BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,339,896	G→T	7.8%	R375L (CGC→CTC) ‡	pcnB →	poly(A) polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,339,896	0	G	T	7.8%	310.8 / 9.0	99	R375L (CGC→CTC) ‡	pcnB	poly(A) polymerase
Reads supporting (aligned to +/- strand): ref base G (41/50); new base T (4/4); total (45/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 104 total aligned reads displayed.

CACCTGCAGAACCAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGACA  >  NC_002947/5339748‑5340039

cacaTGCAGAACCAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGCCCTGATCGCCGCGCAGTGCGCGCGCATTGCCATCCCCAAGCGTATTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGCAGCGCCTgccgcg                                                                                                                                                  >  8:113184/1‑149 (MQ=255)

cACCTGCAGAACCAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCTGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTgccgcg                                                                                                                                                  >  2:161728/1‑149 (MQ=255)

   cTGCAGAACCAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTgccgcgccg                                                                                                                                               >  1:340864/1‑149 (MQ=255)

       aGAACCAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGc                                                                                                                                           <  6:409896/149‑1 (MQ=255)

        gAACCAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCg                                                                                                                                          <  4:397350/149‑1 (MQ=255)

        gAACCAGGCCGGGCCGCCGATCCCGGCCATGAACGGCGCGGCCCACGTCCTGATCGCCGAGCAGGGCGCGCGCATTGCCATCCCCCCGGGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCg                                                                                                                                          <  5:232181/149‑1 (MQ=255)

         aaCCAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCgg                                                                                                                                         <  8:77348/149‑1 (MQ=255)

         aaCCAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCgg                                                                                                                                         >  1:356839/1‑149 (MQ=255)

            cAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCTGCGGCGCGGCag                                                                                                                                      <  3:281934/149‑2 (MQ=255)

            cAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCaa                                                                                                                                      <  3:215438/149‑1 (MQ=255)

             aGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAg                                                                                                                                     <  2:356839/149‑1 (MQ=255)

                gCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTAATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCTTGCCGCTGCGGCGCGGCAGgcgc                                                                                                                                  <  6:263888/149‑1 (MQ=255)

                 cGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAgcgc‑g                                                                                                                                >  1:431645/1‑149 (MQ=255)

                    gccgccGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑Gcc                                                                                                                              >  7:84150/1‑148 (MQ=255)

                    gccgccGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑Gcc                                                                                                                              >  2:359911/1‑148 (MQ=255)

                     ccgccgATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGa                                                                                                                            >  6:14549/1‑149 (MQ=255)

                     ccgccgATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTCCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCTTGCCGCTGCGGCGCGGCAGGCGC‑Tcct                                                                                                                             <  4:152096/148‑2 (MQ=255)

                              ccGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATgctgct                                                                                                                   <  3:160267/149‑1 (MQ=255)

                              ccGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATgctgct                                                                                                                   <  7:190804/149‑1 (MQ=255)

                               cGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGTACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGctgctg                                                                                                                  >  2:143268/1‑149 (MQ=255)

                               cGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGAAACGGAAGGCGCGCCTGCCGCTCAGCAGCGGCAAGCGC‑GCCGACAtgctccgg                                                                                                                  >  4:364030/1‑145 (MQ=255)

                               cGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCACCAAGCGATTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGAGCCTGCCGCTCCGCAGCTGCCAGCGC‑CCCGACATgctcctc                                                                                                                  >  6:253706/1‑148 (MQ=255)

                                ggCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTgg                                                                                                                 <  7:348333/149‑1 (MQ=255)

                                   cATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGAc                                                                                                               >  6:135301/1‑148 (MQ=255)

                                    aTGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACaa                                                                                                             >  4:306865/1‑149 (MQ=255)

                                     tGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAAc                                                                                                            <  4:120866/149‑1 (MQ=255)

                                         cGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCcgc                                                                                                        <  6:305385/149‑1 (MQ=255)

                                           gTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGTAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGt                                                                                                      <  5:131670/149‑1 (MQ=255)

                                                   cACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCgcgc                                                                                              >  5:333694/1‑149 (MQ=255)

                                                    aCGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGcc                                                                                             <  7:406048/149‑1 (MQ=255)

                                                         cTGATCGCTGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGATA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTa                                                                                        <  1:161728/149‑1 (MQ=255)

                                                              cGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACt                                                                                   >  6:254579/1‑149 (MQ=255)

                                                               gCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACt                                                                                   <  6:234004/148‑1 (MQ=255)

                                                                ccGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCcg                                                                                                 <  7:214705/133‑1 (MQ=255)

                                                                ccGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCcg                                                                                                 >  8:214705/1‑133 (MQ=255)

                                                                   aGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCctg                                                                              >  3:150604/1‑149 (MQ=255)

                                                                     cAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCctgct                                                                            >  5:302985/1‑149 (MQ=255)

                                                                     cAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCctgct                                                                            >  8:175228/1‑149 (MQ=255)

                                                                      aGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCctgctg                                                                           >  5:276867/1‑149 (MQ=255)

                                                                        tGCGCGCGCATTGCCATCCCCAAGCGGTTTACCCTGCCGATCCGCGAGATCTCTGCTACATGTACCAATAGCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCctgctg                                                                           <  1:359911/149‑1 (MQ=255)

                                                                          cgcgcgcATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGATA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCctgctgctg                                                                        <  1:143268/148‑1 (MQ=255)

                                                                           gcgcacATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCcgc                                                                                                <  4:247717/123‑1 (MQ=255)

                                                                           gcgcacATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCcgc                                                                                                >  3:247717/1‑123 (MQ=255)

                                                                               gcATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGaa                                                                  <  5:135301/149‑1 (MQ=255)

                                                                                cATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGaaa                                                                 <  2:340864/149‑1 (MQ=255)

                                                                                cATTGCCATCCCCAAGCGGTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGaaa                                                                 <  4:28672/149‑1 (MQ=255)

                                                                                  ttGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑CCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAgc                                                               >  5:237193/1‑149 (MQ=255)

                                                                                      cATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCg                                                           >  3:167931/1‑149 (MQ=255)

                                                                                       aTCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTTGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCgg                                                          <  6:433779/149‑1 (MQ=255)

                                                                                          cccAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCgggg                                                        <  7:69988/148‑1 (MQ=255)

                                                                                           ccAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTAc                                                                                       >  8:337721/1‑116 (MQ=255)

                                                                                           ccAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTAc                                                                                       <  7:337721/116‑1 (MQ=255)

                                                                                            cAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCtgctgctgc                                                                       >  6:315446/1‑131 (MQ=255)

                                                                                            cAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCtgctgctgc                                                                       <  5:315446/131‑1 (MQ=255)

                                                                                            cAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCgggg                                                        >  8:371586/1‑146 (MQ=255)

                                                                                             actcGTATTACCCTGCCGATCAGCGAGA‑‑GCTGTGACATGCAGTAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGgaaga                                                    <  8:270175/146‑1 (MQ=255)

                                                                                             aaGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGa                                                                                                                <  7:171856/89‑1 (MQ=255)

                                                                                             aaGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGa                                                                                                                >  8:171856/1‑89 (MQ=255)

                                                                                             aaGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGgaaga                                                    <  2:329362/149‑1 (MQ=255)

                                                                                               gCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGaaa                                                  >  4:361196/1‑149 (MQ=255)

                                                                                               gCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGaaa                                                  >  3:82639/1‑149 (MQ=255)

                                                                                                 gTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAAcc                                                <  3:361196/149‑1 (MQ=255)

                                                                                                          tGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTg                                       >  6:50473/1‑149 (MQ=255)

                                                                                                           gCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGctgctg                                                                                                                  <  4:380771/73‑1 (MQ=255)

                                                                                                           gCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGctgctg                                                                                                                  >  3:380771/1‑73 (MQ=255)

                                                                                                            ccGATCCGCGAGA‑‑TCTGGGACATGCAGGAGAGCCTGCCGCGCCGAAGCGGCAAGAGC‑TCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTggg                                     >  3:144486/1‑149 (MQ=255)

                                                                                                            ccGATCCGCGAGA‑‑TCTAGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGTCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTgg                                      <  7:229502/148‑1 (MQ=255)

                                                                                                             cGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGc                                    >  7:125975/1‑149 (MQ=255)

                                                                                                              caTCTGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGc                                    <  5:418735/147‑1 (MQ=255)

                                                                                                               aTCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCa                                  <  5:254579/149‑1 (MQ=255)

                                                                                                                  cgcgAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtg                               <  4:82639/149‑1 (MQ=255)

                                                                                                                   gcgAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtgg                              >  1:74396/1‑149 (MQ=255)

                                                                                                                   gcgAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtgg                              >  7:98301/1‑149 (MQ=255)

                                                                                                                   gcgAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtgg                              >  2:346012/1‑149 (MQ=255)

                                                                                                                   gcgAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtgg                              <  6:276867/149‑1 (MQ=255)

                                                                                                                           tCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGAcc                        >  2:134040/1‑149 (MQ=255)

                                                                                                                           tCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGAcc                        >  7:267680/1‑149 (MQ=255)

                                                                                                                               ggACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGa                      <  4:413619/147‑1 (MQ=255)

                                                                                                                               ggACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGAt                     <  6:333694/148‑1 (MQ=255)

                                                                                                                               ggACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGAt                     >  3:333403/1‑148 (MQ=255)

                                                                                                                                gACATGCAGGAGCGCCTGCCGGGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGAtt                    <  6:302985/148‑1 (MQ=255)

                                                                                                                                 aCATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑CCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTAc                  <  6:237193/149‑1 (MQ=255)

                                                                                                                                   aTGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCa                <  7:175228/149‑1 (MQ=255)

                                                                                                                                   aTGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAACAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCa                <  3:113469/149‑1 (MQ=255)

                                                                                                                                     gCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGCACTGGGCCAGTGGTGGACCGATTACCAg               >  2:310811/1‑148 (MQ=255)

                                                                                                                                     gCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtggtg                            >  4:247570/1‑135 (MQ=255)

                                                                                                                                     gCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAgtggtg                            <  3:247570/135‑1 (MQ=255)

                                                                                                                                     gCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAg               >  6:282268/1‑148 (MQ=255)

                                                                                                                                     gCAGGAGCGCCTGCCGCGCCGCAGAGGCAAGCGC‑GCAGACATGATGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCAGGGGAAGAACCCGACGAACTGGGCCAGTGGTGGACCGATTACCAgg              >  7:253775/1‑149 (MQ=255)

                                                                                                                                      cAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGa             <  7:371586/149‑1 (MQ=255)

                                                                                                                                       aagAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGAc            <  3:427246/147‑1 (MQ=255)

                                                                                                                                       aGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGAc            <  1:134040/149‑1 (MQ=255)

                                                                                                                                        ggcgcgcTTGCCGCTGCGGCGCGGCAGGCGC‑TCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACg           <  1:145729/146‑1 (MQ=255)

                                                                                                                                         gAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGc          <  3:306865/149‑1 (MQ=255)

                                                                                                                                           gcgcTTGCCGCTGCGGCGCGGCAGGCGCTGCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAgg              >  1:247947/1‑144 (MQ=255)

                                                                                                                                            cgcTTGCCGCTGCGGCGCGGCAGGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCaaa       >  1:161311/1‑149 (MQ=255)

                                                                                                                                              ccTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAAc      >  5:338658/1‑148 (MQ=255)

                                                                                                                                               cTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGa    >  7:123292/1‑149 (MQ=255)

                                                                                                                                                  ccgcgcctcAGCGTCAAGCGC‑TCCGACATGCTGCTGGACAAGCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGTGAAGAAACCGACTAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGACa  <  8:253775/148‑1 (MQ=255)

                                                                                                                                                  ccgcgccgcAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCTGTGGTGGACCGATTACCAGGACGCAAACGACa  <  3:390401/148‑1 (MQ=255)

CACCTGCAGAACCAGGGCGTGCCGCCGATCCCGGCCATGAACGGTGCGGCCCACGACCTGATCGCCGAGCAGTGCGCGCGCATTGCCATCCCCAAGCGTTTTACCCTGCCGATCCGCGAGA‑‑TCTGGGACATGCAGGAGCGCCTGCCGCGCCGCAGCGGCAAGCGC‑GCCGACATGCTGCTGGACAACCCGCGTTTCCGCGCCGGTTACGACTTCCTGCTGCTGCGTGAAAGCGCCGGGGAAGAAACCGACGAACTGGGCCAGTGGTGGACCGATTACCAGGACGCAAACGACA  >  NC_002947/5339748‑5340039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`