Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,772,152	G→T	5.4%	L271M (CTG→ATG)	betT‑I ←	choline transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,772,152	0	G	T	5.4%	240.5 / 3.2	74	L271M (CTG→ATG)	betT‑I	choline transporter
Reads supporting (aligned to +/- strand):  ref base G (36/34);  new base T (2/2);  total (38/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

ACTTGCCGGCTTCGCCTTCATACACATAGAGGTCGAAGGTTTTCAGCACGATGCCGTTCAGGTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATCACCACCAGCAGGTTGGTCTTGCTGTGGTCCATGCCGAACAGGTATTCCAGGCCGGACGA  >  NC_002947/5772005‑5772299                                                                                                                                                    |                                                                                                                                                    aCTTGCCGGCTTCGCCTTCATACACATAGAGGTCGAAGGTTTTCAGCACGATGCCGTTCAGGTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGc                                                                                                                                                    <  5:418044/149‑1 (MQ=255)      ccGGCTTCGCCTTCATACACATAGAGGTCGAAGGTTTTCAGCACGATGCCGTTCAGGTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCTCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCatgat                                                                                                                                               >  7:179523/1‑149 (MQ=255)           ttCGCCTTCATACACATAGAGGTCGAAGGTTTTCAGTACGATGCCGTTCAGGTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGtt                                                                                                                                            >  1:201146/1‑147 (MQ=255)             cGCCTTCCTACACATAGAGGGCGAAGGTTTTCAGCACGATGCCGTTCAGGTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAg                                                                                                                                         <  6:409864/148‑1 (MQ=255)             cGCCTTCATACACATAGAGGTCGAAGGTTTTCAGCACGATGCCGTTCAGGTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCAACTGAACATCATGCTGTTCAg                                                                                                                                         <  1:155474/148‑1 (MQ=255)              gCCTTCATACACATAGAGGTCGAAGGTTTTCAGCACGATGCCGTTCAGGTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACATCATGCTGTTCAgtg                                                                                                                                       <  6:360533/149‑3 (MQ=255)               ccTTCATACACATAGAGGTCGAAGGTTTTCAGCACGATGCCGTTCAGGTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGtt                                                                                                                                      <  1:157231/149‑1 (MQ=255)                    aTACACATAGAGGTCGAAGGTTTTCAGCACGATGCCGTTCAGGTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGa                                                                                                                                   >  6:150212/1‑147 (MQ=255)                     tACACATAGAGGTCGAAGGTTTTCAGCACGATGCCGTTCAGGTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAg                                                                                                                                >  2:40003/1‑149 (MQ=255)                      acacATAGAGGTCGAAGGTTTTCAGCACGATGCCGTTCAGGTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAgg                                                                                                                               >  1:165158/1‑149 (MQ=255)                       cacaTAGAGGTCGAAGGTTTTCAGCACGATGCCGTTCAGGTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAggc                                                                                                                              <  4:227012/149‑1 (MQ=255)                        acaTAGAGGTCGAAGGTTTTCAGCACGATGCCGTTCAGGTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGtt                                                                                                                                      >  1:173914/1‑140 (MQ=255)                          aTAGAGGTCGAAGGTTTTCAGCACGATGCCGTTCAGGTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAggcggc                                                                                                                           >  6:101672/1‑149 (MQ=255)                          aTAGAGGTCGAAGGTTTTCAGCACGATGCCGTTCAGGTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAggcggc                                                                                                                           >  1:15291/1‑149 (MQ=255)                                gTCGAAGGTTTTCAGCACGATGCCGTTCAGGTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGCTCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGc                                                                                                                     >  1:311746/1‑149 (MQ=255)                                gTCGAAGGTTTTCAGCACGATGCCGTTCAGGTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGc                                                                                                                     >  1:427116/1‑149 (MQ=255)                                 tCGAAGGTTTTCAGCACGATGCCGTTCAGGTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGcc                                                                                                                    <  8:147001/149‑1 (MQ=255)                                 tCGAAGGTTTTCAGCACGATGCCGTTCAGGTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGcc                                                                                                                    <  5:101672/149‑1 (MQ=255)                                   gAAGGTTTTCAGCACGATGCCGTTCAGGTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCg                                                                                                                   <  2:427116/148‑1 (MQ=255)                                        ttttCAGCACGATGCCGTTCAGGTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTtctc                                                                                                              >  7:187001/1‑148 (MQ=255)                                        ttttCAGCACGATGCCGTTCAGGTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTcc                                                                                                             <  1:22424/149‑1 (MQ=255)                                             aGCACGATGCCGTTCAGGTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGcc                                                                                                        >  2:386356/1‑149 (MQ=255)                                                    tGCCGTTCAGGTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATAcc                                                                                                 >  6:199809/1‑149 (MQ=255)                                                       cGTTCAGGTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCt                                                                                              >  2:328433/1‑149 (MQ=255)                                                         ttCAGGTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGc                                                                                            <  5:249326/149‑1 (MQ=255)                                                         ttCAGGTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGc                                                                                            <  2:201146/149‑1 (MQ=255)                                                              gTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACgg                                                                                       >  4:327746/1‑149 (MQ=255)                                                               tAGTCGCCAACGTGCTGCACGACGCCGTTGAGCAGGTGCAGGGGTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGt                                                                                      <  1:356046/149‑1 (MQ=255)                                                                 gTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTa                                                                                     <  3:50204/148‑1 (MQ=255)                                                                 gTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTa                                                                                     <  5:150212/148‑1 (MQ=255)                                                                    gCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCa                                                                                 >  2:223738/1‑149 (MQ=255)                                                                       aaCGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGc                                                                              <  2:165158/149‑1 (MQ=255)                                                                             cTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGc                                                                                   >  1:35891/1‑138 (MQ=255)                                                                             cTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGc                                                                                   <  2:35891/138‑1 (MQ=255)                                                                                cACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCAt                                                                       <  8:179523/147‑1 (MQ=255)                                                                                cACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGa                                                                     >  5:64153/1‑149 (MQ=255)                                                                                   gAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGAc                                                                    >  1:380907/1‑147 (MQ=255)                                                                                    aaGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAg                                                                 >  7:58554/1‑149 (MQ=255)                                                                                       ccGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGAt                                                              <  6:204541/149‑1 (MQ=255)                                                                                         gTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATca                                                            >  3:170010/1‑149 (MQ=255)                                                                                             aGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATcaccac                                                        >  3:806/1‑149 (MQ=255)                                                                                                     cAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGAc                                                                    <  8:8396/129‑1 (MQ=255)                                                                                                     cAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGAc                                                                    >  7:8396/1‑129 (MQ=255)                                                                                                         gTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGTAGAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATCACGACCAGCAGGTTGGt                                            <  2:403241/149‑1 (MQ=255)                                                                                                         gTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATCACCACCAGCAGGTTGGt                                            >  2:75641/1‑149 (MQ=255)                                                                                                          tttCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGa                                                               <  8:146831/129‑1 (MQ=255)                                                                                                          tttCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGa                                                               >  7:146831/1‑129 (MQ=255)                                                                                                          tttCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATCACCACCAGCAGGTTGGTc                                           >  8:36047/1‑149 (MQ=255)                                                                                                                  cccAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACTGTAGCCACGCCGGCCATGACCAGGATCACCACCAGCAGGTTGGTCTTGCtgtg                                   <  2:15291/149‑1 (MQ=255)                                                                                                                   ccAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATCACCACCAGCAGGTTGGTCTTGCTGTgg                                  >  6:279189/1‑149 (MQ=255)                                                                                                                       cagcaCGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATCACCACCAGCAGGTTGGTCTTGCTGTGGTCCa                              >  5:292938/1‑149 (MQ=255)                                                                                                                        agcaCGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATCACCACCAGCAGGTTGGTCTTGCTGTGGTCCAt                             <  1:386356/149‑1 (MQ=255)                                                                                                                         gcaCGAAGCTCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATCACCACCAGCAGGTTGGTCTTGCTGTGGTCCATg                            <  2:311746/149‑1 (MQ=255)                                                                                                                         gcaCGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATCACCACCAGCAGGTTGGTCTTGCTGTGGTCCATg                            <  8:58554/149‑1 (MQ=255)                                                                                                                          caCGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATCACCACCAGCAGGTTGGTCTTGCTGTGGTCCATGc                           >  5:177185/1‑149 (MQ=255)                                                                                                                           aCGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATCACCACCAGCAGGTTGGTCTTGCTGTGGTCCATGcc                          <  1:75641/149‑1 (MQ=255)                                                                                                                             gAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATcaccac                                                        <  6:74134/117‑1 (MQ=255)                                                                                                                             gAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATcaccac                                                        >  5:74134/1‑117 (MQ=255)                                                                                                                               aGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATCACCACCAGCAGGTTGGTCTTGCTGTGGTCCATGCCGAAc                      <  2:380907/149‑1 (MQ=255)                                                                                                                                gATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATCACCACCAGCAGGTTGGTCTTGCTGTGGTCCATg                            <  5:238970/142‑1 (MQ=255)                                                                                                                                gATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATCACCACCAGCAGGTTGGTCTTGCTGTGGTCCATg                            >  6:238970/1‑142 (MQ=255)                                                                                                                                gATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATCACCACCAGCAGGTTGGTCTTGCTGTGGTCCATGCCGaa                       >  7:167357/1‑147 (MQ=255)                                                                                                                                gATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATCACCACCAGCAGGTTGGTCTTGCTGTGGTCCATGCCGAAc                      >  6:150869/1‑148 (MQ=255)                                                                                                                                gATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATCACCACCAGCAGGTTGGTCTTGCTGTGGTCCATGCCGAAc                      <  5:150869/148‑1 (MQ=255)                                                                                                                                  tCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATCACCACCAGCAGGTTGGTCTTGCTGTGGTCCATGCCGAACAg                    >  3:361729/1‑148 (MQ=255)                                                                                                                                    aaCAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATCACCACCAGCAGGTTGGTCTTGCTGTGGTCCATGCCGAACAGGt                  <  1:223738/148‑1 (MQ=255)                                                                                                                                     aCAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATCACCACCAGCAGGTTGGTCTTGCTGTGGTCCATGCCGAACAGGTAt                >  1:205385/1‑149 (MQ=255)                                                                                                                                       aGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATCACCACCAGCAGGTTGGTCTTGCTGTGGTCCATGCCGAACAGGTATTc              <  4:806/149‑1 (MQ=255)                                                                                                                                         gCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATCACCACCAGCAGGTTGGTCTTGCTGTGGTCCATGCCGAACAGGTATTCCa            <  1:40003/149‑1 (MQ=255)                                                                                                                                             cTGAACATCATGCTGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATCACCACCAGCAGGTTGGTCTTGCTGTGGTCCATGCCGAACAGGTATTCCAGGcc        >  3:115681/1‑149 (MQ=255)                                                                                                                                               gAACATCATGCTGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATCACCACCAGCAGGTTGGTCTTGCTGTGGTCCATGCCGAACAGGTATTCCAGGCCg       >  6:320841/1‑148 (MQ=255)                                                                                                                                                 aCAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATCACCACCAGCAGGTTGGTCTTGCTGTGGTCCATGCCGAACAGGTATTCCAGGCCGGa     <  6:64153/148‑1 (MQ=255)                                                                                                                                                   aGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATCACCACCAGCAGGTTGGTCTTGCTGTGGTCCATGCCGAACAGGTATTCCAGGCCGGACGa  >  5:179801/1‑149 (MQ=255)                                                                                                                                                   aGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATCACCACCAGCAGGTTGGTCTTGCTGTGGTCCATGCCGAACAGGTATTCCAGGCCGGACGa  <  3:327746/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    ACTTGCCGGCTTCGCCTTCATACACATAGAGGTCGAAGGTTTTCAGCACGATGCCGTTCAGGTAGTCGCCAACGTTCTGCACGAAGCCGTTGAGCAGGTGCAGGGTTTCGCCGCCCAGCAGCACGAAGATCAACAGGCCACTGAACAGCATGATGTTCAGGTTGGACAGGCGGCGGATGCCGTTCTCCACGCCCGATACCGCTGCCACGGTAGCCACGCCGGCCATGACCAGGATCACCACCAGCAGGTTGGTCTTGCTGTGGTCCATGCCGAACAGGTATTCCAGGCCGGACGA  >  NC_002947/5772005‑5772299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑