Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,305,662 C→T 5.5% L530L (CTG→CTA) ‡ recC ← multifunctional ATP‑dependent DNA helicase/dsDNA/ssDNA exonuclease/ssDNA endonuclease recombinase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,305,6620CT 5.5% 280.9 / 3.4 91L530L (CTG→CTA) ‡recCmultifunctional ATP‑dependent DNA helicase/dsDNA/ssDNA exonuclease/ssDNA endonuclease recombinase subunit
Reads supporting (aligned to +/- strand):  ref base C (49/37);  new base T (3/2);  total (52/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01

CGGGCCGATCAGTGCCGCGTCCAGGCCACCGATTTCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTCGCTGACCGC  >  NC_002947/5305515‑5305808
                                                                                                                                                    |                                                                                                                                                  
cGGGCCGATCAGTGCCGCGTCCAGGCCACCGATTTCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCa                                                                                                                                                   <  1:243215/149‑1 (MQ=255)
    ccGATCAGTGCCGCGTCCAGGCCACCGATTTCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCt                                                                                                                                               >  5:770/1‑149 (MQ=255)
        tCAGTGCCGCGTCCAGGCCACCGATTTCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGcc                                                                                                                                           <  1:335091/149‑1 (MQ=255)
           gTGCCGCGTCCAGGCCACCGATTTCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGc                                                                                                                                        >  3:190174/1‑149 (MQ=255)
              ccGCGTCCAGGCCACCGATTTCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTgc                                                                                                                                     <  4:190174/149‑1 (MQ=255)
                gcgTCCAGGCCACCGATTTCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTgcgc                                                                                                                                   <  3:310554/149‑1 (MQ=255)
                gcgTCCAGGCCACCGATTTCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTgcgc                                                                                                                                   <  7:123455/149‑1 (MQ=255)
                   tCCAGGCCACCGATTTCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCgcggc                                                                                                                                <  8:181284/149‑1 (MQ=255)
                      aGGCCACCGATTTCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCTACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGt                                                                                                                              >  3:400217/1‑148 (MQ=255)
                      aGGCCACCGATTTCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCGAGC‑CTGGGCTTGCCCGcc                                                                                                                                       <  3:358107/139‑2 (MQ=255)
                      aGGCCACCGATTTCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCGAGC‑CTGGGCTTGCCCGcc                                                                                                                                       >  4:358107/1‑138 (MQ=255)
                      aGGCCACCGATTTCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTc                                                                                                                             <  4:179070/149‑1 (MQ=255)
                           aCCGATTTCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGc                                                                                                                        <  3:23134/149‑1 (MQ=255)
                           aCCGATTTCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGc                                                                                                                        <  7:74590/149‑1 (MQ=255)
                           aCCGATTTCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGc                                                                                                                        >  5:438156/1‑149 (MQ=255)
                                  tCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAAc                                                                                                                  <  5:177403/148‑1 (MQ=255)
                                  tCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAAc                                                                                                                  <  4:368677/148‑1 (MQ=255)
                                      cGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCgcggc                                                                                                                                >  8:258457/1‑130 (MQ=255)
                                      cGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCgcggc                                                                                                                                <  7:258457/130‑1 (MQ=255)
                                       gTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGc                                                                                                            >  6:403919/1‑149 (MQ=255)
                                        tAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGCGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGc                                                                                                            >  6:156686/1‑148 (MQ=255)
                                          gggTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCa                                                                                                          <  1:20761/148‑1 (MQ=255)
                                            gTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAgcg                                                                                                       >  1:281229/1‑149 (MQ=255)
                                                aaTGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCt                                                                                                   >  1:14007/1‑149 (MQ=255)
                                                    ccATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGa                                                                                                <  4:154250/148‑1 (MQ=255)
                                                    agttCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGa                                                                                                <  1:374935/145‑1 (MQ=255)
                                                      aTCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATcc                                                                                             >  3:302703/1‑149 (MQ=255)
                                                      aTCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATcc                                                                                             >  4:415333/1‑149 (MQ=255)
                                                             gCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTg                                                                                      >  5:73632/1‑149 (MQ=255)
                                                             gCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTg                                                                                      >  3:375713/1‑149 (MQ=255)
                                                              ccTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGc                                                                                     <  5:383554/149‑1 (MQ=255)
                                                               cTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTg                                                                                      >  4:351872/1‑147 (MQ=255)
                                                               cTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCa                                                                                    >  4:405251/1‑149 (MQ=255)
                                                                  acccacccCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGa                                                                                                >  2:207763/1‑134 (MQ=255)
                                                                  acccacccCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGa                                                                                                <  1:207763/134‑1 (MQ=255)
                                                                      acccCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGAGGTGCAGCGTggg                                                                             >  7:397922/1‑149 (MQ=255)
                                                                       cccccACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAATGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGc                                                                            <  3:295009/149‑1 (MQ=255)
                                                                       cccccACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGc                                                                            <  2:281229/149‑1 (MQ=255)
                                                                        ccccACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTggg                                                                             >  5:136441/1‑147 (MQ=255)
                                                                         cccACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAg                                                                          >  1:248204/1‑149 (MQ=255)
                                                                           cACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCTAGGCACCAACGGATGCCCGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGt                                                                        >  4:188028/1‑149 (MQ=255)
                                                                            aCGGCATAACCCATCATCATGCGTCGCACGCCGAAACGCCAACTCTTCTCTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCCGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTc                                                                       <  8:289620/149‑1 (MQ=255)
                                                                              ggCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCATCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGt                                                                     >  7:141864/1‑149 (MQ=255)
                                                                                cATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGcc                                                                                                     <  2:99816/115‑1 (MQ=255)
                                                                                cATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGcc                                                                                                     >  1:99816/1‑115 (MQ=255)
                                                                                cATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGttt                                                                   >  6:167370/1‑149 (MQ=255)
                                                                                cATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGttt                                                                   <  8:76442/149‑1 (MQ=255)
                                                                                 aTAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGtttt                                                                  <  2:22681/149‑1 (MQ=255)
                                                                                     cccAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTcgcg                                                              <  4:375713/149‑1 (MQ=255)
                                                                                      ccAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTcgcg                                                              >  2:258764/1‑148 (MQ=255)
                                                                                       cAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGa                                                            >  1:269199/1‑149 (MQ=255)
                                                                                          cAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGc                                                         <  6:136441/149‑1 (MQ=255)
                                                                                          cAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGc                                                         >  1:71055/1‑149 (MQ=255)
                                                                                           aGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGcc                                                        <  3:405251/149‑1 (MQ=255)
                                                                                            gCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCGCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGcc                                                        >  1:114518/1‑149 (MQ=255)
                                                                                                gcggcgCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGCCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGc                                                   >  6:7446/1‑149 (MQ=255)
                                                                                                 cggcgCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGc                                                                            >  8:167455/1‑123 (MQ=255)
                                                                                                     gcAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGgcg                                              >  2:14712/1‑149 (MQ=255)
                                                                                                       aGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAATGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGcgcg                                            >  5:291227/1‑149 (MQ=255)
                                                                                                       aGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGcgcg                                            <  2:269199/149‑1 (MQ=255)
                                                                                                         gCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGa                                          >  5:366101/1‑149 (MQ=255)
                                                                                                          ccGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCTGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCgg                                                 >  4:132800/1‑141 (MQ=255)
                                                                                                          ccGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCTGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCgg                                                 <  3:132800/141‑1 (MQ=255)
                                                                                                           cGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACt                                        <  2:71055/149‑1 (MQ=255)
                                                                                                             aaaCGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGc                                      <  1:14712/149‑1 (MQ=255)
                                                                                                                cGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCATCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCgg                                   <  8:141864/149‑1 (MQ=255)
                                                                                                                 gCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCgg                                   >  8:209997/1‑148 (MQ=255)
                                                                                                                   cAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCAc                                >  4:222358/1‑149 (MQ=255)
                                                                                                                   cAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCAc                                >  8:387157/1‑149 (MQ=255)
                                                                                                                    aaCTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACg                               >  5:49821/1‑149 (MQ=255)
                                                                                                                      ctgttctgttCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTc                             <  6:49821/149‑1 (MQ=255)
                                                                                                                         ttctgttcGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCcag                          <  5:167370/149‑1 (MQ=255)
                                                                                                                         ttctgttcGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCcag                          <  7:209997/149‑1 (MQ=255)
                                                                                                                          tctgttcGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCcag                          >  6:342643/1‑148 (MQ=255)
                                                                                                                           ctgttcGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCcag                          >  6:327120/1‑147 (MQ=255)
                                                                                                                            tgttcGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCcagcag                       >  8:80754/1‑149 (MQ=255)
                                                                                                                              ttcGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAgg                      <  6:366101/148‑1 (MQ=255)
                                                                                                                                  aGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAg                 <  3:222358/149‑1 (MQ=255)
                                                                                                                                     ccGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCAc              >  5:17878/1‑149 (MQ=255)
                                                                                                                                     ccGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCAc              >  2:316428/1‑149 (MQ=255)
                                                                                                                                        gcggGCGAGC‑CTGGGCTTGCCCGCTGCGCGGCGTCAAGGCCCCAACGGGTGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGgcgc                                             >  7:356976/1‑115 (MQ=255)
                                                                                                                                        gcggGCGAGC‑CTGGGCTTGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGgcgc                                             <  8:356976/115‑1 (MQ=255)
                                                                                                                                        gcggGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTc           >  4:294212/1‑149 (MQ=255)
                                                                                                                                        gcggGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTc           >  6:198734/1‑149 (MQ=255)
                                                                                                                                         cggGCGAGC‑CTGGGCTTGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTCg          >  5:72178/1‑149 (MQ=255)
                                                                                                                                             cAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTCGCTGa      >  7:272506/1‑149 (MQ=255)
                                                                                                                                             cAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTCGCTGa      >  2:264679/1‑149 (MQ=255)
                                                                                                                                             cAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTCGCTGa      <  6:73632/149‑1 (MQ=255)
                                                                                                                                             cAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGAGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTCGCTGa      >  5:310040/1‑149 (MQ=255)
                                                                                                                                             cAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCAAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCCGCACGTCCACCAGGTCGAGCACTACGCtca      >  8:185251/1‑147 (MQ=255)
                                                                                                                                             cAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGAACCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTCGCTGa      >  7:269899/1‑149 (MQ=255)
                                                                                                                                                 c‑ccAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTCGCTGACcgc  <  2:14007/149‑1 (MQ=255)
                                                                                                                                                 c‑ccAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTCGCTGACcgc  <  5:327120/149‑1 (MQ=255)
                                                                                                                                                 c‑ccAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTCGCTGACcg   <  4:355599/148‑1 (MQ=255)
                                                                                                                                                 c‑ccAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTCGCTGACcg   >  3:355599/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGGGCCGATCAGTGCCGCGTCCAGGCCACCGATTTCATCGTAGGGTTCAATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGC‑CCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTCGCTGACCGC  >  NC_002947/5305515‑5305808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑