BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,350,086	A→G	5.5%	T854A (ACC→GCC)	PP_4704 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,350,086	0	A	G	5.5%	310.1 / 5.0	109	T854A (ACC→GCC)	PP_4704	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (48/54); new base G (3/3); total (52/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.75e-01

Only 100 of 110 total aligned reads displayed.

AGCGGCGAGGCCCAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGAATGGGAGCAGTT  >  NC_002947/5349939‑5350234

aGCGGCGAGGCCCAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCAc                                                                                                                                                      >  7:177357/1‑149 (MQ=255)

  ccgcgAGGCCCAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGCAACTGCGCCAGGAAATCGAAGGCACCAGAGCCCAGGCCAGCGACGGCCCGATCATAGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCt                                                                                                                                                    >  8:190030/3‑149 (MQ=255)

   ggcgAGGCCCAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTg                                                                                                                                                   <  2:347494/149‑1 (MQ=255)

    gcgAGGCCCAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTgg                                                                                                                                                  >  4:419402/1‑149 (MQ=255)

      gAGGCCCAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTa                                                                                                                                                <  7:24391/149‑1 (MQ=255)

      gAGGCCCAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTa                                                                                                                                                <  7:233885/149‑1 (MQ=255)

      gAGGCCCAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTa                                                                                                                                                >  4:20374/1‑149 (MQ=255)

         gCCCAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACaa                                                                                                                                             >  3:43143/1‑149 (MQ=255)

           ccAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGa                                                                                                                                           <  6:278994/149‑1 (MQ=255)

           ccAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGa                                                                                                                                           <  8:211858/149‑1 (MQ=255)

              gtcgtcCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACa                                                                                                                                              >  1:79675/1‑143 (MQ=255)

              gtcgtcCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACa                                                                                                                                              <  2:79675/143‑1 (MQ=255)

                 gtcCTTGGACAAGCTGACATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGTGTACCAGGCCACCTGGTACAAGATCCTg                                                                                                                                      <  1:246745/148‑1 (MQ=255)

                  tcCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCa                                                                                                                                    <  6:78021/149‑1 (MQ=255)

                     ttGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGc                                                                                                                                  >  7:121399/1‑148 (MQ=255)

                       ggACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCg                                                                                                                                 <  3:152433/147‑1 (MQ=255)

                        gACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGAc                                                                                                                               <  5:94071/148‑1 (MQ=255)

                         aCAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACgg                                                                                                                             >  2:81486/1‑149 (MQ=255)

                          cAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGt                                                                                                                                                 >  3:16127/1‑128 (MQ=255)

                          cAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGt                                                                                                                                                 <  4:16127/128‑1 (MQ=255)

                          cAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACggg                                                                                                                            <  5:404293/149‑1 (MQ=255)

                           aaGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACgggg                                                                                                                           >  5:114897/1‑149 (MQ=255)

                           aaGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACgggg                                                                                                                           >  3:38140/1‑149 (MQ=255)

                           aaGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACgggg                                                                                                                           >  7:378601/1‑149 (MQ=255)

                            aGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGt                                                                                                                          <  6:114897/149‑1 (MQ=255)

                               tGGCATCCGCGCTGAAAGAGCGCACGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGACCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGa                                                                                                                        >  8:11107/1‑148 (MQ=255)

                                  cATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGTGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAg                                                                                                                    <  1:333082/149‑1 (MQ=255)

                                   aTCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAgg                                                                                                                   >  4:193342/1‑149 (MQ=255)

                                   aTCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAgg                                                                                                                   >  7:71057/1‑149 (MQ=255)

                                    tCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGTGACGGCCCGCTGATTGCCCAACTGCGCCTTGAGCGTGAGTACCAGGTGGCCTGGTActc                                                                                                                                             >  1:112099/1‑120 (MQ=255)

                                    tCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGTGACGGCCCGCTGATTGCCCAACTGCGCCTTGAGCGTGAGTACCAGGTGGCCTGGTActc                                                                                                                                             >  1:106306/1‑120 (MQ=255)

                                    tCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGTGACGGCCCGCTGATTGCCCAACTGCGCCTTGAGCGTGAGTACCAGGTGGCCTGGTActc                                                                                                                                             <  2:106306/122‑3 (MQ=255)

                                    tCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGTGACGGCCCGCTGATTGCCCAACTGCGCCTTGAGCGTGAGTACCAGGTGGCCTGGTActc                                                                                                                                             <  2:112099/122‑3 (MQ=255)

                                    tCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGt                                                                                                                  >  7:166019/1‑149 (MQ=255)

                                      cgcgCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCCGCGACGGGGTGACAAGGTGc                                                                                                                >  3:348996/1‑149 (MQ=255)

                                       gcgcTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGcc                                                                                                               >  5:43919/1‑149 (MQ=255)

                                        cgcTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCg                                                                                                              <  3:358094/149‑1 (MQ=255)

                                        cgcTGAAAGAGCGCCCGGAACGGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCg                                                                                                              <  4:297925/149‑1 (MQ=255)

                                         gcTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCgg                                                                                                             <  3:235024/149‑1 (MQ=255)

                                          cTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGc                                                                                                            <  4:69260/149‑1 (MQ=255)

                                          cTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGc                                                                                                            <  4:43143/149‑1 (MQ=255)

                                          cTGAAAGAGCGCACGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGc                                                                                                            <  5:303595/149‑1 (MQ=255)

                                           tGAAAGAGCGCCCGGAACTGCGCCTCGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGc                                                                                                            >  4:202116/1‑148 (MQ=255)

                                            gAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGcc                                                                                                           >  8:179092/1‑148 (MQ=255)

                                             aaaGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCaa                                                                                                         >  5:130799/1‑149 (MQ=255)

                                             aaaGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCaa                                                                                                         >  2:320463/1‑149 (MQ=255)

                                                      ccGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGAt                                                                                                <  6:43919/149‑1 (MQ=255)

                                                        ggAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGc                                                                                              <  2:283787/149‑1 (MQ=255)

                                                           aCTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTTAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCtgg                                                                                           <  5:42061/149‑1 (MQ=255)

                                                             tGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCtggtg                                                                                         >  3:346820/1‑149 (MQ=255)

                                                             tGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGCCCACCTGGTACAAGATCCTGCAGAGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCtggtg                                                                                         >  5:227157/1‑149 (MQ=255)

                                                              gcgcCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGAGAAGGTGCCGGCCAATGCCTCGATGCtggtgg                                                                                        >  8:308485/1‑149 (MQ=255)

                                                               cgcgTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCTGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCtggtgg‑t                                                                                      <  7:190030/149‑1 (MQ=255)

                                                               cgcCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCtggtg                                                                                         <  7:281111/147‑1 (MQ=255)

                                                                 ccTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGTCCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCtggtgg‑tgg                                                                                    <  1:391547/149‑1 (MQ=255)

                                                                    ggAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGgac                                                                                  >  3:86516/1‑148 (MQ=255)

                                                                      aaaTCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGgacga                                                                                >  2:271863/1‑148 (MQ=255)

                                                                         tCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGt                                                                                                                          >  7:71374/1‑104 (MQ=255)

                                                                         tCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGt                                                                                                                          <  8:71374/104‑1 (MQ=255)

                                                                         tCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGc                                                                            >  4:259271/1‑149 (MQ=255)

                                                                            aaGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGa                                                                          >  6:61153/1‑148 (MQ=255)

                                                                               gCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGCTCCTGCAGCGACGGGGTGACAAGGTGCCGCCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAg                                                                      >  2:101066/1‑149 (MQ=255)

                                                                                  ccAGCGCCCAGTCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGccc                                                                   <  4:346820/149‑1 (MQ=255)

                                                                                     gcgcCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCcgcg                                                                <  3:419402/149‑1 (MQ=255)

                                                                                     gcgcCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCcgc                                                                 >  3:387822/1‑148 (MQ=255)

                                                                                      cgcCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGa                                                               <  3:193342/149‑1 (MQ=255)

                                                                                        cccAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATg                                                             <  1:271863/149‑1 (MQ=255)

                                                                                        cccAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATg                                                             <  4:348996/149‑1 (MQ=255)

                                                                                         ccAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGc                                                            >  1:383477/1‑149 (MQ=255)

                                                                                           aGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTc                                                          <  8:378601/149‑1 (MQ=255)

                                                                                              ccAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGaa                                                       <  6:130799/149‑1 (MQ=255)

                                                                                                  cGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCa                                                   >  2:419936/1‑149 (MQ=255)

                                                                                                    aCGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCAtt                                                 <  3:259271/149‑1 (MQ=255)

                                                                                                      ggCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTa                                               >  5:414743/1‑149 (MQ=255)

                                                                                                       gCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGTCCGCGATGCTCGAAGGCATTTAc                                              <  7:308485/149‑1 (MQ=255)

                                                                                                               aTTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCg                                      >  4:53005/1‑149 (MQ=255)

                                                                                                                ttGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGGCTGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTAccc                                       <  1:320463/148‑1 (MQ=255)

                                                                                                                  gCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCt                                   >  2:394727/1‑149 (MQ=255)

                                                                                                                   cccAACAGCGCCTGGAGCGTGAGTACCACGCCACCTGGTACAAGATCCTGCAGCGACTGTGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTg                                  <  8:366101/149‑1 (MQ=255)

                                                                                                                    ccAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTg                                  >  2:252268/1‑148 (MQ=255)

                                                                                                                         aGCGCCTTGAGCGTGAGTACCAGGCCCCCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCtggtg                                                                                         >  8:173418/1‑89 (MQ=255)

                                                                                                                           cgcCTTGAGCGGGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAAcagca                          <  4:38019/149‑1 (MQ=255)

                                                                                                                              cTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAgcc                       <  3:202116/149‑1 (MQ=255)

                                                                                                                                  aGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCt                   <  1:394727/149‑1 (MQ=255)

                                                                                                                                      tGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCAACAAGCCAGCGATGCTCGAAGGAATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGcc                >  1:140628/1‑148 (MQ=255)

                                                                                                                                       gAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCg               >  1:46964/1‑148 (MQ=255)

                                                                                                                                       gAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCg               >  5:374987/1‑148 (MQ=255)

                                                                                                                                         gTACCAGGTGGCCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAgccgc                     >  6:303548/1‑140 (MQ=255)

                                                                                                                                         gTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGAAt            <  6:414743/149‑1 (MQ=255)

                                                                                                                                          tACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGATTg           >  4:388291/1‑149 (MQ=255)

                                                                                                                                          tACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGAATg           >  7:102776/1‑149 (MQ=255)

                                                                                                                                            ccAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAg                                                      >  6:187776/1‑104 (MQ=255)

                                                                                                                                            ccAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAg                                                      <  5:187776/104‑1 (MQ=255)

                                                                                                                                            ccAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGGCGCCTGCCGAATggg         <  2:383477/149‑1 (MQ=255)

                                                                                                                                            ccAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGAATggg         <  1:419936/149‑1 (MQ=255)

                                                                                                                                               ggCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGAATGGGAg       >  5:143677/1‑148 (MQ=255)

                                                                                                                                                gCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGAATGGGAg       >  7:98330/1‑147 (MQ=255)

                                                                                                                                                 ccACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCAGTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGAAAGTGAGCAg    >  8:69838/1‑149 (MQ=255)

                                                                                                                                                  cACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGAATGGGAGCAGt   >  3:284347/1‑149 (MQ=255)

                                                                                                                                                   aCCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGAATGGGAGCAGtt  >  8:139230/1‑149 (MQ=255)

AGCGGCGAGGCCCAGTCGTCCTTGGACAAGCTGGCATCCGCGCTGAAAGAGCGCCCGGAACTGCGCCTGGAAATCGAAGGCACCAGCGCCCAGGCCAGCGACGGCCCGCTGATTGCCCAACAGCGCCTTGAGCGTGAGTACCAGGCCACCTGGTACAAGATCCTGCAGCGACGGGGTGACAAGGTGCCGGCCAATGCCTCGATGCTGGTGG‑TGGACGACAGCGACAAGCCCGCGATGCTCGAAGGCATTTACCGTACCCGCCTGAAACAGCAGCCGCCTGCCGAATGGGAGCAGTT  >  NC_002947/5349939‑5350234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`