BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,763,633	G→C	5.3%	A337P (GCC→CCC)	PP_5057 →	M23/M37 family peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,763,633	0	G	C	5.3%	371.5 / 8.3	113	A337P (GCC→CCC)	PP_5057	M23/M37 family peptidase
Reads supporting (aligned to +/- strand): ref base G (53/54); new base C (2/4); total (55/58)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.60e-01

Only 100 of 114 total aligned reads displayed.

AAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTA  >  NC_002947/5763485‑5763778

aaaGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTg                                                                                                                                                   <  1:11615/149‑1 (MQ=255)

 aacgCCCGTATCCCCCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGc                                                                                                                                                  <  5:48915/146‑1 (MQ=255)

   ggCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGcc                                                                                                                                                 >  1:380998/1‑148 (MQ=255)

    gCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGcc                                                                                                                                                 >  6:364189/1‑147 (MQ=255)

      ccGTACCACCCTCGGGCCTATGGTTTCCAGCGATGGCGCAAACTACGGCGGCGAATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGTTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGt                                                                                                                                             <  3:224310/149‑1 (MQ=255)

      ccGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGcc                                                                                                                                                 <  3:224887/145‑1 (MQ=255)

      ccGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGcc                                                                                                                                                 >  4:224887/1‑145 (MQ=255)

      ccGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGt                                                                                                                                             <  5:59239/149‑1 (MQ=255)

      ccGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGt                                                                                                                                             <  5:88449/149‑1 (MQ=255)

           ccaccCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGa                                                                                                                                         <  2:252516/148‑1 (MQ=255)

            caccCTCGGGCCGATGGTTTCCAGCGCTGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGGCAGTCTATGGTCGATTGCTGGCACGTGTCGGTGATGCCCGCGGTGGCGACGCGCGTGCCAAGGGGGACg                                                                                                                                       >  8:330250/1‑149 (MQ=255)

            caccCTCGGGCCGATGGTTTCCAGCCATGGCGCCAAATACGGCGGCGCATTTTCTGATGCGCGTGGAAAACTTCCATGGCAAGTCAATGGTCGATTGCTGCCACGTTTCGGTGATGCCCGCTGTAGCGACGCCCGTGCCAAGTGGCACg                                                                                                                                       >  2:374021/1‑149 (MQ=255)

              cccTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACggg                                                                                                                                     <  5:430324/149‑1 (MQ=255)

              cccTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACggg                                                                                                                                     >  6:314876/1‑149 (MQ=255)

              cccTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTCCCACTTGGCACggg                                                                                                                                     <  3:98222/149‑1 (MQ=255)

               ccTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACgggg                                                                                                                                    <  4:252197/149‑1 (MQ=255)

               ccTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTCCCACTTGGCACgggc                                                                                                                                    <  6:78241/149‑2 (MQ=255)

                cTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGTAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGACCCACTTGGCACgggcg                                                                                                                                   <  5:44345/149‑3 (MQ=255)

                 tCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGt                                                                                                                                   >  6:252248/1‑148 (MQ=255)

                 tCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGt                                                                                                                                   >  5:353980/1‑148 (MQ=255)

                    ggCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTCCCACTTGGCACgggcgtcgc                                                                                                                               <  3:139357/149‑7 (MQ=255)

                     gCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGa                                                                                                                              >  2:266412/1‑149 (MQ=255)

                       cGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGAGGCCCGTGCCAAGTGGGACGGGGTTATGATc                                                                                                                            <  1:38934/149‑1 (MQ=255)

                       cGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGAt                                                                                                                             >  8:225442/1‑148 (MQ=255)

                       cGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATc                                                                                                                            <  7:88884/149‑1 (MQ=255)

                            gTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAgcgc                                                                                                                       >  1:281422/1‑149 (MQ=255)

                               tCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCaa                                                                                                                    >  8:373218/1‑149 (MQ=255)

                                ccAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAAc                                                                                                                   >  3:27714/1‑149 (MQ=255)

                                  aGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAAccc                                                                                                                 >  7:218412/1‑149 (MQ=255)

                                  aGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAAccc                                                                                                                 >  7:212307/1‑149 (MQ=255)

                                  aGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAAcc                                                                                                                  >  5:169945/1‑148 (MQ=255)

                                   gCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAAcc                                                                                                                  >  8:378734/1‑147 (MQ=255)

                                   gCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCa                                                                                                                <  5:271487/149‑1 (MQ=255)

                                      aTGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCAAgg                                                                                                              >  3:47922/1‑148 (MQ=255)

                                               aCTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTa                                                                                                    <  8:218412/149‑1 (MQ=255)

                                               aCTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTa                                                                                                    >  5:124792/1‑149 (MQ=255)

                                               aCTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTa                                                                                                    <  8:212307/149‑1 (MQ=255)

                                                cTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCCGGCACCCAGGTAc                                                                                                   <  4:349565/149‑1 (MQ=255)

                                                   cggcggCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCAGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTAcgt                                                                                                 <  4:47922/148‑1 (MQ=255)

                                                    ggcggcGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTAcgtgc                                                                                               >  8:119588/1‑149 (MQ=255)

                                                      cggcgCATTTTCTGCTGCGCGGGGCAAACTTCAATGTCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTAcgtgccg                                                                                             <  7:302020/149‑1 (MQ=255)

                                                       ggcgCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTAcgtgccgt                                                                                            >  5:124970/1‑149 (MQ=255)

                                                        gcgcATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTAcgtgccgtg                                                                                           <  6:365735/149‑1 (MQ=255)

                                                          gcATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCa                                                                                         >  1:64118/1‑149 (MQ=255)

                                                          gcATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCa                                                                                         >  1:57822/1‑149 (MQ=255)

                                                          gcATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGCTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCa                                                                                         <  7:395239/149‑1 (MQ=255)

                                                             ttttCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCAcgg                                                                                      <  5:314876/149‑1 (MQ=255)

                                                                       cgcgGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCgtgt                                                                             <  1:266412/148‑1 (MQ=255)

                                                                       cgcgGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGtgtg                                                                            <  5:364189/149‑1 (MQ=255)

                                                                        gcgGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTgg                                                                           <  4:247862/149‑1 (MQ=255)

                                                                        gcgGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTgg                                                                           <  8:64008/149‑1 (MQ=255)

                                                                         cgGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTgg                                                                           >  6:216378/1‑148 (MQ=255)

                                                                          ggggCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTa                                                                         >  7:350841/1‑149 (MQ=255)

                                                                           gggCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTAt                                                                        >  1:413051/1‑149 (MQ=255)

                                                                                 aCTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCgg                                                                  <  5:216378/149‑1 (MQ=255)

                                                                                   ttCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGAc                                                                <  2:281422/149‑1 (MQ=255)

                                                                                     ccATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGAc                                                                <  7:242165/147‑1 (MQ=255)

                                                                                      cATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTgg                                                             <  6:124970/149‑1 (MQ=255)

                                                                                      cATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTg                                                              <  6:432657/148‑1 (MQ=255)

                                                                                       aTGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCTGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGt                                                            >  3:360967/1‑149 (MQ=255)

                                                                                          gCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGtgtg                                                                            >  7:316462/1‑130 (MQ=255)

                                                                                          gCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGtgtg                                                                            <  8:316462/130‑1 (MQ=255)

                                                                                             aGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGcgcg                                                      <  7:373218/149‑1 (MQ=255)

                                                                                             aGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGcgcg                                                      <  2:64118/149‑1 (MQ=255)

                                                                                             aGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGcgcg                                                      <  2:57822/149‑1 (MQ=255)

                                                                                                 aaTGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTg                                                   <  2:413051/148‑1 (MQ=255)

                                                                                                 aaTGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGc                                                  >  8:404786/1‑149 (MQ=255)

                                                                                                    ggTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCgg                                               >  5:243729/1‑149 (MQ=255)

                                                                                                      tCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGc                                             <  7:119588/149‑1 (MQ=255)

                                                                                                        gATTGCTGGCACGTTTCGGTGATGCCCGCGGGAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCtt                                           >  1:267650/1‑149 (MQ=255)

                                                                                                         aTTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTc                                          >  7:144915/1‑149 (MQ=255)

                                                                                                            gCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACATGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTgg                                       >  4:88741/1‑149 (MQ=255)

                                                                                                             cTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGCGGTATGATCAGCGCCAACCCAGGCACCCACGTACGTGCCGAGCCCGGCGGGCGTGAGGTATTAGCGGACTGGTTGCGTGGTGCAGGGCTTCTGGt                                      >  2:424735/1‑149 (MQ=255)

                                                                                                              tGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTc                                          >  1:267178/1‑144 (MQ=255)

                                                                                                              tGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTc                                          <  2:267178/144‑1 (MQ=255)

                                                                                                              tGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGt                                      >  5:387549/1‑148 (MQ=255)

                                                                                                                gCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCa                                    <  1:57757/148‑1 (MQ=255)

                                                                                                                 cACGTTTCGGTGATGCCCGCGGTAGCGACGGCCGTGCCCAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCAtt                                  <  3:314808/149‑1 (MQ=255)

                                                                                                                  aCGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGATATCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATtc                                 >  1:363399/1‑149 (MQ=255)

                                                                                                                    gTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTc                                          >  1:105647/1‑138 (MQ=255)

                                                                                                                           tGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATg                        <  6:169945/149‑1 (MQ=255)

                                                                                                                            gATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAAGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATgg                       >  2:341199/1‑149 (MQ=255)

                                                                                                                             aTGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCATGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATgg                       <  1:341199/148‑1 (MQ=255)

                                                                                                                               gCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTc                                     <  8:80000/132‑1 (MQ=255)

                                                                                                                               gCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCaa                    >  4:275182/1‑149 (MQ=255)

                                                                                                                                cccGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATgg                       <  1:70136/145‑1 (MQ=255)

                                                                                                                                cccGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATgg                       >  2:70136/1‑145 (MQ=255)

                                                                                                                                  cgcgGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACgg                 >  7:87568/1‑149 (MQ=255)

                                                                                                                                  cgcgGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACgg                 >  8:143321/1‑149 (MQ=255)

                                                                                                                                   gcgGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGt                <  7:378734/149‑1 (MQ=255)

                                                                                                                                    cgGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTTTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGtt               <  4:27714/149‑1 (MQ=255)

                                                                                                                                      gTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGTGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTAc             <  7:143321/149‑1 (MQ=255)

                                                                                                                                          cGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCt           >  3:101348/1‑147 (MQ=255)

                                                                                                                                          ataaccccGTCCCACTTGGCACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCt           >  6:393121/6‑147 (MQ=255)

                                                                                                                                           taaccccGTCCCACTTGGCACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAg        >  6:222303/5‑149 (MQ=255)

                                                                                                                                           gACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAg        >  2:318966/1‑149 (MQ=255)

                                                                                                                                            aCGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGc       <  6:57509/149‑1 (MQ=255)

                                                                                                                                            aCGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGc       >  6:36973/1‑149 (MQ=255)

                                                                                                                                              gCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCt     <  6:124792/149‑1 (MQ=255)

                                                                                                                                                 cGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACATGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTa  <  3:88741/149‑1 (MQ=255)

AAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTGCTGCGCGGGGCAAACTTCCATGGCCAGTCAATGGTCGATTGCTGGCACGTTTCGGTGATGCCCGCGGTAGCGACGCCCGTGCCAAGTGGGACGGGGTTATGATCAGCGCCAACCCAGGCACCCAGGTACGTGCCGTGCACGGCGGGCGTGTGGTATTCGCGGACTGGTTGCGCGGTGCCGGGCTTCTGGTCATTCTCGACCATGGCAACGGTTACCTGAGCCTGTA  >  NC_002947/5763485‑5763778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`