BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,503,136	T→G	6.0%	L29R (CTG→CGG) ‡	oprC →	copper receptor OprC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,503,136	0	T	G	6.0%	336.1 / 7.5	106	L29R (CTG→CGG) ‡	oprC	copper receptor OprC
Reads supporting (aligned to +/- strand): ref base T (51/48); new base G (3/4); total (54/52)
Fisher's exact test for biased strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

AACATTTTGCGCACGAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGGCGCC  >  NC_002947/5502988‑5503284

aacaTTTTGCGCACGAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCt                                                                                                                                                      >  2:158879/1‑149 (MQ=255)

         cgcACGAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGccgccg                                                                                                                                              <  7:211493/148‑1 (MQ=255)

            aCGAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGccc                                                                                                                                          >  1:51710/1‑149 (MQ=255)

              gAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTg                                                                                                                                        <  4:318047/149‑1 (MQ=255)

              gAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCATGTTTTTGCCTAGTCCCTGCGTGGGACCAACGCCCCCCAGAGCGGCTCGCTGATCGCGCCCATGGCCCTGGCCGCCGccccag                                                                                                                                        >  7:151243/1‑147 (MQ=255)

               caGCCAGGAGGCTTCGCGCTAACTCATGACTGCTCGACTGGAATCATTATGTCCGGCGGC‑ACCCCTGTTTGTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCGGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTgg                                                                                                                                       <  3:170818/148‑1 (MQ=255)

                aGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGc                                                                                                                                      <  7:7912/149‑1 (MQ=255)

                aGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGc                                                                                                                                      >  8:384377/1‑149 (MQ=255)

                aGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGc                                                                                                                                      <  1:158879/149‑1 (MQ=255)

                 gCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGcc                                                                                                                                     <  2:258633/149‑1 (MQ=255)

                 gCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGc                                                                                                                                      >  6:216495/1‑148 (MQ=255)

                 gCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGc                                                                                                                                      >  2:149457/1‑148 (MQ=255)

                 gCCAGGAGGCTTCGCGCTAACCCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGcc                                                                                                                                     <  2:134869/149‑1 (MQ=255)

                    aggaggCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCAt                                                                                                                                   >  3:83448/1‑148 (MQ=255)

                      gaggCTTCGTGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCACTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATg                                                                                                                                  >  8:359855/1‑147 (MQ=255)

                      gaggCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGaa                                                                                                                                <  7:411576/149‑1 (MQ=255)

                      gaggCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGaa                                                                                                                                <  2:51710/149‑1 (MQ=255)

                         gCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCAGGGCCGGCGGCCAGGGCCATggg                                                                                                                                <  4:21069/146‑3 (MQ=255)

                         gCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCAGGGCCGGCGGCCAGGGCCATggg                                                                                                                                >  3:21069/1‑144 (MQ=255)

                                gcTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGc                                                                                                                      <  1:99819/149‑1 (MQ=255)

                                 cTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCa                                                                                                                     >  5:268318/1‑149 (MQ=255)

                                      tCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCacgacg                                                                                                                >  3:363061/1‑149 (MQ=255)

                                        aTGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTCCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCa                                                                                                              >  8:298210/1‑149 (MQ=255)

                                          gACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCAcc                                                                                                            >  2:57589/1‑149 (MQ=255)

                                            cTGATCGACTGGAATCATTATGTCCGGCTGCAACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCAccc                                                                                                           >  1:265751/1‑149 (MQ=255)

                                               aTCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGt                                                                                                       >  7:125374/1‑149 (MQ=255)

                                                    cTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGc                                                                                                  >  3:73626/1‑149 (MQ=255)

                                                       gAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCTTGAAGACCATGTGCACGACGCACCCGAGTTGAGccc                                                                                                >  4:207057/1‑148 (MQ=255)

                                                       gAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGcc                                                                                                 <  2:265751/147‑1 (MQ=255)

                                                       gAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCg                                                                                               >  7:114112/1‑149 (MQ=255)

                                                       gAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCCAGTTGAGCCCg                                                                                               >  3:337278/1‑149 (MQ=255)

                                                        aaTCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGAACGACGCACCCGAGTTAAGACCGa                                                                                              >  8:402379/1‑149 (MQ=255)

                                                         aTCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGAc                                                                                             >  5:258519/1‑149 (MQ=255)

                                                          tCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACg                                                                                            >  8:370347/1‑149 (MQ=255)

                                                          tCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACg                                                                                            >  8:326635/1‑149 (MQ=255)

                                                            aTTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGt                                                                                          <  7:298210/149‑1 (MQ=255)

                                                             ttATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTg                                                                                         <  7:402379/149‑1 (MQ=255)

                                                                tGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCGCTGTGCGGCTCGCTGCTCGCGCCCATGGCGCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGAGGGTGATc                                                                                      <  6:216752/149‑1 (MQ=255)

                                                                tGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGAt                                                                                       >  4:293353/1‑148 (MQ=255)

                                                                  tCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCa                                                                                     <  3:293353/148‑1 (MQ=255)

                                                                       cTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCAccgccg                                                                               >  7:86476/1‑149 (MQ=255)

                                                                        tGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGCCCGCCGGCCCTGGCCAGGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGt                                                                              >  5:154614/1‑149 (MQ=255)

                                                                         gC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTg                                                                             >  7:132471/1‑149 (MQ=255)

                                                                              cccTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGccc                                                                         >  8:150497/1‑149 (MQ=255)

                                                                               ccTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGcccc                                                                        <  8:125374/149‑1 (MQ=255)

                                                                               ccTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGcccc                                                                        <  4:363061/149‑1 (MQ=255)

                                                                                cTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGcccc                                                                        >  7:264030/1‑148 (MQ=255)

                                                                                cTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGcccc                                                                        >  5:90502/1‑148 (MQ=255)

                                                                                   tttttGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGt                                                                    >  1:355125/1‑149 (MQ=255)

                                                                                   tttttGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGt                                                                    >  7:260297/1‑149 (MQ=255)

                                                                                         ccTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTgg                                                                                                                                                    >  1:110858/1‑63 (MQ=255)

                                                                                         ccTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTgg                                                                                                                                                    <  2:110858/63‑1 (MQ=255)

                                                                                         ccTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCCCCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTcgccg                                                              >  5:37030/1‑149 (MQ=255)

                                                                                         ccTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGCCCCCGAGTTcgccg                                                              <  7:370347/149‑1 (MQ=255)

                                                                                          cTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTcgccgc                                                             >  8:212015/1‑149 (MQ=255)

                                                                                          cTTGTCCCTGCGTGGGACCAGCGCCGCCCTGTTCGGATCGATTCTCCCGCCCATGGCCCTGGCCGCCGGCCCTGTCCATGAAGACCATGTGAACGACGCACCCGAGTTGAGCCCTACGGTGATCACCGCCGTGGCCCCGAGTTcgccgc                                                             <  6:304060/149‑1 (MQ=255)

                                                                                             gTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTg                                                           <  4:73626/148‑1 (MQ=255)

                                                                                                ccTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACtg                                                       >  6:322834/1‑149 (MQ=255)

                                                                                                 cTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACtgt                                                      >  1:402979/1‑149 (MQ=255)

                                                                                                  tGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTcgc                                                                >  6:184072/1‑138 (MQ=255)

                                                                                                  tGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTcgc                                                                <  5:184072/138‑1 (MQ=255)

                                                                                                  tGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACtgtg                                                     >  8:212353/1‑149 (MQ=255)

                                                                                                    cGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACtgtg                                                     <  6:90502/147‑1 (MQ=255)

                                                                                                          aCCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCaa                                             >  3:283361/1‑149 (MQ=255)

                                                                                                           ccATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAAc                                            >  7:408816/1‑149 (MQ=255)

                                                                                                            cATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAAcc                                           >  6:118242/1‑149 (MQ=255)

                                                                                                             aTCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAAccc                                          <  7:212015/149‑1 (MQ=255)

                                                                                                               cgccgcCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCg                                         >  7:38518/1‑148 (MQ=255)

                                                                                                                gccgccCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGaa                                       <  8:38518/149‑1 (MQ=255)

                                                                                                                gccgccCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGa                                        <  5:322834/148‑1 (MQ=255)

                                                                                                                gccgccCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGa                                        <  3:422987/148‑1 (MQ=255)

                                                                                                                gccgccCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGCGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGa                                        <  1:149457/148‑1 (MQ=255)

                                                                                                                 ccgccCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGaaa                                      >  8:276677/1‑149 (MQ=255)

                                                                                                                  cgccCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAg                                     <  5:130251/149‑1 (MQ=255)

                                                                                                                    cccTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCTTGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGAc                                   <  3:207057/149‑1 (MQ=255)

                                                                                                                     ccTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGAcc                                  <  7:406808/149‑1 (MQ=255)

                                                                                                                        gtgCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCcgc                               <  2:402979/149‑1 (MQ=255)

                                                                                                                         tgCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCcgcg                              <  7:276677/149‑1 (MQ=255)

                                                                                                                            ggCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCgcca                           <  5:39476/149‑1 (MQ=255)

                                                                                                                             gCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCCTGAAGACCATGTGCACGGCGCACCCGAGTTGAGCCCGCCGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCgccag                          >  5:302898/1‑149 (MQ=255)

                                                                                                                              cTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCgccagc                         <  8:132471/149‑1 (MQ=255)

                                                                                                                              cTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCgccagc                         <  5:118242/149‑1 (MQ=255)

                                                                                                                                 gctgctCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAAcc                                           >  3:404298/1‑128 (MQ=255)

                                                                                                                                 gctgctCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAAcc                                           <  4:404298/128‑1 (MQ=255)

                                                                                                                                  ctgctCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGt                     <  7:359855/149‑1 (MQ=255)

                                                                                                                                  ctgctCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGt                     <  7:326635/149‑1 (MQ=255)

                                                                                                                                       cgcgCCCATGGCCCTGGCCGCCCGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCACCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCgg                >  7:64025/1‑149 (MQ=255)

                                                                                                                                        gcgcCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCg                 <  8:260297/147‑1 (MQ=255)

                                                                                                                                        gcgcCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGc               <  8:264030/149‑1 (MQ=255)

                                                                                                                                        gcgcCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGc               >  1:101487/1‑149 (MQ=255)

                                                                                                                                         tcttcATGGCCAGGGCCGGCGGCCAGGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGa                                        <  3:37885/119‑1 (MQ=38)

                                                                                                                                         tcttcATGGCCAGGGCCGGCGGCCAGGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGa                                        >  4:37885/5‑123 (MQ=38)

                                                                                                                                          gcCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGc               >  1:402239/1‑147 (MQ=255)

                                                                                                                                          gcCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCa             <  1:57589/149‑1 (MQ=255)

                                                                                                                                            ccATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGCCCCGCGCCAGCCAGTGCCGGCCAGc           >  1:250305/1‑149 (MQ=255)

                                                                                                                                               tcgCCCGGTCCGCCGGCCCTGTCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGGCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGAc        <  6:302898/147‑1 (MQ=255)

                                                                                                                                               tGGCCAGGGCCGGCGGCCAGGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCgccagc                         >  5:349934/1‑132 (MQ=255)

                                                                                                                                                 gcgcTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGTTCACCGCCGTGGCGCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACg       <  6:258519/145‑1 (MQ=255)

                                                                                                                                                 gCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACgg      <  5:177882/149‑1 (MQ=255)

                                                                                                                                                     tGGCCGCCGGCCCTGGCCATGAAGACCATGTGAACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGGCGcc  >  1:267740/1‑149 (MQ=255)

AACATTTTGCGCACGAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGGCGCC  >  NC_002947/5502988‑5503284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`