BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,098,182	T→G	5.9%	I404L (ATT→CTT)	acsA‑I ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,098,182	0	T	G	5.9%	320.5 / 9.5	101	I404L (ATT→CTT)	acsA‑I	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base T (48/47); new base G (2/4); total (50/51)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.75e-01

Only 100 of 102 total aligned reads displayed.

ACGATCGGGCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCC  >  NC_002947/5098034‑5098329

acgaTCGGGCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAAt                                                                                                                                                     >  2:9998/1‑149 (MQ=255)

  gaTCGGGCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCTTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATgg                                                                                                                                                   >  4:418314/1‑149 (MQ=255)

  gaTCGGGCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATgg                                                                                                                                                   <  6:279356/149‑1 (MQ=255)

  gaTCGGGCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATgg                                                                                                                                                   <  7:400941/149‑1 (MQ=255)

   aTCGGGCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATgg                                                                                                                                                   >  2:315972/1‑148 (MQ=255)

    tCGGGCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCa                                                                                                                                                 <  1:9998/149‑1 (MQ=255)

    tCGGGCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCATTCGCGCCATGATGGC‑CGAAGGGCa                                                                                                                                                 <  3:119081/149‑1 (MQ=255)

      gggCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACTCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCATTCGCGCCATGATGGC‑CGAAGGGCAgg                                                                                                                                               <  8:271932/149‑2 (MQ=255)

      gggCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCATTCGCGCCATGATGGC‑CGAAGGGCAgg                                                                                                                                               <  5:266388/149‑2 (MQ=255)

               tCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCtgt                                                                                                                                      >  7:284342/1‑149 (MQ=255)

                    gCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTa                                                                                                                                   <  5:55746/147‑1 (MQ=255)

                      cGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTaga                                                                                                                                 >  8:162684/1‑147 (MQ=255)

                      cGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTaga                                                                                                                                 <  7:162684/147‑1 (MQ=255)

                       gACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGa                                                                                                                              >  4:203843/1‑149 (MQ=255)

                        aCGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGAt                                                                                                                             >  5:298832/1‑149 (MQ=255)

                         cGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATg                                                                                                                            <  5:37148/149‑1 (MQ=255)

                                gtagtaCCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGAcc                                                                                                                      <  3:395826/148‑1 (MQ=255)

                                gtagtaCCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCt                                                                                                                     >  4:102936/1‑149 (MQ=255)

                                  agtaCCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTtg                                                                                                                   >  7:371414/1‑149 (MQ=255)

                                   gtaCCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTtgt                                                                                                                  <  6:208636/149‑1 (MQ=255)

                                    taCCAGTTCCAGGCCTCAGGGTTTATCGGCTCACCCACGGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCACCAGCTGGCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTtgt                                                                                                                  <  5:359110/148‑1 (MQ=255)

                                    taCCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTtgtg                                                                                                                 <  5:403758/149‑1 (MQ=255)

                                       cAGTTCCAGGCGTCTCGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCtt                                                                                                              <  4:321488/149‑1 (MQ=255)

                                        aGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACAGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTg                                                                                                             >  5:149796/1‑149 (MQ=255)

                                         gTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATgg                                                                                                                                                   <  6:56306/110‑1 (MQ=255)

                                         gTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATgg                                                                                                                                                   >  5:56306/1‑110 (MQ=255)

                                            ccAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTtgtg                                                                                                                 >  1:86603/1‑141 (MQ=255)

                                            ccAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTtgtg                                                                                                                 <  2:86603/141‑1 (MQ=255)

                                             cAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTcgac                                                                                                        >  8:149468/1‑149 (MQ=255)

                                               ggCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCtt                                                                                                              <  7:388325/141‑1 (MQ=255)

                                               ggCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCtt                                                                                                              >  8:388325/1‑141 (MQ=255)

                                                gCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGAt                                                                                                     >  2:153465/1‑149 (MQ=255)

                                                 ccTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATc                                                                                                    <  5:6923/149‑1 (MQ=255)

                                                  cTCAGGGTAGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTAG‑GCCATCATGGCGCGAATCGCAGTTGGCGCTGAGTAGAGGATGTTGACCTAGTGCTTGTCGACGATc                                                                                                    >  6:173116/1‑148 (MQ=255)

                                                         ttGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCtt                                                                                                                    <  7:124110/125‑1 (MQ=255)

                                                         ttGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCtt                                                                                                                    >  8:124110/1‑125 (MQ=255)

                                                            aTCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCtt                                                                                                                    >  7:158625/1‑122 (MQ=255)

                                                            aTCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCtt                                                                                                                    <  8:158625/122‑1 (MQ=255)

                                                            aTCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATAcgc                                                                                          <  3:135616/148‑1 (MQ=255)

                                                               ggCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGt                                                                                      <  7:286346/149‑1 (MQ=255)

                                                                gCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCGTGTCGACGATCTTCGATACGCGGGt                                                                                      >  6:123608/1‑148 (MQ=255)

                                                                    acccaccGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGTGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAAt                                                                                   <  1:315972/147‑1 (MQ=255)

                                                                    acccaccGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCTGGTAATg                                                                                  <  5:173116/148‑1 (MQ=255)

                                                                      ccaccGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTg                                                                                                                                             >  7:228224/1‑87 (MQ=255)

                                                                      ccaccGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTg                                                                                                                                             <  8:228224/87‑1 (MQ=255)

                                                                        accGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCgg                                                                             <  2:45249/149‑1 (MQ=255)

                                                                         ccGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCggg                                                                            <  2:9597/149‑1 (MQ=255)

                                                                                cagcagACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTgg                                                                     <  6:149796/149‑1 (MQ=255)

                                                                                cagcagACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTgg                                                                     <  6:298832/149‑1 (MQ=255)

                                                                                 agcagACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTggg                                                                    >  5:331041/1‑149 (MQ=255)

                                                                                   cagACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCa                                                                  <  3:203843/149‑1 (MQ=255)

                                                                                    agACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCAc                                                                 <  3:102936/149‑1 (MQ=255)

                                                                                      aCGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCggg                                                                            >  4:72951/1‑136 (MQ=255)

                                                                                      aCGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCggg                                                                            <  3:72951/136‑1 (MQ=255)

                                                                                      aCGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGc                                                               >  7:61862/1‑149 (MQ=255)

                                                                                      aCGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGc                                                               >  7:64725/1‑149 (MQ=255)

                                                                                        gCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTaa                                                                                    >  7:311825/1‑126 (MQ=255)

                                                                                        gCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTaa                                                                                    <  8:311825/126‑1 (MQ=255)

                                                                                        gCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCt                                                             >  3:421178/1‑149 (MQ=255)

                                                                                                gAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAAc                                                       <  7:149468/147‑1 (MQ=255)

                                                                                                gAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAg                                                     >  4:104839/1‑149 (MQ=255)

                                                                                                 aaCCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCgg                                                                                        >  4:321484/1‑113 (MQ=255)

                                                                                                 aaCCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAg                                                     >  2:303433/1‑148 (MQ=255)

                                                                                                  aCCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGc                                                    >  4:203581/1‑148 (MQ=255)

                                                                                                         gcgcCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAggtggt                                            <  8:64725/149‑1 (MQ=255)

                                                                                                          cgcCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGgtggtg                                           <  3:203581/149‑1 (MQ=255)

                                                                                                           gcCTTCAACATCTTCCTGCCCTTCG‑GCCATCATGGGGCGAATTGCAGTTGGCTCTGTGTAGATGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGgtggtg                                           <  3:116074/148‑1 (MQ=255)

                                                                                                           gcCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGtggtgg                                          <  6:331041/149‑1 (MQ=255)

                                                                                                             cTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGgcg                                        >  3:129366/1‑149 (MQ=255)

                                                                                                               tCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAggt                                               <  5:350200/140‑1 (MQ=255)

                                                                                                               tCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAggt                                               >  6:350200/1‑140 (MQ=255)

                                                                                                               tCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGcc                                      >  8:361052/1‑149 (MQ=255)

                                                                                                                  aCAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGtt                                   <  3:104839/149‑1 (MQ=255)

                                                                                                                  aCAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGtt                                   >  5:54015/1‑149 (MQ=255)

                                                                                                                  aCAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGtt                                   <  4:421178/149‑1 (MQ=255)

                                                                                                                    aGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTgg                                 >  7:36511/1‑149 (MQ=255)

                                                                                                                      ctgcctgcCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGc                                >  6:334912/1‑148 (MQ=255)

                                                                                                                         cctgccCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGc                            >  3:214162/1‑149 (MQ=255)

                                                                                                                             cccTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGc                        >  7:11885/1‑149 (MQ=255)

                                                                                                                              ccTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGcc                       >  1:387232/1‑149 (MQ=255)

                                                                                                                              cattcgcgCCATGATGGC‑CGAAGGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGc                                >  5:225411/8‑140 (MQ=38)

                                                                                                                              cattcgcgCCATGATGGC‑CGAAGGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGc                                <  6:225411/133‑1 (MQ=38)

                                                                                                                              cattcgcgCCATGATGGC‑CGAAGGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGcc                       >  5:240845/8‑149 (MQ=38)

                                                                                                                                ttCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGTCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCg                      >  6:328033/1‑148 (MQ=255)

                                                                                                                                ttCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGcc                                      >  1:133994/1‑132 (MQ=255)

                                                                                                                                ttCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGcc                                      <  2:133994/132‑1 (MQ=255)

                                                                                                                                  cG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTa                    <  5:328033/148‑1 (MQ=255)

                                                                                                                                      ccATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAg                                                 <  4:136412/116‑1 (MQ=255)

                                                                                                                                      ccATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAg                                                 >  3:136412/1‑116 (MQ=255)

                                                                                                                                       catcatGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGa               >  2:363657/1‑149 (MQ=255)

                                                                                                                                       catcatGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGa               <  6:54015/149‑1 (MQ=255)

                                                                                                                                       catcatGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGa               >  2:76483/1‑149 (MQ=255)

                                                                                                                                            tGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGa                  <  3:175682/141‑1 (MQ=255)

                                                                                                                                            tGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGa                  >  4:175682/1‑141 (MQ=255)

                                                                                                                                              gcgcGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGt                                                                        <  7:70882/85‑1 (MQ=255)

                                                                                                                                                gcgAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCtgt      <  2:387232/149‑1 (MQ=255)

                                                                                                                                                gcgAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCtgt      <  8:61862/149‑1 (MQ=255)

                                                                                                                                                 cgAATGGCAGTTGGCGCTGTGTAGAGGATGTTTACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCtgtg     >  6:75897/1‑149 (MQ=255)

                                                                                                                                                    aTGGGAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGc   <  8:371414/148‑1 (MQ=255)

                                                                                                                                                    aTGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGcc  >  1:259925/1‑149 (MQ=255)

ACGATCGGGCAACGCTCCTTGCCGACGGTCTTGTAGTACCAGTTCCAGGCCTCAGGGTTGATCGGCTCACCCACCGACCCCAGCAGACGCAGGCTGGAACCGTCGGCGCCTTCAACAGCTGCCTGCCCTTCG‑GCCATCATGGCGCGAATGGCAGTTGGCGCTGTGTAGAGGATGTTGACCTTGTGCTTGTCGACGATCTTCGATACGCGGGTAATGTCCGGGTAGTTGGGCACGCCTTCGAACAGCAAGGTGGTGGCGCCGTTGGCCAGCGGGCCGTAGACGATATAGCTGTGGCC  >  NC_002947/5098034‑5098329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`