BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,503,135	C→A	5.5%	L29M (CTG→ATG) ‡	oprC →	copper receptor OprC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,503,135	0	C	A	5.5%	340.2 / 5.6	106	L29M (CTG→ATG) ‡	oprC	copper receptor OprC
Reads supporting (aligned to +/- strand): ref base C (52/47); new base A (3/3); total (55/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

CAACATTTTGCGCACGAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGG  >  NC_002947/5502987‑5503280

caacaTTTTGCGCACGAAGCCAGGAGGCTTAGCGCAAACTCATGACTGAACGACTGGAATCATTATGTCCGGCTGC‑ACCCCGGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCCCTGCTCGCGCCCATGGccc                                                                                                                                                   >  1:258633/1‑149 (MQ=255)

 aacaTTTTGCGCACGAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCt                                                                                                                                                  >  2:158879/1‑149 (MQ=255)

          cgcACGAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGccgccg                                                                                                                                          <  7:211493/148‑1 (MQ=255)

             aCGAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGccc                                                                                                                                      >  1:51710/1‑149 (MQ=255)

               gAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTg                                                                                                                                    <  4:318047/149‑1 (MQ=255)

               gAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCATGTTTTTGCCTAGTCCCTGCGTGGGACCAACGCCCCCCAGAGCGGCTCGCTGATCGCGCCCATGGCCCTGGCCGCCGccccag                                                                                                                                    >  7:151243/1‑147 (MQ=255)

                caGCCAGGAGGCTTCGCGCTAACTCATGACTGCTCGACTGGAATCATTATGTCCGGCGGC‑ACCCCTGTTTGTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCGGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTgg                                                                                                                                   <  3:170818/148‑1 (MQ=255)

                 aGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGc                                                                                                                                  >  8:384377/1‑149 (MQ=255)

                 aGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGc                                                                                                                                  <  1:158879/149‑1 (MQ=255)

                 aGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGc                                                                                                                                  <  7:7912/149‑1 (MQ=255)

                  gCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGcc                                                                                                                                 <  2:258633/149‑1 (MQ=255)

                  gCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGc                                                                                                                                  >  2:149457/1‑148 (MQ=255)

                  gCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGc                                                                                                                                  >  6:216495/1‑148 (MQ=255)

                  gCCAGGAGGCTTCGCGCTAACCCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGcc                                                                                                                                 <  2:134869/149‑1 (MQ=255)

                     aggaggCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCAt                                                                                                                               >  3:83448/1‑148 (MQ=255)

                       gaggCTTCGTGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCACTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATg                                                                                                                              >  8:359855/1‑147 (MQ=255)

                       gaggCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGaa                                                                                                                            <  7:411576/149‑1 (MQ=255)

                       gaggCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGaa                                                                                                                            <  2:51710/149‑1 (MQ=255)

                          gCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCAGGGCCGGCGGCCAGGGCCATggg                                                                                                                            <  4:21069/146‑3 (MQ=255)

                          gCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCAGGGCCGGCGGCCAGGGCCATggg                                                                                                                            >  3:21069/1‑144 (MQ=255)

                                 gcTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGc                                                                                                                  <  1:99819/149‑1 (MQ=255)

                                  cTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCa                                                                                                                 >  5:268318/1‑149 (MQ=255)

                                       tCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCacgacg                                                                                                            >  3:363061/1‑149 (MQ=255)

                                         aTGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTCCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCa                                                                                                          >  8:298210/1‑149 (MQ=255)

                                           gACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCAcc                                                                                                        >  2:57589/1‑149 (MQ=255)

                                             cTGATCGACTGGAATCATTATGTCCGGCTGCAACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCAccc                                                                                                       >  1:265751/1‑149 (MQ=255)

                                                aTCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGt                                                                                                   >  7:125374/1‑149 (MQ=255)

                                                     cTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGc                                                                                              >  3:73626/1‑149 (MQ=255)

                                                        gAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCTTGAAGACCATGTGCACGACGCACCCGAGTTGAGccc                                                                                            >  4:207057/1‑148 (MQ=255)

                                                        gAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGcc                                                                                             <  2:265751/147‑1 (MQ=255)

                                                        gAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCg                                                                                           >  7:114112/1‑149 (MQ=255)

                                                        gAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCCAGTTGAGCCCg                                                                                           >  3:337278/1‑149 (MQ=255)

                                                         aaTCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGAACGACGCACCCGAGTTAAGACCGa                                                                                          >  8:402379/1‑149 (MQ=255)

                                                          aTCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGAc                                                                                         >  5:258519/1‑149 (MQ=255)

                                                           tCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACg                                                                                        >  8:326635/1‑149 (MQ=255)

                                                           tCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACg                                                                                        >  8:370347/1‑149 (MQ=255)

                                                             aTTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGt                                                                                      <  7:298210/149‑1 (MQ=255)

                                                              ttATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTg                                                                                     <  7:402379/149‑1 (MQ=255)

                                                                 tGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCGCTGTGCGGCTCGCTGCTCGCGCCCATGGCGCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGAGGGTGATc                                                                                  <  6:216752/149‑1 (MQ=255)

                                                                 tGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGAt                                                                                   >  4:293353/1‑148 (MQ=255)

                                                                   tCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCa                                                                                 <  3:293353/148‑1 (MQ=255)

                                                                        cTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCAccgccg                                                                           >  7:86476/1‑149 (MQ=255)

                                                                         tGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGCCCGCCGGCCCTGGCCAGGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGt                                                                          >  5:154614/1‑149 (MQ=255)

                                                                          gC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTg                                                                         >  7:132471/1‑149 (MQ=255)

                                                                               cccTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGccc                                                                     >  8:150497/1‑149 (MQ=255)

                                                                                ccTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGcccc                                                                    <  4:363061/149‑1 (MQ=255)

                                                                                ccTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGcccc                                                                    <  8:125374/149‑1 (MQ=255)

                                                                                 cTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGcccc                                                                    >  7:264030/1‑148 (MQ=255)

                                                                                 cTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGcccc                                                                    >  5:90502/1‑148 (MQ=255)

                                                                                    tttttGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGt                                                                >  7:260297/1‑149 (MQ=255)

                                                                                    tttttGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGt                                                                >  1:355125/1‑149 (MQ=255)

                                                                                          ccTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTgg                                                                                                                                                >  1:110858/1‑63 (MQ=255)

                                                                                          ccTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTgg                                                                                                                                                <  2:110858/63‑1 (MQ=255)

                                                                                          ccTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCCCCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTcgccg                                                          >  5:37030/1‑149 (MQ=255)

                                                                                          ccTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGCCCCCGAGTTcgccg                                                          <  7:370347/149‑1 (MQ=255)

                                                                                           cTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTcgccgc                                                         >  8:212015/1‑149 (MQ=255)

                                                                                           cTTGTCCCTGCGTGGGACCAGCGCCGCCCTGTTCGGATCGATTCTCCCGCCCATGGCCCTGGCCGCCGGCCCTGTCCATGAAGACCATGTGAACGACGCACCCGAGTTGAGCCCTACGGTGATCACCGCCGTGGCCCCGAGTTcgccgc                                                         <  6:304060/149‑1 (MQ=255)

                                                                                              gTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTg                                                       <  4:73626/148‑1 (MQ=255)

                                                                                                 ccTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACtg                                                   >  6:322834/1‑149 (MQ=255)

                                                                                                  cTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACtgt                                                  >  1:402979/1‑149 (MQ=255)

                                                                                                   tGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTcgc                                                            >  6:184072/1‑138 (MQ=255)

                                                                                                   tGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTcgc                                                            <  5:184072/138‑1 (MQ=255)

                                                                                                   tGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACtgtg                                                 >  8:212353/1‑149 (MQ=255)

                                                                                                     cGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACtgtg                                                 <  6:90502/147‑1 (MQ=255)

                                                                                                           aCCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCaa                                         >  3:283361/1‑149 (MQ=255)

                                                                                                            ccATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAAc                                        >  7:408816/1‑149 (MQ=255)

                                                                                                             cATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAAcc                                       >  6:118242/1‑149 (MQ=255)

                                                                                                              aTCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAAccc                                      <  7:212015/149‑1 (MQ=255)

                                                                                                                cgccgcCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCg                                     >  7:38518/1‑148 (MQ=255)

                                                                                                                 gccgccCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGaa                                   <  8:38518/149‑1 (MQ=255)

                                                                                                                 gccgccCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGa                                    <  5:322834/148‑1 (MQ=255)

                                                                                                                 gccgccCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGa                                    <  3:422987/148‑1 (MQ=255)

                                                                                                                 gccgccCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGCGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGa                                    <  1:149457/148‑1 (MQ=255)

                                                                                                                  ccgccCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGaaa                                  >  8:276677/1‑149 (MQ=255)

                                                                                                                   cgccCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAg                                 <  5:130251/149‑1 (MQ=255)

                                                                                                                     cccTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCTTGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGAc                               <  3:207057/149‑1 (MQ=255)

                                                                                                                      ccTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGAcc                              <  7:406808/149‑1 (MQ=255)

                                                                                                                         gtgCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCcgc                           <  2:402979/149‑1 (MQ=255)

                                                                                                                          tgCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCcgcg                          <  7:276677/149‑1 (MQ=255)

                                                                                                                             ggCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCgcca                       <  5:39476/149‑1 (MQ=255)

                                                                                                                              gCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCCTGAAGACCATGTGCACGGCGCACCCGAGTTGAGCCCGCCGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCgccag                      >  5:302898/1‑149 (MQ=255)

                                                                                                                               cTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCgccagc                     <  8:132471/149‑1 (MQ=255)

                                                                                                                               cTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCgccagc                     <  5:118242/149‑1 (MQ=255)

                                                                                                                                  gctgctCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAAcc                                       >  3:404298/1‑128 (MQ=255)

                                                                                                                                  gctgctCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAAcc                                       <  4:404298/128‑1 (MQ=255)

                                                                                                                                   ctgctCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGt                 <  7:359855/149‑1 (MQ=255)

                                                                                                                                   ctgctCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGt                 <  7:326635/149‑1 (MQ=255)

                                                                                                                                        cgcgCCCATGGCCCTGGCCGCCCGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCACCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCgg            >  7:64025/1‑149 (MQ=255)

                                                                                                                                         gcgcCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCg             <  8:260297/147‑1 (MQ=255)

                                                                                                                                         gcgcCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGc           >  1:101487/1‑149 (MQ=255)

                                                                                                                                         gcgcCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGc           <  8:264030/149‑1 (MQ=255)

                                                                                                                                          tcttcATGGCCAGGGCCGGCGGCCAGGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGa                                    >  4:37885/5‑123 (MQ=38)

                                                                                                                                          tcttcATGGCCAGGGCCGGCGGCCAGGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGa                                    <  3:37885/119‑1 (MQ=38)

                                                                                                                                           gcCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGc           >  1:402239/1‑147 (MQ=255)

                                                                                                                                           gcCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCa         <  1:57589/149‑1 (MQ=255)

                                                                                                                                             ccATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGCCCCGCGCCAGCCAGTGCCGGCCAGc       >  1:250305/1‑149 (MQ=255)

                                                                                                                                                tcgCCCGGTCCGCCGGCCCTGTCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGGCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGAc    <  6:302898/147‑1 (MQ=255)

                                                                                                                                                tGGCCAGGGCCGGCGGCCAGGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCgccagc                     >  5:349934/1‑132 (MQ=255)

                                                                                                                                                  gcgcTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGTTCACCGCCGTGGCGCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACg   <  6:258519/145‑1 (MQ=255)

                                                                                                                                                  gCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACgg  <  5:177882/149‑1 (MQ=255)

CAACATTTTGCGCACGAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGG  >  NC_002947/5502987‑5503280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`