Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,174,514 G→T 6.0% L156M (CTG→ATG)  fadD‑II ← long‑chain‑fatty acid‑‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,174,5140GT 6.0% 213.7 / 3.6 67L156M (CTG→ATG) fadD‑IIlong‑chain‑fatty acid‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base G (35/28);  new base T (2/2);  total (37/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

TTCAGCGACACCGGTTTGGGCGACAGCCCGCGGCCCTCGCGCAGCACCTGCTTGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCGAACATGTTCAGGTACACCAGCGCACGCGCGCCACTGTCCTTGAACTGGTGGCGCATCTCGCGCTCGGTGTACAGCGG  >  NC_002947/5174366‑5174661
                                                                                                                                                     |                                                                                                                                                   
ttCAGCGACACCGGTTTGGGCGACAGCCCGCGGCCCTCGCGCAGCACCTGCTTGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGcagcag                                                                                                                                                     <  5:417636/149‑1 (MQ=255)
    gCGACACCGGTTTGGGCGACAGCCCGCGGCCCTCGCGCAGCACCTGCTTGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCa                                                                                                                                                 >  6:346451/1‑149 (MQ=255)
     cGACCCCGGTTTGGGCGACAGCCCGCGGCCCTCGCGCAGCACCTGCTTGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCAc                                                                                                                                                <  7:350287/149‑1 (MQ=255)
     cGACACCGGTTTGGGCGACAGCCCGCGGCCCTCGCGCAGCACCTGCTTGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCCGCCCTTGGCGGCCGGCAGCAGGTccc                                                                                                                                                >  2:99131/1‑147 (MQ=255)
     cGACACCGGTTTGGGCGACAGCCCGCGGCCCTCACGCAGCACCTGCTTGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCAc                                                                                                                                                <  7:229631/149‑1 (MQ=255)
      gACACCGGTTTGGGCGACAGCCCGCGGCCCTCGCGCAGCACCTGCTTGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCAcc                                                                                                                                               >  6:437850/1‑149 (MQ=255)
       acacCGGTTTGGGCGACAGCCCGCGGCCCTCGCGCAGCACCTGCTTGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCAcc                                                                                                                                               >  3:398448/1‑148 (MQ=255)
         acCGGTTTGGGCGACAGCCCGCGGCCCTCGCGCAGCACCTGCTTGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCAt                                                                                                                                            >  5:365119/1‑149 (MQ=255)
         acCGGTTTGGGCGACAGCCCGCGGCCCTCGCACAGCACCTGCTTGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCAt                                                                                                                                            >  1:45488/1‑149 (MQ=255)
          ccGGTTTGGGCGACAGCCCGCGGCCCTCGCGCAGCACCTGCTTGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATc                                                                                                                                           >  8:217782/1‑149 (MQ=255)
           cGGTTTGGGCGACAGCCCGCGGCCCTCGCGCAGCACCTGCTTGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCt                                                                                                                                          >  2:15068/1‑149 (MQ=255)
                   gCGACAGCCCGCGGCCCTCGCGCAGCACCTGCTTGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTc                                                                                                                                   >  7:360995/1‑148 (MQ=255)
                   gCGACAGCCCGCGGCCCTCGCGCAGCACCTGCTTGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGAACAGCCAGCCCTTGGCGGCCGGCAGCAGGTCCCCCATCTTTGCCTc                                                                                                                                   >  5:54569/1‑148 (MQ=255)
                       cAGCCCGCGGCCCTCGCGCAGCACCTGCTTGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCa                                                                                                                              <  1:69185/149‑1 (MQ=255)
                        aGCCCGCGGCCCTCGCGCAGCACCTGCTTGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAg                                                                                                                             <  5:134090/149‑1 (MQ=255)
                             gcgGCCCTCGCGCAGCACCTGCTTGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTAtt                                                                                                                        <  6:185957/149‑1 (MQ=255)
                              cgGCCCTCGCGCAGCACCTGCTTGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTc                                                                                                                       <  5:69591/149‑1 (MQ=255)
                                      gcgcAGCACCTGCTTGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCg                                                                                                                <  6:365119/148‑1 (MQ=255)
                                       cgcAGCACCTGCTTGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCgg                                                                                                               >  4:354660/1‑148 (MQ=255)
                                          agcaCCTGCTTGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATc                                                                                                           <  4:396966/149‑1 (MQ=255)
                                             aCCTGCTTGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCggg                                                                                                        <  5:437850/149‑1 (MQ=255)
                                               cTGCTTGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCggg                                                                                                        >  1:150905/1‑147 (MQ=255)
                                               cTGCTTGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCggg                                                                                                        >  7:385600/1‑147 (MQ=255)
                                                 gCTTGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGgcag                                                                                                     >  8:332913/1‑148 (MQ=255)
                                                  cTTGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGgcagc                                                                                                    <  3:354660/148‑1 (MQ=255)
                                                   ttGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCAc                                                                                                  >  6:386371/1‑149 (MQ=255)
                                                   ttGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCAc                                                                                                  >  6:32734/1‑149 (MQ=255)
                                                    tGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCATGTCACCCATCTTTGCCTCGATCAGGTATTCGATAc                                                                                                                  >  2:397464/1‑132 (MQ=255)
                                                    tGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCATGTCACCCATCTTTGCCTCGATCAGGTATTCGATAc                                                                                                                  >  2:391728/1‑132 (MQ=255)
                                                    tGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCATGTCACCCATCTTTGCCTCGATCAGGTATTCGATAc                                                                                                                  <  1:397464/132‑1 (MQ=255)
                                                    tGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCATGTCACCCATCTTTGCCTCGATCAGGTATTCGATAc                                                                                                                  <  1:391728/132‑1 (MQ=255)
                                                      aaCGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCAcctc                                                                                               <  5:376186/149‑1 (MQ=255)
                                                      aaCGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCAcctc                                                                                               >  4:353019/1‑149 (MQ=255)
                                                         ggCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGGTTGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCAcctcct                                                                                             >  1:125390/1‑149 (MQ=255)
                                                         ggCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTg                                                                                            <  8:360995/149‑1 (MQ=255)
                                                           cACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCa                                                                                          >  3:23115/1‑149 (MQ=255)
                                                           cACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCa                                                                                          >  2:49647/1‑149 (MQ=255)
                                                            aCCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCAc                                                                                         <  8:56504/149‑1 (MQ=255)
                                                             ccGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCAcg                                                                                        <  2:45488/149‑1 (MQ=255)
                                                              cGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCAcgc                                                                                       <  7:217782/149‑1 (MQ=255)
                                                                  tGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGCTCAGGAATTCGATACCGGTATCGGGCAGCACCTCCTGCCCGCGCtt                                                                                   >  6:441890/1‑149 (MQ=255)
                                                                     ggCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGcc                                                                                >  8:335969/1‑149 (MQ=255)
                                                                      gCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGcc                                                                                >  7:324869/1‑148 (MQ=255)
                                                                       cAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCg                                                                               >  4:8773/1‑148 (MQ=255)
                                                                       cAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCg                                                                               >  7:121818/1‑148 (MQ=255)
                                                                         aCCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCGAAc                                                                            <  6:264610/149‑1 (MQ=255)
                                                                           cGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCGAACAt                                                                          <  6:214232/149‑1 (MQ=255)
                                                                                 gCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCGAACATGTTCAg                                                                    <  7:335969/149‑1 (MQ=255)
                                                                                  ccGGCACCATCTTCTTCAACTTATCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCGAACATGTTCAgg                                                                   <  5:184259/149‑1 (MQ=255)
                                                                                       aCCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCGAACATGTTCAGGTacac                                                              >  1:273924/1‑149 (MQ=255)
                                                                                         cATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCGAACATGTTCAGGTACACCa                                                            <  3:353019/149‑1 (MQ=255)
                                                                                               cttcAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCGAACATGTTCAGGTACACCAgcgc                                                        <  7:332913/147‑1 (MQ=255)
                                                                                                ttcAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCGAACATGTTCAGGTACACCAGCGCAcg                                                     >  7:234032/1‑149 (MQ=255)
                                                                                                 tcAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCGAACATGTTCAGGTACACCAGCGCAcgc                                                    >  1:310603/1‑149 (MQ=255)
                                                                                                  cAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCGAACATGTTCAGGTACACCAGCGCAcgcg                                                   >  3:67439/1‑149 (MQ=255)
                                                                                                     cTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCGAACATGTTCAGGTACACCAGCGCACgcgcgc                                                <  8:385600/149‑1 (MQ=255)
                                                                                                     cTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCGAACATGTTCAGGTACACCAGCGCACgcgcgc                                                <  2:150905/149‑1 (MQ=255)
                                                                                                       tGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCGAACATGt                                                                        >  4:348301/1‑123 (MQ=255)
                                                                                                       tGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCGAACATGt                                                                        <  3:348301/123‑1 (MQ=255)
                                                                                                           gACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACACGCTTGCCGAACATGTTCAGGTACACCAGCGCACGCGCGCCACTGt                                          >  1:145893/1‑149 (MQ=255)
                                                                                                            aCCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCGAACATGTTCAGGTACACCAGCGCACGCGCGCCACTGTc                                         <  5:386371/149‑1 (MQ=255)
                                                                                                              cACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCGAACATGTTCAGGTACACCAGCGCACGCGCGCCACTGTCCt                                       >  2:252071/1‑149 (MQ=255)
                                                                                                                cGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGGAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCGAACATGTTCAGGTACACCAGCGCa                                                       >  1:285890/1‑131 (MQ=255)
                                                                                                                cGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCGAACATGTTCAGGTACACCAGCGCa                                                       <  2:285890/131‑1 (MQ=255)
                                                                                                                             gccagccCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCGAACATGTTCAGGTACACCAGCGCACGCGCGCCACTGTCCTTGAACTGGTGgcgc                         <  1:15068/149‑1 (MQ=255)
                                                                                                                              ccagccCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCGAACATGTTCAGGTACACCAGCGCACGCGCGCCACTGTCCTTGAACTGGTGGCGCa                        >  1:415575/1‑149 (MQ=255)
                                                                                                                                   ccTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCGAACATGTTCAGGTACACCAGCGCACGCGCGCCACTGTCCTTGAACTGGTGGCGCATCTcg                   <  3:8773/149‑1 (MQ=255)
                                                                                                                                              ggcAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCGAACATGTTCAGGTACACCAGCGCACGCGCGCCACTGTCCTTGAACTGGTGGCGCATCTCGCGCTCGGTGTa        >  7:239698/1‑149 (MQ=255)
                                                                                                                                                  gcagGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCGAACATGTTCAGGTACACCAGCGCACGCGCGCCACTGTCCTTGAACTGGTGGCGCATCTCGCGCTCGGTGTACAGc    <  2:368671/149‑1 (MQ=255)
                                                                                                                                                   cagGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCGAACATGTTCAGGTACACCAGCGCACGCGCGCCACTGTCCTTGAACTGGTGGCGCATCTCGCGCTCGGTGTACAGCg   <  4:67439/149‑1 (MQ=255)
                                                                                                                                                    agGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCGAACATGTTCAGGTACACCAGCGCACGCGCGCCACTGTCCTTGAACTGGTGGCGCATCTCGCGCTCGGTGTACAGCgg  <  5:346451/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TTCAGCGACACCGGTTTGGGCGACAGCCCGCGGCCCTCGCGCAGCACCTGCTTGAACGGCACCGCCTGGGGCAACCGGTAGGCCGGCACCATCTTCTTCAACTTGTCGACCACGG‑TGTTGACCAGCCAGCCCTTGGCGGCCGGCAGCAGGTCACCCATCTTTGCCTCGATCAGGTATTCGATACCGGTATCGGGCAGCACCTCCTGCACGCGCTTGCCGAACATGTTCAGGTACACCAGCGCACGCGCGCCACTGTCCTTGAACTGGTGGCGCATCTCGCGCTCGGTGTACAGCGG  >  NC_002947/5174366‑5174661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑