                         >  6:219973/6‑137 (MQ=38)                                                                                                                                          aaCGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCTTGCTGCAGTGTTTGCTGCTTGCTCAGCTcag           >  5:116373/1‑146 (MQ=255)                                                                                                                                            cGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCTTGCTGCAGGGTTTGCTGCTTGCTCAGCTCCGCAg        >  4:297872/1‑149 (MQ=255)                                                                                                                                            cGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCTTGCTGCAGGGTTTGCTGCTTGCTCAGCTACGCAg        >  4:292261/1‑149 (MQ=255)                                                                                                                                                aGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCTTGCTGCAGGGTTTGCTGCTTGCTCAGCTCCGCAGTTTc    <  6:256617/149‑1 (MQ=255)                                                                                                                                                  ggTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCTTGCTGCAGGGTTTGCTGCTTGCTCAGCTCCGCAGTTTCgg  >  1:50378/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                  TTGGTCGGCACGTCTGGTAGCTCAGCATTCGATCTGCTCGATGGCTGTGCACTCCTGGCTTCTTGAGTGGCGGGCGCAGAGAGAACAGAGTTCAGCTTGACACTCATATCCATGATGCTTGCCTCGTAGAATCAGAAAACGAAAGGGTACGTTGCCGCACCCTTTCGCGATTCATCACAGAATCGAATTAGCCGAGCAGCTTCAGTACCGAAGATGGCAGCTGGTTCGCCTGCGACAGGATAGCGGTGGAAGCTTGCTGCAGGGTTTGCTGCTTGCTCAGCTCCGCAGTTTCGG  >  NC_002947/4967992‑4968285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑