| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,535,425 | G→C | 5.5% | E148Q (GAG→CAG) | pncB → | nicotinate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,535,425 | 0 | G | C | 5.5% | 281.1 / 4.3 | 87 | E148Q (GAG→CAG) | pncB | nicotinate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (39/43); new base C (2/3); total (41/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01 | |||||||||||
CGTTACGTGCACGTCGGCATCGAAAATGATCAGCTGTTCCTGCGCCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCGGCACCCGCCGGCGCTTTTCCAGCCGCGTGCAGGAGGAAGTGGCAAGGG > NC_002947/5535278‑5535563 | cGTTACGTGCACGTCGGCATCGAAAATGATCAGCTGTTCCTGCGCCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGa < 7:51614/149‑1 (MQ=255) gTGCACGTCGGCATCGAAAATGATCAGCTGTTCCTGCGCCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCTCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTCGGCCAGgcgca < 6:535/149‑2 (MQ=255) gTGCACGTCGGCATCGAAAATGATCAGCTGTTCCTGCGCCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGcgcg > 5:157297/1‑149 (MQ=255) tGCACGTCGGCTTCGAACATGATCAGCTGATCCTGCGCCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCGGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCgg < 6:225953/149‑1 (MQ=255) gTCGGCATCGAAAATGATCAGCTGTTCCTGCGCCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGAc > 6:240486/1‑147 (MQ=255) gTCGGCATCGAAAATGATCAGCTGTTCCTGCGCCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCa < 1:406191/149‑1 (MQ=255) tCGGCATCGAAAATGATCAGCTGTTCCTGCGCCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAg < 6:242841/149‑1 (MQ=255) tCGGCATCGAAAATGATCAGCTGTTCCTGCGCCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAg < 3:358990/149‑1 (MQ=255) tCGGCATCGAAAATGATCAGCTGTTCCTGCGCCTGAAAGGTCCGTGACTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAg < 2:2209/149‑1 (MQ=255) cGGCATCGAAAATGATCAGCTGTTCCTGCGCCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATAATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGt > 2:361740/1‑149 (MQ=255) gCATCGAAAATGATCAGCTGTTCCTGCGCCTGAAAGGTCCGTGGCTGCCTGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACCTGCGCCTGGCCGAGGCGCGGGACCAGTTg < 3:281182/149‑1 (MQ=255) aTCGAAAATGATCAGCTGTTCCTGCGCCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCTCATGCGCCTGGCCGAGGCGCGGGACCAGTTGTa < 1:52928/149‑1 (MQ=255) aTCGAAAATGATCAGCTGTTCCTGCGCCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTa > 4:35600/1‑149 (MQ=255) aTCGAAAATAATCAGCTGTTCCTGCGCCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTa < 7:147135/149‑1 (MQ=255) gAAAATGATCAGCTGTTCCTGCGCCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCg > 2:232117/1‑149 (MQ=255) aaaaTGATCAGCTGTTCCTGCGCCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCg > 1:196922/1‑148 (MQ=255) aaaaTGATCAGCTGTTCCTGCGCCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCg > 2:208707/1‑148 (MQ=255) aaTGATCAGCTGTTCCTGCGCCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCaa < 3:35600/149‑1 (MQ=255) cAGCTGTTCCTGCGCCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGa > 3:57638/1‑149 (MQ=255) aGCTGTTCCTGCGCCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGAc < 2:72554/149‑1 (MQ=255) gCTGTTCCTGCGCCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACt > 5:293079/1‑149 (MQ=255) cTGTTCCTGCGCCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTg > 8:287767/1‑149 (MQ=255) ttCCTGCGCCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTgg < 5:262598/147‑1 (MQ=255) ttCCTGCGCCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTgg < 6:7101/147‑1 (MQ=255) ttCCTGCGCCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGc < 1:208707/148‑1 (MQ=255) cTGCGCCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCtgc < 2:156121/148‑1 (MQ=255) gcCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCtgcgtgcg > 8:109113/1‑149 (MQ=255) gcCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCtgc < 8:323397/144‑1 (MQ=255) gcCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCtgc > 7:323397/1‑144 (MQ=255) ccTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTCGGCCAGgcgcat < 3:373369/112‑3 (MQ=255) ccTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTCGGCCAGgcgcat > 4:373369/1‑110 (MQ=255) cTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCa > 6:55513/1‑149 (MQ=255) cTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGAGTGCGCa > 6:61845/1‑149 (MQ=255) aGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCCCATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGc > 4:39584/1‑147 (MQ=255) aGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCa < 5:348011/149‑1 (MQ=255) aGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCa < 5:240486/149‑1 (MQ=255) tcggTGGCTGGATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATGATCTGCG‑AAGTACGCAAGCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCTGGACCATTTGTACCGCATGTTCGACTGGCTGCGTGCGCATGCCAGcg < 6:55106/146‑1 (MQ=255) cGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGcgac > 6:418806/1‑149 (MQ=255) tggcgCATGTGCTTCCTTTTCGATGTGCCGCTGC‑GGGCATCATTATCGCATGTA‑GCCAACGCCATCTTGCCCCCCCAATTGGGCCTGGCGCAGGGGGGGTACCTG‑TGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGcgacgac < 4:19125/145‑1 (MQ=255) tGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAg > 7:61732/1‑149 (MQ=255) cATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAg < 7:109113/147‑1 (MQ=255) cTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAAc < 7:147520/148‑1 (MQ=255) cGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAg < 8:267960/148‑1 (MQ=255) gAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTgg > 4:93569/1‑105 (MQ=255) gAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTgg < 3:93569/105‑1 (MQ=255) gAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGt > 1:412755/1‑149 (MQ=255) aGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTgg < 8:61732/149‑1 (MQ=255) gCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGcc < 4:57638/148‑1 (MQ=255) ccGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTgg < 7:343338/145‑1 (MQ=255) ccGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTgg > 8:343338/1‑145 (MQ=255) gctgctGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGCCt > 4:360582/1‑149 (MQ=255) tgctgGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGt > 4:250893/1‑139 (MQ=255) tgctgGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGt < 3:250893/139‑1 (MQ=255) ctgGCCATCATCAGCG‑AAGTACTCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCg < 5:61845/148‑1 (MQ=255) ctgGCCATCATCAGCG‑AAGTACGCTACCGCAATG‑TGCACCCGCACATGCGCCTGGCCGAGGGGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGGGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTAGCCGACTTCg < 1:361740/148‑1 (MQ=255) ctgGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCg < 5:55513/148‑1 (MQ=255) ctgGCAATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCgg < 2:196922/149‑1 (MQ=255) ggCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCGGCa > 5:221678/1‑149 (MQ=255) ggCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCGGCa > 5:104208/1‑149 (MQ=255) ggCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCGGCa > 3:232968/1‑149 (MQ=255) catcatCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCGGCAc > 1:17265/1‑147 (MQ=255) catcatCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCGGCAc < 2:17265/147‑1 (MQ=255) caGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCGGCACCCGCCGgc > 8:232055/1‑149 (MQ=255) g‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCGGCACCCGCCGGCGCtt < 2:377697/149‑1 (MQ=255) g‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCGGCACCCGCCGGCGCtt > 3:11046/1‑149 (MQ=255) cGCAACCGAAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCGGCACCCGCCGGCGCTTTTCCa < 1:232117/148‑1 (MQ=255) gCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTCCGGCACCCGCCGGCGCTTTTCCAGc > 5:190313/1‑149 (MQ=255) gCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACCTCGGCACCCGCCGGCGCTTTTCCAGc > 5:54435/1‑149 (MQ=255) aaCCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCGGCACCCGCCGGCGCTTTTCCAGCcg > 3:394870/1‑149 (MQ=255) aCCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCGGCACCCGCCGGCGCTTTTCCAGCcgc < 1:263540/149‑1 (MQ=255) gCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCGGCACCCGCCGGCGCTTTTCCAGCCGCGTg < 7:297612/149‑1 (MQ=255) aaTC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCGGCACCCGCCGGCGCTTTTCCAGCCGCGTg > 4:185400/1‑147 (MQ=255) aaTC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCGGCACCCGCCGGCGCTTTTCCAGCCGCGTGCa < 5:418806/149‑1 (MQ=255) aTC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCGGCACCCGCCGGCGCTTTTCCAGCCGCGTGCag < 6:293079/149‑1 (MQ=255) tGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGAATGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCGGCACCCGCCGGCGCTTTTCCAGCCGCGTGCaggag > 2:304852/1‑149 (MQ=255) cACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACCTCGGCACCCGCCGGCGCTTTTCCAGCCGCGTGCaggagg < 6:54435/148‑1 (MQ=255) aCCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCGGCACCCGCCGGCGCTTTTCCAGCCGCGTGCaggagg > 5:56547/1‑147 (MQ=255) aCCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCGGCACCCGCCGGCGCTTTTCCAGCCGCGTGCaggagg > 3:12889/1‑147 (MQ=255) ccGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCGGCACCCGCCGGCGCtt > 7:261262/1‑125 (MQ=255) ccGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCGGCACCCGCCGGCGCtt < 8:261262/125‑1 (MQ=255) ccGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCGGCACCCGCCGGCGCTTTTCCAGCCGCGTGCAGGAGGAAGt > 7:39246/1‑149 (MQ=255) ccGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCGGCACCCGCCGGCGCTTTTCCAGCCGCGTGCAGGAGGAAGt > 7:226026/1‑149 (MQ=255) tggtcccgcgcCTCGGCCAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCGGCACCCGCCGGCGCTTTTCCAGCCGCGTGCAGGAGGAAGTg > 3:419767/8‑149 (MQ=255) cacaTGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCGGCACCCGCCGGCGCTTTTCCAGCCGCGTGCAGGAGGAAGTGGc < 3:39584/149‑1 (MQ=255) cacaTGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTCCGGCACCCGCCGGCGCTTTTCCAGCCGCGTGCAGGAGGAAGTGGc < 6:190313/149‑1 (MQ=255) tGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCGGCACCCGCCGGCGCTTTTCCAGCCGCGTGCAGGAGGAAGTGGCAAgg < 7:30327/149‑1 (MQ=255) gcgcCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCGGCACCCGCCGGCGCTTTTCCAGCCGCGTGCAGGAGGAAGTGGCAAggg > 4:165241/1‑149 (MQ=255) | CGTTACGTGCACGTCGGCATCGAAAATGATCAGCTGTTCCTGCGCCTGAAAGGTCCGTGGCTGCATGTGA‑TCCTGTTCGAAGTGCCGCTGCTGGCCATCATCAGCG‑AAGTACGCAACCGCAATC‑TGCACCCGCACATGCGCCTGGCCGAGGCGCGGGACCAGTTGTACCGCAAGTTCGACTGGCTGCGTGCGCATGCCAGCGACGACGAGCTGGCCGAACTGCAGGTGGCCGACTTCGGCACCCGCCGGCGCTTTTCCAGCCGCGTGCAGGAGGAAGTGGCAAGGG > NC_002947/5535278‑5535563 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |