| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,129,326 | A→C | 6.3% | F693C (TTC→TGC) | recQ ← | ATP‑dependent DNA 3'‑5' helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,129,326 | 0 | A | C | 6.3% | 296.2 / 7.8 | 96 | F693C (TTC→TGC) | recQ | ATP‑dependent DNA 3'‑5' helicase |
| Reads supporting (aligned to +/- strand): ref base A (48/41); new base C (2/4); total (50/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01 | |||||||||||
GTTACGCGTTCGATAATCGTCAAAAAGCATGACAAAGGTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTGGTCCAGACTCAGCTCCTGGCGGCCCAACGCCTCGGCCAGCAGGCTAT > NC_002947/5129180‑5129473 | gTTACGCGTTCGATAATCGTCAAAAAGCATGACAAAGGTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGaa < 7:374558/148‑1 (MQ=255) ttACGCGTTCGATAATCGTCAAAAAGCATGACAAAGGTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACt > 3:26883/1‑149 (MQ=255) aCGCGTTCGATAATCGTCAAAAAGCATGACAAAGGTAAGAAGATGGC‑GCTT‑CTGCCTTCGATCATGCATGCGTTTACAATTCGAATTCCGCAGCCATTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGc > 7:171436/1‑149 (MQ=255) cgcgTTCGATAATCGTCAAAAAGCATGACAAAGGTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTCCGCCCTCCGGCACCCGCGCCCCGAACTGCg < 4:67235/149‑1 (MQ=255) gATAATCGTCAAAAAGCATGACAAAGGTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGCAGTTCGGGGcgc < 4:9097/148‑3 (MQ=255) gATAATCGTCAAAAAGCATGACAAAGGTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGCAGTTCGGGGcgc < 2:277297/148‑3 (MQ=255) gATAATCGTCAAAAAGCATGACAAAGGTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAAt > 7:362817/1‑149 (MQ=255) aTAATCGTCAAAAAGCATGACAAAGGTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATg < 8:381233/149‑1 (MQ=255) aTAATCGTCAAAAAGCATGACAAAGGTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATg < 3:42396/149‑1 (MQ=255) aTAATCGTCAAAAAGCATGACAAAGGTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGAACCCGCGACCCGAACTGCGGGGCAATg > 3:127054/1‑149 (MQ=255) aTCGTCAAAAAGCATGACAAAGGTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGCAGTTCGGGGcgcgggtg < 5:298447/149‑8 (MQ=255) aTCGTCAAAAAGCATGACAAAGGTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGcc < 7:83750/149‑1 (MQ=255) tCGTCAAAAAGCATGACAAAGGTCAGCAGATGGCTGCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGcc > 1:278547/1‑149 (MQ=255) tCGTCAAAAAGCATGACAAAGGTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGCAGTTCGGGGcgcgg > 2:179715/1‑141 (MQ=255) tCGTCAAAAAGCATGACAAAGGTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGCAGTTCGGGGcgcgg < 1:179715/145‑5 (MQ=255) tCGTCAAAAAGCATGACAAAGGTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGcc > 3:211962/1‑148 (MQ=255) tCGTCAAAAAGCATGACAAAGGTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCg > 2:307436/1‑149 (MQ=255) gTCAAAAAGCATGACAAAGGTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTCCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGa > 5:201100/1‑148 (MQ=255) tCAAAAAGCATGACAAAGGTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGaca < 6:312456/149‑1 (MQ=255) cAAAAAGCATGACAAAGGTCAGCAGATGGC‑GCTTGCTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGaca > 1:278739/1‑149 (MQ=255) aaaaaaGAATGAGAAAGGTAAGCAGATGGC‑GCTT‑CTGCCTTCGATACTGCATGAGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGAGCACGAAATAGAGTACGACCTCCGGAACCCGCGACCCGAAATGCGGGGCAATGGCCGaca < 4:127054/147‑1 (MQ=255) aaaaGCATGACAAAGGTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGGTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCg < 8:362104/149‑1 (MQ=255) aaGCATGACAAAGGTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGt < 2:278547/149‑1 (MQ=255) gCATGACAAAGGTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTgg < 8:171436/149‑1 (MQ=255) aTGACAAAGGTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCa < 6:20618/149‑1 (MQ=255) gACAAAGGTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGc > 8:300790/1‑149 (MQ=255) aCAAAGGTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGc < 7:296409/148‑1 (MQ=255) aCAAAGGTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCt > 7:123807/1‑149 (MQ=255) aaaGGTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCa > 3:154348/1‑149 (MQ=255) aGGTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCAcc > 5:122380/1‑149 (MQ=255) gTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCt > 6:52913/1‑149 (MQ=255) cagcagATCGC‑GCTT‑CTGCCTTCGATCCTGAATGCCTTTACAAATCGAATTCCGCCGCCAGTTCTGCGAGAACGAAATAGAGTACGCCCTCCGGCACCCGCTCCCCGAACTGCGGGGCAATGGCCGACACCAGTGGCAGCTCACCCtag > 4:258485/1‑147 (MQ=255) cagATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCa < 5:23802/149‑1 (MQ=255) ggC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATc > 5:277293/1‑146 (MQ=255) c‑gcTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGaa < 2:278739/149‑1 (MQ=255) gcTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGaaa < 1:68674/149‑1 (MQ=255) gcTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGaaa < 3:118089/149‑1 (MQ=255) cTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAg < 8:49023/149‑1 (MQ=255) ccTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGaaa < 6:156295/142‑1 (MQ=255) ccTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGaaa > 5:156295/1‑142 (MQ=255) ccTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGCAAGGCGTcc > 4:247362/1‑149 (MQ=255) ccTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTc < 1:420621/148‑1 (MQ=255) ccTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTc < 5:52913/148‑1 (MQ=255) ccTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAATACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTc > 7:125172/1‑124 (MQ=255) ccTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAATACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTc < 8:125172/124‑1 (MQ=255) cGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTg < 6:357189/147‑1 (MQ=255) tCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCt > 8:202808/1‑149 (MQ=255) cTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTcc < 4:90991/149‑1 (MQ=255) cTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTcc < 7:8756/149‑1 (MQ=255) gCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCcat > 3:167130/1‑149 (MQ=255) cATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCcatc < 4:211962/149‑1 (MQ=255) cATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCcat > 7:198631/1‑148 (MQ=255) tGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCcatca > 8:322867/1‑148 (MQ=255) cGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCAtcatca < 2:218613/149‑1 (MQ=255) cGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCAtcatca < 1:307436/149‑1 (MQ=255) gTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAg < 5:427669/149‑1 (MQ=255) tttACAATTAGAATTCCGCCGGCTGTTCTGCGCGCACGCATTTGAGTACGCCCTCCGGCACCAGCGCGCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAgg < 3:258485/149‑1 (MQ=255) tACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAg > 4:335233/1‑146 (MQ=255) tACAATTCGAATTCCCCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAg < 3:335233/146‑1 (MQ=255) aCAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCt > 2:163649/1‑149 (MQ=255) aCAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCt > 2:80500/1‑149 (MQ=255) aaTTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTc > 7:9326/1‑149 (MQ=255) aTTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTcc < 1:174785/149‑1 (MQ=255) aTTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCACCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTcc > 2:280189/1‑149 (MQ=255) ttCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCg > 2:369937/1‑149 (MQ=255) cGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCgg < 8:9326/148‑1 (MQ=255) gAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCgg > 6:3105/1‑147 (MQ=255) gccgccAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGAt > 4:258969/1‑149 (MQ=255) aGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTg < 8:362817/149‑1 (MQ=255) tgctgcGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTGGt < 1:44505/149‑1 (MQ=255) tgcGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTGGTc > 7:52584/1‑147 (MQ=255) gcgcgcACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTGGTc > 7:197373/1‑146 (MQ=255) cgcgcACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTGGTCCa < 8:123678/147‑1 (MQ=255) cgcgcACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTGGTCCa < 7:202808/147‑1 (MQ=255) gcgcACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTGGTCCAGa > 4:69802/1‑148 (MQ=255) cgcacgcaATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTGGTCCAGACt > 8:258692/1‑149 (MQ=255) acgcaATAGAGTACGCCCTCTGGCACCCGCGCCCCGAACTGCCGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTGGTCCAGACTCAg > 4:409589/1‑149 (MQ=255) acgcaATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTGGTCCAGACTCAg < 1:163649/149‑1 (MQ=255) gcaATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTGGTCCAGACTCAGCt > 4:194318/1‑149 (MQ=255) gcaATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTGGTCCAGACTCAGCt > 6:339879/1‑149 (MQ=255) aaTAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTGGTCCAGACTCAGCTcc < 8:197373/149‑1 (MQ=255) aaTAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAA‑GCCTCCTGCACCTACATGATGAGGTATTCCCGCAGGTCGATGGCCTGGTCCAGACTCCGCTCCt > 3:252280/1‑149 (MQ=255) gTACGCCCTCCGGCACCCGCACCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTGGTCCAGACTCAGCTCCTggcgg > 2:5476/1‑149 (MQ=255) tACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTGGTCCAGACTCAGCTCCTggcggc > 7:403741/1‑149 (MQ=255) tACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTGGTCCAGACTCAGCTCCTggcggc > 7:118861/1‑149 (MQ=255) tACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTGGTCCAGACTCAGCTCCTggcgg < 6:277293/148‑1 (MQ=255) cTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTGGTCCAGACTCAGCTCCTGGCGGCCCAACg > 1:428877/1‑149 (MQ=255) cTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTGGTCCAGACTCAGCTCCTGGCGGCCCAACg > 1:392049/1‑149 (MQ=255) gcccccGCTCCCCCTTCTGCGGGGCAATGGCCGTCCCCGGGGGCATCTCTCCCTTGCCTTCTTTTTCTGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTGGTCCAGACTCAGCTCCTGGCGGCCCAACGCCTCg < 2:392049/146‑1 (MQ=255) gCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTGGTCCAGACTCAGCTCCTGGCGGCCCAACGCCTCg > 3:180861/1‑149 (MQ=255) ccGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTGGTCCAGACTCAGCTCCTGGCGGCCCAACGCCGCGGc > 6:425056/1‑147 (MQ=255) attgcCCCGCAGTTCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCGTGCTCCGCCATCCTCAGGTCTTCCGGCAGGTCGATGGCCTGGTCGAGGCTCAGCTCCTGGCGGCGCGACGCCGGGGCcag > 8:377486/4‑149 (MQ=255) gcgcCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTGGTCCAGACTCAGCTCCTGGCGGCCCAACGCCTCGGCcagc < 7:300790/149‑1 (MQ=255) cccGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTGGTCCAGACTCAGCTCCTGGCGGCCCAACGCCTCGGCCAGCAGGc > 7:356008/1‑149 (MQ=255) cGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTGGTCCAGACTCAGCTCCTGGCGGCCCAACGCCTCGGCCAGCAGGCta < 3:409589/149‑1 (MQ=255) cGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTGGTCCAGACTCAGCTCCTGGCGGCCCAACGCCTCGGCCAGCAGGCta > 7:293942/1‑149 (MQ=255) gAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTGGTCCAGACTCAGCTCCTGGCGGCCCAACGCCTCGGCCAGCAGGCtat < 8:52584/149‑1 (MQ=255) | GTTACGCGTTCGATAATCGTCAAAAAGCATGACAAAGGTCAGCAGATGGC‑GCTT‑CTGCCTTCGATCCTGCATGCGTTTACAATTCGAATTCCGCCGCCAGTGCTGCGCGCACGCAATAGAGTACGCCCTCCGGCACCCGCGCCCCGAACTGCGGGGCAATGGCCGACACCGGTGGCAGCTCACCCTCGCCATCAAGAAAGGCGTCCTGCACCTCCATCATCAGGTCTTCCGGCAGGTCGATGGCCTGGTCCAGACTCAGCTCCTGGCGGCCCAACGCCTCGGCCAGCAGGCTAT > NC_002947/5129180‑5129473 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |