Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,361,072	A→T	10.8%	intergenic (‑79/+22)	PP_t49 ← / ← PP_t48	tRNA‑Met/tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,361,072	0	A	T	10.8%	227.4 / 14.9	83	intergenic (‑79/+22)	PP_t49/PP_t48	tRNA‑Met/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base A (35/39);  new base T (5/4);  total (40/43)
Fisher's exact test for biased strand distribution p-value = 7.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.17e-01

CTGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGCTCTTG  >  NC_002947/5360929‑5361215                                                                                                                                                |                                                                                                                                                gctgATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGCACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCgg                                                                                                                                            >  6:424628/4‑149 (MQ=255)   ggATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTTTAGCGGGc                                                                                                                                          >  6:261445/1‑149 (MQ=255)   ggATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGc                                                                                                                                          >  8:41253/1‑149 (MQ=255)    gATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCggg                                                                                                                                           <  3:395508/147‑1 (MQ=255)    gATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGct                                                                                                                                         >  2:246095/1‑149 (MQ=255)        tGAACCAACGACCTTCTGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTTTAGCGGGCtttc                                                                                                                                      <  6:121338/148‑1 (MQ=255)        tGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTTTAGCGGGCtttc                                                                                                                                      <  5:261445/148‑1 (MQ=255)        tGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTTTAGCGGGCtttc                                                                                                                                      <  4:275180/148‑1 (MQ=255)          aaCCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTTTAGCGGGCtttctt                                                                                                                                    <  1:312780/148‑3 (MQ=255)          aaCCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAg                                                                                                                                   >  2:12598/1‑149 (MQ=255)           aCCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGc                                                                                                                                  >  3:204403/1‑149 (MQ=255)            ccAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCt                                                                                                                                 >  1:117920/1‑149 (MQ=255)            ccAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCt                                                                                                                                 <  4:402745/149‑1 (MQ=255)            ccAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCt                                                                                                                                 <  2:361362/149‑1 (MQ=255)              aaCGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCta                                                                                                                                >  2:133580/1‑147 (MQ=255)               aCGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCttt                                                                                                                               >  8:56391/1‑148 (MQ=255)               aCGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTa                                                                                                                              <  8:51678/149‑1 (MQ=255)                  aCCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAAtt                                                                                                                           <  4:374408/149‑1 (MQ=255)                       cGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGt                                                                                                                        <  2:117920/147‑1 (MQ=255)                       cGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTa                                                                                                                       <  1:161851/148‑1 (MQ=255)                        gggTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTAc                                                                                                                      <  7:124762/148‑1 (MQ=255)                         ggTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTa                                                                                                                       >  4:50655/1‑146 (MQ=255)                         ggTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTa                                                                                                                       <  3:50655/146‑1 (MQ=255)                              tGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAg                                                                                                               >  5:194806/1‑149 (MQ=255)                               gAGCCCGACGAGCTACCAAGCTGCTCAACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAgg                                                                                                              >  1:10406/1‑149 (MQ=255)                                aGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATATGACCGATCCCTGTTGAGGGTCAATTCCAAACATTCACCTACAAGAAAGGGCGCTAAAGCGGTCTCTCAAGCTTTAATTGGTACCGAGAAgg                                                                                                              <  3:345950/148‑1 (MQ=255)                                aGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAggg                                                                                                             <  4:244709/149‑1 (MQ=255)                                aGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAgg                                                                                                              <  8:17882/148‑1 (MQ=255)                                aGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAgg                                                                                                              <  4:278808/148‑1 (MQ=255)                                aGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAgg                                                                                                              <  1:246095/148‑1 (MQ=255)                                 gCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTTGTGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAggg                                                                                                             <  3:92933/148‑1 (MQ=255)                                   ccGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGAc                                                                                                          <  1:12598/149‑1 (MQ=255)                                   ccGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGAc                                                                                                          <  5:141925/149‑1 (MQ=255)                                    cgacgaGCTACCAAGCTGCTCCACCCCTCGACAAAGCTGTGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATACAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACt                                                                                                         <  2:10406/149‑1 (MQ=255)                                    cgacgaGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACt                                                                                                         >  7:194884/1‑149 (MQ=255)                                     gacgaGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTc                                                                                                        >  4:122654/1‑149 (MQ=255)                                          gCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGCGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAAcc                                                                                                   <  4:173401/149‑1 (MQ=255)                                             aCCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAAc                                                                                                    >  4:237854/1‑145 (MQ=255)                                             aCCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAAc                                                                                                    <  3:237854/145‑1 (MQ=255)                                                aaGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTaca                                                                                             >  5:208907/1‑149 (MQ=255)                                                 aGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTacac                                                                                            <  7:56391/149‑1 (MQ=255)                                                      ctCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTAt                                                                                       >  4:160903/1‑149 (MQ=255)                                                          aCCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGc                                                                                   <  5:192512/149‑1 (MQ=255)                                                                    aaaGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAACGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGTGACTCGAACCCCTACACCCTATGGGCACAACCACCt                                                                         <  8:176744/149‑1 (MQ=255)                                                                     aaGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTc                                                                        <  4:204403/149‑1 (MQ=255)                                                                           gggCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAACGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGtt                                                                  >  7:153511/1‑149 (MQ=255)                                                                            ggCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTg                                                                 <  8:387525/149‑1 (MQ=255)                                                                             gCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGc                                                                >  4:347711/1‑149 (MQ=255)                                                                               aaaaGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGACCCACTACACCCGATGGGCCCCACCACCTCAAGGTTCCgt                                                              >  7:354835/1‑149 (MQ=255)                                                                               aaaaGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACCACCACCTCAAGGTTGctg                                                              >  8:179141/1‑147 (MQ=255)                                                                                      taAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTAc                                                        >  8:401373/1‑148 (MQ=255)                                                                                      taAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTAc                                                        >  5:193208/1‑148 (MQ=255)                                                                                       aaGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTAcc                                                       >  6:438689/1‑148 (MQ=255)                                                                                       aaGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTAcc                                                       >  4:156656/1‑148 (MQ=255)                                                                                          aCCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGTGGACTCGAACCACTACACCCTATGGGCACAAACACATCAAGGTTGCGTGTCTACCAAt                                                    >  5:131029/1‑148 (MQ=255)                                                                                           ccGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCgt                                                              >  2:396657/1‑137 (MQ=255)                                                                                           ccGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCgt                                                              <  1:391263/137‑1 (MQ=255)                                                                                           ccGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCgt                                                              >  2:391263/1‑137 (MQ=255)                                                                                           ccGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCgt                                                              <  1:396657/137‑1 (MQ=255)                                                                                            cGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTATTTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTcc                                                 <  2:157632/149‑1 (MQ=255)                                                                                            cGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTcc                                                 <  6:194806/149‑1 (MQ=255)                                                                                            cGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTc                                                  >  2:240191/1‑148 (MQ=255)                                                                                             gAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTcca                                                <  6:208907/149‑1 (MQ=255)                                                                                               aCCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTccacc                                              <  8:153511/149‑1 (MQ=255)                                                                                                   tGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCaccacc                                           >  2:82759/1‑148 (MQ=255)                                                                                                     ttGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCaccacc                                           <  1:82759/146‑1 (MQ=255)                                                                                                     ttGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTc                                         <  7:401373/148‑1 (MQ=255)                                                                                                          ggTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCaa                                    >  1:409184/1‑148 (MQ=255)                                                                                                          ggTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCaa                                    >  1:429291/1‑148 (MQ=255)                                                                                                               aaTCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAAtactac                              <  3:122654/149‑1 (MQ=255)                                                                                                                            aCCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCTCAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCg                 <  7:41253/149‑1 (MQ=255)                                                                                                                            aCCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCg                 >  3:187954/1‑149 (MQ=255)                                                                                                                              cTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGtt               <  4:187954/149‑1 (MQ=255)                                                                                                                               tACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTa              >  7:302720/1‑149 (MQ=255)                                                                                                                                  ttgagaGCCCGCTTTAGCGGGCTTTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACt            >  2:122422/6‑148 (MQ=255)                                                                                                                                  ttgagaGCCCGCTTTAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACtt           >  4:67265/6‑149 (MQ=255)                                                                                                                                  ttgagaGCCCGCTTTAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACt            >  1:409519/6‑148 (MQ=255)                                                                                                                                  ttgagaGCCCGCTTTAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTGGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACtt           >  1:416031/6‑149 (MQ=255)                                                                                                                                   aGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTc          <  2:429291/149‑1 (MQ=255)                                                                                                                                    gAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTAc             <  5:19363/145‑1 (MQ=255)                                                                                                                                    gAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTAc             >  6:19363/1‑145 (MQ=255)                                                                                                                                           cGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGCTCTTg  <  1:133580/149‑1 (MQ=255)                                                                                                                                           cGCTAAAGCGGGCTCTCAAGCTTTAAGTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGCTCtt   >  3:282775/1‑148 (MQ=255)                                                                                                                                                |                                                                                                                                                CTGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGCTCTTG  >  NC_002947/5360929‑5361215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑