Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	432,365	G→T	6.9%	intergenic (‑52/‑41)	PP_0354 ← / → PP_0355	CBS domain‑containing protein/two‑component system response regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	432,365	0	G	T	6.9%	209.0 / 4.2	70	intergenic (‑52/‑41)	PP_0354/PP_0355	CBS domain‑containing protein/two‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base G (33/32);  new base T (3/2);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

GAACGGCGGAAAGCGCTGCAGGAACTGCAATGTGCCCTGGATGTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCGGAATTTTTTT  >  NC_002947/432218‑432513                                                                                                                                                      |                                                                                                                                                     gAACGGCGGAAAGCGCTGCAGGAACTGCAATGTGCCCTGGATGTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCg                                                                                                                                                      <  7:220302/148‑1 (MQ=255) gAACGGCGGAAAGCGCTGCAGGAACTGCAATGTGCCCTGGATGTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCg                                                                                                                                                      <  5:188960/148‑1 (MQ=255)  aaCGGCGGAAAGCGCTGCAGGAACTGCAATGTGCCCTGGATGTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGc                                                                                                                                                    >  3:160646/1‑149 (MQ=255)    cggcggAAAGCGCTGCAGGAACTGCAATGTGCCCTGGATGTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCa                                                                                                                                                  <  5:353445/149‑1 (MQ=255)      gcggAAAGCGCTGCAGGAACTGCAATGTGCCCTGGATGTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCAtg                                                                                                                                                <  5:421805/149‑1 (MQ=255)      gcggAAAGCGCTGCAGGAACTGCAATGTGCCCTGGATGTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCAtg                                                                                                                                                <  4:244468/149‑1 (MQ=255)       cggATAGCGCTGCAGGAACTGCAATGTGCCCTTGATGTTTTGCAGTTCCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCAt                                                                                                                                                 <  2:150008/147‑1 (MQ=255)       cggAAAGCGCTGCAGGAACTGCAATGTGCCCTGGATGTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCAtgt                                                                                                                                               <  6:302739/149‑1 (MQ=255)        ggAAAGCGCTGCAGGAACTGCAATGTGCCCTGGATGTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCAtgtt                                                                                                                                              <  5:160282/149‑1 (MQ=255)         gAAAGCGCTGCAGGAACTGCAATGTGCCCTGGATGTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCAtgttg                                                                                                                                             <  8:257623/149‑1 (MQ=255)         gAAAGCGCTGCAGGAACTGCAATGTGCCCTGGATGTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCAtgttg                                                                                                                                             >  8:253337/1‑149 (MQ=255)                cTGCAGGAACTGCAATGTGCCCTGGATGTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTg                                                                                                                                      <  7:47114/149‑1 (MQ=255)                   cAGGAACTGCAATGTGCCCTGGATGTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCt                                                                                                                                   >  8:76190/1‑149 (MQ=255)                      gAACTGCAATGTGCCCTGGATGTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCTGCCATGTTGTTCTCTGCCTCCa                                                                                                                                >  5:362629/1‑149 (MQ=255)                      gAACTGCAATGTGCCCTGGATGTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCTGCCATGTTGTTCTCTGCCTCCa                                                                                                                                >  3:69195/1‑149 (MQ=255)                       aaCTGCAATGTGCCCTGGATGTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCAt                                                                                                                               <  4:1279/149‑1 (MQ=255)                           gCAATGTGCCCTGGATGTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTc                                                                                                                           >  5:37229/1‑149 (MQ=255)                            cAATGTGCCCTGGATGTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCTGCCATGTTGTTCTCTGCCTCCATGGTCg                                                                                                                          <  6:362629/149‑1 (MQ=255)                            cAATGTGCCCTGGATGTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCTGCCATGTTGTTCTCTGCCTCCATGGTCg                                                                                                                          <  4:69195/149‑1 (MQ=255)                            cAATGTGCCCTGGATGTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTc                                                                                                                           <  6:43293/148‑1 (MQ=255)                            cAATGTGCCCTGGATGTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCg                                                                                                                          >  7:90626/1‑149 (MQ=255)                              aTGTGCCCTGGATGTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGc                                                                                                                        >  4:340755/1‑149 (MQ=255)                                 tgCCCTGGATGTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTg                                                                                                                     <  4:160646/149‑1 (MQ=255)                                 tgCCCTGGATGTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTg                                                                                                                     >  8:81234/1‑149 (MQ=255)                                      tGGATGTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTgcggcg                                                                                                                >  5:95832/1‑149 (MQ=255)                                       ggATCTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCAATATATAGGTACCGGCCATGATGTTCTCTGCCTGCATGTTCGGCTTGcggcgg                                                                                                               >  5:263624/1‑149 (MQ=255)                                        gATGTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCggg                                                                                                              <  3:340755/149‑1 (MQ=255)                                         aTGTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCgggg                                                                                                             >  7:268350/1‑149 (MQ=255)                                            ttttGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGggtg                                                                                                           <  6:95832/148‑1 (MQ=255)                                              ttGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGggtggt                                                                                                         <  4:109987/148‑1 (MQ=255)                                                gCAGTACCGCTGTCTTGCCCTCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGc                                                                                                       <  7:76190/148‑1 (MQ=255)                                                 cAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGcc                                                                                                      >  7:326974/1‑148 (MQ=255)                                                  aGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCTGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCa                                                                                                    >  8:312929/1‑149 (MQ=255)                                                       cGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGa                                                                                               >  4:190556/1‑149 (MQ=255)                                                       cGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGa                                                                                               <  1:435346/149‑1 (MQ=255)                                                       cGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGATCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGa                                                                                               >  1:175546/1‑149 (MQ=255)                                                         cTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACg                                                                                             >  5:323395/1‑149 (MQ=255)                                                            tCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTaa                                                                                          <  2:278706/149‑1 (MQ=255)                                                              ttGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGt                                                                                        >  3:405247/1‑149 (MQ=255)                                                                  ccGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTAt                                                                                    >  2:31316/1‑149 (MQ=255)                                                                   cGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATg                                                                                   <  7:81234/149‑1 (MQ=255)                                                                      cTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAgc                                                                                >  3:161360/1‑149 (MQ=255)                                                                          cTGTAGGCTTCCTTTTTGCTCATGAATCTGACCGTATCGCTATGCAG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGccc                                                                            <  8:106325/149‑1 (MQ=255)                                                                           tGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGAGGGGTGGTGCACATTGGACGTAAGTCTATGAGCGCCCt                                                                           >  3:276781/1‑149 (MQ=255)                                                                           tGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCAATGGTCGGCTTGCCGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCt                                                                           >  5:360466/1‑149 (MQ=255)                                                                                 cGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCa                                                                      >  1:244146/1‑148 (MQ=255)                                                                                  gTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCaaa                                                                    <  4:405247/149‑1 (MQ=255)                                                                                  gTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCaaa                                                                    <  8:326974/149‑1 (MQ=255)                                                                                   tCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCGGTACCTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCa                                                                      >  8:293255/1‑148 (MQ=255)                                                                                   tCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCa                                                                      >  7:288467/1‑146 (MQ=255)                                                                                     cTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGa                                                                  <  5:135103/148‑1 (MQ=255)                                                                                     cTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGAc                                                                 >  2:379805/1‑149 (MQ=255)                                                                                             tCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCaaa                                                                    <  4:383322/138‑1 (MQ=255)                                                                                             tCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCaaa                                                                    >  3:383322/1‑138 (MQ=255)                                                                                               aTGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAg                                                       >  5:19930/1‑149 (MQ=255)                                                                                                      tGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAAc                                                >  2:378647/1‑149 (MQ=255)                                                                                                        aCCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACgg                                              <  6:323395/149‑1 (MQ=255)                                                                                                         ccGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACggg                                             <  2:244146/149‑1 (MQ=255)                                                                                                          cGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAg                                                                   >  4:381792/1‑126 (MQ=255)                                                                                                          cGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAg                                                                   <  3:381792/126‑1 (MQ=255)                                                                                                                       cG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATg                               >  4:335715/1‑149 (MQ=255)                                                                                                                           aaCTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCg                             <  3:335715/149‑1 (MQ=255)                                                                                                                             ctaattatatCTATATATTGGTTCGGGCCATGTAGTTCTCTCCCTCCATGGTCTGCTTGCGGCGGGGTGGTGCGCTTTGGACGTAAGTCTTTGAGCGCCCTTGTCTAAGACGCGACGAAACGCGAAACGGGTTACGCGAGTATTGCGGt                           <  6:263624/144‑1 (MQ=255)                                                                                                                              tatatataTCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTc                          <  7:253337/149‑1 (MQ=255)                                                                                                                                tatatatCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTcc                         >  8:120625/1‑148 (MQ=255)                                                                                                                                    tatCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAAt                     <  1:379805/148‑1 (MQ=255)                                                                                                                                    tatCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATg                    <  5:9308/149‑1 (MQ=255)                                                                                                                                     atCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGc                   >  6:168211/1‑149 (MQ=255)                                                                                                                                         atatataGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTg                 >  5:412908/1‑147 (MQ=255)                                                                                                                                            tataGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCg            <  7:293255/149‑1 (MQ=255)                                                                                                                                              taGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCgg           >  2:352352/1‑148 (MQ=255)                                                                                                                                                    ccGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCGGAAttttt    >  5:151740/1‑149 (MQ=255)                                                                                                                                                      ggCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCGGAAttttttt  <  4:161360/149‑1 (MQ=255)                                                                                                                                                      ggCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCGGAAttttttt  <  1:352352/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                     GAACGGCGGAAAGCGCTGCAGGAACTGCAATGTGCCCTGGATGTTTTGCAGTACCGCTGTCTTGCCCGCCTGGCTGTAGGCGTCCTTTTTGCTCATGAAACTGACCGTATCGCTATGCCG‑‑AACTATATATATCTATATATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCGGAATTTTTTT  >  NC_002947/432218‑432513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑