| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,552,913 | T→G | 10.2% | intergenic (+576/+85) | PP_4882 → / ← PP_5717 | iron ABC transporter permease/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,552,913 | 0 | T | G | 10.2% | 126.9 / 6.1 | 55 | intergenic (+576/+85) | PP_4882/PP_5717 | iron ABC transporter permease/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (23/26); new base G (3/3); total (26/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01 | |||||||||||
GTACTCATTTTCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGTGCAATCAGCAAAATTTGT > NC_002947/5552768‑5553058 | gTACTCATTTTCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCg < 7:173742/149‑1 (MQ=255) tCATTTTCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCaa > 5:169622/1‑148 (MQ=255) tCATTTTCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTCCTTAGCaa > 4:233419/1‑148 (MQ=255) cATTTTCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAg > 5:374813/1‑149 (MQ=255) aTTTTCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑c > 8:98315/1‑149 (MQ=255) aTTTTCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGATTCGCAAAG‑c > 6:214457/1‑149 (MQ=255) ttttCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCGAAGCAAAG‑cc < 3:207986/149‑1 (MQ=255) ttCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCGAAGCAAAG‑ccc < 4:69885/148‑1 (MQ=255) cgcgCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGGTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCGAAGCAAAG‑CCCgg < 4:237585/148‑1 (MQ=255) gctctctCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGGACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGa < 6:251795/145‑1 (MQ=255) tcATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTg < 6:374813/147‑1 (MQ=255) aTAAGTCCCTGCTCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGATGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTggg < 7:131670/147‑1 (MQ=255) aTAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGt > 8:278557/1‑149 (MQ=255) aGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGttt > 7:210104/1‑148 (MQ=255) gTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCa < 6:169622/149‑1 (MQ=255) gTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCa < 7:39362/149‑1 (MQ=255) aCTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCGTAGCAAAG‑CCCGGCAACAGGGGTTTGAGCt > 4:289173/1‑149 (MQ=17) tGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGGGTTTGTTAGCCAAGCCCCGGAAAAGGGGGttaaccggg > 2:224119/1‑142 (MQ=17) gcATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCtggtgg < 7:278557/149‑1 (MQ=255) cGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGtt < 8:210104/149‑1 (MQ=255) gTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGttt > 5:140075/1‑149 (MQ=255) tGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAGACTGGGCTTTCAGCTGGTGGCTGCTTGAGATTTTAg > 1:374080/1‑148 (MQ=255) tACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACAGGGGTTTAAGCTGGGGGCTGGTTGAGTGTTTATAAAAc > 4:273742/1‑148 (MQ=17) aCAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACaa > 8:224657/1‑149 (MQ=255) ggTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGtt < 3:256403/149‑1 (MQ=255) ggTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGtt < 3:273742/149‑1 (MQ=255) ccAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCtggt < 5:261030/112‑1 (MQ=255) cAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGGTTCGCAAAT‑CCCGGCAACAGGGGTTTCAGCGGGTGGCTGCGTGAGTTTTTAGAAAACAATGGGGTggcttat > 1:411446/1‑143 (MQ=17) cAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAgtg < 3:49692/124‑1 (MQ=255) cAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCACAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCt > 3:358261/1‑149 (MQ=255) agaagCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCTAACCTGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGt < 3:233419/149‑1 (MQ=255) agaagCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGt < 5:83082/149‑1 (MQ=255) ttATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTTGTTGGCAAAG‑CCCCGGAACTGGGGTTTAACCTGGTGGCTGCGTGAGTGTTTTTAAAACAATTGGTTTCATTATGTTTTACCAggg > 4:187668/1‑149 (MQ=17) ttATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTTGTTAGCAAAG‑CCCGGAAAATGGGGTTTAAGCTGGTGGGGGGGTTTGTGTTTTGAAAACAAATGGTTGCATTATTGTTGACCAggg > 7:150312/1‑149 (MQ=17) gCTGAACAAGGTCTTGAGGAATTCTGAACCAGAAGCCAAGACCGGCAGGTCCCGGCTTTGCGTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCAGTCTGTTTGACCAGGGCTTa > 8:135351/1‑149 (MQ=255) cTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTAc < 6:140075/149‑1 (MQ=255) cTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTAc < 7:98315/149‑1 (MQ=255) cTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTAc < 7:224657/149‑1 (MQ=255) aaGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGa < 5:397985/132‑1 (MQ=255) aaGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGa > 6:397985/1‑132 (MQ=255) ggTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCGGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGt > 7:366066/1‑148 (MQ=255) tCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTAct < 2:42371/149‑1 (MQ=255) gAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTTCTTGGCAAAG‑CCCGGAAACTGGGGTTTAAGGAGGTGGCAGGTTGAGTGTTTATAAAAAAATGGGGTGCATTCAGTTTGACCAGGGCTTACCAGAAGTAATATATcc > 4:141152/1‑148 (MQ=18) tCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGTAACTGGGGTTTAAGCTGGTGGCTGCTTTAGTGTTTATAAAACAATGGGTTGGATTGTGTTTGGCCAGGGCTTACCGAAAGTACTCTATACAAAg > 3:98071/1‑148 (MQ=18) ccGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCACAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGa < 4:358261/148‑1 (MQ=255) gAAGCCAAGACCGGCAGGTCCGGGCTTTTCTTAGCAAAA‑CCCCGAAAAAGGGGTTTAAGCTTGTGGGAGGGTGTATGTTTTGAAAAAAATGTGTTGCATTCTGTTTTGACAGGGCTTAACACAAGTAATGGAAGCaaaaaaaaaaaat > 4:393536/1‑147 (MQ=9) gCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACa < 3:167240/149‑1 (MQ=255) ccGTCAGGTCCGTGCTTTGCTTCGAAAAG‑CCCGGAAACTTGGGTTTCAGCTGGTTGCTGCTTGAGTGTTTATAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGt < 3:408593/148‑1 (MQ=255) gggCTTTGCGAAGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGTGCAATCAGCaa > 7:184152/1‑148 (MQ=255) gCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGTGCAATCAGAAAAAt > 3:170679/1‑149 (MQ=255) tttGCTTCTCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGTGCAATCAGCAAAAttt < 3:78240/149‑1 (MQ=255) tGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGTGCAATCAGCAAAAttt < 4:98071/147‑1 (MQ=255) tGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGTGCAATCAGCAAAAttt < 2:411446/147‑1 (MQ=255) tGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGTGCAATCAGCAAAATTTg > 3:429896/1‑148 (MQ=255) gCTTCGCAAAG‑CCCGGAAACTTGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGTGCAATCAGCAAAATTTGt < 5:214457/148‑1 (MQ=255) | GTACTCATTTTCGCGCTCTCATAAGTCACTGCGCATCAGTTGGTACGTCTGGTGTACAGCCGGTTCACCAAGAAGCAGGCTTATGCTGAACAAGGTCTTGAGGAATTCCGAACCAGAAGCCAAGACCGGCAGGTCCGGGCTTTGCTTCGCAAAG‑CCCGGAAACTGGGGTTTCAGCTGGTGGCTGCTTGAGTGTTTAGAAAACAATGGGTTGCATTCTGTTTGACCAGGGCTTACCTCAAGTACTCTATCCAAAGAAAAAAAAGAACAATCAGTGCAATCAGCAAAATTTGT > NC_002947/5552768‑5553058 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |