Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,333,668 T→G 7.9% I33S (ATC→AGC)  PP_4695 → two‑component system sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,333,6680TG 7.9% 228.4 / 10.3 76I33S (ATC→AGC) PP_4695two‑component system sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base T (36/34);  new base G (2/4);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.23e-01

CTCTGGCTGATCCTGATCGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATT  >  NC_002947/5333522‑5333815
                                                                                                                                                  |                                                                                                                                                   
ctctGGCTGATCCTGATCGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATcc                                                                                                                                                   >  4:370836/1‑149 (MQ=255)
  ctGGCTGATCCTGATCGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGCATCAGCGAGCGCGGGCTGATCCcg                                                                                                                                                 >  3:252801/1‑149 (MQ=255)
   tGGCTGATCCTGATCGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGAGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCcgc                                                                                                                                                >  6:328764/1‑149 (MQ=255)
     gCTGATCCTGATCGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCcgc                                                                                                                                                <  6:238239/147‑1 (MQ=255)
     gCTGATCCTGATCGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGt                                                                                                                                              <  7:142095/149‑1 (MQ=255)
     gCTGATCCTGATCGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGt                                                                                                                                              >  6:276658/1‑149 (MQ=255)
     gCTGATCCTGATCGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCATGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGt                                                                                                                                              >  6:227644/1‑149 (MQ=255)
      ctgatcctgatcGTCGTCACCTTCCTCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGtt                                                                                                                                             <  7:208737/149‑1 (MQ=255)
         atcctgatcGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGa                                                                                                                                          <  3:240832/149‑1 (MQ=255)
         atcctgatcGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGa                                                                                                                                          <  5:337797/149‑1 (MQ=255)
         atcctgatcGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAACGCGGGATCAGCCCGCGCTCg                                                                                                                                           <  2:322908/148‑1 (MQ=255)
          tcctgatcGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAACGCGGGATCAGCCCGCGCTCgc                                                                                                                                          <  6:287962/148‑2 (MQ=255)
          tcctgatcGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCCCGCGCTCGCTGATccag                                                                                                                                                 >  6:96638/1‑139 (MQ=255)
          tcctgatcGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCCCGCGCTCGCTGATccag                                                                                                                                                 <  5:96638/141‑3 (MQ=255)
            ctgatcGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATGAGCGAGCGCGGGCTGATCCCGCGTTCGATc                                                                                                                                        <  8:107213/148‑1 (MQ=255)
            ctgatcGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAACGCGGGATCAGCCCGCGCTCgctga                                                                                                                                       <  1:394436/149‑5 (MQ=255)
             tgatcGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCAt                                                                                                                                      >  6:35936/1‑149 (MQ=255)
              gatcGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCAtt                                                                                                                                     >  7:236226/1‑149 (MQ=255)
               atcGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTc                                                                                                                                    >  1:425826/1‑149 (MQ=255)
               atcGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAACGCGGGATCAGCCCGCGCTCgc                                                                                                                                          >  4:287468/1‑142 (MQ=255)
               atcGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAACGCGGGATCAGCCCGCGCTCgc                                                                                                                                          <  3:287468/143‑2 (MQ=255)
                   tcgtcACCTTCATCCTGCAGAGCCAACGCTATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGcca                                                                                                                                <  5:252667/149‑1 (MQ=255)
                      tcACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGccacc                                                                                                                              >  6:241673/1‑148 (MQ=255)
                        aCCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCATGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGccacccac                                                                                                                           <  5:227644/149‑1 (MQ=255)
                          cTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCTTGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACt                                                                                                                          >  1:140344/1‑148 (MQ=255)
                          cTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACt                                                                                                                          >  5:199342/1‑148 (MQ=255)
                             cATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTAc                                                                                                                       <  8:236226/148‑1 (MQ=255)
                               tCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTa                                                                                                                    >  7:36295/1‑149 (MQ=255)
                                ccTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTa                                                                                                                    >  2:338530/1‑148 (MQ=255)
                                 cTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTac                                                                                                                   <  5:328764/148‑1 (MQ=255)
                                   gCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACAcc                                                                                                                >  5:98075/1‑149 (MQ=255)
                                      gagCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTg                                                                                                             <  4:252801/149‑1 (MQ=255)
                                          cAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGc                                                                                                         <  7:257254/149‑1 (MQ=255)
                                            aCGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTg                                                                                                       >  7:83840/1‑149 (MQ=255)
                                             cGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTgg                                                                                                      <  1:389650/149‑1 (MQ=255)
                                              gagaTGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTggg                                                                                                     >  1:122858/1‑149 (MQ=255)
                                                     cGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTAc                                                                                              >  5:81690/1‑149 (MQ=255)
                                                     cGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTAc                                                                                              <  8:110831/149‑1 (MQ=255)
                                                          aGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCTTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCa                                                                                          <  6:98075/148‑1 (MQ=255)
                                                            cTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACt                                                                                                                          >  2:98564/1‑114 (MQ=255)
                                                            cTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACt                                                                                                                          <  1:98564/114‑1 (MQ=255)
                                                            cTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTg                                                                                       >  7:325690/1‑149 (MQ=255)
                                                             tttAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGc                                                                                      >  4:395581/1‑149 (MQ=255)
                                                                 gCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTgg                                                                                  <  5:276658/149‑1 (MQ=255)
                                                                  ccTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTggg                                                                                 <  8:36295/149‑1 (MQ=255)
                                                                        ccAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTAc                                                                                                                       >  2:436419/1‑105 (MQ=255)
                                                                        ccAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTc                                                                            >  1:237168/1‑148 (MQ=255)
                                                                           gaagatCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCATGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATg                                                                        <  8:166832/146‑1 (MQ=255)
                                                                                   tGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGtt                                                                >  2:89150/1‑149 (MQ=255)
                                                                                     aTCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTtgg                                                              >  3:239643/1‑149 (MQ=255)
                                                                                     aTCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTtg                                                               <  6:199342/148‑1 (MQ=255)
                                                                                      tCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTtggc                                                             <  2:237168/149‑1 (MQ=255)
                                                                                               ggTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTAc                                                    <  1:338530/149‑1 (MQ=255)
                                                                                                gTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTAc                                                    >  3:359991/1‑148 (MQ=255)
                                                                                                 tACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTtggcctggc                                                        <  5:383870/143‑1 (MQ=255)
                                                                                                 tACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTtggcctggc                                                        >  6:383870/1‑143 (MQ=255)
                                                                                                 tACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTtg                                                               >  3:403072/1‑136 (MQ=255)
                                                                                                 tACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTtg                                                               <  4:403072/136‑1 (MQ=255)
                                                                                                    cTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGta                                               >  6:339609/1‑149 (MQ=255)
                                                                                                       aTGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGtacgg                                            >  2:255476/1‑149 (MQ=255)
                                                                                                       aTGGTGCTGTGCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGtacg                                             <  5:189659/148‑1 (MQ=255)
                                                                                                          gTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGtacggcta                                         <  2:122858/149‑1 (MQ=255)
                                                                                                          gTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGtacggct                                          <  5:339609/148‑1 (MQ=255)
                                                                                                                  cGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTc                                  <  2:425826/148‑1 (MQ=255)
                                                                                                                   ggCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTcc                                 <  5:35936/148‑1 (MQ=255)
                                                                                                                      gTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGc                             <  1:89150/149‑1 (MQ=255)
                                                                                                                       tGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGcc                            <  3:370836/149‑1 (MQ=255)
                                                                                                                                tgatcgaacgCGGGATCAGCCCGCGCTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTg                   >  5:378060/5‑149 (MQ=255)
                                                                                                                                 cAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTgg                  >  2:96141/1‑149 (MQ=255)
                                                                                                                                   gcgagcgCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTgggg                >  6:292591/1‑149 (MQ=255)
                                                                                                                                    cgagcgCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTggggg               >  3:189438/1‑149 (MQ=255)
                                                                                                                                          cgGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTg         >  2:374443/1‑149 (MQ=255)
                                                                                                                                            ggCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGgc       <  1:255476/149‑1 (MQ=255)
                                                                                                                                            ggCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGgc       <  2:140344/149‑1 (MQ=255)
                                                                                                                                             gCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTAGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGgcg      >  3:118805/1‑149 (MQ=255)
                                                                                                                                                 aTCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGTCCTACCAGTACGGCTACGGCTTCCTGTCATGTTACCTGGGGGTGGCTGGGGCagt  >  8:139297/1‑147 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CTCTGGCTGATCCTGATCGTCGTCACCTTCATCCTGCAGAGCCAACGAGATGCCGATGAGCTTTAGCCTGACCCAGATGATCCTGATCAGCGCCGGGTACCTCATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATT  >  NC_002947/5333522‑5333815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑