Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	396,856	C→G	6.8%	L30L (CTG→CTC) ‡	PP_0329 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	396,856	0	C	G	6.8%	284.1 / 8.6	88	L30L (CTG→CTC) ‡	PP_0329	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (40/42);  new base G (4/2);  total (44/44)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.37e-01

TATCCAAAGGGGGTGTAGGTGTGATTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGAAATAGATGGCCTGGGCAC  >  NC_002947/396710‑396997                                                                                                                                                    |                                                                                                                                               tATCCAAAGGGGGTGTAGGTGTGATTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAg                                                                                                                                              <  7:173519/149‑1 (MQ=255)   tCCAAAGGGGGTGTAGGTGTGATTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAgg                                                                                                                                             <  5:367319/148‑1 (MQ=255)     cAAAGGGGGTGTAGGTGTGATTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGAtt                                                                                                                                          <  1:229788/149‑1 (MQ=255)      aaaGGGGGTGTAGGTGTGATTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGAtt                                                                                                                                          >  5:425594/1‑148 (MQ=255)      aaaGGGGGTGTAGGTGTGATTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGAtt                                                                                                                                          >  3:111535/1‑148 (MQ=255)      aaaGGGGGTGTAGGTGTGATTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGAtt                                                                                                                                          >  8:392804/1‑148 (MQ=255)           gggTGTAGGTGTGATTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGc                                                                                                                                   <  7:391373/149‑1 (MQ=255)             gtgtAGGTGTGATTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTT‑CAATCCTGCGGATTGGAACGCtc                                                                                                                                 <  1:176971/149‑2 (MQ=255)              tgtAGGTGTGATTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTc                                                                                                                                <  6:269855/149‑1 (MQ=255)                        aTTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGttt                                                                                                                       <  3:115837/148‑1 (MQ=255)                          ttttttGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTcc                                                                                                                     >  5:105844/1‑148 (MQ=255)                            ttttGCTTTCCGTCAGGCGTTCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGTAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCaa                                                                                                                   <  6:187997/148‑1 (MQ=255)                             tttGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAAt                                                                                                                  <  3:30405/148‑1 (MQ=255)                               tGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGa                                                                                                                >  5:251835/1‑148 (MQ=255)                               tGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGa                                                                                                                <  6:425594/148‑1 (MQ=255)                                gCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGa                                                                                                                >  3:400383/1‑147 (MQ=255)                                gCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCAAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGa                                                                                                                >  2:237210/1‑147 (MQ=255)                                 cTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAGTCTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGa                                                                                                                >  1:119751/1‑147 (MQ=255)                                 cTTTCCGTCAGGCGTGCTAACGACAAGCACTGAGTCCATCTCATCTGTTGCTAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCTTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACa                                                                                                              <  2:352560/148‑1 (MQ=255)                                  tttCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGt                                                                                                            >  2:438044/1‑149 (MQ=255)                                   ttCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTg                                                                                                           >  5:325310/1‑149 (MQ=255)                                    tCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGACCTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTg                                                                                                           <  1:30587/148‑1 (MQ=255)                                       gTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCa                                                                                                       <  5:157267/149‑1 (MQ=255)                                            gCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTg                                                                                                  >  3:339231/1‑149 (MQ=255)                                             cGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGc                                                                                                 <  7:392804/149‑1 (MQ=255)                                               tGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCa                                                                                                                                               <  4:236207/102‑1 (MQ=255)                                               tGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCa                                                                                                                                               >  3:236207/1‑102 (MQ=255)                                               tGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGctg                                                                                               <  2:119751/149‑1 (MQ=255)                                                  tCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTTGTCAGTTTGctggtc                                                                                            >  4:262728/1‑149 (MQ=255)                                                  tCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGctggtc                                                                                            >  5:64892/1‑149 (MQ=255)                                                  tCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGctggtc                                                                                            >  2:379175/1‑149 (MQ=255)                                                    aCGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGctggtctg                                                                                          <  6:251835/149‑1 (MQ=255)                                                       aCAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGctggtctgg                                                                                         >  5:387002/1‑147 (MQ=255)                                                             aCTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAata                                                                                 >  1:435956/1‑149 (MQ=255)                                                               tGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATAtt                                                                               >  1:153784/1‑149 (MQ=255)                                                               tGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTCGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGctggtctggt                                                                                        <  5:176618/140‑1 (MQ=255)                                                               tGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTCGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGctggtctggt                                                                                        >  6:176618/1‑140 (MQ=255)                                                                  gTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTgg                                                                            <  6:64892/149‑1 (MQ=255)                                                                     catctcatctGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTgggg                                                                          <  1:379175/148‑1 (MQ=255)                                                                     catctcatctGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTgggg                                                                          <  4:400383/148‑1 (MQ=255)                                                                           atctGTTGCGAAAAG‑CTGGTAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCa                                                                    <  2:322787/148‑1 (MQ=255)                                                                           atctGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACa                                                                                                              <  7:249401/106‑1 (MQ=255)                                                                           atctGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACa                                                                                                              >  8:249401/1‑106 (MQ=255)                                                                           atctGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAg                                                                   >  4:71318/1‑149 (MQ=255)                                                                              tGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGAc                                                                >  8:144608/1‑149 (MQ=255)                                                                               gTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACt                                                               >  1:407518/1‑149 (MQ=255)                                                                                ttGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACt                                                               <  2:435956/148‑1 (MQ=255)                                                                                   cGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGttt                                                           <  4:111535/149‑1 (MQ=255)                                                                                   cGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGttt                                                           <  6:325310/149‑1 (MQ=255)                                                                                     aaaaG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGttttt                                                         <  6:105844/149‑1 (MQ=255)                                                                                      aaaG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTc                                                        <  4:169878/149‑1 (MQ=255)                                                                                            tGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGa                                                   >  2:124149/1‑149 (MQ=255)                                                                                             gggAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGAc                                                  >  6:223847/1‑149 (MQ=255)                                                                                              ggAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGAc                                                  <  3:71318/148‑1 (MQ=255)                                                                                               gAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAAACCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGt                                                >  1:102967/1‑149 (MQ=255)                                                                                                aaCTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTg                                               >  1:424483/1‑149 (MQ=255)                                                                                                   ttCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTc                                            >  1:414529/1‑149 (MQ=255)                                                                                                   ttCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTc                                            >  2:69636/1‑149 (MQ=255)                                                                                                     ccTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTc                                            <  5:223847/147‑1 (MQ=255)                                                                                                      cTGCTTCTTTGCCAGCGGACTAAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCg                                         >  4:3466/1‑149 (MQ=255)                                                                                                        gCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGtt                                       >  8:70548/1‑149 (MQ=255)                                                                                                              ttGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCa                                                                                                       >  1:268663/1‑79 (MQ=255)                                                                                                              ttGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCa                                                                                                       <  2:268663/79‑1 (MQ=255)                                                                                                               tGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCt                                   <  4:339231/146‑1 (MQ=255)                                                                                                                  ctgCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGtt                             >  6:430617/3‑149 (MQ=255)                                                                                                                   aGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTg                            <  6:387002/149‑1 (MQ=255)                                                                                                                    gCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTgg                           <  1:438044/149‑1 (MQ=255)                                                                                                                        aCTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTc                                            <  6:195568/128‑1 (MQ=255)                                                                                                                        aCTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTc                                            >  5:195568/1‑128 (MQ=255)                                                                                                                          tCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCgg                     <  4:423302/149‑1 (MQ=255)                                                                                                                            aGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCgg                     <  3:262728/147‑1 (MQ=255)                                                                                                                             gCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGaaa                  >  4:10894/1‑149 (MQ=255)                                                                                                                              cAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGt                                     <  6:368227/129‑1 (MQ=255)                                                                                                                              cAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGt                                     >  5:368227/1‑129 (MQ=255)                                                                                                                              cAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGAAAt                 <  2:407518/149‑1 (MQ=255)                                                                                                                                agagGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGAAATAg               <  2:424483/149‑1 (MQ=255)                                                                                                                                 gagGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGaaa                  >  6:387164/1‑145 (MQ=255)                                                                                                                                   gggAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGtt                                       <  7:321488/122‑1 (MQ=255)                                                                                                                                   gggAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGtt                                       >  8:321488/1‑122 (MQ=255)                                                                                                                                   gggAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGAAATAGATg            <  2:342285/149‑1 (MQ=255)                                                                                                                                    gaaGCGTT‑CAATCCTGCGGATTGGAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGAAATAGATgg           >  7:64209/3‑149 (MQ=255)                                                                                                                                    gaaGCGTT‑CAATCCTGCGGATTGGAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGAAATAGATgg           >  3:324127/3‑149 (MQ=255)                                                                                                                                     aaGCGTT‑CAATCCTGCGGATTGGAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGaa                   >  4:86229/2‑140 (MQ=255)                                                                                                                                     aaGCGTT‑CAATCCTGCGGATTGGAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGaa                   <  3:86229/139‑1 (MQ=255)                                                                                                                                     aaGCGTT‑CAATCCTGCGGATTGGAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCGCCCGGTGTCTGCAGTTGGCCCCGGAAATAGATGGc          >  8:113789/2‑149 (MQ=255)                                                                                                                                      aGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGAAATAGATGGcc         >  2:26220/1‑149 (MQ=255)                                                                                                                                       gCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGAAATAGATGGCCt        <  3:10894/149‑1 (MQ=255)                                                                                                                                              aaTCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGAAATAGATGGCCTGGGCAc  >  2:76940/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                               TATCCAAAGGGGGTGTAGGTGTGATTTTTTTGCTTTCCGTCAGGCGTGCTCACGACAAGCACTGAGTCCATCTCATCTGTTGCGAAAAG‑CTGGGAACTTCCTGCTTCTTTGCCAGCGGACTCAGCAAGAGGGAGCGTTCCAATCCGCAGGATT‑GAACGCTTCTGATCGTTTCCAATGACAGTGGTCAGTTTGCTGGTCTGGTAGAAATATTTGGGGGTATCAGGACTGTTTTTCACCGACGTGCTCCCGTTGTCTGCAGTTGGCCCCGGAAATAGATGGCCTGGGCAC  >  NC_002947/396710‑396997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑