Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,743,882	A→G	5.4%	G661G (GGT→GGC)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,743,882	0	A	G	5.4%	216.1 / 2.1	74	G661G (GGT→GGC)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base A (35/34);  new base G (2/2);  total (38/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.32e-01

GGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGG  >  NC_002947/5743735‑5744027                                                                                                                                                    |                                                                                                                                                  ggCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTAc                                                                                                                                                  >  2:24139/1‑149 (MQ=255) ggCACGCTTGCGCGCTTCAACCTGCGGCGAGGTCACGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTAc                                                                                                                                                  <  1:405028/149‑1 (MQ=255)    aCGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCAGGTTGCTGGTACCg                                                                                                                                                >  7:196843/1‑148 (MQ=255)     cGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGa                                                                                                                                              <  4:413341/149‑1 (MQ=255)          gcgcgcTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCt                                                                                                                                          <  1:393652/148‑1 (MQ=255)           cgcgcTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTc                                                                                                                                        <  5:136661/149‑1 (MQ=255)            gcgcTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCCAACTTCATGTTGCTGGTACCGGAAGCTTCg                                                                                                                                       <  1:39134/149‑1 (MQ=255)              gcTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTGCCGGAAGCTTCGt                                                                                                                                      >  3:368363/1‑148 (MQ=255)              gcTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTGCCGGAAGCTTCGt                                                                                                                                      >  3:362384/1‑148 (MQ=255)              gcTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTa                                                                                                                                     <  4:201830/149‑1 (MQ=255)                ttCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAAc                                                                                                                                   >  6:336344/1‑149 (MQ=255)                  cAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGCGGCCGAACTTCTTGTTGCTGGTCCCGGAAGCTTGGTAAccc                                                                                                                                 >  6:327363/1‑149 (MQ=255)                   aaCCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGCACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCcg                                                                                                                                >  6:144344/1‑149 (MQ=255)                    aCCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCcgc                                                                                                                               >  8:362111/1‑149 (MQ=255)                      ctgctgCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCgg                                                                                                                             >  5:278583/1‑149 (MQ=255)                         ctgcGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGt                                                                                                                            <  5:187387/147‑1 (MQ=255)                              aGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATc                                                                                                                     >  5:184080/1‑149 (MQ=255)                               ggTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCt                                                                                                                    <  1:205658/149‑1 (MQ=255)                                   aGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTGCCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTc                                                                                                                <  4:362384/149‑1 (MQ=255)                                   aGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTGCCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTc                                                                                                                <  4:368363/149‑1 (MQ=255)                                   aGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTc                                                                                                                <  5:310239/149‑1 (MQ=255)                                      ccAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCgg                                                                                                              >  7:200505/1‑148 (MQ=255)                                             tGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTcc                                                                                                      <  8:200505/149‑1 (MQ=255)                                              gAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTccg                                                                                                     >  7:406146/1‑149 (MQ=255)                                                 cATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCcg                                                                                                                                >  6:372537/1‑119 (MQ=255)                                                 cATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCcg                                                                                                                                <  5:372537/119‑1 (MQ=255)                                                       gtCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGGGGATATCTGCTCGGAAAGGTCCGCCGCCGGga                                                                                            >  4:22040/1‑149 (MQ=255)                                                        tCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGga                                                                                            <  3:62959/148‑1 (MQ=255)                                                         cGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGgat                                                                                           >  4:263812/1‑148 (MQ=255)                                                           gCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGgatgat                                                                                        >  4:395346/1‑149 (MQ=255)                                                            cccccACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGgatgat                                                                                        >  8:209827/1‑148 (MQ=255)                                                            cccccACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGatgatg                                                                                       <  5:210251/149‑1 (MQ=255)                                                             ccccACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGc                                                                                      >  8:281022/1‑149 (MQ=255)                                                                   cTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCgg                                                                                <  7:362111/149‑1 (MQ=255)                                                                        cGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAgg                                                                           >  7:71243/1‑149 (MQ=255)                                                                         gCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGc                                                                          >  1:362431/1‑149 (MQ=255)                                                                           acaTCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTg                                                                        <  1:11400/149‑1 (MQ=255)                                                                                tcCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGtt                                                                   >  7:334092/1‑149 (MQ=255)                                                                                  cACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTg                                                                  <  2:154028/148‑1 (MQ=255)                                                                                  cACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTg                                                                  <  3:263812/148‑1 (MQ=255)                                                                                  cACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGt                                                                 >  8:126969/1‑149 (MQ=255)                                                                                   aCGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTACATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTg                                                                  >  4:420911/1‑147 (MQ=255)                                                                                    cGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAg                                                               <  6:184080/149‑1 (MQ=255)                                                                                    cGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAg                                                               <  5:88815/149‑1 (MQ=255)                                                                                     gtaggCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGTCCGAACTTCATGTTGCTCGTAAAGGAAGCTTCGTAACCAGCGGTGGATATCTGCTCGGATACGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTagtagt                                                              >  5:304225/4‑149 (MQ=255)                                                                                     gTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGt                                                              <  1:24139/149‑1 (MQ=255)                                                                                        ggCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTg                                                            <  3:395346/148‑1 (MQ=255)                                                                                          cgcCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTggg                                                          >  3:222021/1‑148 (MQ=255)                                                                                          cgcCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGc                                                         <  8:406146/149‑1 (MQ=255)                                                                                          cgcCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGc                                                         <  7:209827/149‑1 (MQ=255)                                                                                             cATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCt                                                                         <  7:155198/130‑1 (MQ=255)                                                                                             cATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCt                                                                         >  8:155198/1‑130 (MQ=255)                                                                                                 cAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAac                                                   <  6:278583/148‑1 (MQ=255)                                                                                                    ggTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAAcacca                                               >  8:385776/1‑149 (MQ=255)                                                                                                      ttccAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACaccacc                                             <  7:360937/147‑1 (MQ=255)                                                                                                       ctcAATGGTGCGCTCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCt                                            >  8:409442/3‑149 (MQ=255)                                                                                                         cAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCtt                                           >  7:257116/1‑148 (MQ=255)                                                                                                                   cgcACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTTGTAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCTTCAGCAGGCcgc                                <  7:281022/149‑1 (MQ=255)                                                                                                                    gcACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGCCGTTGTAGTTGGGCAGGAACACAACCTTCAGCAGGCcgcg                               >  8:159264/1‑149 (MQ=255)                                                                                                                     cACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACg                                                                     >  6:17881/1‑110 (MQ=255)                                                                                                                     cACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACg                                                                     <  5:17881/110‑1 (MQ=255)                                                                                                                        cATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCTTCAGCAGGCCGCGCACt                           <  5:336344/149‑1 (MQ=255)                                                                                                                         aTTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTAGGAAAGGTCCGCAGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCTTAAGCAGGCCGCTCACTg                          >  4:244199/1‑149 (MQ=255)                                                                                                                          ttGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCTTCAGCAGGCCGCGCACTGt                         >  8:122621/1‑149 (MQ=255)                                                                                                                             atggcGAACTTCTCGTGGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATTTCTGCTCGGAATGGTCCGCCGCCTGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCTTCAGCAGGCCGCTCACTGTCg                       <  6:304225/144‑1 (MQ=255)                                                                                                                                 cGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCg                  >  8:386540/1‑149 (MQ=255)                                                                                                                                   aaCTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCgtt                <  4:226308/149‑1 (MQ=255)                                                                                                                                    aCTTCATGTGGCTGGTACCGGAAGCTTCGGACCCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGGCgttg               <  1:261910/149‑1 (MQ=255)                                                                                                                                         aTGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCTTCAGCAGGCCGCGCGCTGTCGGGTCGTTGTTcac          >  5:413646/1‑149 (MQ=255)                                                                                                                                         aTGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTcac          >  7:90143/1‑149 (MQ=255)                                                                                                                                           gTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTcacca        >  3:150197/1‑149 (MQ=255)                                                                                                                                           gTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTcacca        >  7:27297/1‑149 (MQ=255)                                                                                                                                                  gTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCggg  <  8:27297/148‑1 (MQ=255)                                                                                                                                                  gTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCgg   <  2:362431/147‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  GGCACGCTTGCGCGCTTCAACCTGCTGCGAGGTCAGGCCAAAGATGAACATGTTGTCGGCCCCCACCTGTTCGCACATCTCCACGTTGGCGCCATCCAGGGTGCCAATGGTGAGCGCACCATTGAGGCCGAACTTCATGTTGCTGGTACCGGAAGCTTCGTAACCCGCGGTGGATATCTGCTCGGAAAGGTCCGCCGCCGGGATGATGCTCTCGGCCAGGCTGACGTTGTAGTTGGGCAGGAACACCACCTTCAGCAGGCCGCGCACTGTCGGGTCGTTGTTCACCACCCGGG  >  NC_002947/5743735‑5744027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑