BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	450,918	T→C	6.5%	noncoding (115/127 nt)	PP_mr05 →	RsmY ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	450,918	0	T	C	6.5%	316.2 / 9.1	107	noncoding (115/127 nt)	PP_mr05	RsmY ncRNA
Reads supporting (aligned to +/- strand): ref base T (51/49); new base C (4/3); total (55/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.20e-01

Only 100 of 107 total aligned reads displayed.

TAAGATTGGATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGAAATAC  >  NC_002947/450774‑451062

tAAGATTGGATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTcgg                                                                                                                                              >  1:199086/1‑149 (MQ=255)

  aGATTGGATCCACTGTTAATCTTTACCACATGGACGTGGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTcggcg                                                                                                                                            <  6:415218/149‑1 (MQ=255)

     ttGGATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCggg                                                                                                                                          <  1:153084/148‑1 (MQ=255)

     ttGGATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCggg                                                                                                                                          <  4:91974/148‑1 (MQ=255)

          tCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTc                                                                                                                                    >  7:59032/1‑149 (MQ=255)

          tCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTc                                                                                                                                    >  5:132328/1‑149 (MQ=255)

             aCTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAACCCCCGCCGAAGCGGGGTTTTcca                                                                                                                                  <  5:186645/149‑3 (MQ=255)

             aCTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCttt                                                                                                                                 >  6:372643/1‑149 (MQ=255)

             aCTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCttt                                                                                                                                 >  8:195625/1‑149 (MQ=255)

             aCTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCttt                                                                                                                                 >  1:325485/1‑149 (MQ=255)

              cTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTtg                                                                                                                                >  3:229693/1‑149 (MQ=255)

               tGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTtgc                                                                                                                               >  2:81414/1‑149 (MQ=255)

                   aaTCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTtgcgtgc                                                                                                                           >  6:136268/1‑149 (MQ=255)

                     tCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTgcgtgcgt                                                                                                                         >  3:357559/1‑149 (MQ=255)

                           ccACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGttttt                                                                                                                   <  7:239852/149‑1 (MQ=255)

                             acaTGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCa                                                                                                                 <  2:156491/149‑1 (MQ=255)

                              caTGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAg                                                                                                                >  5:193531/1‑149 (MQ=255)

                               aTGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGttttt                                                                                                                   >  8:140167/1‑145 (MQ=255)

                               aTGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCa                                                                                                                 <  7:140167/147‑1 (MQ=255)

                                 ggACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGc                                                                                                              >  5:232305/1‑148 (MQ=255)

                                  gACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGcc                                                                                                             <  5:136268/148‑1 (MQ=255)

                                  gACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGcc                                                                                                             <  1:81414/148‑1 (MQ=255)

                                        gcgcAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACAt                                                                                                      >  7:129795/1‑149 (MQ=255)

                                         cgcAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTGTTCAGGCCAGCACATc                                                                                                     >  1:152491/1‑149 (MQ=255)

                                          gcAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATc                                                                                                     >  8:335678/1‑148 (MQ=255)

                                              gAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCg                                                                                                >  4:73491/1‑149 (MQ=255)

                                              gAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCg                                                                                                <  2:135878/149‑1 (MQ=255)

                                              gAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCg                                                                                                <  8:33313/149‑1 (MQ=255)

                                              gAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCg                                                                                                <  7:23491/149‑1 (MQ=255)

                                               taGCGCAAAGGACAGATCAAGGTCGACGACTAAGGACCACCTGCCAGGATGGCGGGGCGATACTGATGTCACAGGGAAACAGTCTGGAAAA‑CCGCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGACAGCACATCCAGCg                                                                                                <  8:13132/147‑1 (MQ=255)

                                                 gcgcAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTg                                                                                             <  5:372643/149‑1 (MQ=255)

                                                   gcAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGAt                                                                                           >  5:157534/1‑149 (MQ=255)

                                                   gcAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGAt                                                                                           >  2:435544/1‑149 (MQ=255)

                                                     aaaGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGc                                                                                         >  1:81054/1‑149 (MQ=255)

                                                       aGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTc                                                                                       <  1:435544/149‑1 (MQ=255)

                                                         gACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTccc                                                                                     <  6:132328/149‑1 (MQ=255)

                                                          aCAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCa                                                                                    <  2:81054/149‑1 (MQ=255)

                                                            aGATCAAGGACGATGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTg                                                                                             <  5:313809/138‑1 (MQ=255)

                                                            aGATCAAGGACGATGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTg                                                                                             >  6:313809/1‑138 (MQ=255)

                                                             gATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCa                                                                                 <  8:115690/149‑1 (MQ=255)

                                                             gATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCa                                                                                 <  3:206175/149‑1 (MQ=255)

                                                               tCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAgg                                                                               <  6:154796/149‑1 (MQ=255)

                                                                cAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGGGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAgg                                                                               <  3:313882/148‑1 (MQ=255)

                                                                cAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGc                                                                              >  5:49243/1‑149 (MQ=255)

                                                                cAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGc                                                                              >  3:40778/1‑149 (MQ=255)

                                                                  aGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCTCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGa                                                                            <  3:286535/149‑1 (MQ=255)

                                                                  aGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATc                                                                                  <  3:157873/143‑1 (MQ=255)

                                                                  aGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATc                                                                                  >  4:157873/1‑143 (MQ=255)

                                                                  aGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGa                                                                            <  7:246435/149‑1 (MQ=255)

                                                                    gacgacgacGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGc                                                                          <  7:195625/149‑1 (MQ=255)

                                                                    gacgacgacGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGc                                                                          <  6:215514/149‑1 (MQ=255)

                                                                       gacgacgcAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGTGAAACAGTCTGGAAAA‑CCCAGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGcgtc                                                                       <  8:215254/149‑1 (MQ=255)

                                                                       gacgacgaAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGcgtc                                                                       >  3:247248/1‑149 (MQ=255)

                                                                        acgacgaAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGcgtcc                                                                      <  6:49243/149‑1 (MQ=255)

                                                                             gaAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGcgtcc                                                                      <  4:262487/144‑1 (MQ=255)

                                                                             gaAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGcgtcc                                                                      >  3:262487/1‑144 (MQ=255)

                                                                             gaAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCGTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGCTGCTCCCATCAGGCGAGCGTCCGtctc                                                                 >  5:256489/1‑149 (MQ=255)

                                                                              aaGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGGTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTctct                                                                <  6:83852/149‑1 (MQ=255)

                                                                                ggACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTGCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGCGCGTCCGGCTCTtc                                                              >  6:54995/1‑149 (MQ=255)

                                                                                   ccaccTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTtcctc                                                           <  3:73491/149‑1 (MQ=255)

                                                                                     accTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCa                                                         >  8:400036/1‑149 (MQ=255)

                                                                                     accTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCa                                                         <  5:181731/149‑1 (MQ=255)

                                                                                      ccTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAg                                                        <  2:325485/149‑1 (MQ=255)

                                                                                      ccTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAg                                                        >  7:63781/1‑149 (MQ=255)

                                                                                       cTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAg                                                        >  3:29264/1‑148 (MQ=255)

                                                                                        tGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGc                                                       >  6:73976/1‑148 (MQ=255)

                                                                                          ccAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTc                                                                                       >  8:343702/1‑114 (MQ=255)

                                                                                          ccAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTc                                                                                       <  7:343702/114‑1 (MQ=255)

                                                                                          ccAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAAc                                                    <  2:199086/149‑1 (MQ=255)

                                                                                          ccAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAAc                                                    >  4:273336/1‑149 (MQ=255)

                                                                                              gATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCCAGCGTCCGTCTCTTCCTCCAGCAACGTgc                                                >  7:373941/1‑149 (MQ=255)

                                                                                               aTGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTgcg                                               <  8:129795/149‑1 (MQ=255)

                                                                                                  gCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCgcag                                            <  4:40778/149‑1 (MQ=255)

                                                                                                   cGGGGCGATAAGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGACACCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTACTCCAGAAAAGTACgaag                                            >  6:326960/1‑148 (MQ=255)

                                                                                                               gATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACagag                               <  6:157534/149‑1 (MQ=255)

                                                                                                                 tGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGc                             <  4:229693/149‑1 (MQ=255)

                                                                                                                 tGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGc                             <  7:335678/149‑1 (MQ=255)

                                                                                                                     acaGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTctct                                                                >  3:240145/1‑110 (MQ=255)

                                                                                                                     acaGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTctct                                                                <  4:240145/110‑1 (MQ=255)

                                                                                                                     acaGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCctgc                         >  5:245323/1‑149 (MQ=255)

                                                                                                                     acaGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCctgc                         >  5:204345/1‑149 (MQ=255)

                                                                                                                       aGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCctgctg                       >  3:221702/1‑149 (MQ=255)

                                                                                                                       aGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCctgctg                       >  6:369539/1‑149 (MQ=255)

                                                                                                                         ggAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGgc                     >  7:42503/1‑149 (MQ=255)

                                                                                                                         ggAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGgc                     <  6:303447/149‑1 (MQ=255)

                                                                                                                          gAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCCGCACATCCAGCGGTGATGCTCCCATCAGGTGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGgcg                    >  2:299054/1‑149 (MQ=255)

                                                                                                                             aCAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGGGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTg                 <  4:357559/149‑1 (MQ=255)

                                                                                                                                gcaaagAAAA‑CCCCGCCGAAGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACagag                               <  4:328786/127‑1 (MQ=255)

                                                                                                                                gcaaagAAAA‑CCCCGCCGAAGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACagag                               >  3:328786/6‑132 (MQ=255)

                                                                                                                                 tCTGGAAAA‑CCCCGCTTCGGGGGGGTTTTCTTTGCGTGCGTGTTTTTCCGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCa             >  5:12963/1‑149 (MQ=255)

                                                                                                                                 tCTGGAAAA‑CCCCGCTTCGGCGGGGTTTACTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCa             >  1:26597/1‑149 (MQ=255)

                                                                                                                                   tGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTATTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGACCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCCCAGAGGCCTGCTGGCGCTGGGCATc           >  7:48303/1‑149 (MQ=255)

                                                                                                                                   aagAAAA‑CCCCGCCGAAGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATc           >  5:273882/3‑149 (MQ=255)

                                                                                                                                     gAAAA‑CCCCGCCGAAGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAg                                                                                                           >  4:89124/1‑51 (MQ=18)

                                                                                                                                     gAAAA‑CCCCGCCGAAGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAg                                                                                                           <  3:89124/51‑1 (MQ=18)

                                                                                                                                       aaa‑CCCCGCCGAAGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATcgcg        >  3:37564/1‑148 (MQ=255)

                                                                                                                                           ccccGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGAAAt    <  6:232305/149‑1 (MQ=255)

                                                                                                                                             ccGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTgcgc                                              <  2:120557/105‑1 (MQ=255)

                                                                                                                                             ccGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTgcgc                                              >  1:120557/1‑105 (MQ=255)

                                                                                                                                             ccGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGAAATAc  <  3:273336/149‑1 (MQ=255)

TAAGATTGGATCCACTGTTAATCTTTACCACATGGACGTTGCGCAGGAAGCGCAAAGGACAGATCAAGGACGACGACGAAGGACCACCTGCCAGGATGGCGGGGCGATACGGATGTCACAGGGAAACAGTCTGGAAAA‑CCCCGCTTCGGCGGGGTTTTCTTTGCGTGCGTGTTTTTCAGGCCAGCACATCCAGCGGTGATGCTCCCATCAGGCGAGCGTCCGTCTCTTCCTCCAGCAACGTGCGCAGCAGCTCCACAGAGGCCTGCTGGCGCTGGGCATCGCGAAATAC  >  NC_002947/450774‑451062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`