Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,032,756	C→G	7.1%	P92R (CCT→CGT) ‡	PP_4435 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,032,756	0	C	G	7.1%	267.6 / 6.6	83	P92R (CCT→CGT) ‡	PP_4435	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (42/35);  new base G (4/2);  total (46/37)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTGGCGAACATTGACAGGCTTCTTGAGGAGGCCGGTAGTCACAAGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTAAAAGCGCACCCGGCAGGCGCCTATCCAGCCTGGTAACAGGTCAGAGGCGCTCGCCGGGC  >  NC_002947/5032610‑5032900                                                                                                                                                    |                                                                                                                                                 ccTGGCGAACATTGACAGGCTTCTTGAGGAGGCCGGTAGTCACAAGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTg                                                                                                                                                >  4:354724/1‑149 (MQ=255) ccTGGCGAACATTGACAGGCTTCTTGAGGAGGCCGGTAGTCACAAGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTg                                                                                                                                                >  7:331656/1‑149 (MQ=255)  cTGGCGAACATTGACAGGCTTCTTGAGGAGGCCGGTAGTCACAAGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTgc                                                                                                                                               >  7:391208/1‑149 (MQ=255)      cGAACATTGACAGGCTTCTTGAGGAGGCCGGTAGTCACAAGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTgcgc                                                                                                                                             >  8:369680/1‑147 (MQ=255)      cGAACATTGACAGGCTTCTTGAGGAGGCCGGTAGTCACAAGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGa                                                                                                                                           <  3:354724/149‑1 (MQ=255)       gAACATTGACAGGCTTCTTGAGGAGGCCGGTAGTCACAAGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAg                                                                                                                                          >  3:231884/1‑149 (MQ=255)       gAACATTGACAGGCTTCTTGAGGAGGACGGTAGTCACAAGTAGAAAATCCTCACAGTGACTATCTATCTGAACGACATCAACACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCTG‑AAGGTCAGTCTCCTGCGCGAg                                                                                                                                          >  1:417113/1‑149 (MQ=255)        aaCATTGACAGGCTTCTTGAGTAGGCCGGTAGTCACAAGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGc                                                                                                                                         <  6:141522/149‑1 (MQ=255)        aaCATTGACAGGCTTCTTGAGGAGGCCGGTAGTCACAAGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGc                                                                                                                                         <  8:359498/149‑1 (MQ=255)            ttGACAGGCTTCTTGAGGAGGCCGGTAGTCACAAGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCGCGCAGGAGCCtga                                                                                                                                     <  8:358780/149‑2 (MQ=255)              gACAGGCTTCTTGAGGAGGCCGGTAGTCACAAGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTgtgt                                                                                                                                   >  5:28624/1‑149 (MQ=255)                cAGGCTTCTTGAGGAGGCCGGTAGTCACAAGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTg                                                                                                                                 <  1:79101/149‑1 (MQ=255)                 atggttcttGAGGAGGCCGGTAGTCACAAGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGa                                                                                                                                <  5:4582/145‑1 (MQ=255)                 aGGCTTCTTGAGGAGGCCGGTAGTCACAAGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCGCGCAGGAGCCtgac                                                                                                                                    >  2:248370/1‑143 (MQ=255)                 aGGCTTCTTGAGGAGGCCGGTAGTCACAAGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCGCGCAGGAGCCtgac                                                                                                                                    <  1:248370/145‑3 (MQ=255)                 aGGCTTCTTGAGGAGGCCGGTAGTCACAAGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCtgt                                                                                                                                     <  5:7242/144‑1 (MQ=255)                 aGGCTTCTTGAGGAGGCCGGTAGTCACAAGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCtgt                                                                                                                                     >  6:7242/1‑144 (MQ=255)                   gCTTCTTGAGGAGGCCGGTAGTCACAAGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAgg                                                                                                                              >  8:261772/1‑149 (MQ=255)                        ttGAGGAGGCCGGTAGTCACAAGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAggcggc                                                                                                                          >  8:113887/1‑148 (MQ=255)                                gCCGGTAGTCACAAGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTcaa                                                                                                                 >  2:73880/1‑149 (MQ=255)                                gCCGGTAGTCACAAGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTc                                                                                                                   >  3:204425/1‑147 (MQ=255)                                 ccGGTAGTCACAAGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTcaag                                                                                                                <  2:186105/149‑1 (MQ=255)                                     gaGTCACAAGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTcaagccaa                                                                                                            <  4:324677/148‑1 (MQ=255)                                       gTCACAAGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCTACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTcaagccaagc                                                                                                          <  1:196323/149‑1 (MQ=255)                                        tCACAAGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCaagccaagcc                                                                                                         <  3:15169/149‑1 (MQ=255)                                           caAGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGc                                                                                                      >  4:151135/1‑149 (MQ=255)                                           caAGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGc                                                                                                      >  5:285472/1‑149 (MQ=255)                                            aactCGAAAATCCTCACACGGACTATCTATCTGAACGACATCACCACCGTCTTCGGGGCCATGAACGAGGTTTGGGAACCGTGGGGCGCGGTAGGGTCAGGCTCCTGCGCGAGCCTGTGTTg                                                                                                                                 <  1:3918/119‑1 (MQ=255)                                            aaGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGAGTTCGCTGCCATGAACGAGGTTTGGGATGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCt                                                                                                     >  6:72397/1‑149 (MQ=255)                                            aaGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTg                                                                                                                                 >  2:3918/1‑121 (MQ=255)                                            aaGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTcaa                                                                                                                 >  3:247259/1‑137 (MQ=255)                                            aaGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTcaa                                                                                                                 <  4:247259/137‑1 (MQ=255)                                             aGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTc                                                                                                    <  6:196816/149‑1 (MQ=255)                                                 gAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGtt                                                                                                 >  4:237704/1‑148 (MQ=255)                                                 gAAAATCCTCACAGTGACTATCGATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGtt                                                                                                 >  6:70928/1‑148 (MQ=255)                                                  aaaaTCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTga                                                                                               <  7:261772/149‑1 (MQ=255)                                                   aaaTCCTCACATTTAATATCTATCTGAACGACATCACCACCGACTTCGCTTCCATGAACGAGGTTTGGGAAGAGTTGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTgag                                                                                              <  5:263982/149‑1 (MQ=255)                                                   aaaTCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTgag                                                                                              <  1:56517/149‑1 (MQ=255)                                                    aaTCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTgaga                                                                                             <  1:73880/149‑1 (MQ=255)                                                         tCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTAt                                                                                                                       >  7:210123/1‑118 (MQ=255)                                                         tCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTAt                                                                                                                       <  8:210123/118‑1 (MQ=255)                                                           acaGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTcc                                                                                        <  6:285472/147‑1 (MQ=255)                                                           acaGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCg                                                                                       >  3:316514/1‑148 (MQ=255)                                                             aGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCgtag                                                                                    <  8:331656/149‑1 (MQ=255)                                                                     tctatctGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTagcagc                                                                             >  3:99346/1‑148 (MQ=255)                                                                        atctGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATc                                                                         >  5:177887/1‑149 (MQ=255)                                                                         tctGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCg                                                                        >  1:15084/1‑149 (MQ=255)                                                                         tctGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCg                                                                        >  2:281231/1‑149 (MQ=255)                                                                            gAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGtt                                                                                                 >  8:24668/1‑121 (MQ=255)                                                                            gAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGtt                                                                                                 <  7:24668/121‑1 (MQ=255)                                                                             aaCGACATCACCCCCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCt                                                                     <  4:254442/148‑1 (MQ=255)                                                                             aaCGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGc                                                                       <  6:177887/146‑1 (MQ=255)                                                                             aaCGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCt                                                                     >  6:183121/1‑148 (MQ=255)                                                                                      accaccGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTaaa                                                           <  7:369680/149‑1 (MQ=255)                                                                                      accaccGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTaa                                                            >  2:316459/1‑148 (MQ=255)                                                                                       ccaccGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTaaaa                                                          <  7:113887/149‑1 (MQ=255)                                                                                        caccGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTAAAAg                                                         >  2:403250/1‑149 (MQ=255)                                                                                         accGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTAAAAgc                                                        <  8:391208/149‑1 (MQ=255)                                                                                                      ccATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTAAAAGCGCACCCgg                                                >  5:424020/1‑144 (MQ=255)                                                                                                      ccATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTAAAAGCGCACCCgg                                                <  6:424020/144‑1 (MQ=255)                                                                                                        aTGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTAAAAGCGCACCCGGCAGgcgc                                         >  2:89892/1‑149 (MQ=255)                                                                                                        aTGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTAAAAGCGCACCCGGCAGgcgc                                         >  4:29062/1‑149 (MQ=255)                                                                                                        aTGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTAAAAGCGCACCCGGCAGgcgc                                         >  7:207638/1‑149 (MQ=255)                                                                                                         tGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTAAAAGCGCACCCGGCAGGCGcc                                        >  6:274037/1‑149 (MQ=255)                                                                                                              gAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTAAAAGCGCACCCGGCAGGCGCCTa                                      <  8:244501/146‑1 (MQ=255)                                                                                                              gAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTAAAAGCGCACCCGGCAGGCGCCTa                                      >  7:244501/1‑146 (MQ=255)                                                                                                              gAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTAAAAGCGCACCCGGCAGGCGCCTATc                                    >  3:320753/1‑148 (MQ=255)                                                                                                               aGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTAAAAGCGCACCCGGCAGGCGCCTATCCa                                  <  6:28624/149‑1 (MQ=255)                                                                                                               aGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTAAAAGCGCACCCGGCAGGCGCCTATCCa                                  <  4:231884/149‑1 (MQ=255)                                                                                                                 gTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTAAAAGCGCACCCGGCAGGCGCCTATCCAg                                 <  4:204425/148‑1 (MQ=255)                                                                                                                    tGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTAAAAGCGCACCCGGCAGGCGCCTATCCAGCCTg                             >  1:51882/1‑149 (MQ=255)                                                                                                                      ggAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTAAAAGCGCACCCGGCAGGCGCCTATCCAGCCTgg                            >  1:216801/1‑148 (MQ=255)                                                                                                                          gagTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTAAAAGCGCACCCGGCAGGCGCCTATCCAGCCTGGTAACa                       <  3:163446/149‑1 (MQ=255)                                                                                                                               ggTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTAAAAGCGCACCCGGCAGGCGCCTATCCAGCCTGGTAACAGGt                    >  6:66997/1‑147 (MQ=255)                                                                                                                                 tCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTAAAAGCGCACCCGGCAGGCGCCTATCCAGCCTGGTAACAGGTCaga                <  1:281231/149‑1 (MQ=255)                                                                                                                                           cacAGGCTCGCGCAGGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTAAAAGCGCACCCGGCAGGCGCCTATCCAGCCTGGTAACAGGTCAGAGGCGCTCGc       >  1:414246/3‑149 (MQ=255)                                                                                                                                            tCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGGAATCGACTGACGCTTAAAAATCCACACTGCAGGCGCCTATCCAGCCTGGTAACAGGTCAGAGGCGCTCGcc      >  1:142550/1‑149 (MQ=255)                                                                                                                                            tCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTAAAAGCGCACCCGGCAGGCGCCTATCCAGCCTGGTAACAGGTCAGAGGCGCTCGcc      >  6:98611/1‑149 (MQ=255)                                                                                                                                            acAGGCTCGCGCAGGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTAAAAGCGCACCCGGCAGGCGCCTATCCAGCCTGGTAACAGGTCAGAGGCGCTCGcc      >  2:351053/2‑149 (MQ=255)                                                                                                                                             cAGGCTCGCGCAGGAGCCTGTGATGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGACCGTAGTAGCAGCAATCGCCTGACGCTTAAAAGAGCACCCTGCAGGCGCCTATCCAGCCTGGTAACAGGTCACAGGCGCTCGCCg     >  7:387798/1‑149 (MQ=255)                                                                                                                                                gCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTAAAAGCGCACCCGGCAGGCGCCTATCCAGCCTg                             <  2:222591/122‑1 (MQ=255)                                                                                                                                                gCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTAAAAGCGCACCCGGCAGGCGCCTATCCAGCCTg                             >  1:222591/1‑122 (MQ=255)                                                                                                                                                gCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTAAAAGCGCACCCGGCAGGCGCCTATCCAGCCTGGTAACAGGTCAGAGGCGCTCGCCGGGc  <  4:316514/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                 CCTGGCGAACATTGACAGGCTTCTTGAGGAGGCCGGTAGTCACAAGTCGAAAATCCTCACAGTGACTATCTATCTGAACGACATCACCACCGACTTCGCTGCCATGAACGAGGTTTGGGAAGAGTGGGTCGCGG‑AAGGTCAGGCTCCTGCGCGAGCCTGTGTTGAGGCGGCTATGTTCAAGCCAAGCCTGCTCGTTGAGATGTCCGTAGTAGCAGCAATCGCCTGACGCTTAAAAGCGCACCCGGCAGGCGCCTATCCAGCCTGGTAACAGGTCAGAGGCGCTCGCCGGGC  >  NC_002947/5032610‑5032900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑