Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,419,003 C→G 6.1% S23T (AGT→ACT)  PP_4758 ← MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,419,0030CG 6.1% 249.2 / 3.5 78S23T (AGT→ACT) PP_4758MFS transporter
Reads supporting (aligned to +/- strand):  ref base C (31/42);  new base G (3/2);  total (34/44)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.52e-01

AAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGGAGGAGCTTATTCGCAGTGAAGTTGTACGACAAGATCA  >  NC_002947/5418862‑5419151
                                                                                                                                             |                                                                                                                                                    
aaGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCaa                                                                                                                                               <  5:106023/149‑1 (MQ=255)
 aGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCAGTGCCGTGGCCaag                                                                                                                                              <  6:394183/149‑2 (MQ=255)
 aGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAACCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAAc                                                                                                                                              <  2:75285/149‑1 (MQ=255)
     aTAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGt                                                                                                                                          <  5:76420/149‑1 (MQ=255)
         gCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAAGATGATGACGAACAGCGGCAGGTCGTGGGGCTTGACCTTGGCCACGGCGCTGGCGCACGCGTCGcc                                                                                                                                      >  7:307572/1‑149 (MQ=255)
         gCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGcc                                                                                                                                      <  4:123074/149‑1 (MQ=255)
         gCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGcc                                                                                                                                      <  3:155761/149‑1 (MQ=255)
          ccGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCt                                                                                                                                     <  1:249753/149‑1 (MQ=255)
          ccGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCt                                                                                                                                     <  7:254741/149‑1 (MQ=255)
            gCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTc                                                                                                                                   <  3:85835/149‑1 (MQ=255)
             cAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCa                                                                                                                                  <  3:398831/149‑1 (MQ=255)
                     aTGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCgg                                                                                                                            <  1:423122/147‑1 (MQ=255)
                          cAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACg                                                                                                                     <  8:35977/149‑1 (MQ=255)
                          cAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACg                                                                                                                     >  8:160554/1‑149 (MQ=255)
                              tCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGc                                                                                                                 >  4:417065/1‑149 (MQ=255)
                               cGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGc                                                                                                                 >  3:391692/1‑148 (MQ=255)
                                gCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGc                                                                                                                 >  8:322765/1‑147 (MQ=255)
                                gCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGATAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACTGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGc                                                                                                                 >  1:91428/1‑147 (MQ=255)
                                 cTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGGCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAg                                                                                                              <  2:131058/149‑1 (MQ=255)
                                  tttCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAgg                                                                                                             >  6:295956/1‑149 (MQ=255)
                                  ttgacAGGTGCGGGCGGAAGAAGCCAATGTTGACGCGGTCGATGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAgg                                                                                                             <  4:298863/145‑1 (MQ=255)
                                     ccAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGgtg                                                                                                          >  7:110381/1‑149 (MQ=255)
                                      cAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGgtg                                                                                                          <  8:110381/148‑1 (MQ=255)
                                            cgggcggACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCa                                                                                                   <  3:417065/149‑1 (MQ=255)
                                                 ggACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGc                                                                                              >  6:59060/1‑149 (MQ=255)
                                                   aCGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCggg                                                                                                                           <  7:87560/118‑1 (MQ=255)
                                                   aCGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCggg                                                                                                                           >  8:87560/1‑118 (MQ=255)
                                                        gCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGAtctc                                                                                       <  5:295956/149‑1 (MQ=255)
                                                         ccAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTcc                                                                                      <  3:348073/149‑1 (MQ=255)
                                                          cAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGAtct                                                                                        >  1:340604/1‑146 (MQ=255)
                                                          cAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGAtct                                                                                        <  2:340604/146‑1 (MQ=255)
                                                          cAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTccc                                                                                     >  4:277470/1‑149 (MQ=255)
                                                           aaTGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCa                                                                                    >  8:257878/1‑149 (MQ=255)
                                                                   cgcgGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTCGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGt                                                                            >  8:334086/1‑149 (MQ=255)
                                                                    gcgGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGtt                                                                           >  4:132624/1‑149 (MQ=255)
                                                                     cgGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGttt                                                                          <  7:257878/149‑1 (MQ=255)
                                                                        tCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTtg                                                                       >  5:8810/1‑149 (MQ=255)
                                                                          gAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTtgtg                                                                     <  3:326738/149‑1 (MQ=255)
                                                                          gAGGTAGTTGACGATGAACATGATGAAGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTtgtg                                                                     >  6:343014/1‑149 (MQ=255)
                                                                                gTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGcgggtc                                                               >  2:177004/1‑149 (MQ=255)
                                                                                   gACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCTTCGCCTTAAGCCCGGGCGGACGCAGCGAGGTTTGCGTTCAGTTGCGGAGCTCCCCATAATTGTTTTTGTGcggtgcggt                                                            >  4:256071/1‑143 (MQ=255)
                                                                                   gACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGcgggtcggg                                                            >  4:244729/1‑149 (MQ=255)
                                                                                   gACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGcgggtcgg                                                             <  7:40648/148‑1 (MQ=255)
                                                                                    aCGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGTATCTCCCACTAATTGTTTTTGTGcgggtcggg                                                            <  1:51850/148‑1 (MQ=255)
                                                                                    aCGATGAACATGATGACGAACAGCGGCAGGACGGGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGcgggtcggg                                                            <  7:322765/148‑1 (MQ=255)
                                                                                          aaCATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCt                                                      >  7:86405/1‑148 (MQ=255)
                                                                                          aaCATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCt                                                      <  6:8810/148‑1 (MQ=255)
                                                                                            cATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGAc                                                   <  7:334086/149‑1 (MQ=255)
                                                                                                atgaCGAACAGCGGCAGGTCGTGGCGCTTGACCTTGGCCACGGCAGTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGTGTTTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATgg                                               <  7:84630/149‑1 (MQ=255)
                                                                                                  gaCGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATgg                                               >  3:224100/1‑147 (MQ=255)
                                                                                                   aCGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCa                                             <  8:86405/148‑1 (MQ=255)
                                                                                                       aCAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTggg                                        <  4:391692/149‑1 (MQ=255)
                                                                                                       aCAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTggg                                        >  7:208528/1‑149 (MQ=255)
                                                                                                        cAGCCGCAGGACGAGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCCCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCCGATCTCCCACAAATTGTTTTAGTGCGGGACGCGAGGTCTGACATGGCAGTgcg                                        >  4:193776/1‑146 (MQ=255)
                                                                                                          gCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGggag                                     <  7:160554/149‑1 (MQ=255)
                                                                                                              cAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGgaggag                                  <  3:59312/148‑1 (MQ=255)
                                                                                                              cAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGgaggag                                  >  4:59312/1‑148 (MQ=255)
                                                                                                                 gACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGTGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGGAGGAGCTTa                              >  3:310305/1‑149 (MQ=255)
                                                                                                                 gACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGAtc                                                                                         <  1:185638/90‑1 (MQ=255)
                                                                                                                 gACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGAtc                                                                                         >  2:185638/1‑90 (MQ=255)
                                                                                                                    gTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGGAGGAGCTTATTc                           <  8:208528/149‑1 (MQ=255)
                                                                                                                    gTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGACGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGGAGGAGCTTATTc                           <  7:283326/149‑1 (MQ=255)
                                                                                                                     tGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCCGACATGGCAGTGGGGAGGAGCTTATTCg                          <  3:132624/149‑1 (MQ=255)
                                                                                                                      ggCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGGAGGAGCTTATTCGc                         <  3:277470/149‑1 (MQ=255)
                                                                                                                       gcggTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCTGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGTCATGGCAGTGGTGAGGAGCTTATTCGCa                        <  3:45885/149‑1 (MQ=255)
                                                                                                                        cggttGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGCAGGAGCTTATTCGCAg                       >  5:91680/4‑149 (MQ=255)
                                                                                                                          cTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGGAGGAGCTTTTTAGCAGt                      >  6:303496/1‑148 (MQ=255)
                                                                                                                             gACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGGAGGAGCTTATTCGCAGTGaa                   <  2:91428/148‑1 (MQ=255)
                                                                                                                              gcgttGGCCAGTGCCGTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGGAGGAGCTTATTGGCAGTGAAGt                 >  3:342710/4‑149 (MQ=255)
                                                                                                                               ccTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGGAGGAGCTTATTCGCAGTGAAGtt                <  5:59060/149‑1 (MQ=255)
                                                                                                                                 ttGGCCAGTGCCGTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGGAGGAGCTTATTCGCAGTGAAGTTg               >  3:177128/1‑148 (MQ=255)
                                                                                                                                 ttGGCCAGTGCCGTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGGAGGAGCTTATTCGCAGTGAAGTTg               >  1:266651/1‑148 (MQ=255)
                                                                                                                                     ccACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTTTGCGGGTCGGGTTGTCTGACATGGCAGTGTGGAGGAGCTTATTCGCAGTGAAGTTGTActa          <  5:303496/149‑3 (MQ=255)
                                                                                                                                      cACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGGAGGAGCTTATTCGCAGTGAAGTTGTACGAc         <  3:244729/149‑1 (MQ=255)
                                                                                                                                         ggCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGGAGGAGCTTATTCGCAGTGAAGTTGTACGACAAg      <  1:177004/149‑1 (MQ=255)
                                                                                                                                            aCTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGGAGGAGCTTATTCGCAGTGAAGTTGTACGACAAGAt    >  7:91978/1‑148 (MQ=255)
                                                                                                                                            aCTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGGAGGAGCTTATTCGCAGTGAAGTTGTACGACAAGAt    >  8:255345/1‑148 (MQ=255)
                                                                                                                                            aCTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGGAGGAGCTTATTCGCAGTGAAGTTGTACGACAAGAt    <  8:91978/148‑1 (MQ=255)
                                                                                                                                             cTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGGAGGAGCTTATTCGCAGTGAAGTTGTACGACAAGATCa  >  8:343452/1‑149 (MQ=255)
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AAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGGAGGAGCTTATTCGCAGTGAAGTTGTACGACAAGATCA  >  NC_002947/5418862‑5419151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑