| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,607,908 | G→A | 7.8% | L77L (CTC→CTT) | cyaB ← | cyclolysin secretion/processing ATP‑binding protein CyaB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,607,908 | 0 | G | A | 7.8% | 257.3 / 8.7 | 89 | L77L (CTC→CTT) | cyaB | cyclolysin secretion/processing ATP‑binding protein CyaB |
| Reads supporting (aligned to +/- strand): ref base G (46/36); new base A (3/4); total (49/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01 | |||||||||||
CGGCGCGCGGGAATGGGGGTCCTGCACCAGAACCTGCTCGCCCTCCACGCGCGCGATGATGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCATGGTAACGCGCCAGCGTGATAAGACACACCAGCCCACTTTCAA > NC_002947/5607761‑5608053 | cggcgCGCGGGAATGGGGGTCCTGCACCAGAACCTGCTCGCCCTCCACGCGCGCGATGATGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGa < 1:357516/149‑1 (MQ=255) ggcgCGCGGGAATGGGGGTCCTGCACCAGAACCTGCTCGCCCTCCACGCGCGCGATGATGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAg > 4:244272/1‑149 (MQ=255) gcgcgcGGGAATGGGGGTCCTGCACCAGAACCTGCTCGCCCTCCACGCGCGCGATGATGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAgg > 5:83772/1‑149 (MQ=255) gcgcgGGAATGTGGGTCCTGCACCAGAACCTGCTCGCCCTCCACGCGCGCGATGATGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCTAGGGCGTCTGCTGCAGCC‑GGGCCGTATCGACGCTGCGGTGTTTGGCCTTGAGGcc < 1:335241/149‑1 (MQ=255) gggAATGGGGGTCCTGCACCAGAACCTGCTCGCCCTCCACGCGCGCGATGATGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGt > 5:271577/1‑149 (MQ=255) ggAATGGGGGTCCTGCACCAGAACCTGCTCGCCCTCCACGCGCGCGATGATGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGtt < 5:249408/149‑1 (MQ=255) gAATGGGGGTCCTGCACCAGAACCTGCTCGCCCTCCACGCGCGCGATGATGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTg > 4:375576/1‑149 (MQ=255) gAATGGGGGTCCTGCACCAGAACCTGCTCGCCCTCCACGCGCGCGATGATGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTCAAGGCCaaacac < 2:126915/149‑5 (MQ=255) gAATGGGGGTCCTGCACCAGAACCTGCTCGCCCTCCACGCGCGCGATGATGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTCAAGGCCaaacac < 5:249402/149‑5 (MQ=255) aaTGGGGGTCCTGCACCAGAACCTGCTCGCCCTCCACGCGCGCGATGATGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGc < 4:25553/149‑1 (MQ=255) aaTGGGGGTCCTGCACCAGAACCTGCTCGCCCTCCACGCAGGCGATGATGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTCAAGGCCaaacacc < 1:147590/149‑6 (MQ=255) ggTCCTGCACCAGAACCTGCTCGCCCTCCACGCGCGCGATGATGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAAt < 1:178810/149‑1 (MQ=255) tCCTGCACCAGAACCTGCTCGCCCTCCACGCGCGCGATGATGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGc < 4:182685/149‑1 (MQ=255) cTGCACCAGAACCTGCTCGCCCTCCACGCGCGCGATGATGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGccc > 3:199030/1‑149 (MQ=255) tGCACCAGAACCTGCTCGCCCTCCACGCGCGCGATGATGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGccc > 8:244961/1‑148 (MQ=255) cAGAACCTGCTCGCCCTCCACGCGCGCGATGATGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAagta < 3:244272/149‑1 (MQ=255) gAACCTGCTCGCCCTCCACGCGCGCGATGATGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGc < 5:391972/131‑1 (MQ=255) gAACCTGCTCGCCCTCCACGCGCGCGATGATGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGc > 6:391972/1‑131 (MQ=255) gAACCTGCTCGCCCTCCACGCGCGCGATGATGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAagtagt > 7:122299/1‑149 (MQ=255) cTGCTCGCCCTCCACGCGCGCGATGATGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGc < 6:385487/149‑1 (MQ=255) cTCGCCCTCCACGCGCGCGATGATGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGcctg > 5:237612/1‑147 (MQ=255) tCGCCCTCCACGCGCGCGATGATGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGt > 2:183274/1‑149 (MQ=255) gCCCTCCACGCGCGCGATGATGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGt < 8:122299/147‑1 (MQ=255) cACGCGCGCGATGATGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGaaa < 1:383843/148‑1 (MQ=255) cACGCGCGCGATGATGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAAc < 3:375576/149‑1 (MQ=255) gatgatGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAACCGCTCACCCg > 4:284564/1‑149 (MQ=255) gatgatGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCg > 6:61800/1‑149 (MQ=255) gatgatGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCg > 5:109853/1‑149 (MQ=255) aaGTACGCACCACGCGTACCCACTGCACTAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCtgctgc < 3:313639/149‑1 (MQ=255) gTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCAt > 3:355600/1‑149 (MQ=255) gTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCAt > 2:46876/1‑149 (MQ=255) ataaccacGCGTATCCACTGCAAAAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGAAAGACGCTGCGGTCTTTGGCCTAGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCAACCGATGCTGCATGAt > 4:249682/4‑149 (MQ=255) tCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATc > 8:405604/1‑149 (MQ=255) accacGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCg < 4:211606/148‑1 (MQ=255) ccacGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGtg < 4:355600/149‑1 (MQ=255) gcgTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCCGGGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGtgctg > 1:90747/1‑149 (MQ=255) gcgTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGtgctgc < 4:339157/149‑1 (MQ=255) gcgTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGtgctg > 8:227043/1‑148 (MQ=255) tATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAAc < 2:90747/149‑1 (MQ=255) ccACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCgctg > 3:407728/1‑126 (MQ=255) ccACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCgctg < 4:407728/126‑1 (MQ=255) cACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCt > 8:347470/1‑149 (MQ=255) aCTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTc > 8:40689/1‑149 (MQ=255) aCTGCAATAGCGGGCAAGGGCGTCTGCTGAAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTc < 2:321616/149‑1 (MQ=255) tGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCgg > 1:405661/1‑149 (MQ=255) tGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCgg > 4:369418/1‑149 (MQ=255) tGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCgg > 7:364344/1‑149 (MQ=255) cAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCgg > 4:228940/1‑147 (MQ=255) cAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGc < 3:369418/148‑1 (MQ=255) ttAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTGACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCaga < 6:271577/148‑1 (MQ=255) tAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCaa > 1:365009/1‑136 (MQ=255) tAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCaa < 2:365009/136‑1 (MQ=255) tAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCa > 8:32727/1‑146 (MQ=255) tAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCa > 3:318702/1‑146 (MQ=255) ggCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACgg > 5:385215/1‑148 (MQ=255) ggCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGc < 2:405661/149‑1 (MQ=255) ggCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGc < 3:228940/149‑1 (MQ=255) aaGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCaa > 1:57425/1‑148 (MQ=255) aaGGGCGTCTGCTGCAGCC‑GGGCAGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCgc < 7:300012/107‑1 (MQ=255) aaGGGCGTCTGCTGCAGCC‑GGGCAGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCgc > 8:300012/1‑107 (MQ=255) gCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCa < 6:83772/149‑1 (MQ=255) tCTGCTGCAGCC‑GGGCCGGATCGACGATGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTTCAACTGCTCGGCAGAGACGGCAACACCATgg > 8:6783/1‑149 (MQ=255) ctgctgCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCATGGt < 4:318702/149‑1 (MQ=255) gcAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCATGGTAAcgc > 3:371619/1‑149 (MQ=255) cAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCATGGTAAcgcg > 2:178875/1‑149 (MQ=255) aGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGTTCGGCAGAGACGGCAACACCATGGTAACgcgc < 1:46876/149‑1 (MQ=255) cc‑gggccggATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCATGGTAACgcgc > 8:40519/1‑147 (MQ=255) c‑gggccggATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCATGGTAACGCGCCAg < 7:40689/149‑1 (MQ=255) ccggATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCATGGTAACGCGCCAGCGt > 7:58240/1‑148 (MQ=255) ccggATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCATGGTAACGCGCCAGCGTg < 5:61800/149‑1 (MQ=255) tCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCATGGTAACGCGCCAGCGTGAt > 8:287939/1‑146 (MQ=255) gACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCATGGTAACGCGCCAGCGTGATAAGa < 6:109853/148‑1 (MQ=255) gACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCATGGTAACGCGCCAGCGTGATAAGa < 4:199030/148‑1 (MQ=255) aCGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCATGGTAACGCGCCAGCGTGATAAGaca > 7:186228/1‑149 (MQ=255) aCGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCATGGTAACGCGCCAGCGTGATAAGaca > 3:306885/1‑149 (MQ=255) tGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCATGGTAACGCGCCAGCGTGATAAGacacac > 1:55622/1‑148 (MQ=255) cGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCATGGTAACGCGCCAGCGTGATAAGACACAcc < 4:306885/147‑1 (MQ=255) ggTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCATGGTAACGCGCCAGCGTGATAAGACACACCAGc > 2:233766/1‑149 (MQ=255) aacttGGCCTCAAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCATGGTAACGCGCCAGCGTGATAAGCCCCACCAGcc > 2:185464/4‑148 (MQ=255) aacttGGCCTCAAGGCCAAGTTGCTGAAATGCCAGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCATGGTATCGCGCCAGCGTGATATGGCCCACCAGcc > 8:404521/4‑148 (MQ=255) tttGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCATGGTAACGCGCCAGCGTGATAAGACACACCAGCCCAc > 5:185722/1‑149 (MQ=255) cttGGCCTCAAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCATGGTAACGCGCCAGCGTGATAAGACACACCAGCCCa > 6:243075/2‑148 (MQ=255) ggCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCATGGTAACGCGCCAGCGTGATAAGACACACCAGCCCACttt > 3:88006/1‑149 (MQ=255) ggCCTCAAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCATGGTAACGCGCCAGCGTGATAAGACACACCAGCCCACtt < 5:243075/148‑1 (MQ=255) gCCTTGAGGCGAAGTTGCTGAAATGCCCGAAGTAGTTGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCATGGTAACGCGCCAGCGTGATAAGACACACCAGCCCACttt < 3:249682/148‑1 (MQ=255) gCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCATGGTAACGCGCCAGCGTGATAAGACACACCAGCCCACttt < 7:40519/148‑1 (MQ=255) gCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCATGGTAACGCGCCAGCGTGATAAGACACACCAGCCCACttt < 2:57425/148‑1 (MQ=255) ccTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCATGGTAACGCGCCAGCGTGATAAGACACACCAGCCCACTTTc > 3:276415/1‑148 (MQ=255) cTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCATGGTAACGCGCCAGCGTGATAAGACACACCAGCCCACTTTCaa > 2:188466/1‑149 (MQ=255) | CGGCGCGCGGGAATGGGGGTCCTGCACCAGAACCTGCTCGCCCTCCACGCGCGCGATGATGAAGTACTCACCACGCGTATCCACTGCAATAGCGGGCAAGGGCGTCTGCTGCAGCC‑GGGCCGGATCGACGCTGCGGTGTTTGGCCTTGAGGCCAAGTTGCTGAAATGCCCGAAGTAGTGGGCCGGTGGAGAAACGCTCACCCGCTGCTGCATGATCGTGCTGCAACTGCTCGGCAGAGACGGCAACACCATGGTAACGCGCCAGCGTGATAAGACACACCAGCCCACTTTCAA > NC_002947/5607761‑5608053 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |