Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 471,066 T→C 5.0% intergenic (‑110/+18) PP_0386 ← / ← rpoD sensory box protein/RNA polymerase sigma 70 factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947471,0660TC 5.0% 292.8 / 3.3 99intergenic (‑110/+18)PP_0386/rpoDsensory box protein/RNA polymerase sigma 70 factor
Reads supporting (aligned to +/- strand):  ref base T (45/49);  new base C (3/2);  total (48/51)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

TCAGGATCAGCAACAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGG  >  NC_002947/470920‑471210
                                                                                                                                                  |                                                                                                                                                 
tCAGGATCAGCAACAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTgg                                                                                                                                                 >  2:290958/1‑149 (MQ=255)
 cAGGATCAGCAACAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTggg                                                                                                                                                >  4:221850/1‑149 (MQ=255)
  aGGATCAGCAACAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTggg                                                                                                                                                <  4:430445/148‑1 (MQ=255)
      tCAGCAACAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCggg                                                                                                                                           <  4:388577/149‑1 (MQ=255)
       cAGCAACAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCggg                                                                                                                                           <  4:103708/148‑1 (MQ=255)
        aGCAACAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGt                                                                                                                                         <  1:260375/149‑1 (MQ=255)
          ctaCAGAATGGCCGGCTTCTAGGTCATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGGAGGTGTGCTACTGAAGGCGGGCTTTATGTCACGGGCCAGTAAAGAAAAACCCCGGCCCAGGCAGGGGttt                                                                                                                                       <  4:112731/147‑1 (MQ=255)
            aCAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTg                                                                                                                                     >  6:289867/1‑149 (MQ=255)
            aCAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGCTCTCAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTAACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTg                                                                                                                                     >  5:104254/1‑149 (MQ=255)
             cAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTg                                                                                                                                     >  5:306961/1‑148 (MQ=255)
               gAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCCAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGtca                                                                                                                                  >  5:383388/1‑149 (MQ=255)
                  tGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGTGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGtcatca                                                                                                                               <  3:257690/149‑1 (MQ=255)
                  tGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGtcatca                                                                                                                               <  5:212873/149‑1 (MQ=255)
                       ggCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGtcatca                                                                                                                               <  6:48035/144‑1 (MQ=255)
                       ggCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGtcatca                                                                                                                               >  5:48035/1‑144 (MQ=255)
                        gCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCTACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACtcgtc                                                                                                                         <  3:339382/149‑1 (MQ=255)
                          ttCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACtcgtc                                                                                                                         >  4:36374/1‑147 (MQ=255)
                              aGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGaggaa                                                                                                                   <  6:306961/149‑1 (MQ=255)
                               ggACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGaggaag                                                                                                                  <  5:35877/149‑1 (MQ=255)
                               ggACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGaggaag                                                                                                                  <  7:324265/149‑1 (MQ=255)
                               ggACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGaggaag                                                                                                                  >  4:138129/1‑149 (MQ=255)
                               ggACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGaggaag                                                                                                                  >  1:365183/1‑149 (MQ=255)
                                gACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGaggaag                                                                                                                  >  7:321543/1‑148 (MQ=255)
                                  cATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAg                                                                                                               >  1:65022/1‑149 (MQ=255)
                                    tGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAgcg                                                                                                             <  4:166975/149‑1 (MQ=255)
                                    tGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAgcg                                                                                                             <  6:70500/149‑1 (MQ=255)
                                        aGCCTCGCACTCAGGTGGTCGGAAGTCCAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAgg                                                                                                         <  6:383388/149‑1 (MQ=255)
                                            tCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCt                                                                                                     >  2:247627/1‑149 (MQ=255)
                                              gCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCg                                                                                                   <  7:278560/149‑1 (MQ=255)
                                              gCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCg                                                                                                   >  7:322994/1‑149 (MQ=255)
                                              gCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCg                                                                                                   >  6:139168/1‑149 (MQ=255)
                                               cACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGc                                                                                                  >  1:188916/1‑149 (MQ=255)
                                                aCTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCg                                                                                                   <  6:394545/147‑1 (MQ=255)
                                                aCTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCg                                                                                                   >  5:394545/1‑147 (MQ=255)
                                                 cTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑g                                                                                               <  3:221850/149‑1 (MQ=255)
                                                    aGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGa                                                                                            <  4:428556/149‑1 (MQ=255)
                                                     ggtggtCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGa                                                                                            <  8:321543/148‑1 (MQ=255)
                                                     ggtggtCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAg                                                                                           <  7:72695/149‑1 (MQ=255)
                                                         gtCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAATAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCg                                                                                        <  4:361000/148‑1 (MQ=255)
                                                         gtCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCgg                                                                                       <  5:139168/149‑1 (MQ=255)
                                                          tCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCggg                                                                                      >  5:355608/1‑149 (MQ=255)
                                                           cTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGt                                                                                     >  5:91235/1‑149 (MQ=255)
                                                           cTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGt                                                                                     >  4:394665/1‑149 (MQ=255)
                                                            tGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACtcg                                                                                                                           >  3:409804/1‑111 (MQ=255)
                                                            tGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACtcg                                                                                                                           <  4:409804/111‑1 (MQ=255)
                                                            tGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTg                                                                                    >  6:235960/1‑149 (MQ=255)
                                                            tGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTg                                                                                    >  6:241999/1‑149 (MQ=255)
                                                              aaGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGt                                                                                     <  5:289867/146‑1 (MQ=255)
                                                              aaGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGgc                                                                                  <  8:92292/149‑1 (MQ=255)
                                                                gTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGgcg                                                                                 >  5:69009/1‑148 (MQ=255)
                                                                     gtgtAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCtt                                                                           >  3:26001/1‑149 (MQ=255)
                                                                        tAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTgc                                                                         <  6:151368/148‑1 (MQ=255)
                                                                           aCGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAgg                                                                                                         <  3:274190/114‑1 (MQ=255)
                                                                           aCGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAgg                                                                                                         >  4:274190/1‑114 (MQ=255)
                                                                            cGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAACAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAAc                                                                    <  7:344733/149‑1 (MQ=255)
                                                                             gggCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTAGCGAGTCGGGTGGCGCAGCTTGCGCAAc                                                                    <  1:247627/149‑1 (MQ=255)
                                                                             gggCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACg                                                                   <  2:24244/149‑1 (MQ=255)
                                                                              gtcATTGCCGCAGGTGAGCAACGGAAGGCTGGCTTTATGTCACGTGCCACTAAAGTAAAACGCCGGCCTGGGCCGGGGTTTTGTTATCACTCGTCGAGGAAGGAGCGCAGGTGATCGCT‑GCGATTCTGGTGGCGCAGCTTGCGCAACGc                                                                  <  8:314779/147‑1 (MQ=255)
                                                                                 aTTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCctt                                                               <  3:394665/149‑1 (MQ=255)
                                                                                  ttGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCcttc                                                              <  3:229304/149‑1 (MQ=255)
                                                                                      cGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCcttcgctt                                                          <  6:222676/149‑1 (MQ=255)
                                                                                      cGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCcttcgctt                                                          >  5:252208/1‑149 (MQ=255)
                                                                                      cGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCcttcgctt                                                          >  4:369288/1‑149 (MQ=255)
                                                                                      cGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCcttcgctt                                                          <  1:290958/149‑1 (MQ=255)
                                                                                        cAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCcttcgcttcg                                                        <  6:252208/149‑1 (MQ=255)
                                                                                               gCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGAc                                                 >  7:177987/1‑149 (MQ=255)
                                                                                                cAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGAc                                                 <  2:65022/148‑1 (MQ=255)
                                                                                                cAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACg                                                >  6:266752/1‑149 (MQ=255)
                                                                                                 aaCGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTg                                                                          <  1:313553/122‑1 (MQ=255)
                                                                                                 aaCGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTg                                                                          >  2:313553/1‑122 (MQ=255)
                                                                                                  aCGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGa                                              >  2:263560/1‑149 (MQ=255)
                                                                                                    ggAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATc                                            <  5:241999/149‑1 (MQ=255)
                                                                                                    ggAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATc                                            <  5:235960/149‑1 (MQ=255)
                                                                                                    ggAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATc                                            <  3:36374/149‑1 (MQ=255)
                                                                                                       aGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGc                                         <  2:365183/149‑1 (MQ=255)
                                                                                                       aGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGc                                         >  7:276326/1‑149 (MQ=255)
                                                                                                         gCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCt                                        >  1:217035/1‑148 (MQ=255)
                                                                                                                           gCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCcttcgcttcg                                                        <  6:337487/114‑1 (MQ=255)
                                                                                                                           gCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCcttcgcttcg                                                        >  5:337487/1‑114 (MQ=255)
                                                                                                                              aCTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCtt                  <  6:104254/149‑1 (MQ=255)
                                                                                                                               cTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTg                 >  1:45227/1‑149 (MQ=255)
                                                                                                                               cTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACACCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTg                 >  5:59502/1‑149 (MQ=255)
                                                                                                                                tAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGc                >  1:242621/1‑149 (MQ=255)
                                                                                                                                tAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGc                >  7:12484/1‑149 (MQ=255)
                                                                                                                                  aaGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCCCGCGTTACGTCAACCTGCTTGccc              >  8:400734/1‑149 (MQ=255)
                                                                                                                                  aaGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGccc              <  3:138129/149‑1 (MQ=255)
                                                                                                                                    gAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCAc            >  8:194930/1‑149 (MQ=255)
                                                                                                                                     caaaaCCCCGGCCCAGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCAcc           >  6:189734/2‑149 (MQ=255)
                                                                                                                                       aaaCCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACctc         <  4:26001/149‑1 (MQ=255)
                                                                                                                                        aaCCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACctct        >  8:404919/1‑149 (MQ=255)
                                                                                                                                        aaCCCCGGCCCAGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACctct        >  3:366583/1‑149 (MQ=255)
                                                                                                                                         aCCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCtt       <  1:263560/149‑1 (MQ=255)
                                                                                                                                          ccccGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTc      >  3:74449/1‑149 (MQ=255)
                                                                                                                                          ccccGGCCCAGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTc      >  5:106930/1‑149 (MQ=255)
                                                                                                                                          ccccGGCCCAGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTc      <  5:189734/149‑1 (MQ=255)
                                                                                                                                           cccGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTc      >  4:390064/1‑148 (MQ=255)
                                                                                                                                             cGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGccc              <  5:264874/138‑1 (MQ=255)
                                                                                                                                             cGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGccc              >  6:264874/1‑138 (MQ=255)
                                                                                                                                              ggCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAgg  <  5:266752/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TCAGGATCAGCAACAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCT‑GCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGG  >  NC_002947/470920‑471210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑