Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,184,127	G→T	6.0%	Q88H (CAG→CAT)	PP_4564 →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,184,127	0	G	T	6.0%	279.1 / 3.4	89	Q88H (CAG→CAT)	PP_4564	lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (39/43);  new base T (3/3);  total (43/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAGGCCTTCCGCGAA  >  NC_002947/5183980‑5184274                                                                                                                                                    |                                                                                                                                                     gCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTATCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAg                                                                                                                                                      <  1:307518/148‑1 (MQ=255) gCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAgg                                                                                                                                                     >  2:410300/1‑149 (MQ=255)  cTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGt                                                                                                                                                    >  6:115422/1‑149 (MQ=255)     aGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACCTGGTCGTCGGCGACCATGTc                                                                                                                                                   <  5:103607/147‑1 (MQ=255)     aGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCa                                                                                                                                                  <  3:21023/148‑1 (MQ=255)      gCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCAcc                                                                                                                                                <  5:199509/149‑1 (MQ=255)         aCTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCTGCTACGTGACATGGTCGCCGACGACCACGTCACCgcc                                                                                                                                             >  1:333904/1‑147 (MQ=255)         aCTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑c                                                                                                                                            <  2:54186/149‑1 (MQ=255)             ccTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCa                                                                                                                                          <  4:63635/147‑1 (MQ=255)                 gACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGccc                                                                                                                                     <  1:112659/148‑1 (MQ=255)                  aCATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTg                                                                                                                                   <  1:111365/149‑1 (MQ=255)                   cATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGCCCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTg                                                                                                                                   >  8:311079/1‑148 (MQ=255)                    aTGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTg                                                                                                                                   >  6:439505/1‑147 (MQ=255)                    aTGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTg                                                                                                                                   >  2:92227/1‑147 (MQ=255)                     tGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAgg                                                                                                                                        <  8:129294/141‑1 (MQ=255)                     tGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAgg                                                                                                                                        >  7:129294/1‑141 (MQ=255)                      gAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGACTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTgcag                                                                                                                                >  5:95098/1‑148 (MQ=255)                       aaCCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTgcagc                                                                                                                               >  8:23099/1‑148 (MQ=255)                        aCCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCaa                                                                                                                             <  5:115422/149‑1 (MQ=255)                         ccGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGt                                                                                                                                                    <  1:294258/126‑1 (MQ=255)                         ccGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGt                                                                                                                                                    >  2:294258/1‑126 (MQ=255)                         ccGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCGACGTGACATGGGCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCaaa                                                                                                                            <  6:117039/149‑1 (MQ=255)                          cgcgAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAg                                                                                                                           >  7:169995/1‑149 (MQ=255)                            cgtgctaaGGGCGCTTCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTGGCGGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAATGCa                                                                                                                         <  2:333904/143‑1 (MQ=255)                                          tgctgGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGt                                                                                                           >  6:377288/1‑149 (MQ=255)                                            ctgGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTg                                                                                                          <  2:14498/148‑1 (MQ=255)                                            ctgGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGa                                                                                                         <  6:159571/149‑1 (MQ=255)                                             tgGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGa                                                                                                         <  1:290632/149‑1 (MQ=255)                                              ggATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAgg                                                                                                       >  2:385769/1‑149 (MQ=255)                                              ggATGATCGGTTGCGCGAGCACCTGACCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAgg                                                                                                       >  5:203104/1‑149 (MQ=255)                                               gatgatCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTgca                                                                                                                                 <  1:307170/122‑1 (MQ=255)                                               gatgatCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTgca                                                                                                                                 >  2:307170/1‑122 (MQ=255)                                               gatgatCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAgg                                                                                                       <  1:385769/148‑1 (MQ=255)                                                      ggTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGCCGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCCAGGTCCGTGAGGCAATTGGt                                                                                               >  8:348885/1‑149 (MQ=255)                                                      ggTGGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGt                                                                                               <  4:300735/149‑1 (MQ=255)                                                       gTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGGCCGGGTAACTAGAGTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTGACCGA‑CCAGGCCGTGCAGCAAAGCACCCGCGAGGTCGGTGAGGCAATTGGTc                                                                                              >  7:272786/1‑149 (MQ=255)                                                       gTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTc                                                                                              >  1:188588/1‑149 (MQ=255)                                                       gTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGACGCAATTGGTc                                                                                              >  7:270559/1‑149 (MQ=255)                                                           cgcgAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGAtt                                                                                          <  7:23099/149‑1 (MQ=255)                                                           cgcgAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGAtt                                                                                          <  5:109355/149‑1 (MQ=255)                                                           cgcgAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGAtt                                                                                          <  4:178382/149‑1 (MQ=255)                                                            gcgAGCACCTGGCCTGGCATTGCAAGACTCCACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGGGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTg                                                                                         <  4:242945/149‑1 (MQ=255)                                                                   cctggcctggCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAgg                                                                                  <  2:301517/149‑1 (MQ=255)                                                                    ctggcctggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAgg                                                                                  >  8:84277/1‑148 (MQ=255)                                                                       gcctggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGACTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGaaa                                                                               <  6:95098/148‑1 (MQ=255)                                                                         ctggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCAACGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCa                                                                            >  2:189837/1‑149 (MQ=255)                                                                          tggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCAc                                                                           >  7:19058/1‑149 (MQ=255)                                                                            gcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCAccc                                                                         >  5:127037/1‑149 (MQ=255)                                                                            gcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCAcc                                                                          <  1:410300/148‑1 (MQ=255)                                                                                 gCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCg                                                                    >  8:371213/1‑149 (MQ=255)                                                                                  cAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGTCATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGa                                                                                    <  1:44089/132‑1 (MQ=255)                                                                                  cAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGTCATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGa                                                                                    >  2:44089/1‑132 (MQ=255)                                                                                  cAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGGCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCgg                                                                   <  7:128058/149‑1 (MQ=255)                                                                                   aaGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGt                                                                                                           >  6:167241/1‑108 (MQ=255)                                                                                       cTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCAcc                                                              >  5:366026/1‑149 (MQ=255)                                                                                          aaCTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGaa                                                           >  3:331454/1‑149 (MQ=255)                                                                                           aCTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAAc                                                          <  6:234554/149‑1 (MQ=255)                                                                                           aCTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAAc                                                          <  6:240598/149‑1 (MQ=255)                                                                                             tGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCAAGGAAATCACCCCTTCGGCCACCGAActg                                                        >  1:61530/1‑149 (MQ=255)                                                                                                ccGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGACctgctg                                                     >  2:87434/1‑149 (MQ=255)                                                                                                ccGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAActgctg                                                     >  3:21963/1‑149 (MQ=255)                                                                                                 cGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACtgctgc                                                    >  1:328344/1‑149 (MQ=255)                                                                                                 cGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAAACACCCCTTCGGCCACCGAACtgctgc                                                    >  1:65327/1‑149 (MQ=255)                                                                                                        cTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCAt                                             <  4:21963/149‑1 (MQ=255)                                                                                                         tCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTCCTGCGCGGCAt                                             >  4:315705/1‑148 (MQ=255)                                                                                                            aCTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCACGAGc                                         <  5:377288/149‑1 (MQ=255)                                                                                                                gCTCGACCGGCTACGTGACAGGGTCGCCGACGCCCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGa                                       <  8:307155/147‑1 (MQ=255)                                                                                                                  tCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAAc                                   >  7:375136/1‑149 (MQ=255)                                                                                                                    gACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAAcc                                  >  6:364971/1‑148 (MQ=255)                                                                                                                    gACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAAcc                                  <  5:364971/148‑1 (MQ=255)                                                                                                                       ctggtcggTGACCTGGTCGTCGGCGACCATGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGt                              >  1:433575/8‑149 (MQ=39)                                                                                                                          cttcGTTACATGGTCGACGACGACATGGTCAGCGA‑GCAGGGCCTGCAGCATACCAGCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGATATGACCGCTTCCGCCACGGAACTGCTGCGCGGCATGAGCGACAAGCAGGTAgc                           <  8:270559/146‑1 (MQ=255)                                                                                                                          cTACGTGACATGGTCGCCGACGTCCATGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCTATTGTCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAgc                           <  8:272786/149‑1 (MQ=255)                                                                                                                             cgggACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCa                        <  2:179340/146‑1 (MQ=255)                                                                                                                             cGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAgcg                          <  5:439505/147‑1 (MQ=255)                                                                                                                              gTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCACCAAAGCCCCCGCGACGTCCGTGAGGCAATTCGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTCCGCGGCATCAGAGACAACCAGGAAgcc                          >  4:361579/1‑145 (MQ=255)                                                                                                                               tGACCTGGTCGTCGGCGACCATGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAgcg                          >  1:185646/1‑145 (MQ=255)                                                                                                                               tGACCTGGTCGTCGGCGACCATGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAg                            >  7:299233/1‑143 (MQ=255)                                                                                                                               tGACCTGGTCGTCGGCGACCATGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAg                            <  8:299233/143‑1 (MQ=255)                                                                                                                               tGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAgcg                          >  3:182411/1‑145 (MQ=255)                                                                                                                               tGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGa                      <  6:366026/149‑1 (MQ=255)                                                                                                                                 aCATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGAt                     <  8:375136/148‑1 (MQ=255)                                                                                                                                       tCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAg              >  4:132335/1‑149 (MQ=255)                                                                                                                                           cgacgaCCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAGGCCt          <  8:19058/149‑1 (MQ=255)                                                                                                                                           cgacgaCCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAGGCCt          >  7:255062/1‑149 (MQ=255)                                                                                                                                            gacgacCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGTTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAGGCCtt         <  6:345412/149‑1 (MQ=255)                                                                                                                                              cgacCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAGGCCTTcc       <  7:84277/149‑1 (MQ=255)                                                                                                                                              cgacCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTAGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAGGCCTTcc       <  7:348885/149‑1 (MQ=255)                                                                                                                                               ggcctggTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAActgc                                                       >  6:311964/6‑100 (MQ=255)                                                                                                                                               ggcctggTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAActgc                                                       <  5:311964/95‑1 (MQ=255)                                                                                                                                               gacCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGGATGAGCGACAACCAGGTAGCGCAGATGCGTGAGGCCTTCcg      >  5:370509/1‑149 (MQ=255)                                                                                                                                                 ccAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAGGCCTTCcgcg    <  2:188588/149‑1 (MQ=255)                                                                                                                                                   aGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAGGCCTTCCGCGaa  <  1:92227/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGA‑CCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAGGCCTTCCGCGAA  >  NC_002947/5183980‑5184274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑