Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,187,980	T→C	8.3%	L162S (TTG→TCG) ‡	PP_4567 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,187,980	0	T	C	8.3%	257.1 / 10.5	95	L162S (TTG→TCG) ‡	PP_4567	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (47/40);  new base C (4/4);  total (51/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

CTGGATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGAAGATC  >  NC_002947/5187835‑5188123                                                                                                                                                  |                                                                                                                                                         cTGGATTTCGGCTGCGCCTGTGAAACCGAACCAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTgc                                                                                                                                                        <  1:325059/148‑1 (MQ=255)  tGGATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTgcgc                                                                                                                                                      >  2:240321/1‑149 (MQ=255)   ggACTTCGGCTGCGCCTGCGAAACCGAACCAGCAGTGAAGATGTTGGCGGTCGAGGGCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGcgcc                                                                                                                                                     <  4:20887/149‑1 (MQ=255)    gATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTgcgc                                                                                                                                                      <  4:97300/147‑1 (MQ=255)      tttCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGcgccacg                                                                                                                                                  >  2:421154/1‑149 (MQ=255)           gCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGTCTGCTGGACTTCGAGGAAGGCGTGGCGCAACACGCCTTc                                                                                                                                             <  7:284829/149‑1 (MQ=255)               cgcCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATccc                                                                                                                                           <  4:145506/147‑1 (MQ=255)               cgcCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATccc                                                                                                                                           >  3:145506/1‑147 (MQ=255)                 ccTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCg                                                                                                                                         >  3:283051/1‑147 (MQ=255)                 ccTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGaa                                                                                                                                       <  5:62134/149‑1 (MQ=255)                 ccTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGaa                                                                                                                                       <  7:364715/149‑1 (MQ=255)                 ccTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGaa                                                                                                                                       <  1:421154/149‑1 (MQ=255)                  cTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCg                                                                                                                                         >  3:6810/1‑146 (MQ=255)                     tgAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGGGGATGGCGTGGCGCAACACGCCTTCCTCGAagtc                                                                                                                                    <  5:9368/148‑3 (MQ=38)                        aaCCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTTCTACCTGCTCACCCAGCGTGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTcc                                                                                                                                <  5:172623/149‑1 (MQ=255)                         aCCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCg                                                                                                                               <  8:347371/149‑1 (MQ=255)                         aCCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCg                                                                                                                               <  6:252292/149‑1 (MQ=255)                          ccGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGc                                                                                                                              >  1:102210/1‑149 (MQ=255)                                  gCAGTGAAGATGTTGGCGGTCGAGGTCACGCCGCAACTCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGATGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGcc                                                                                                              >  1:48495/1‑148 (MQ=255)                                     gTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑Ag‑g                                                                                                                 >  1:399207/1‑142 (MQ=255)                                     gTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑Ag‑g                                                                                                                 <  2:399207/142‑1 (MQ=255)                                     gTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTACTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGGGGATGGCGTGGCGCAACACGCCTTCCTCGAagtcc                                                                                                                                   <  6:226179/133‑4 (MQ=37)                                     gTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTACTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGGGGATGGCGTGGCGCAACACGCCTTCCTCGAagtcc                                                                                                                                   >  5:226179/1‑130 (MQ=37)                                        aaGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCtt                                                                                                       >  1:316099/1‑149 (MQ=255)                                         aGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTc                                                                                                      >  5:270510/1‑149 (MQ=255)                                         aGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTc                                                                                                      >  5:63292/1‑149 (MQ=255)                                         aGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTc                                                                                                      <  1:240321/149‑1 (MQ=255)                                            tGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGc                                                                                                   <  3:224916/149‑1 (MQ=255)                                             gTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGc                                                                                                   >  8:119576/1‑148 (MQ=255)                                              ttGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCa                                                                                                 <  4:283051/149‑1 (MQ=255)                                               tGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAg                                                                                                >  1:103286/1‑149 (MQ=255)                                                ggCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAg                                                                                                <  1:247318/148‑1 (MQ=255)                                                       gAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCgcgg                                                                                        <  6:341501/149‑1 (MQ=255)                                                        aGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCgcggc                                                                                       >  1:55915/1‑149 (MQ=255)                                                           tCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCggg                                                                                    <  1:106623/149‑1 (MQ=255)                                                            cccGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCggg                                                                                    <  2:366645/148‑1 (MQ=255)                                                            cccGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCggg                                                                                    >  1:366645/1‑148 (MQ=255)                                                            cccGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGc                                                                                   <  6:290463/149‑1 (MQ=255)                                                             ccgccgCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCt                                                                                  >  5:404844/1‑149 (MQ=255)                                                                ccgcAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGc                                                                               <  7:107834/149‑1 (MQ=255)                                                                 cgcAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGc                                                                               >  3:17736/1‑148 (MQ=255)                                                                      cgctcgctCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAaca                                                                         <  2:102210/149‑1 (MQ=255)                                                                          cgctcgATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAAGCCCGCCGCGCGCCTggcc                                                                             >  5:344624/1‑138 (MQ=255)                                                                          cgctcgATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAAGCCCGCCGCGCGCCTggcc                                                                             <  6:344624/141‑4 (MQ=255)                                                                           gctcgATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGaa                                                                     <  8:25818/148‑1 (MQ=255)                                                                           gctcgATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGaa                                                                     >  8:103066/1‑148 (MQ=255)                                                                           gctcgATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAAc                                                                    <  4:23264/149‑1 (MQ=255)                                                                               gATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAAcagca                                                                <  2:316099/149‑1 (MQ=255)                                                                                  aCCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGcc                                                             >  8:239826/1‑149 (MQ=255)                                                                                   cccTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGTCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGcc                                                             >  6:334814/1‑148 (MQ=255)                                                                                    ccTGCTCTACTACCTGCTCACCCCGCGCGAAGCCGGCCTGCTGCACTTCGAGGAAGGCGTGATGCGCCAAGCCATCCCCCAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGccc                                                            >  8:134989/1‑148 (MQ=255)                                                                                    ccTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGCACAGCAGccc                                                            >  8:175108/1‑148 (MQ=255)                                                                                    ccTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTAGGCCAGGCGCGCAGCGGGCTTGCGAAACACGAACAGCAGccc                                                            >  7:364493/1‑148 (MQ=255)                                                                                     cTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTa                                                          >  2:232905/1‑149 (MQ=255)                                                                                           tactacCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGa                                                    >  7:347651/1‑149 (MQ=255)                                                                                              tacCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGAt                                                   >  4:373389/1‑147 (MQ=255)                                                                                                ccTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCTGGTGGTAGATGAGCGGGCAACGCATCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTAc                                                         >  1:359613/1‑148 (MQ=255)                                                                                                    cTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCa                                           >  5:166428/1‑149 (MQ=255)                                                                                                     tCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCAt                                          >  1:252026/1‑149 (MQ=255)                                                                                                       aCCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGc                                        >  4:424126/1‑149 (MQ=255)                                                                                                        cccAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGcc                                       <  7:119576/149‑1 (MQ=255)                                                                                                         ccAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCGAAATCAGCCCCATGcc                                       >  1:150933/1‑148 (MQ=255)                                                                                                         ccAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGcc                                       >  1:231747/1‑148 (MQ=255)                                                                                                         ccAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGcc                                       >  6:239710/1‑148 (MQ=255)                                                                                                          cAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGcc                                       >  7:189735/1‑147 (MQ=255)                                                                                                          cAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGcc                                       >  3:412921/1‑147 (MQ=255)                                                                                                          cAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCca                                     <  7:236902/149‑1 (MQ=255)                                                                                                           aGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCcag                                    <  2:103286/149‑1 (MQ=255)                                                                                                             cgcgCAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCcagca                                  <  2:350528/149‑1 (MQ=255)                                                                                                               cgAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGTCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAgg                                <  5:334814/149‑1 (MQ=255)                                                                                                               cgAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAgg                                >  8:170124/1‑149 (MQ=255)                                                                                                               cgAAGACGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAgg                                <  4:17736/149‑1 (MQ=255)                                                                                                                       gCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCgg                        >  6:430596/1‑149 (MQ=255)                                                                                                                       gCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCgg                        >  5:393118/1‑149 (MQ=255)                                                                                                                        ccTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGt                       <  1:137814/149‑1 (MQ=255)                                                                                                                            ctgGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGc                     >  8:64803/1‑147 (MQ=255)                                                                                                                              ggACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAgg                 >  1:286897/1‑149 (MQ=255)                                                                                                                               gACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAgg                 <  3:424126/148‑1 (MQ=255)                                                                                                                               gACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGc                <  6:404844/149‑1 (MQ=255)                                                                                                                                aCTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCg               <  2:55915/149‑1 (MQ=255)                                                                                                                                aCTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCg               <  6:270510/149‑1 (MQ=255)                                                                                                                                aCTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCg               <  7:103066/149‑1 (MQ=255)                                                                                                                                 cTTCGGGGATGGCGTGGCGCAACACGCCTTCCTCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGcc                    >  3:203133/1‑143 (MQ=38)                                                                                                                                 cTTCGGGGATGGCGTGGCGCAACACGCCTTCCTCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGcc                    <  4:203133/143‑1 (MQ=38)                                                                                                                                  ttCGGGGATGGCGTGGCGCAACACGCCTTCCTCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGt             >  7:59245/1‑149 (MQ=38)                                                                                                                                  ttCGGGGATGGCGTGGCGCAACACGCCTTCCTCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGt             >  8:224811/1‑149 (MQ=38)                                                                                                                                  ttCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGt             >  5:129325/1‑149 (MQ=255)                                                                                                                                      aGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTAcc         >  1:24480/1‑149 (MQ=255)                                                                                                                                      aGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTAcc         <  6:393118/149‑1 (MQ=255)                                                                                                                                       ggAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTAcc         >  6:144736/1‑148 (MQ=255)                                                                                                                                        gAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCg        >  3:386555/1‑148 (MQ=255)                                                                                                                                        gAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑CGCCTACGCTACGGCCAGGCGCGCGGCTGGCTTCCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCACCAGTCTGAGCGGTGCGAGGCGGTTCCCg        >  4:312773/1‑148 (MQ=255)                                                                                                                                         aaGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGaa      <  5:144736/149‑1 (MQ=255)                                                                                                                                           ggCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCg        >  6:405515/1‑145 (MQ=255)                                                                                                                                             cGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGAAGATc  <  2:231747/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                         CTGGATTTCGGCTGCGCCTGTGAAACCGAACAAGCAGTGAAGATGTTGGCGGTCGAGGTCCCGCCGCAACGCTCGCTCGATACCCTGCTCTACTACCTGCTCACCCAGCGCGAAGCCGGCCTGCTGGACTTCGAGGAAGGCGTGTTGCGCCACGCCATCCCCGAAGAGTTCCGCT‑‑‑‑‑‑‑‑AG‑GGCCTACGCTTCGGCCAGGCGCGCGGCGGGCTTGCGAAACACGAACAGCAGCCCTACCACGATCAGCCCCATGCCCAGCAGGCTGAGCGGTGCCAGGCGGTTACCGAAGATC  >  NC_002947/5187835‑5188123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑