BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,503,149	T→G	5.4%	P33P (CCT→CCG) ‡	oprC →	copper receptor OprC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,503,149	0	T	G	5.4%	337.4 / 9.4	111	P33P (CCT→CCG) ‡	oprC	copper receptor OprC
Reads supporting (aligned to +/- strand): ref base T (55/50); new base G (3/3); total (58/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.62e-01

Only 100 of 111 total aligned reads displayed.

GAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGGCGCCGACTACCTGAAG  >  NC_002947/5503002‑5503296

gAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTg                                                                                                                                                    <  4:318047/149‑1 (MQ=255)

 caGCCAGGAGGCTTCGCGCTAACTCATGACTGCTCGACTGGAATCATTATGTCCGGCGGC‑ACCCCTGTTTGTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCGGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTgg                                                                                                                                                   <  3:170818/148‑1 (MQ=255)

  aGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGc                                                                                                                                                  <  1:158879/149‑1 (MQ=255)

  aGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGc                                                                                                                                                  >  8:384377/1‑149 (MQ=255)

  aGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGc                                                                                                                                                  <  7:7912/149‑1 (MQ=255)

   gCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGcc                                                                                                                                                 <  2:258633/149‑1 (MQ=255)

   gCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGc                                                                                                                                                  >  6:216495/1‑148 (MQ=255)

   gCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGc                                                                                                                                                  >  2:149457/1‑148 (MQ=255)

   gCCAGGAGGCTTCGCGCTAACCCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGcc                                                                                                                                                 <  2:134869/149‑1 (MQ=255)

        gaggCTTCGTGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCACTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATg                                                                                                                                              >  8:359855/1‑147 (MQ=255)

        gaggCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGaa                                                                                                                                            <  7:411576/149‑1 (MQ=255)

        gaggCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGaa                                                                                                                                            <  2:51710/149‑1 (MQ=255)

           gCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCAGGGCCGGCGGCCAGGGCCATggg                                                                                                                                            >  3:21069/1‑144 (MQ=255)

           gCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCAGGGCCGGCGGCCAGGGCCATggg                                                                                                                                            <  4:21069/146‑3 (MQ=255)

                   cTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCa                                                                                                                                 >  5:268318/1‑149 (MQ=255)

                        tCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCacgacg                                                                                                                            >  3:363061/1‑149 (MQ=255)

                          aTGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTCCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCa                                                                                                                          >  8:298210/1‑149 (MQ=255)

                            gACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCAcc                                                                                                                        >  2:57589/1‑149 (MQ=255)

                              cTGATCGACTGGAATCATTATGTCCGGCTGCAACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCAccc                                                                                                                       >  1:265751/1‑149 (MQ=255)

                                 aTCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGt                                                                                                                   >  7:125374/1‑149 (MQ=255)

                                      cTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGc                                                                                                              >  3:73626/1‑149 (MQ=255)

                                         gAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCTTGAAGACCATGTGCACGACGCACCCGAGTTGAGccc                                                                                                            >  4:207057/1‑148 (MQ=255)

                                         gAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGcc                                                                                                             <  2:265751/147‑1 (MQ=255)

                                         gAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCg                                                                                                           >  7:114112/1‑149 (MQ=255)

                                         gAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCCAGTTGAGCCCg                                                                                                           >  3:337278/1‑149 (MQ=255)

                                          aaTCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGAACGACGCACCCGAGTTAAGACCGa                                                                                                          >  8:402379/1‑149 (MQ=255)

                                           aTCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGAc                                                                                                         >  5:258519/1‑149 (MQ=255)

                                            tCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACg                                                                                                        >  8:326635/1‑149 (MQ=255)

                                            tCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACg                                                                                                        >  8:370347/1‑149 (MQ=255)

                                              aTTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGt                                                                                                      <  7:298210/149‑1 (MQ=255)

                                               ttATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTg                                                                                                     <  7:402379/149‑1 (MQ=255)

                                                  tGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGAt                                                                                                   >  4:293353/1‑148 (MQ=255)

                                                    tCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCa                                                                                                 <  3:293353/148‑1 (MQ=255)

                                                         cTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCAccgccg                                                                                           >  7:86476/1‑149 (MQ=255)

                                                          tGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGCCCGCCGGCCCTGGCCAGGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGt                                                                                          >  5:154614/1‑149 (MQ=255)

                                                                cccTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGccc                                                                                     >  8:150497/1‑149 (MQ=255)

                                                                 ccTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGcccc                                                                                    <  7:336315/149‑1 (MQ=255)

                                                                 ccTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGcccc                                                                                    <  8:125374/149‑1 (MQ=255)

                                                                 ccTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGcccc                                                                                    <  4:363061/149‑1 (MQ=255)

                                                                  cTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGcccc                                                                                    >  7:264030/1‑148 (MQ=255)

                                                                  cTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGcccc                                                                                    >  5:90502/1‑148 (MQ=255)

                                                                   tGTTTTTGCCTTGTCCGTGCGTGGGACCATGGCCGCCGTGTGGTGCTCGCTGCTCGGGCCCATGGCACTGGCCGCCGTCCCTGGCCATGAAGACCATGTTCACGACGCTCCCGAGTTTAGCCCGACGGTGATCACCGCCGTGGCCCCGa                                                                                  <  4:337278/149‑1 (MQ=255)

                                                                     tttttGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGt                                                                                >  1:355125/1‑149 (MQ=255)

                                                                     tttttGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGt                                                                                >  7:260297/1‑149 (MQ=255)

                                                                           ccTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCCCCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTcgccg                                                                          >  5:37030/1‑149 (MQ=255)

                                                                           ccTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGCCCCCGAGTTcgccg                                                                          <  7:370347/149‑1 (MQ=255)

                                                                            cTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTcgccgc                                                                         >  8:212015/1‑149 (MQ=255)

                                                                            cTTGTCCCTGCGTGGGACCAGCGCCGCCCTGTTCGGATCGATTCTCCCGCCCATGGCCCTGGCCGCCGGCCCTGTCCATGAAGACCATGTGAACGACGCACCCGAGTTGAGCCCTACGGTGATCACCGCCGTGGCCCCGAGTTcgccgc                                                                         <  6:304060/149‑1 (MQ=255)

                                                                                  ccTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACtg                                                                   >  6:322834/1‑149 (MQ=255)

                                                                                   cTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACtgt                                                                  >  1:402979/1‑149 (MQ=255)

                                                                                    tGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTcgc                                                                            <  5:184072/138‑1 (MQ=255)

                                                                                    tGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTcgc                                                                            >  6:184072/1‑138 (MQ=255)

                                                                                    tGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACtgtg                                                                 >  8:212353/1‑149 (MQ=255)

                                                                                      cGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACtgtg                                                                 <  6:90502/147‑1 (MQ=255)

                                                                                            aCCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCaa                                                         >  3:283361/1‑149 (MQ=255)

                                                                                             ccATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAAc                                                        >  7:408816/1‑149 (MQ=255)

                                                                                              cATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAAcc                                                       >  6:118242/1‑149 (MQ=255)

                                                                                               aTCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAAccc                                                      <  7:212015/149‑1 (MQ=255)

                                                                                                 cgccgcCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCg                                                     >  7:38518/1‑148 (MQ=255)

                                                                                                  gccgccCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGaa                                                   <  8:38518/149‑1 (MQ=255)

                                                                                                  gccgccCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGa                                                    <  3:422987/148‑1 (MQ=255)

                                                                                                  gccgccCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGa                                                    <  5:322834/148‑1 (MQ=255)

                                                                                                  gccgccCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGCGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGa                                                    <  1:149457/148‑1 (MQ=255)

                                                                                                   ccgccCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGaaa                                                  >  8:276677/1‑149 (MQ=255)

                                                                                                    cgccCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAg                                                 <  5:130251/149‑1 (MQ=255)

                                                                                                       ccTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGAcc                                              <  7:406808/149‑1 (MQ=255)

                                                                                                          gtgCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCcgc                                           <  2:402979/149‑1 (MQ=255)

                                                                                                           tgCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCcgcg                                          <  7:276677/149‑1 (MQ=255)

                                                                                                              ggCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCgcca                                       <  5:39476/149‑1 (MQ=255)

                                                                                                               gCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCCTGAAGACCATGTGCACGGCGCACCCGAGTTGAGCCCGCCGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCgccag                                      >  5:302898/1‑149 (MQ=255)

                                                                                                               gCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCgccag                                      >  8:16269/1‑149 (MQ=255)

                                                                                                                cTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCgccagc                                     <  8:132471/149‑1 (MQ=255)

                                                                                                                cTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCgccagc                                     <  5:118242/149‑1 (MQ=255)

                                                                                                                   gctgctCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAAcc                                                       <  4:404298/128‑1 (MQ=255)

                                                                                                                   gctgctCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAAcc                                                       >  3:404298/1‑128 (MQ=255)

                                                                                                                    ctgctCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGt                                 <  7:326635/149‑1 (MQ=255)

                                                                                                                         cgcgCCCATGGCCCTGGCCGCCCGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCACCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCgg                            >  7:64025/1‑149 (MQ=255)

                                                                                                                          gcgcCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGc                           <  8:264030/149‑1 (MQ=255)

                                                                                                                          gcgcCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGc                           >  1:101487/1‑149 (MQ=255)

                                                                                                                           tcttcATGGCCAGGGCCGGCGGCCAGGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGa                                                    <  3:37885/119‑1 (MQ=38)

                                                                                                                           tcttcATGGCCAGGGCCGGCGGCCAGGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGa                                                    >  4:37885/5‑123 (MQ=38)

                                                                                                                            gcCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGc                           >  1:402239/1‑147 (MQ=255)

                                                                                                                            gcCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCa                         <  1:57589/149‑1 (MQ=255)

                                                                                                                              ccATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGCCCCGCGCCAGCCAGTGCCGGCCAGc                       >  1:250305/1‑149 (MQ=255)

                                                                                                                                 tcgCCCGGTCCGCCGGCCCTGTCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGGCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGAc                    <  6:302898/147‑1 (MQ=255)

                                                                                                                                 tGGCCAGGGCCGGCGGCCAGGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCgccagc                                     <  6:349934/132‑1 (MQ=255)

                                                                                                                                 tGGCCAGGGCCGGCGGCCAGGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCgccagc                                     >  5:349934/1‑132 (MQ=255)

                                                                                                                                   gcgcTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGTTCACCGCCGTGGCGCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACg                   <  6:258519/145‑1 (MQ=255)

                                                                                                                                   gCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCTGCCAGCGACgg                  >  2:109842/1‑149 (MQ=255)

                                                                                                                                   gCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACgg                  <  5:177882/149‑1 (MQ=255)

                                                                                                                                       tGGCCGCCGGCCCTGGCCATGAAGACCATGTGAACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGGCGcc              >  1:267740/1‑149 (MQ=255)

                                                                                                                                        ggCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGCCGGCGcc              >  4:217603/1‑148 (MQ=255)

                                                                                                                                         gccgccGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGGcgacga            >  8:116003/1‑149 (MQ=255)

                                                                                                                                         gccgccGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGGCGCCGa            <  7:150497/149‑1 (MQ=255)

                                                                                                                                           cgccgGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGGCGCCGAc           >  6:50604/1‑148 (MQ=255)

                                                                                                                                           cgccgGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGGCGCCGACt          <  7:16269/149‑1 (MQ=255)

                                                                                                                                            gccgGCCCTGGCCATGAAGACCATGTACACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGGCGCCGACTa         >  6:183010/1‑149 (MQ=255)

                                                                                                                                                gCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGGCGCCGACTACCTg     <  4:83448/149‑1 (MQ=255)

                                                                                                                                                  ccTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGGCGCCGACTACCTg     >  8:231224/1‑147 (MQ=255)

                                                                                                                                                   cTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGGCGCCGACTACCTGAAg  >  8:223472/1‑149 (MQ=255)

GAAGCCAGGAGGCTTCGCGCTAACTCATGACTGATCGACTGGAATCATTATGTCCGGCTGC‑ACCCCTGTTTTTGCCTTGTCCCTGCGTGGGACCATCGCCGCCCTGTGCGGCTCGCTGCTCGCGCCCATGGCCCTGGCCGCCGGCCCTGGCCATGAAGACCATGTGCACGACGCACCCGAGTTGAGCCCGACGGTGATCACCGCCGTGGCCCCGAGTTCGCCGCTGACTGTGGTCACCAACCCGAAAGACCCGCGCCAGCCAGTGCCGGCCAGCGACGGCGCCGACTACCTGAAG  >  NC_002947/5503002‑5503296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`