BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,350,085	C→G	5.6%	A853A (GCC→GCG) ‡	PP_4704 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,350,085	0	C	G	5.6%	348.3 / 6.9	107	A853A (GCC→GCG) ‡	PP_4704	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (47/54); new base G (3/3); total (50/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01

Only 100 of 109 total aligned reads displayed.

AGCGGCGAGGCCCAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGAATGGGAGCAGT  >  NC_002947/5349939‑5350233

aGCGGCGAGGCCCAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCAc                                                                                                                                                     >  7:177357/1‑149 (MQ=255)

  cggcgAGGCCCAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCt                                                                                                                                                   <  6:218021/149‑1 (MQ=255)

  ccgcgAGGCCCAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGCAACTGCGCCAGGAAATCGAAGGCACCAGAGCCCAGGCCAGCGACGGCCCGATCATAGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCt                                                                                                                                                   >  8:190030/3‑149 (MQ=255)

   ggcgAGGCCCAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTg                                                                                                                                                  <  2:347494/149‑1 (MQ=255)

    gcgAGGCCCAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTgg                                                                                                                                                 >  4:419402/1‑149 (MQ=255)

      gAGGCCCAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTa                                                                                                                                               <  7:24391/149‑1 (MQ=255)

      gAGGCCCAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTa                                                                                                                                               <  7:233885/149‑1 (MQ=255)

         gCCCAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACaa                                                                                                                                            >  3:43143/1‑149 (MQ=255)

           ccAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGa                                                                                                                                          <  6:278994/149‑1 (MQ=255)

           ccAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGa                                                                                                                                          <  8:211858/149‑1 (MQ=255)

              gtcgtcCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACa                                                                                                                                             <  2:79675/143‑1 (MQ=255)

              gtcgtcCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACa                                                                                                                                             >  1:79675/1‑143 (MQ=255)

                 gtcCTTGGACAAGCTGACATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGTGTACCAGGCCACCTGGTACAAGATCCTg                                                                                                                                     <  1:246745/148‑1 (MQ=255)

                  tcCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCa                                                                                                                                   <  6:78021/149‑1 (MQ=255)

                       ggACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCg                                                                                                                                <  3:152433/147‑1 (MQ=255)

                         aCAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACgg                                                                                                                            >  2:81486/1‑149 (MQ=255)

                          cAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGt                                                                                                                                                >  3:16127/1‑128 (MQ=255)

                          cAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGt                                                                                                                                                <  4:16127/128‑1 (MQ=255)

                          cAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACggg                                                                                                                           <  5:404293/149‑1 (MQ=255)

                           aaGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACgggg                                                                                                                          >  3:38140/1‑149 (MQ=255)

                           aaGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACgggg                                                                                                                          >  7:378601/1‑149 (MQ=255)

                            aGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGt                                                                                                                         <  6:114897/149‑1 (MQ=255)

                               tGGCATCCGCGCTGAAAGAGCGCACGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGACCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGa                                                                                                                       >  8:11107/1‑148 (MQ=255)

                                  cATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGTGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAg                                                                                                                   <  1:333082/149‑1 (MQ=255)

                                   aTCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAgg                                                                                                                  >  7:71057/1‑149 (MQ=255)

                                   aTCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAgg                                                                                                                  >  4:193342/1‑149 (MQ=255)

                                    tCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGTGACGGCCCGCTGATTGCCCAACTGCGCCTTGAGCGTGAGTACCAGGTGGCCTGGTActc                                                                                                                                            >  1:112099/1‑120 (MQ=255)

                                    tCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGTGACGGCCCGCTGATTGCCCAACTGCGCCTTGAGCGTGAGTACCAGGTGGCCTGGTActc                                                                                                                                            >  1:106306/1‑120 (MQ=255)

                                    tCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGTGACGGCCCGCTGATTGCCCAACTGCGCCTTGAGCGTGAGTACCAGGTGGCCTGGTActc                                                                                                                                            <  2:112099/122‑3 (MQ=255)

                                    tCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGTGACGGCCCGCTGATTGCCCAACTGCGCCTTGAGCGTGAGTACCAGGTGGCCTGGTActc                                                                                                                                            <  2:106306/122‑3 (MQ=255)

                                    tCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGt                                                                                                                 >  7:166019/1‑149 (MQ=255)

                                      cgcgCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCCGCGACGGGGTGACAAGGTGc                                                                                                               >  3:348996/1‑149 (MQ=255)

                                       gcgcTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGcc                                                                                                              >  5:43919/1‑149 (MQ=255)

                                        cgcTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCg                                                                                                             <  3:358094/149‑1 (MQ=255)

                                        cgcTGAAAGAGCGCCCGGAACGGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCg                                                                                                             <  4:297925/149‑1 (MQ=255)

                                         gcTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCgg                                                                                                            <  3:235024/149‑1 (MQ=255)

                                          cTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGc                                                                                                           <  4:69260/149‑1 (MQ=255)

                                          cTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGc                                                                                                           <  4:43143/149‑1 (MQ=255)

                                          cTGAAAGAGCGCACGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGc                                                                                                           <  5:303595/149‑1 (MQ=255)

                                           tGAAAGAGCGCCCGGAACTGCGCCTCGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGc                                                                                                           >  4:202116/1‑148 (MQ=255)

                                            gAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGcc                                                                                                          >  8:179092/1‑148 (MQ=255)

                                             aaaGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCaa                                                                                                        >  2:320463/1‑149 (MQ=255)

                                             aaaGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCaa                                                                                                        >  5:130799/1‑149 (MQ=255)

                                                      ccGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGAt                                                                                               <  6:43919/149‑1 (MQ=255)

                                                        ggAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGc                                                                                             <  2:283787/149‑1 (MQ=255)

                                                           aCTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTTAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCtgg                                                                                          <  5:42061/149‑1 (MQ=255)

                                                             tGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGCCCACCTGGTACAAGATCCTGCAGAGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCtggtg                                                                                        >  5:227157/1‑149 (MQ=255)

                                                              gcgcCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGAGAAGGTGCCGGCCAATGCCTCGATGCtggtgg                                                                                       >  8:308485/1‑149 (MQ=255)

                                                               cgcgTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCTGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCtggtgg‑t                                                                                     <  7:190030/149‑1 (MQ=255)

                                                               cgcCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCtggtg                                                                                        <  7:281111/147‑1 (MQ=255)

                                                                 ccTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGTCCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCtggtgg‑tgg                                                                                   <  1:391547/149‑1 (MQ=255)

                                                                    ggAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGgac                                                                                 >  3:86516/1‑148 (MQ=255)

                                                                     gAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGgac                                                                                 <  7:179092/147‑1 (MQ=255)

                                                                      aaaTCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGgacga                                                                               >  2:271863/1‑148 (MQ=255)

                                                                       aaTCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACa                                                                             <  1:406180/149‑1 (MQ=255)

                                                                         tCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGt                                                                                                                         >  7:71374/1‑104 (MQ=255)

                                                                         tCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGt                                                                                                                         <  8:71374/104‑1 (MQ=255)

                                                                         tCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGc                                                                           <  8:121399/149‑1 (MQ=255)

                                                                         tCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGc                                                                           >  4:259271/1‑149 (MQ=255)

                                                                            aaGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGa                                                                         >  6:61153/1‑148 (MQ=255)

                                                                              ggCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACaa                                                                      <  3:20374/149‑1 (MQ=255)

                                                                               gCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGCTCCTGCAGCGACGGGGTGACAAGGTGCCGCCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAg                                                                     >  2:101066/1‑149 (MQ=255)

                                                                                  ccAGCGCCCAGTCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGccc                                                                  <  4:346820/149‑1 (MQ=255)

                                                                                     gcgcCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCcgcg                                                               <  3:419402/149‑1 (MQ=255)

                                                                                     gcgcCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCcgc                                                                >  3:387822/1‑148 (MQ=255)

                                                                                      cgcCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGa                                                              <  3:193342/149‑1 (MQ=255)

                                                                                        cccAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATg                                                            <  1:271863/149‑1 (MQ=255)

                                                                                        cccAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATg                                                            <  4:348996/149‑1 (MQ=255)

                                                                                         ccAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGc                                                           >  1:383477/1‑149 (MQ=255)

                                                                                           aGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTc                                                         <  8:378601/149‑1 (MQ=255)

                                                                                              ccAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGaa                                                      <  6:130799/149‑1 (MQ=255)

                                                                                                  cGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCa                                                  >  2:419936/1‑149 (MQ=255)

                                                                                                    aCGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCAtt                                                <  3:259271/149‑1 (MQ=255)

                                                                                                      ggCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTa                                              >  5:414743/1‑149 (MQ=255)

                                                                                                       gCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGTCCGCGATGCTCGAAGGCATTTAc                                             <  7:308485/149‑1 (MQ=255)

                                                                                                        cccGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTCCCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGCTGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCGCGAAGGCATTTAcc                                            <  2:327499/149‑1 (MQ=255)

                                                                                                               aTTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCg                                     >  4:53005/1‑149 (MQ=255)

                                                                                                                ttGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGCTGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTAccc                                      <  1:320463/148‑1 (MQ=255)

                                                                                                                   cccAACAGCGCCTGGAGCGTGAGTACCACGCCACCTGGTACAAGATCCTGCAGCGACTGTGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTg                                 <  8:366101/149‑1 (MQ=255)

                                                                                                                    ccAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTg                                 >  2:252268/1‑148 (MQ=255)

                                                                                                                      aaCAGCGCCTTGAGCGTGAGTACCTGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCTATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGaaa                              <  3:53005/149‑1 (MQ=255)

                                                                                                                         aGCGCCTTGAGCGTGAGTACCAGGCCCCCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCtggtg                                                                                        >  8:173418/1‑89 (MQ=255)

                                                                                                                           cgcCTTGAGCGGGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAAcagca                         <  4:38019/149‑1 (MQ=255)

                                                                                                                              cTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAgcc                      <  3:202116/149‑1 (MQ=255)

                                                                                                                                 gAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGGAACAGCAgccgc                    <  4:38140/148‑1 (MQ=255)

                                                                                                                                  aGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCt                  <  1:394727/149‑1 (MQ=255)

                                                                                                                                      tGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCAACAAGCCAGCGATGCTCGAAGGAATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGcc               >  1:140628/1‑148 (MQ=255)

                                                                                                                                       gAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCg              >  5:374987/1‑148 (MQ=255)

                                                                                                                                         gTACCAGGTGGCCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAgccgc                    <  5:303548/140‑1 (MQ=255)

                                                                                                                                         gTACCAGGTGGCCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAgccgc                    >  6:303548/1‑140 (MQ=255)

                                                                                                                                         gTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGAAt           <  6:414743/149‑1 (MQ=255)

                                                                                                                                          tACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGATTg          >  4:388291/1‑149 (MQ=255)

                                                                                                                                          tACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGAATg          >  7:102776/1‑149 (MQ=255)

                                                                                                                                            ccAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAg                                                     <  5:187776/104‑1 (MQ=255)

                                                                                                                                            ccAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAg                                                     >  6:187776/1‑104 (MQ=255)

                                                                                                                                            ccAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGGCGCCTGCCGAATggg        <  2:383477/149‑1 (MQ=255)

                                                                                                                                            ccAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGAATggg        <  1:419936/149‑1 (MQ=255)

                                                                                                                                               ggCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGAATGGGAg      >  5:143677/1‑148 (MQ=255)

                                                                                                                                                 ccACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCAGTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGAAAGTGAGCAg   >  8:69838/1‑149 (MQ=255)

                                                                                                                                                  cACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGAATGGGAGCAGt  >  3:284347/1‑149 (MQ=255)

AGCGGCGAGGCCCAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGAATGGGAGCAGT  >  NC_002947/5349939‑5350233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`