Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	418,296	C→G	7.1%	Q27E (CAG→GAG)	PP_0345 →	heptose kinase WapP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	418,296	0	C	G	7.1%	259.6 / 9.7	84	Q27E (CAG→GAG)	PP_0345	heptose kinase WapP
Reads supporting (aligned to +/- strand):  ref base C (39/39);  new base G (4/2);  total (43/41)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-01

CTCGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATGGCAAAA  >  NC_002947/418150‑418444                                                                                                                                                   |                                                                                                                                                      ctCGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGTGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAg                                                                                                                                                     >  1:365860/1‑149 (MQ=255) ctCGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAg                                                                                                                                                     >  7:57273/1‑149 (MQ=255)   cGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAggg                                                                                                                                                   <  2:419751/149‑1 (MQ=255)    gCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAgg                                                                                                                                                    <  1:140189/147‑1 (MQ=255)    gCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGc                                                                                                                                                  >  1:226663/1‑149 (MQ=255)    gCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGATCGCCCTCCAgg                                                                                                                                                    <  3:416748/147‑1 (MQ=255)      ctggctggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCg                                                                                                                                                 >  6:378826/1‑148 (MQ=255)         gctggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTGGAGGGCGaa                                                                                                                                               <  6:24743/147‑1 (MQ=255)         gctggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACg                                                                                                                                             >  1:218574/1‑149 (MQ=255)         gctggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACg                                                                                                                                             >  5:248873/1‑149 (MQ=255)         gcgggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAAc                                                                                                                                              <  6:335425/145‑1 (MQ=255)          ctggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACgg                                                                                                                                            >  8:70312/1‑149 (MQ=255)           tggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGc                                                                                                                                           >  4:267587/1‑149 (MQ=255)           tggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGAGTTCGCCCTCCAGGGCGAACGGc                                                                                                                                           >  8:404694/1‑149 (MQ=255)             gAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAGCGGCTg                                                                                                                                         >  4:305485/1‑149 (MQ=255)             gAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGCGTTCGCCCTGGAGGGCGAAcaccgc                                                                                                                                         <  3:414972/149‑6 (MQ=255)              aGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTga                                                                                                                                        <  4:401331/149‑1 (MQ=255)              aGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTga                                                                                                                                        <  4:394973/149‑1 (MQ=255)                  cAAGGCCCAGAAACTCTACGATAGAAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGACGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgacccg                                                                                                                                    >  6:210939/1‑149 (MQ=255)                       cccAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgacccgcgacc                                                                                                                               <  2:136916/149‑1 (MQ=255)                       cccAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgacccgcgacc                                                                                                                               >  8:304374/1‑149 (MQ=255)                        ccAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgacccgcgaccc                                                                                                                              >  8:351119/1‑149 (MQ=255)                        ccAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgacccgcgaccc                                                                                                                              >  2:308700/1‑149 (MQ=255)                          aGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgacccgcgacccgc                                                                                                                            >  5:25900/1‑149 (MQ=255)                           gAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCt                                                                                                                           >  6:343926/1‑149 (MQ=255)                             aaCTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCACGGCAAACGGCTGACCCGCGACCCGCTCa                                                                                                                         <  1:245797/149‑1 (MQ=255)                                tctACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGt                                                                                                                       >  8:303235/1‑148 (MQ=255)                                tctACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGt                                                                                                                       >  6:64037/1‑148 (MQ=255)                                  tccGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACGTCGGCAGCCTCGACGCGGTGTTCGCGCTCCAGGGCGAACGGCTGACCCGAGACGCGCGCAGTg                                                                                                                      >  6:334866/3‑147 (MQ=255)                                  tACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTg                                                                                                                      >  5:348340/1‑147 (MQ=255)                                    cGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGaa                                                                                                                    <  6:248873/147‑1 (MQ=255)                                    cGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAg                                                                                                                   <  7:70312/148‑1 (MQ=255)                                     gATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTg                                                                                                                 <  4:227760/149‑1 (MQ=255)                                     gATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTg                                                                                                                 >  7:268720/1‑149 (MQ=255)                                         gcaagcaaCGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTa                                                                                                              <  8:268720/148‑1 (MQ=255)                                          caagcaaCGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACg                                                                                                            >  8:82205/1‑149 (MQ=255)                                           aagcaaCGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgacccg                                                                                                                                    >  1:5065/1‑124 (MQ=255)                                           aagcaaCGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgacccg                                                                                                                                    <  2:5065/124‑1 (MQ=255)                                                    tATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGt                                                                                                  >  5:29757/1‑149 (MQ=255)                                                      tGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTg                                                                                                 <  7:351119/148‑1 (MQ=255)                                                        gggATGCACTCTGATGGCGAGTTGGACACTTGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACg                                                                                              >  8:81335/1‑149 (MQ=255)                                                        gggATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACg                                                                                              <  5:343926/149‑1 (MQ=255)                                                            tGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGAt                                                                                          <  6:29757/149‑1 (MQ=255)                                                                ctctGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAac                                                                                      <  7:134040/149‑1 (MQ=255)                                                                ctctGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAac                                                                                      <  8:57273/149‑1 (MQ=255)                                                                        gCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGt                                                                              >  8:127382/1‑149 (MQ=255)                                                                              tGGACACTGGCGCCGGGGTACTAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTGCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGTATCAACTACTACGTCAAg‑c                                                                        <  2:218574/148‑1 (MQ=255)                                                                                gACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCt                                                                     <  7:304374/149‑1 (MQ=255)                                                                                 acacTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTa                                                                    >  3:54246/1‑149 (MQ=255)                                                                                  cacTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTac                                                                   <  5:426322/149‑1 (MQ=255)                                                                                        cgcCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCggg                                                             <  3:96257/149‑1 (MQ=255)                                                                                        cgcAGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGTCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCggg                                                             <  5:210939/149‑1 (MQ=255)                                                                                         gcCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCgggg                                                            <  5:64037/149‑1 (MQ=255)                                                                                            ggggTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAAGGCGAACGGCTGACCCGCGACCCGCTCAGTTAAGTGGTACGCATCTAGCGTGACGGGATAAACTACTACGTCAAG‑CCCTACACCGGCGCCg                                                         >  8:152102/1‑149 (MQ=255)                                                                                             gggTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCgg                                                        <  6:348340/149‑1 (MQ=255)                                                                                                   gAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGGCAAGca                                                  >  3:212960/1‑149 (MQ=255)                                                                                                   gAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGGCAAGca                                                  >  6:232251/1‑149 (MQ=255)                                                                                                    aaCACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGGCAAGcac                                                 <  4:54246/149‑1 (MQ=255)                                                                                                      cacCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGGCAAGCACAt                                               <  4:212960/149‑1 (MQ=255)                                                                                                      cacCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGACAAGCACAt                                               <  2:226663/149‑1 (MQ=255)                                                                                                             cggcggACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAATGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGGCAAGCACATGCGCCgg                                        <  4:75913/149‑1 (MQ=255)                                                                                                                     ttCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTggg                                >  1:57046/1‑149 (MQ=255)                                                                                                                         gCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGggcgg                            <  5:378826/149‑1 (MQ=255)                                                                                                                         gCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGggcgg                            >  2:163551/1‑149 (MQ=255)                                                                                                                          cAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGggcggc                           <  7:152102/149‑1 (MQ=255)                                                                                                                           aGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGggcggc                           <  1:308700/149‑1 (MQ=255)                                                                                                                             ccTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCAc                        <  7:82205/149‑1 (MQ=255)                                                                                                                              cTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACg                       >  8:46008/1‑149 (MQ=255)                                                                                                                               tCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACg                       >  8:10878/1‑148 (MQ=255)                                                                                                                                cGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCa                     >  7:281595/1‑149 (MQ=255)                                                                                                                                 gACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATAGACCGTGACGGGATCAACTACTACGTCAAC‑CGCTACACCGGGGCCCGCAAGCACAAGCGCCCGTACCTGGGGCGGCCACGCa                     >  6:274143/1‑148 (MQ=255)                                                                                                                                    gcgGGGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCaa                 <  5:129235/149‑1 (MQ=255)                                                                                                                                     cgGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAg                <  2:57046/149‑1 (MQ=255)                                                                                                                                      ggTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAgg               <  2:365860/149‑1 (MQ=255)                                                                                                                                      ggTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAgg               >  7:171191/1‑149 (MQ=255)                                                                                                                                      ggTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGCTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAgg               <  3:305485/149‑1 (MQ=255)                                                                                                                                         gtTCGCCCTGGAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGcc             >  4:414875/1‑148 (MQ=255)                                                                                                                                         gtTCGCCCTGGAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGcc             >  6:353881/1‑148 (MQ=255)                                                                                                                                         gtTCGCCCTGGAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGcc             >  3:85001/1‑148 (MQ=255)                                                                                                                                          ttCGCCCTACAGGGCGAACGGCTGACCCGCTACCCGCTCAGTGAAGTGGTAAGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACAACGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCGACGCATCAAGGACg            >  8:197446/1‑148 (MQ=255)                                                                                                                                            cGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAAt         >  2:419967/1‑149 (MQ=255)                                                                                                                                             gCCCTGGAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATg        >  4:264147/1‑149 (MQ=255)                                                                                                                                             gCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAAt         <  7:127382/148‑1 (MQ=255)                                                                                                                                                cTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTTACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATGGCa     <  7:404694/149‑1 (MQ=255)                                                                                                                                                   cAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATGGCaaaa  <  5:274143/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                      CTCGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAG‑CGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATGGCAAAA  >  NC_002947/418150‑418444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑