Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,329,642 T→G 5.2% L92R (CTG→CGG)  PP_4690 → Rieske 2Fe‑2S family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,329,6420TG 5.2% 351.5 / 6.2 109L92R (CTG→CGG) PP_4690Rieske 2Fe‑2S family protein
Reads supporting (aligned to +/- strand):  ref base T (46/57);  new base G (3/3);  total (49/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

CACCGGGACATCCCGCTGAACTGGGCAGAGGACGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAGGCCAGCACCTCCACCCCGTCCGCTACGGCAGCGCGCAGCGCCTGG  >  NC_002947/5329494‑5329789
                                                                                                                                                      |                                                                                                                                                    
cACCGGGACATCCCGCTGAACTGGGCAGAGGACGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCt                                                                                                                                                      >  3:183908/1‑149 (MQ=255)
     ggACATCCCGCTGAACTGGGCAGAGGACGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCt                                                                                                                                                 <  3:251900/149‑1 (MQ=255)
     ggACATCCCGCTGAACTGGGCAGAGGACGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCAGGGGCt                                                                                                                                                 >  7:362763/1‑149 (MQ=255)
     ggACATCCCGCTGAACTGGGCAGAGGACGCGTTTCTGGATGACAGCACCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCt                                                                                                                                                 <  5:171647/149‑1 (MQ=255)
      gACATCCCGCTGAACTGGGCAGAGGACGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTg                                                                                                                                                <  3:350371/149‑1 (MQ=255)
      gACATCCCGCTGAACTGGGCAGAGGACGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCAGGGGCTg                                                                                                                                                <  8:362763/149‑1 (MQ=255)
         aTCCCGCTGAACTGGGCAGAGGACGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCa                                                                                                                                             <  6:3666/149‑1 (MQ=255)
         aTCCCGCTGAACTGGGCAGAGGACGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCAGGGGCTGcaa                                                                                                                                             <  8:120767/149‑3 (MQ=255)
          tCCCGCTGAACTGGGCAGAGGACGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCAc                                                                                                                                            >  4:406537/1‑149 (MQ=255)
          tCCCGCTGAACTGGGCAGAGGACGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCAc                                                                                                                                            >  8:298016/1‑149 (MQ=255)
          tCCCGCTGAACTGGGCAGAGGACGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCAc                                                                                                                                            >  2:384482/1‑149 (MQ=255)
             cGCTGAACTGGGCAGAGGACGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACg                                                                                                                                           >  1:340206/1‑147 (MQ=255)
             cGCTGAACTGGGCAGAGGACGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGaa                                                                                                                                         >  7:293984/1‑149 (MQ=255)
             cGCTGAACTGGGCAGAGGACGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCAGGGGCTGcattga                                                                                                                                          >  7:344992/1‑143 (MQ=255)
              gCTGAACTGGGCAGAGGACGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACg                                                                                                                                           >  7:308406/1‑146 (MQ=255)
                  aaCTGGGCAGAGGACGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACa                                                                                                                                     <  2:122330/148‑1 (MQ=255)
                  aaCTGGGCAGAGGACGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACa                                                                                                                                     <  4:183908/148‑1 (MQ=255)
                  aaCTGGGCAGAGGACGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAg                                                                                                                                    >  2:254471/1‑149 (MQ=255)
                    cTGGGCAGAGGACGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGcc                                                                                                                                  <  1:58267/149‑1 (MQ=255)
                    cTGGGCAGAGGACGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGcc                                                                                                                                  <  5:21176/149‑1 (MQ=255)
                       ggCAGAGGACGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGGTGCCACGAAGACAGCCAgg                                                                                                                               >  6:261767/1‑149 (MQ=255)
                       ggCAGAGGACGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCa                                                                                                                                 <  2:56322/147‑1 (MQ=255)
                            agGACGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATc                                                                                                                          <  1:29527/149‑1 (MQ=255)
                            agGACGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCACTGGGCTGCCACGAAGACAGCCAGGGCATc                                                                                                                          >  3:109539/1‑149 (MQ=255)
                             ggACGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGCTCTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATc                                                                                                                          <  1:348438/149‑1 (MQ=255)
                              gACGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑TTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCT‑GGCTGCCACGAAGACAGCCAGGGCATCTgg                                                                                                                       >  4:241418/1‑149 (MQ=255)
                              gACGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTg                                                                                                                        <  8:53672/149‑1 (MQ=255)
                               aCGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTgg                                                                                                                       <  5:194895/149‑1 (MQ=255)
                                  cgTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTc                                                                                                                    <  1:162966/149‑1 (MQ=255)
                                   gTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGc                                                                                                                                                  <  4:299018/118‑1 (MQ=255)
                                   gTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGc                                                                                                                                                  >  3:299018/1‑118 (MQ=255)
                                    tttCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTc                                                                                                                    >  3:345273/1‑147 (MQ=255)
                                      tCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAg                                                                                                                <  8:58155/148‑1 (MQ=255)
                                       cTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAgg                                                                                                               <  8:308406/148‑1 (MQ=255)
                                       cTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCAACGAAGACAGCCAGGGCATCTGGTTC‑AAggg                                                                                                              >  1:262853/1‑149 (MQ=255)
                                          gATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAggg                                                                                                              <  1:280731/146‑1 (MQ=255)
                                              aCAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGGGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTcag                                                                                                         >  5:83343/1‑147 (MQ=255)
                                              aCAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTcagca                                                                                                       <  1:311262/149‑1 (MQ=255)
                                              aCAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTcagc                                                                                                        <  3:271454/148‑1 (MQ=255)
                                               cAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTcagcag                                                                                                      >  3:389566/1‑149 (MQ=255)
                                                aGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTcagcag                                                                                                      >  3:286163/1‑148 (MQ=255)
                                                    ccAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAggg                                                                                                                              <  5:364578/121‑1 (MQ=255)
                                                    ccAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCg                                                                                                 <  2:262853/149‑1 (MQ=255)
                                                            aTCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAgcgg                                                                                         >  4:396630/1‑149 (MQ=255)
                                                             tCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAgcggc                                                                                        <  7:151603/149‑1 (MQ=255)
                                                               cACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGcga                                                                                      >  3:124881/1‑149 (MQ=255)
                                                               cACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGcga                                                                                      >  1:50468/1‑149 (MQ=255)
                                                                aCTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGcgat                                                                                     >  7:395997/1‑149 (MQ=255)
                                                                    cgcCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTCAAAGGGGTCAGCAGCACCGGCAAGCGGcgatc                                                                                    <  1:254471/147‑1 (MQ=255)
                                                                    cgcCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGcgatcga                                                                                  <  7:196567/148‑1 (MQ=255)
                                                                     gcCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGcgatcgat                                                                                 >  4:8069/1‑148 (MQ=255)
                                                                     gcCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCT‑GGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGa                                                                               <  3:241418/149‑1 (MQ=255)
                                                                        cATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGAc                                                                              >  1:55193/1‑148 (MQ=255)
                                                                         aTCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAAAGC‑GTGGCAGGCCCCTGTGAGGGTCAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGCGCACAGCAGCACCGGCAAGCGGCGATCTATGACg                                                                             >  5:253036/1‑148 (MQ=255)
                                                                               gTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCt                                                                      <  5:443942/149‑1 (MQ=255)
                                                                                  cgcTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCg                                                                    <  8:293984/148‑1 (MQ=255)
                                                                                   gcTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGGGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGc                                                                  <  6:83343/149‑1 (MQ=255)
                                                                                        tCCTGATAGAAAGCGGCCAATGC‑GTGGCAGGCCCGTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTcc                                                              <  6:252192/148‑1 (MQ=255)
                                                                                         ccTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAg                                                            >  3:291564/1‑149 (MQ=255)
                                                                                           tGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGcgatcg                                                                                   >  5:329439/1‑124 (MQ=255)
                                                                                           tGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGcgatcg                                                                                   <  6:329439/124‑1 (MQ=255)
                                                                                           tGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGcc                                                          >  7:366185/1‑149 (MQ=255)
                                                                                           tGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGcc                                                          >  3:82795/1‑149 (MQ=255)
                                                                                               aGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGt                                                      >  7:225057/1‑149 (MQ=255)
                                                                                                gAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTT‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTg                                                     <  6:212804/149‑1 (MQ=255)
                                                                                                gAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGa                                                        <  7:300098/146‑1 (MQ=255)
                                                                                                  aaGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGcc                                                   <  8:290149/149‑1 (MQ=255)
                                                                                                  aaGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCACTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGcc                                                   <  4:109539/149‑1 (MQ=255)
                                                                                                    gcggcgAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCg                                                                                                 <  8:286703/101‑1 (MQ=255)
                                                                                                      ggcgAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAg                                               <  3:8069/149‑1 (MQ=255)
                                                                                                      ggcgAATGC‑GTGGCAGGCCCCTGGGAGGGTGAGAAGTTGCAG‑CCCCGGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAg                                               <  4:286163/149‑1 (MQ=255)
                                                                                                        cgAATTC‑GTGGCAGGCGCCTGTGATGGTGAGCAGTTGCAG‑GCACTGGGCTGCCATGAAGAATGCCAGGGGATCTGGTTC‑ATGGTGTCAGCAGCACCGGCAAGCGGCGATTGATGACTATACCGTCGGCGTCCAGGCGAGTGCCATAGGc                                             <  4:345767/149‑1 (MQ=255)
                                                                                                        cgAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAGGc                                             <  4:345273/149‑1 (MQ=255)
                                                                                                          aaTGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAGGCCa                                           <  4:291564/149‑1 (MQ=255)
                                                                                                           aTGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAGGc                                             <  2:401470/146‑1 (MQ=255)
                                                                                                           aTGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAGGc                                             >  1:401470/1‑146 (MQ=255)
                                                                                                             gC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAGGCCAGCa                                        >  8:14599/1‑149 (MQ=255)
                                                                                                                 tGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAGGCCAGCACCt                                     >  7:261229/1‑149 (MQ=255)
                                                                                                                  ggCAGGCCCCTGTGAGGGTGAGCAGTTGCAGTCCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAGGCCAGCACCt                                     >  1:46032/1‑149 (MQ=255)
                                                                                                                   gCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAGGCCAGCACCTcc                                   <  3:160247/149‑1 (MQ=255)
                                                                                                                     aGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGCTACCCTCGGCGTCCAGCCGAGTGCCATAGGGCAGCACCTacac                                 >  6:304248/1‑149 (MQ=255)
                                                                                                                     aGGCCCCTGTGAGGGTGAGCAATTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAGGCCAGCACCTCCAc                                 <  6:312849/149‑1 (MQ=255)
                                                                                                                      ggCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAGGCCAGCACCTCCAcc                                <  4:389566/149‑1 (MQ=255)
                                                                                                                          ccTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAGGCCAGCACCTCCACCCCGt                            >  7:120113/1‑149 (MQ=255)
                                                                                                                               gAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAGGCCAGCACCTCCACCCCGTCCGc                        >  6:250030/1‑148 (MQ=255)
                                                                                                                                    tGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAGGCCAGCACCTCCACCCCGTCCGCTACgg                   >  4:376418/1‑148 (MQ=255)
                                                                                                                                     gAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAGGCCAGCACCTCCACCCCGTCCGCTACgg                   >  7:368857/1‑147 (MQ=255)
                                                                                                                                      aGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAGGCCAGCACCTCCACCCCGTCCGCTACGgca                 <  3:396630/148‑1 (MQ=255)
                                                                                                                                        tcgtggCAG‑CCCAGGGGCTGCAACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAGGCCAGCACCTCCACCCCGTCCGCTACGgcagcg              >  6:80553/6‑149 (MQ=255)
                                                                                                                                        cAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AGGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAGGCCAGCACCTCCACCCCGTCCGCTACGgcagcg              >  7:136570/1‑149 (MQ=255)
                                                                                                                                          gTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAGGCCAGCACCTCCACCCCGTCCGCTACGgcagcgcg            <  8:225057/149‑1 (MQ=255)
                                                                                                                                             gCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAGGCCAGCACCTCCACCCCGTCCGCTACGgcagcgcgcag         >  6:368940/1‑149 (MQ=255)
                                                                                                                                             gCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAGGCCAGCACCTCCACCCCGTCCGCTACGgcagcgcgca          <  4:290874/148‑1 (MQ=255)
                                                                                                                                             gCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAGGCCAGCACCTCCACCCCGTCCGCTACGgcagcgcgca          <  3:406537/148‑1 (MQ=255)
                                                                                                                                              cAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCa                                                  >  2:430984/1‑107 (MQ=255)
                                                                                                                                               aG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCCAGAGGCGAGCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAGGCCAGCACCTCCACCCCGTCCGCTACGgcagcgcgcagcg       >  3:11516/1‑149 (MQ=255)
                                                                                                                                                  ccccTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAGGCCAGCACCTCCACCCCGTCCGCTACGGCAGCGCGCAGCGcc     <  4:82795/149‑1 (MQ=255)
                                                                                                                                                   cccTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAGGCCAGCACCTCCACCCCGTCCGCTACGGCAGCGCGCAGCGCCt    <  8:366185/149‑1 (MQ=255)
                                                                                                                                                   cccTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAGGCCAGCACCTCCACCCCGTCCGCTACGGCAGCGCGCAGCGCCt    >  1:181952/1‑149 (MQ=255)
                                                                                                                                                     cTGGGCTGCCACGAATACAGCCAGTGCATCTGGTTC‑AAGGGGTCAGGAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGGCGAGTGCCATAGGGCAGCACCTCCACCCCGTGCGCTACGGCAGCGCGCAGCGCCTgg  <  6:253036/149‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
CACCGGGACATCCCGCTGAACTGGGCAGAGGACGCGTTTCTGGATGACAGCGCCAGCCTCATCCACTGCGCCCATCACGGTGCGCTGTTCCTGATAGAAAGCGGCGAATGC‑GTGGCAGGCCCCTGTGAGGGTGAGCAGTTGCAG‑CCCCTGGGCTGCCACGAAGACAGCCAGGGCATCTGGTTC‑AAGGGGTCAGCAGCACCGGCAAGCGGCGATCGATGACGATACCCTCGGCGTCCAGCCGAGTGCCATAGGCCAGCACCTCCACCCCGTCCGCTACGGCAGCGCGCAGCGCCTGG  >  NC_002947/5329494‑5329789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑