Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 432,993 A→T 6.7% intergenic (+18/+29) PP_0355 → / ← glcB two‑component system response regulator/malate synthase G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947432,9930AT 6.7% 226.6 / 5.0 75intergenic (+18/+29)PP_0355/glcBtwo‑component system response regulator/malate synthase G
Reads supporting (aligned to +/- strand):  ref base A (40/30);  new base T (3/2);  total (43/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

GTGTCGGGCGACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTGGAACGCCACGTT  >  NC_002947/432847‑433141
                                                                                                                                                  |                                                                                                                                                    
gtgtCGGGCGACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTaa                                                                                                                                                     >  6:224050/1‑148 (MQ=255)
 tgtCGGGCGACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTaaaa                                                                                                                                                   >  7:343539/1‑149 (MQ=255)
 tgtCGGGCGACACCGATGTGGAAGAGGCAGTGGAAGTGATTCACCTGGTGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTaaaa                                                                                                                                                   <  2:292851/149‑1 (MQ=255)
  gtCGGGCGACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAAc                                                                                                                                                  >  2:137751/1‑149 (MQ=255)
     gggCGACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGt                                                                                                                                               <  6:282761/149‑1 (MQ=255)
        cGACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTccc                                                                                                                                            >  5:405887/1‑149 (MQ=255)
        cGACACCGATGTGGAAGAGGCAGTGCACGTGATGCACCTGGGGGAGCTGGACTTCCTGCTCAACCCGGTGGATCTGGGCAAGCTGGTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTccc                                                                                                                                            <  2:163979/149‑1 (MQ=255)
         gACACCGATGTGGAAGCGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTcccc                                                                                                                                           <  8:356858/149‑1 (MQ=255)
          acacCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTTTTACGGTcc                                                                                                                                             <  1:403028/146‑1 (MQ=255)
          acacCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTTTTACGGTcc                                                                                                                                             >  2:403028/1‑146 (MQ=255)
              cGATTTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGGCCCCCTgg                                                                                                                                       >  5:209439/1‑148 (MQ=255)
              cGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTTTTACGGTcc                                                                                                                                             >  5:105285/1‑142 (MQ=255)
              cGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTTTTACGGTcc                                                                                                                                             <  6:105285/142‑1 (MQ=255)
              cGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTgg                                                                                                                                       >  6:103577/1‑148 (MQ=255)
               gATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGgca                                                                                                                                     <  4:339781/149‑1 (MQ=255)
                     gAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATAATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGa                                                                                                                                <  3:417500/148‑1 (MQ=255)
                       agagGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGCCCGTAAAACGGTCCCCCTGGCAGCAGAtgc                                                                                                                             >  7:120480/1‑149 (MQ=255)
                         agGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAATCTTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGAtgctg                                                                                                                           <  1:113973/149‑1 (MQ=255)
                         agGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGAtgctg                                                                                                                           <  5:177535/149‑1 (MQ=255)
                                 gctgtgATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAAc                                                                                                                   <  8:341654/147‑1 (MQ=255)
                                   tgtgATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGCGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAAccc                                                                                                                 <  1:306974/149‑1 (MQ=255)
                                      gATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGt                                                                                                               >  5:24209/1‑148 (MQ=255)
                                             cTGGGGGAGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGATGTTGGAGCTGGTGAACAAAGTACTGAAAATTCGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACACGTTACGCGcc                                                                                                       >  5:309718/1‑149 (MQ=255)
                                                ggggTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCtt                                                                                                     <  6:405887/148‑1 (MQ=255)
                                                ggggTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCtt                                                                                                     >  4:150721/1‑148 (MQ=255)
                                                          aCTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCgcgg                                                                                           <  3:150721/148‑1 (MQ=255)
                                                           cTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCgcggc                                                                                          >  3:163549/1‑148 (MQ=255)
                                                                 gCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGAcggcg                                                                                   >  2:350661/1‑149 (MQ=255)
                                                                  cTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGAcggcgg                                                                                  >  6:77752/1‑149 (MQ=255)
                                                                   tCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAAccc                                                                                                                 >  5:216491/1‑117 (MQ=255)
                                                                   tCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAAccc                                                                                                                 <  6:216491/117‑1 (MQ=255)
                                                                     aaGCCGGTGGATCTGGTCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGTGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTg                                                                                <  5:103577/148‑1 (MQ=255)
                                                                     aaGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTg                                                                                >  2:400280/1‑148 (MQ=255)
                                                                     aaGCCGGTGGATCTGGGAAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGc                                                                               <  3:358986/149‑1 (MQ=255)
                                                                           gTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTAcc                                                                         >  1:158671/1‑149 (MQ=255)
                                                                           gTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTAcc                                                                         <  5:224050/149‑1 (MQ=255)
                                                                             ggATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCgg                                                                       >  3:281503/1‑149 (MQ=255)
                                                                                tCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTc                                                                    >  3:410377/1‑149 (MQ=255)
                                                                                tCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTc                                                                    >  5:24281/1‑149 (MQ=255)
                                                                                    ggCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCg                                                                   <  4:410377/146‑1 (MQ=255)
                                                                                       aaGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATaa                                                             >  8:346492/1‑149 (MQ=255)
                                                                                        aGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGCCGGTGCAGTACCGGCTCGGTATAAc                                                            >  5:234003/1‑149 (MQ=255)
                                                                                          cTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTcgc                                                                                             <  6:37221/114‑1 (MQ=255)
                                                                                          cTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTcgc                                                                                             >  5:37221/1‑114 (MQ=255)
                                                                                           tGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGt                                                         >  1:129493/1‑149 (MQ=255)
                                                                                            gTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGtt                                                        >  1:406738/1‑149 (MQ=255)
                                                                                             ttGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTc                                                       >  4:102299/1‑149 (MQ=255)
                                                                                                      gTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGt                                                                 >  3:322620/1‑130 (MQ=255)
                                                                                                      gTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGt                                                                 <  4:322620/130‑1 (MQ=255)
                                                                                                       tGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGttgcttgc                                             <  1:400280/149‑1 (MQ=255)
                                                                                                          aCAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCa                                           <  8:343539/148‑1 (MQ=255)
                                                                                                           cAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCAcc                                         >  8:130657/1‑149 (MQ=255)
                                                                                                            aaaGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCt                                        >  2:113806/1‑149 (MQ=255)
                                                                                                              aGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTc                                      <  2:170077/149‑1 (MQ=255)
                                                                                                               gAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTcc                                     <  6:234003/149‑1 (MQ=255)
                                                                                                                 aCTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCAc                                   <  4:163549/149‑1 (MQ=255)
                                                                                                                   tGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTa                                 >  2:261689/1‑149 (MQ=255)
                                                                                                                   tGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTa                                 >  5:430348/1‑149 (MQ=255)
                                                                                                                   tGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTa                                 >  1:275854/1‑149 (MQ=255)
                                                                                                                     aacaTTGGATGATGCGCCAGGGGGACCGTACAACGGGCCCCCTGGCAGCAGATTCTGCTTACAACCCGTTTCGCGCGTTGAACTCGCGGCGACGGCGGGGCAGTCCCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACtcc                                <  1:137751/145‑3 (MQ=255)
                                                                                                                         ttGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGtt                           <  3:102299/149‑1 (MQ=255)
                                                                                                                             atgatgCGCCAGGGGGACCGTAAAACGGGCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCcac                       <  1:127995/149‑1 (MQ=255)
                                                                                                                                atgCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTg                                                                                                    <  3:364519/69‑1 (MQ=255)
                                                                                                                                atgCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTg                                                                                                    <  3:370551/69‑1 (MQ=255)
                                                                                                                                atgCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTg                                                                                                    >  4:364519/1‑69 (MQ=255)
                                                                                                                                atgCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTg                                                                                                    >  4:370551/1‑69 (MQ=255)
                                                                                                                                atgCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAgc                    >  5:327130/1‑149 (MQ=255)
                                                                                                                                    gcCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAgccgcc                >  4:6337/1‑149 (MQ=255)
                                                                                                                                    gcCAGGGGGACCGTAAAACGGTCACCCTGGCAGCAGATTCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGCCGGCGGTGCTGTACCGGCTCGGTATAACCGTTAGGTTGCTTGCCACCTTCCACTACCAGATCCACAgcccca                >  3:253182/1‑146 (MQ=255)
                                                                                                                                       aGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGCATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTgg             <  1:113806/149‑1 (MQ=255)
                                                                                                                                       aGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGAAGGCGGTGAAGTACCGGCTCGGTATAACCGTTAGGTTGCTTGCCACCTTCCACGACCAGTTCCACAGCCGCCTg              >  8:170581/1‑148 (MQ=255)
                                                                                                                                        gggggACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTgg             >  1:421564/1‑148 (MQ=255)
                                                                                                                                         ggggACCGTTTTACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTGGaa           >  8:119644/1‑149 (MQ=255)
                                                                                                                                         ggggACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGACCTCGCGGCGACGGCGGGGCAGTACCCGCTCGGTATAACAGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTGGaa           <  8:120480/149‑1 (MQ=255)
                                                                                                                                              ccGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCAACCTTCCACTACCAGTTCCACAGCCGCCTGGAACGCCa      >  4:361956/1‑149 (MQ=255)
                                                                                                                                               cGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTGGAACGCCAc     >  5:32288/1‑149 (MQ=255)
                                                                                                                                               cGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACAGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCAACAGCCGACTGGAACGcc       >  7:163093/1‑147 (MQ=255)
                                                                                                                                                 tAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTGGAACGCCACGt   >  8:287199/1‑149 (MQ=255)
                                                                                                                                                  aaaaCGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTGGAACGCCACGtt  >  6:51675/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GTGTCGGGCGACACCGATGTGGAAGAGGCAGTGGATGTGATGCACCTGGGGGTGGTGGACTTCCTGCTCAAGCCGGTGGATCTGGGCAAGCTGTTGGAGCTGGTGAACAAAGAACTGAAAATTGGATGATGCGCCAGGGGGACCGTAAAACGGTCCCCCTGGCAGCAGATGCTGCTTACAACCCGTTACGCGCCTTGAACTCGCGGCGACGGCGGTGCAGTACCGGCTCGGTATAACCGTTCGGTTGCTTGCCACCTTCCACTACCAGTTCCACAGCCGCCTGGAACGCCACGTT  >  NC_002947/432847‑433141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑