Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,162,204	G→C	7.4%	W324C (TGG→TGC) ‡	PP_4544 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,162,204	0	G	C	7.4%	249.1 / 8.0	81	W324C (TGG→TGC) ‡	PP_4544	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (38/36);  new base C (3/3);  total (42/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

TCCTGCAGTCGTTCGGCAACACCACCAACGAGTTCAATCTGGTGGA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACAAGTTCAAGGTCGG  >  NC_002947/5162056‑5162350                                                                                                                                                       |                                                                                                                                                    tCCTGCAGTCGTTCGGCAACACCACCAACGAGTTCAATCTGGTGGA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCctgg                                                                                                                                                     >  4:49811/1‑149 (MQ=255)   cTGCAGTCGTTCGGCAACACCACCAACGAGTTCAATCTGGTGGA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCctggc                                                                                                                                                    <  5:330768/148‑1 (MQ=255)     gCAGTCGTTCGGCAACACCACCAACGAGTTCAATCTGGTGGA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCctggctgg                                                                                                                                                 >  4:200237/1‑149 (MQ=255)      cAGTCGTTCGGCAACACCACCAACGAGTTCAATCTGTTGGA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCctggctggc                                                                                                                                                <  8:101254/149‑1 (MQ=255)      cAGTCGTTCGGCAACACCACCAACGAGTTCAATCTGGTGGA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCctggctgg                                                                                                                                                 <  3:326686/148‑1 (MQ=255)       aGTCGTTCGGCAACACCACCAACGAGTTCAATCTGGTGGA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCctggctggct                                                                                                                                               >  3:220492/1‑149 (MQ=255)         tCGTTCGGCAACACCACCAACGAGTTCAATCTGGTGGA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCctggctggctgg                                                                                                                                             <  4:293316/149‑1 (MQ=255)           gTTCGGCAACACCACCAACGAGTTCAATCTGGTGGAGAGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCCACGGCGAAGATCACGCctgg                                                                                                                                                     >  1:289870/1‑140 (MQ=255)           gTTCGGCAACACCACCAACGAGTTCAATCTGGTGGA‑AGGCTTTGGCCAGATCGACTTGACTGGCTTGGGCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGATCACCGACAGCACCGACGGCGAAGATCAGGCctgg                                                                                                                                                     <  2:289870/139‑1 (MQ=255)           gTTCGGCAACACCACCAACGAGTTCAATCTGGTGGA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGcc                                                                                                                                           <  8:362479/149‑1 (MQ=255)              cGGCAACACCACCAACGAGTTCAATCTGGTGGA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGGCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTg                                                                                                                                         <  2:156331/148‑1 (MQ=255)               ggCAACACCACCAACGAGTTCAATCTGGTGGA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGa                                                                                                                                        >  4:299969/1‑148 (MQ=255)               ggCAACACCACCAACGAGTTCAATCTGGTGGA‑AGCCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGATGGCCTGa                                                                                                                                        >  3:194554/1‑148 (MQ=255)                gCAACACCACCAACGAGTTCAATCTGGTGGA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGaaa                                                                                                                                      <  7:398231/149‑1 (MQ=255)                gCAACACCACCAACGAGTTCAATCTGGTGGA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGaa                                                                                                                                       >  6:36394/1‑148 (MQ=255)                gCAACACCACCAACGAGTTCAATCTGGTGGA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGaa                                                                                                                                       >  8:192757/1‑148 (MQ=255)                     accaccAACGAGTTCAATCTGGTGGA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCa                                                                                                                                  >  4:419692/1‑148 (MQ=255)                     accaccAACGAGTTCAATCTGGTGGA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCa                                                                                                                                  >  8:212613/1‑148 (MQ=255)                       caccaACGAGTTCAATCTGGTGGA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCaaaa                                                                                                                               >  1:85994/1‑149 (MQ=255)                       caccaACGAGTTCAATCTGGTGGA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCAGCCAGCCAGGCCTGatcttcg                                                                                                                                  >  7:84604/1‑140 (MQ=255)                       caccaACGAGTTCAATCTGGTGGA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCAGCCAGCCAGGCCTGatcttcg                                                                                                                                  <  8:84604/146‑7 (MQ=255)                               aGTTCAATCTGGTGGA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTgcc                                                                                                                       <  7:212613/149‑1 (MQ=255)                                gTTCAATCTGGTGGA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGTTCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTgcct                                                                                                                      >  5:373887/1‑149 (MQ=255)                                        tggtggA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCTTTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTgcctggagcctg                                                                                                              <  6:415971/149‑1 (MQ=255)                                        tggtggA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTgcctggagcctg                                                                                                              >  2:192265/1‑149 (MQ=255)                                          gtggA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGATGGCCTGAAAACCAAAATGGGTgcctggagcctgga                                                                                                            <  4:194554/149‑1 (MQ=255)                                          gtggA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACATGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTgcctggagcctgga                                                                                                            <  2:4760/149‑1 (MQ=255)                                          gtggA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTAGGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTgcctggagcct                                                                                                               <  5:419224/146‑1 (MQ=255)                                           tggA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGTGTGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTgcctggagcctgga                                                                                                            >  1:90706/1‑149 (MQ=255)                                             gA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑a                                                                                                        >  2:429566/1‑149 (MQ=255)                                                         ccAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACcgc                                                                                              >  7:108822/1‑148 (MQ=255)                                                         ccAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACcgc                                                                                              >  6:314470/1‑148 (MQ=255)                                                          cAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGa                                                                                            >  7:71072/1‑149 (MQ=255)                                                          cAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGa                                                                                            >  8:229984/1‑149 (MQ=255)                                                           aGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACcgcg                                                                                             <  1:218667/147‑1 (MQ=255)                                                           aGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACcgcg                                                                                             >  2:218667/1‑147 (MQ=255)                                                           aGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGAt                                                                                           <  4:220492/149‑1 (MQ=255)                                                           aGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGAt                                                                                           >  1:41489/1‑149 (MQ=255)                                                             aTCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGAtgt                                                                                         <  4:37803/149‑1 (MQ=255)                                                              tCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGAtgtg                                                                                        >  7:136961/1‑149 (MQ=255)                                                              tCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGAtgtg                                                                                        <  8:108822/149‑1 (MQ=255)                                                              tCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATAGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGAtgtg                                                                                        <  1:413648/149‑1 (MQ=255)                                                                gACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCa                                                                                      <  8:71072/149‑1 (MQ=255)                                                                 aCTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAg                                                                                     >  6:240469/1‑149 (MQ=255)                                                                          gCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAAcgcg                                                                            >  1:202721/1‑149 (MQ=255)                                                                          gCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAAcg                                                                              <  2:85994/147‑1 (MQ=255)                                                                              ggCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCggtg                                                                         <  3:200237/148‑1 (MQ=255)                                                                               gCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCggtggt                                                                       <  2:41283/149‑1 (MQ=255)                                                                               gCCATTCCGT‑TGTCGGCCTATGGCCAGTACCTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCggtggt                                                                       >  1:41283/1‑149 (MQ=255)                                                                                     ccGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCAGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGcc                                                                  <  5:36394/148‑1 (MQ=255)                                                                                      cGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGAGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTg                                                                >  3:135815/1‑149 (MQ=255)                                                                                        t‑tGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGtt                                                              <  6:373887/149‑1 (MQ=255)                                                                                           gTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTc                                                             <  3:49811/148‑1 (MQ=255)                                                                                            tCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCAc                                                           <  5:150941/149‑1 (MQ=255)                                                                                               gCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCggtgg                                                                        <  7:399702/133‑1 (MQ=255)                                                                                               gCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCggtgg                                                                        >  8:399702/1‑133 (MQ=255)                                                                                                   caagCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCg                                                    <  8:192989/146‑1 (MQ=255)                                                                                                   aTGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCg                                                    >  1:55748/1‑149 (MQ=255)                                                                                                    tGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCg                                                    <  2:274359/148‑1 (MQ=255)                                                                                                      gCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGa                                                  <  7:229984/148‑1 (MQ=255)                                                                                                      gCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGAc                                                 <  2:90706/149‑1 (MQ=255)                                                                                                         aGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTc                                              >  2:8976/1‑149 (MQ=255)                                                                                                          gTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCg                                                    <  4:225238/142‑1 (MQ=255)                                                                                                          gTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCg                                                    >  3:225238/1‑142 (MQ=255)                                                                                                              gTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCAc                                                           >  4:312760/1‑131 (MQ=255)                                                                                                              gTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCAc                                                           <  3:312760/131‑1 (MQ=255)                                                                                                               tGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAAc                                        >  2:259659/1‑149 (MQ=255)                                                                                                                gaagaaCACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACg                                       >  1:118934/1‑149 (MQ=255)                                                                                                                gaagaaCACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACg                                       >  4:234097/1‑149 (MQ=255)                                                                                                                     acacCGACAGCGCCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTac                                  <  2:123847/149‑1 (MQ=255)                                                                                                                       acCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTacac                                >  5:434779/1‑149 (MQ=255)                                                                                                                        ccGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACAcc                               >  7:27552/1‑149 (MQ=255)                                                                                                                            cAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGtt                           <  3:419692/149‑1 (MQ=255)                                                                                                                                aCCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCg                              >  6:300658/1‑142 (MQ=255)                                                                                                                                aCCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCg                              <  5:300658/142‑1 (MQ=255)                                                                                                                                 ccGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTcgcgc                      <  2:332702/149‑1 (MQ=255)                                                                                                                                      ggCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACTAACAAATACCGCGATTTGCAGCGAAACGCGTTGGTCAGCGTGTTCACCCATTCGGAATTCGGCAACGGCTAAACCGGTTCGCGCGGt                   >  1:124891/1‑148 (MQ=255)                                                                                                                                       gCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTcac                <  7:192757/149‑1 (MQ=255)                                                                                                                                         gAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTcac                >  3:133460/1‑147 (MQ=255)                                                                                                                                         gAAGATCAGGCCTGACTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTcac                >  4:140973/1‑147 (MQ=255)                                                                                                                                            tttCAGGCCAGCCAGCCAGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCg                                             <  8:178718/113‑1 (MQ=255)                                                                                                                                            tttCAGGCCAGCCAGCCAGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCg                                             >  7:178718/3‑115 (MQ=255)                                                                                                                                            tttCAGGCCAGCCAGCCAGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAAc                                        <  2:121931/118‑1 (MQ=255)                                                                                                                                            tttCAGGCCAGCCAGCCAGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAAc                                        >  1:121931/3‑120 (MQ=255)                                                                                                                                              tCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCgg                                                   <  1:352430/107‑1 (MQ=255)                                                                                                                                              tCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCgg                                                   >  2:352430/1‑107 (MQ=255)                                                                                                                                                aGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACAAGTTCAAg       <  6:308167/149‑1 (MQ=255)                                                                                                                                                  gCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACAAGTTCAAGGt     >  8:53044/1‑149 (MQ=255)                                                                                                                                                  gCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACAAGTTCAAGGt     >  6:373656/1‑149 (MQ=255)                                                                                                                                                   ccTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACAAGTTCAAGGTc    <  8:27552/149‑1 (MQ=255)                                                                                                                                                    ctggctggctggCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACAAGTTCAAGGTCg   <  5:314470/149‑1 (MQ=255)                                                                                                                                                     tggctggctggcCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACAAGTTCAAGGTCgg  >  1:98717/1‑149 (MQ=255)                                                                                                                                                       |                                                                                                                                                    TCCTGCAGTCGTTCGGCAACACCACCAACGAGTTCAATCTGGTGGA‑AGGCTTTGGCCAGATCGACTTCACTGGCTTGGCCATTCCGT‑TGTCGGCCTATGGCCAGTACGTGAAGAACACCGACAGCACCGACGGCGAAGATCAGGCCTGGCTGGCTGGCCTGAAAACCAAAATGGGTGCCTGGAGCCTGGACT‑ACAACTACCGCGATGTGCAGCGTAACGCGGTGGTCAGCCTGTTCACCGATTCGGACTTCGGCAACGGCTACACCGGTTCGCGCGGTCACAAGTTCAAGGTCGG  >  NC_002947/5162056‑5162350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑