Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,548,700 C→A 5.6% Q118H (CAG→CAT)  rnr ← exoribonuclease R

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,548,7000CA 5.6% 322.9 / 5.6 99Q118H (CAG→CAT) rnrexoribonuclease R
Reads supporting (aligned to +/- strand):  ref base C (39/54);  new base A (3/3);  total (42/57)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCCGATGCCGCCTTCTTCGAAATAGCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCGGGGCATAAGTGCCGCGCCGGGTATAGATAAGCTGGC  >  NC_002947/5548552‑5548845
                                                                                                                                                     |                                                                                                                                                  
aGCCGATGCCGCCTTCTTCGAAATAGCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATc                                                                                                                                                    >  4:259504/1‑149 (MQ=255)
         cgccTTCTTCGAAATAGCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCACTACCATCACGGCGGCCCCGGCGGTGGCCACCGGAGACCGCGGCCAAGCCCGGGTCGCCGGAGAACACCCGTAGCATATGcggcgg                                                                                                                                            >  1:131472/1‑149 (MQ=255)
         cgccTTCTTCGAAATAGCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACggg                                                                                                                                           <  4:47683/149‑1 (MQ=255)
         cgccTTCTTCGAAATAGCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCAGATGCGACtgg                                                                                                                                           <  2:225703/149‑1 (MQ=255)
          gccTTCTTCGAAATAGCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCAGATGCGACtggt                                                                                                                                          <  4:134249/149‑2 (MQ=255)
            cttcttCGAAATAGCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGCTCGCCGACGAACACCAGTCGCATCTGCGACGGGCt                                                                                                                                         >  6:139943/1‑148 (MQ=255)
               cttcGTAATAGCGTCCGACCACGCTTTCGTTGGCACGGGAGATGACTTCGACCAGCAG‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCTCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGa                                                                                                                                       <  6:158300/147‑1 (MQ=255)
               cttcGAAATAGCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGa                                                                                                                                       <  4:405352/147‑1 (MQ=255)
                ttcGAAATAGCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGTCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAgg                                                                                                                                     >  7:57901/1‑148 (MQ=255)
                ttcGAAATAGCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACg                                                                                                                                             <  1:175412/140‑1 (MQ=255)
                ttcGAAATAGCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTg                                                                                                                                        <  5:443037/145‑1 (MQ=255)
                ttcGAAATAGCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTg                                                                                                                                        >  6:443037/1‑145 (MQ=255)
                ttcGAAATAGCGGCCGACCACGCATTCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAgg                                                                                                                                     >  8:68028/1‑148 (MQ=255)
                    aaaTAGCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAg                                                                                                                                <  3:315406/149‑1 (MQ=255)
                    aaaTAGCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAg                                                                                                                                <  3:338297/149‑1 (MQ=255)
                         gCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCtt                                                                                                                           <  6:94954/149‑1 (MQ=255)
                               gACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGc                                                                                                                     >  5:120961/1‑149 (MQ=255)
                               gACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGc                                                                                                                     <  4:288564/149‑1 (MQ=255)
                                aCCACGCTTTCGTGGGCATGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGcc                                                                                                                    <  6:49147/149‑1 (MQ=255)
                                 ccACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCg                                                                                                                   <  3:259504/149‑1 (MQ=255)
                                 ccACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACAAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCg                                                                                                                   >  1:362532/1‑149 (MQ=255)
                                  cACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGt                                                                                                                  >  2:376731/1‑149 (MQ=255)
                                   aCGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGt                                                                                                                  >  4:318329/1‑148 (MQ=255)
                                    cGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCa                                                                                                                >  7:165178/1‑149 (MQ=255)
                                     gCTTTCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCAt                                                                                                               >  8:348143/1‑149 (MQ=255)
                                       tttCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCg                                                                                                             <  6:120961/149‑1 (MQ=255)
                                       tttCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCAGATGCGACtggt                                                                                                                                          <  3:102590/120‑2 (MQ=255)
                                       tttCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCAGATGCGACtggt                                                                                                                                          >  4:102590/1‑119 (MQ=255)
                                         tCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCgg                                                                                                            <  5:15630/148‑1 (MQ=255)
                                         tCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCg                                                                                                             <  7:348143/147‑1 (MQ=255)
                                         tCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCg                                                                                                             <  8:293418/147‑1 (MQ=255)
                                           gTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGATGCCGTCATCGGGaa                                                                                                         >  3:346546/1‑149 (MQ=255)
                                            tGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGaa                                                                                                         >  7:121707/1‑148 (MQ=255)
                                              ggCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATaa                                                                                                      <  5:217379/149‑1 (MQ=255)
                                              ggCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATa                                                                                                       <  5:102918/148‑1 (MQ=255)
                                                cACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGTCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAgg                                                                                                    <  8:57901/149‑1 (MQ=255)
                                                 aCGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAgg                                                                                                    >  3:171074/1‑148 (MQ=255)
                                                   gggAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGgaag                                                                                                 >  1:30102/1‑149 (MQ=255)
                                                   gggAGATGACTTAGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGgaag                                                                                                 >  4:39831/1‑149 (MQ=255)
                                                     gagaTGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGgaagcc                                                                                               >  2:186464/1‑149 (MQ=255)
                                                      agaTGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGgaagcc                                                                                               >  4:18885/1‑148 (MQ=255)
                                                         tGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGgaagccgaag                                                                                           >  8:170874/1‑149 (MQ=255)
                                                             ttCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGt                                                                                       >  5:152705/1‑149 (MQ=255)
                                                                 aCCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTcgcg                                                                                   >  2:255543/1‑149 (MQ=255)
                                                                  ccAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTACCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCgg                                                                                  >  7:266894/1‑149 (MQ=255)
                                                                   cAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCTGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAATGAAGCCGAAGCCGTCGCGG‑t                                                                                <  3:39831/149‑1 (MQ=255)
                                                                   cAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑t                                                                                >  4:187334/1‑149 (MQ=255)
                                                                   cAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑t                                                                                >  3:333387/1‑149 (MQ=255)
                                                                    aGCAC‑GCCTTCACGGTGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑Tg                                                                               <  3:74983/149‑1 (MQ=255)
                                                                      cAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGc                                                                             <  6:152705/149‑1 (MQ=255)
                                                                        c‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCg                                                                           >  8:353785/1‑149 (MQ=255)
                                                                        c‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCg                                                                           <  4:65234/149‑1 (MQ=255)
                                                                            cTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGgag                                                                        >  3:130414/1‑149 (MQ=255)
                                                                               cACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGgaga                                                                       <  2:30102/147‑1 (MQ=255)
                                                                               cACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGAc                                                                      <  3:296249/148‑1 (MQ=255)
                                                                               cACGGCGGCCACGGCGGGCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCTCATCTGCTACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGgaga                                                                       <  2:184774/147‑1 (MQ=255)
                                                                                aCGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGAcgc                                                                    <  1:1746/149‑1 (MQ=255)
                                                                                  ggcggcCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGa                                                                  <  1:255543/149‑1 (MQ=255)
                                                                                   gcggcCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGAc                                                                 <  5:36106/149‑1 (MQ=255)
                                                                                   gcggcCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGAc                                                                 <  8:165178/149‑1 (MQ=255)
                                                                                   gcggcCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGAc                                                                 <  3:26313/149‑1 (MQ=255)
                                                                                    cggcCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGAcc                                                                >  5:136704/1‑149 (MQ=255)
                                                                                    cggcCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGAc                                                                 >  2:145663/1‑148 (MQ=255)
                                                                                       ccACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGgaa                                                                                                  >  8:57/1‑113 (MQ=255)
                                                                                       ccACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGgaa                                                                                                  <  7:57/113‑1 (MQ=255)
                                                                                         aCGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACca                                                               >  8:60868/1‑145 (MQ=255)
                                                                                          cggcggTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGAt                                                          <  7:60868/149‑1 (MQ=255)
                                                                                           ggcggTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATc                                                         <  3:187334/149‑1 (MQ=255)
                                                                                            gcggTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCa                                                        <  1:376731/149‑1 (MQ=255)
                                                                                             cggTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCAg                                                       <  1:299918/149‑1 (MQ=255)
                                                                                                 cGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAgg                                                                                                                                     <  5:304271/68‑1 (MQ=255)
                                                                                                 cGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAgg                                                                                                                                     >  6:304271/1‑68 (MQ=255)
                                                                                                 cGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCAGGTcc                                                   <  8:80075/149‑1 (MQ=255)
                                                                                                  gACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCAGGTCCa                                                  <  1:432333/149‑1 (MQ=255)
                                                                                                   acacCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTACCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCAGGTCCAg                                                 <  8:266894/149‑1 (MQ=255)
                                                                                                    cacCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCAGGTCCAGc                                                <  3:332910/149‑1 (MQ=255)
                                                                                                     acCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCAGGTCCAGCt                                               >  4:335844/1‑149 (MQ=255)
                                                                                                       cGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTg                                             <  5:346634/149‑1 (MQ=255)
                                                                                                            aCGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTAc                                        >  5:24155/1‑149 (MQ=255)
                                                                                                                gggCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCggg                                    >  4:419515/1‑149 (MQ=255)
                                                                                                                gggCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCggg                                    <  2:362532/149‑1 (MQ=255)
                                                                                                                gggCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCggg                                    >  2:354640/1‑149 (MQ=255)
                                                                                                                gggCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCggg                                    <  3:419515/149‑1 (MQ=255)
                                                                                                                      aGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCGGGGCATaa                              <  4:130414/149‑1 (MQ=255)
                                                                                                                       gCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCGGGGCATAAg                             >  7:278217/1‑149 (MQ=255)
                                                                                                                        ccGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCGGGGCATAAGt                            <  4:171074/149‑1 (MQ=255)
                                                                                                                          gcgGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCGGGGCATAAGTGc                          <  7:68028/149‑1 (MQ=255)
                                                                                                                          gcgGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCGGGGCATAAGTGc                          <  6:274433/149‑1 (MQ=255)
                                                                                                                                  cGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCGGGGCATAAGTGCCGCGCCgg                  <  3:423742/149‑1 (MQ=255)
                                                                                                                                      gAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCGGGGCATAAGTGCCGCGCCGGGtat              <  2:21517/149‑1 (MQ=255)
                                                                                                                                        acacCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGCCGCGACCACAGATCAGGTCCAGCTTGTCTACCGGGGCATAAGTGCCGCGCCgggga               >  8:380606/1‑144 (MQ=255)
                                                                                                                                        acacCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCGGGGCATAAGTGCCGCGCCGGGTATAg            <  6:24155/149‑1 (MQ=255)
                                                                                                                                         cacCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCGGGGCATAAGTGCCGCGCCGGGTATAGa           <  7:170874/149‑1 (MQ=255)
                                                                                                                                          acCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCGGGGCATAAGTGCCGCGCCGGGTATAGAt          <  4:333387/149‑1 (MQ=255)
                                                                                                                                           ccAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCGGGGCATAAGTGCCGCGCCGGGTATAGAt          >  3:426307/1‑148 (MQ=255)
                                                                                                                                           ccAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAACGAAGCCGAAGCCGTCGCGGCTTGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCGGGGCATAAGTGCCGCGCCGGGTATAGAt          <  1:354640/149‑1 (MQ=255)
                                                                                                                                            ccgTCGCAGATGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCGGGGCATAAGTGCCGCGCCGGGTATAGAt          >  1:204803/3‑147 (MQ=255)
                                                                                                                                              gTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCGGGGCATAAGTGCCGCGCCGGGTATAGATAAGc      >  5:14689/1‑149 (MQ=255)
                                                                                                                                                 gCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGACGCGG‑TGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCGGGGCATAAGTGCCGCGCCGGGTATAGATAAGCTgg   <  8:121707/149‑1 (MQ=255)
                                                                                                                                                  cATCTGCGACGGGCTGAGGAACGGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCGGGGCATAAGTGCCGCGCCGGGTATAGATAAGCTGGc  >  7:273369/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AGCCGATGCCGCCTTCTTCGAAATAGCGGCCGACCACGCTTTCGTGGGCACGGGAGATGACTTCGACCAGCAC‑GCCTTCACGGCGGCCACGGCGGTCGACACCGGAGACGCGGGCCAAGCCGCGGTCGCCGTCGAACACCAGTCGCATCTGCGACGGGCTGAGGAACAGGTCTTCGCTGCCGTCATCGGGAATAAGGAAGCCGAAGCCGTCGCGG‑TGGCCGGAGACGCGACCACAGATCAGGTCCAGCTTGTCTACCGGGGCATAAGTGCCGCGCCGGGTATAGATAAGCTGGC  >  NC_002947/5548552‑5548845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑