Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,123,458	T→A	6.6%	D225V (GAC→GTC)	PP_4510 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,123,458	0	T	A	6.6%	198.9 / 2.8	70	D225V (GAC→GTC)	PP_4510	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (35/30);  new base A (2/3);  total (37/33)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.70e-01

GGCGGCCCCCTGCCCTCGAGCCTTAAGCCGGCTCGCGCAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCGCGCCTTTCACCTTGTGCTTGAGGTAC  >  NC_002947/5123312‑5123604                                                                                                                                                   |                                                                                                                                                      ggcggcCCCCTGCCCTCGAGCCTTAAGCCGGCTCGCGCAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTc                                                                                                                                                      <  2:77284/148‑1 (MQ=255)       ccccTGCCCTCGAGCCTTAAGCCGGCTCGCGCAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTca                                                                                                                                               >  7:314487/1‑149 (MQ=255)       ccccTGCCCTCGAGCCTTAAGCCGGCTCGCGCAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGGCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTca                                                                                                                                               <  6:332937/149‑1 (MQ=255)          cTGCCCTCGAGCCTTAAGCCGGCTCGCGCAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTGGACTACCTcaatt                                                                                                                                            <  1:159444/149‑4 (MQ=255)             cccTCGAGCCTTAAGCCGGCTCGCGCAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTGGACTACCTcaattgcg                                                                                                                                         <  5:181487/149‑7 (MQ=255)             cccTCGAGCCTTAAGCCGGCTCGCGCAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTGGACTACCTcaattgcg                                                                                                                                         <  5:81574/149‑7 (MQ=255)               cTCGAGCCTTAAGCCGGCTCGCGCAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCTAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCAc                                                                                                                                                   <  3:345272/137‑1 (MQ=255)               cTCGAGCCTTAAGCCGGCTCGCGCAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCTAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCAc                                                                                                                                                   >  4:345272/1‑137 (MQ=255)               cTCGAGCCTTAAGCCGGCTCGCGCAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCACCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCtt                                                                                                                                    >  3:343343/1‑149 (MQ=255)                  gAGCCTTAAGCCGGCTCGCGCAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGc                                                                                                                                  <  1:415949/148‑1 (MQ=255)                  gAGCCTTAAGCCGGCTCGCGCAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGGGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCg                                                                                                                                 >  1:58462/1‑149 (MQ=255)                       ttAAGCCGGCTCGCGCGGCGCCGCCACGGCCTGCGGTTCGGCCAGCCGGTACAGCTGGACGCTGCCGTCCCCATGCTCGATCAGGGCCGTGCACGGCTCCCCCCAGTGCCGGCACTTGAGGTAGTCCGCCTCGC‑‑‑GCACCTTGCGGAtct                                                                                                                            >  4:351099/1‑149 (MQ=255)                       ttAAGCCGGCTCGCGCAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGAtct                                                                                                                            >  8:200937/1‑149 (MQ=255)                           gCCGGCTCGCTCAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGc                                                                                                                        <  5:238202/149‑1 (MQ=255)                             cGGCTCGCGCAACGCCGCCATGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCat                                                                                                                      >  4:328774/1‑149 (MQ=255)                               gCTCGCGCAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCatga                                                                                                                    >  7:382957/1‑149 (MQ=255)                                   gcgcAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCTGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGa                                                                                                                >  5:72079/1‑149 (MQ=255)                                   gcgcAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCAc                                                                                                                                                   <  7:223070/117‑1 (MQ=255)                                   gcgcAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCAc                                                                                                                                                   >  8:223070/1‑117 (MQ=255)                                      cAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGatat                                                                                                             >  7:263426/1‑149 (MQ=255)                                          gccgccACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGAGCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGccc                                                                                                         >  1:15131/1‑149 (MQ=255)                                            cgccACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCg                                                                                                        >  5:324068/1‑148 (MQ=255)                                             gccACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGca                                                                                                      >  1:169214/1‑149 (MQ=255)                                             gccACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGca                                                                                                      <  3:42308/149‑1 (MQ=255)                                               cACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCATGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGcaca                                                                                                    >  1:129690/1‑149 (MQ=255)                                                  ggCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCAcacca                                                                                                 >  1:106459/1‑149 (MQ=255)                                                     cTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCAccc                                                                                              >  2:32971/1‑149 (MQ=255)                                                      tGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCCC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCAcccc                                                                                             >  8:57712/1‑149 (MQ=255)                                                          cTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCGGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGa                                                                                         <  6:72079/149‑1 (MQ=255)                                                              ggCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGCCTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAAc                                                                                     >  3:220516/1‑149 (MQ=255)                                                              ggCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGaaaa                                                                                      <  5:20446/148‑1 (MQ=255)                                                               gCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCatgatg                                                                                                                  <  1:99847/119‑1 (MQ=255)                                                               gCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTCAGGTAGACCACCTCAC‑‑‑CCACCTTGCGGATCTCGCCatgatg                                                                                                                  >  2:99847/1‑119 (MQ=255)                                                                    ccGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCAcggc                                                                               <  8:302481/149‑1 (MQ=255)                                                                              tCGACACTGCCGTCCCAATGCTCGATCAGGGACGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑ACACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAACCCACGGCGGGTGCACt                                                                      >  5:115597/1‑148 (MQ=255)                                                                                gACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCAt                                                                   >  2:414111/1‑149 (MQ=255)                                                                                  cacTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATgg                                                                 <  2:106459/149‑1 (MQ=255)                                                                                   acTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATggg                                                                <  7:200937/149‑1 (MQ=255)                                                                                       ccGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAAt                                                            >  7:358402/1‑149 (MQ=255)                                                                                        cGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATg                                                           <  7:409908/149‑1 (MQ=255)                                                                                          tCCCAATGCTCGATCAGGGCCGTGCAAGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGTAAACCACGGCGGGTGCACTCATGGGCAATGGc                                                         <  6:258839/149‑1 (MQ=255)                                                                                           cccAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACAGAGCACCTTGCGGATCTCGGAATAATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATg                                                           >  1:348470/1‑149 (MQ=255)                                                                                                 gCTCGATCAGGGACGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTtctc                                                   >  8:24314/1‑148 (MQ=255)                                                                                                  cTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCTATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGca                                                                                                      <  5:386703/96‑1 (MQ=255)                                                                                                  cTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCTATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGca                                                                                                      >  6:386703/1‑96 (MQ=255)                                                                                                  cTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCg                                                  >  6:228360/1‑148 (MQ=255)                                                                                                     gATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGaa                                                <  4:380015/147‑1 (MQ=255)                                                                                                           ggCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCtga                                        >  7:99554/1‑149 (MQ=255)                                                                                                             ccGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCtgat                                       >  2:427317/1‑148 (MQ=255)                                                                                                               gTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGaa                                    <  3:351099/149‑1 (MQ=255)                                                                                                                   aCGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGtt                                 >  8:61418/1‑148 (MQ=255)                                                                                                                   aCGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGtt                                 >  2:67497/1‑148 (MQ=255)                                                                                                                            cccAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCGCGCCt                       <  8:263426/149‑1 (MQ=255)                                                                                                                             ccAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCGCGCCtt                      <  3:328774/149‑1 (MQ=255)                                                                                                                             ccAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCGCGCCtt                      >  7:260475/1‑149 (MQ=255)                                                                                                                               aGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCGCGCCTTTc                    <  8:358402/149‑1 (MQ=255)                                                                                                                                 tCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCGCGCCTTTCAc                  <  6:324068/149‑1 (MQ=255)                                                                                                                                   cccGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCGCGCCTTTCAcc                 <  7:263832/148‑1 (MQ=255)                                                                                                                                    ccGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCGCGCCTTTCACCtt               >  3:300581/1‑149 (MQ=255)                                                                                                                                     cGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGGGCTCTCATGGGCAATGGCGTTCTCGACGTCGCTGATGAAGTTCGCCGCGCCTTTCACCTtg              >  3:318914/1‑149 (MQ=255)                                                                                                                                      gCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCTCGCCTTTCACCTtgt             <  2:58462/149‑1 (MQ=255)                                                                                                                                      gCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCGCGCCTTTCACCTtgt             >  8:156123/1‑149 (MQ=255)                                                                                                                                       tgggtGAGGTGGACTACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCGCGCCTTTCACCTtgtg            >  3:121416/5‑149 (MQ=255)                                                                                                                                        aaTTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAg                                   >  2:402789/1‑125 (MQ=255)                                                                                                                                        aaTTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAg                                   <  1:402789/125‑1 (MQ=255)                                                                                                                                          ttGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCGCGCCTTTCACCTTGTGCtt         <  7:61418/149‑1 (MQ=255)                                                                                                                                             aGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCGCGCCTTTCACCTTGTGCTTGAg      <  2:348470/149‑1 (MQ=255)                                                                                                                                              ggTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCGCGCCTTTCACCTTGTGCTTGAgg     <  8:314487/149‑1 (MQ=255)                                                                                                                                               gTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCGCGCCTTTCACCTTGTGCTTGAGGt    <  2:169214/149‑1 (MQ=255)                                                                                                                                                  gTCGACCTCAC‑‑‑CCACCTTGCGGATGTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGGAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCGCGCCTTTCACCTTGTGCTTGAGGTAc  <  6:115597/148‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                      GGCGGCCCCCTGCCCTCGAGCCTTAAGCCGGCTCGCGCAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCAC‑‑‑CCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCGCGCCTTTCACCTTGTGCTTGAGGTAC  >  NC_002947/5123312‑5123604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑