Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,333,664 C→A 8.0% L32M (CTG→ATG) ‡ PP_4695 → two‑component system sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,333,6640CA 8.0% 236.2 / 7.8 75L32M (CTG→ATG) ‡PP_4695two‑component system sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base C (35/34);  new base A (2/4);  total (37/38)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

TACCTGCTCTGGCTGATCCTGATCGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCG  >  NC_002947/5333516‑5333811
                                                                                                                                                    |                                                                                                                                                   
tACCTGCTCTGGCTGATCCTGATCGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGAGCTGGc                                                                                                                                                     >  4:29295/1‑149 (MQ=255)
      ctctGGCTGATCCTGATCGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATcc                                                                                                                                               >  4:370836/1‑149 (MQ=255)
        ctGGCTGATCCTGATCGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGCATCAGCGAGCGCGGGCTGATCCcg                                                                                                                                             >  3:252801/1‑149 (MQ=255)
         tGGCTGATCCTGATCGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGAGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCcgc                                                                                                                                            >  6:328764/1‑149 (MQ=255)
           gCTGATCCTGATCGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCcgc                                                                                                                                            <  6:238239/147‑1 (MQ=255)
           gCTGATCCTGATCGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGt                                                                                                                                          >  6:276658/1‑149 (MQ=255)
           gCTGATCCTGATCGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGt                                                                                                                                          <  7:142095/149‑1 (MQ=255)
           gCTGATCCTGATCGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCATGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGt                                                                                                                                          >  6:227644/1‑149 (MQ=255)
            ctgatcctgatcGTCGTCACCTTCCTCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGtt                                                                                                                                         <  7:208737/149‑1 (MQ=255)
               atcctgatcGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGa                                                                                                                                      <  5:337797/149‑1 (MQ=255)
               atcctgatcGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGa                                                                                                                                      <  3:240832/149‑1 (MQ=255)
               atcctgatcGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAACGCGGGATCAGCCCGCGCTCg                                                                                                                                       <  2:322908/148‑1 (MQ=255)
                tcctgatcGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAACGCGGGATCAGCCCGCGCTCgc                                                                                                                                      <  6:287962/148‑2 (MQ=255)
                tcctgatcGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCCCGCGCTCGCTGATccag                                                                                                                                             <  5:96638/141‑3 (MQ=255)
                tcctgatcGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCCCGCGCTCGCTGATccag                                                                                                                                             >  6:96638/1‑139 (MQ=255)
                  ctgatcGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATGAGCGAGCGCGGGCTGATCCCGCGTTCGATc                                                                                                                                    <  8:107213/148‑1 (MQ=255)
                  ctgatcGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAACGCGGGATCAGCCCGCGCTCgctga                                                                                                                                   <  1:394436/149‑5 (MQ=255)
                   tgatcGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCAt                                                                                                                                  >  6:35936/1‑149 (MQ=255)
                    gatcGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCAtt                                                                                                                                 >  7:236226/1‑149 (MQ=255)
                     atcGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTc                                                                                                                                >  1:425826/1‑149 (MQ=255)
                     atcGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAACGCGGGATCAGCCCGCGCTCgc                                                                                                                                      >  4:287468/1‑142 (MQ=255)
                     atcGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAACGCGGGATCAGCCCGCGCTCgc                                                                                                                                      <  3:287468/143‑2 (MQ=255)
                         tcgtcACCTTCATCCTGCAGAGCCAACGCTATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGcca                                                                                                                            <  5:252667/149‑1 (MQ=255)
                            tcACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGccacc                                                                                                                          >  6:241673/1‑148 (MQ=255)
                              aCCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCATGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGccacccac                                                                                                                       <  5:227644/149‑1 (MQ=255)
                               ccTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGc                                                                                                                                                     >  4:294494/1‑118 (MQ=255)
                               ccTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGc                                                                                                                                                     <  3:294494/118‑1 (MQ=255)
                                cTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCTTGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACt                                                                                                                      >  1:140344/1‑148 (MQ=255)
                                cTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACt                                                                                                                      >  5:199342/1‑148 (MQ=255)
                                   cATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTAc                                                                                                                   <  8:236226/148‑1 (MQ=255)
                                     tCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTa                                                                                                                >  7:36295/1‑149 (MQ=255)
                                      ccTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTa                                                                                                                >  2:338530/1‑148 (MQ=255)
                                       cTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTac                                                                                                               <  5:328764/148‑1 (MQ=255)
                                         gCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACAcc                                                                                                            >  5:98075/1‑149 (MQ=255)
                                            gagCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTg                                                                                                         <  4:252801/149‑1 (MQ=255)
                                                cAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGc                                                                                                     <  7:257254/149‑1 (MQ=255)
                                                  aCGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTg                                                                                                   >  7:83840/1‑149 (MQ=255)
                                                   cGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTgg                                                                                                  <  1:389650/149‑1 (MQ=255)
                                                    gagaTGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTggg                                                                                                 >  1:122858/1‑149 (MQ=255)
                                                           cGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTAc                                                                                          >  5:81690/1‑149 (MQ=255)
                                                           cGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTAc                                                                                          <  8:110831/149‑1 (MQ=255)
                                                                aGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCTTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCa                                                                                      <  6:98075/148‑1 (MQ=255)
                                                                  cTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACt                                                                                                                      <  1:98564/114‑1 (MQ=255)
                                                                  cTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACt                                                                                                                      >  2:98564/1‑114 (MQ=255)
                                                                  cTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTg                                                                                   >  7:325690/1‑149 (MQ=255)
                                                                   tttAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGc                                                                                  >  4:395581/1‑149 (MQ=255)
                                                                       gCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTgg                                                                              <  5:276658/149‑1 (MQ=255)
                                                                        ccTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTggg                                                                             <  8:36295/149‑1 (MQ=255)
                                                                              ccAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTAc                                                                                                                   >  2:436419/1‑105 (MQ=255)
                                                                              ccAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTc                                                                        >  1:237168/1‑148 (MQ=255)
                                                                                 gaagatCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCATGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATg                                                                    <  8:166832/146‑1 (MQ=255)
                                                                                         tGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGtt                                                            >  2:89150/1‑149 (MQ=255)
                                                                                           aTCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTtgg                                                          >  3:239643/1‑149 (MQ=255)
                                                                                           aTCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTtg                                                           <  6:199342/148‑1 (MQ=255)
                                                                                            tCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTtggc                                                         <  2:237168/149‑1 (MQ=255)
                                                                                                     ggTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTAc                                                <  1:338530/149‑1 (MQ=255)
                                                                                                      gTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTAc                                                >  3:359991/1‑148 (MQ=255)
                                                                                                       tACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTtggcctggc                                                    >  6:383870/1‑143 (MQ=255)
                                                                                                       tACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTtggcctggc                                                    <  5:383870/143‑1 (MQ=255)
                                                                                                       tACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTtg                                                           >  3:403072/1‑136 (MQ=255)
                                                                                                       tACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTtg                                                           <  4:403072/136‑1 (MQ=255)
                                                                                                          cTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGta                                           >  6:339609/1‑149 (MQ=255)
                                                                                                             aTGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGtacgg                                        >  2:255476/1‑149 (MQ=255)
                                                                                                             aTGGTGCTGTGCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGtacg                                         <  5:189659/148‑1 (MQ=255)
                                                                                                                gTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGtacggcta                                     <  2:122858/149‑1 (MQ=255)
                                                                                                                gTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGtacggct                                      <  5:339609/148‑1 (MQ=255)
                                                                                                                        cGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTc                              <  2:425826/148‑1 (MQ=255)
                                                                                                                         ggCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTcc                             <  5:35936/148‑1 (MQ=255)
                                                                                                                            gTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGc                         <  1:89150/149‑1 (MQ=255)
                                                                                                                             tGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGcc                        <  3:370836/149‑1 (MQ=255)
                                                                                                                                      tgatcgaacgCGGGATCAGCCCGCGCTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTg               >  5:378060/5‑149 (MQ=255)
                                                                                                                                       cAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTgg              >  2:96141/1‑149 (MQ=255)
                                                                                                                                         gcgagcgCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTgggg            >  6:292591/1‑149 (MQ=255)
                                                                                                                                          cgagcgCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTggggg           >  3:189438/1‑149 (MQ=255)
                                                                                                                                                cgGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTg     >  2:374443/1‑149 (MQ=255)
                                                                                                                                                  ggCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGgc   <  2:140344/149‑1 (MQ=255)
                                                                                                                                                  ggCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGgc   <  1:255476/149‑1 (MQ=255)
                                                                                                                                                   gCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTAGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGgcg  >  3:118805/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TACCTGCTCTGGCTGATCCTGATCGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCG  >  NC_002947/5333516‑5333811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑