| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 92,184 | G→T | 5.1% | L66M (CTG→ATG) | trpA ← | tryptophan synthase subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 92,184 | 0 | G | T | 5.1% | 319.5 / 5.3 | 97 | L66M (CTG→ATG) | trpA | tryptophan synthase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base G (49/43); new base T (2/3); total (51/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.15e-01 | |||||||||||
GTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGACCAGTGCCGAG > NC_002947/92042‑92332 | gtgtCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGAt > 7:143505/1‑149 (MQ=255) cACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCATCCAGCTGGCCa < 2:136280/149‑1 (MQ=255) cACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGAACGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCa > 1:89800/1‑149 (MQ=255) cGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCATCCAGCTGGCCAcc < 6:137608/149‑2 (MQ=255) gCCAAAGCGGTGGATAGGGTTGTAGTAGACCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACgc > 6:348909/1‑149 (MQ=255) ccAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACgcc < 3:90481/149‑1 (MQ=255) ggTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTc < 2:212982/148‑1 (MQ=255) ggTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTc > 1:212982/1‑148 (MQ=255) ggTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCg < 2:330815/149‑1 (MQ=255) ggTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCg > 3:354472/1‑149 (MQ=255) gTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTAGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCg‑g > 8:19099/1‑149 (MQ=255) gtagtaGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTg < 2:320184/148‑1 (MQ=255) tagtagCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACCTCTTAATATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCg‑g < 1:364536/148‑1 (MQ=255) gCCCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGc < 1:321591/148‑1 (MQ=255) gCCCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGc < 5:121547/148‑1 (MQ=255) cccATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACgcc < 3:326509/122‑1 (MQ=255) cccATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACgcc > 4:326509/1‑122 (MQ=255) cccATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGGCATCGGGTCG‑GTGAACGGCAt > 4:293802/1‑149 (MQ=255) ccATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATg > 1:259222/1‑149 (MQ=255) aTCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCTCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCTCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAAc < 8:219863/141‑1 (MQ=255) aTCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAAc > 7:219863/1‑141 (MQ=255) aTCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGcc < 8:412580/149‑1 (MQ=255) aTCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGcc > 1:215968/1‑149 (MQ=255) gCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGc < 8:143505/149‑1 (MQ=255) cACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGCCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGACG‑GTGAACGGCATGCCCAGCt > 4:37591/1‑149 (MQ=255) cGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCg < 3:282517/149‑1 (MQ=255) aTCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGAt < 2:89800/149‑1 (MQ=255) ggCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCATCCAGCTGGCCAccct > 8:66070/1‑104 (MQ=255) ggCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACgccatcggccatc < 1:158255/117‑1 (MQ=255) ggCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACgccatcggccatc > 2:158255/1‑117 (MQ=255) ggCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGCTGGCCATCCAGCTGGCCAccct < 7:66070/107‑4 (MQ=255) gCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACg > 8:311003/1‑149 (MQ=255) cGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGc > 6:421216/1‑130 (MQ=255) cGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGc < 5:421216/130‑1 (MQ=255) gTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTc < 2:76547/149‑1 (MQ=255) tGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCg < 8:248922/149‑1 (MQ=255) tGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCg > 7:49919/1‑149 (MQ=255) ggTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCgg > 3:79830/1‑149 (MQ=255) gTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCgg < 4:79830/148‑1 (MQ=255) tCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGgcg > 6:264683/1‑149 (MQ=255) cTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGgcgc > 6:286793/1‑149 (MQ=255) gTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGgcgc < 4:126404/146‑1 (MQ=255) gTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCcag > 7:278291/1‑149 (MQ=255) ca‑caCGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGACAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCg > 4:150849/1‑149 (MQ=255) a‑cacGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCAc < 3:70460/131‑1 (MQ=255) a‑cacGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCAc > 4:70460/1‑131 (MQ=255) gA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGc < 5:348909/149‑1 (MQ=255) ttCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCt > 1:402732/1‑149 (MQ=255) gcgAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCGACGCCATCGGGCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCGttta < 6:2075/149‑3 (MQ=255) gcgAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCagcagc < 4:173040/135‑1 (MQ=255) gcgAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCagcagc > 3:173040/1‑135 (MQ=255) aCCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGAt < 7:311003/149‑1 (MQ=255) tGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGCTCTGcca > 6:301054/1‑147 (MQ=255) cAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGc < 3:331549/145‑1 (MQ=255) cAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCaa < 8:49919/147‑1 (MQ=255) gtgTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCAACGGCCATCGGGGCG‑GTGCACGGCATGCCCAGCGCGAACACGACGGCGCCCGCAGCCGCCAGGCCGGGGAGGCACTGCAACgcg > 8:272514/3‑147 (MQ=255) gggTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCCCGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAg > 7:102742/1‑149 (MQ=255) gggTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGccc > 7:188833/1‑131 (MQ=255) gggTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGccc < 8:188833/131‑1 (MQ=255) gggTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAg < 2:259222/149‑1 (MQ=255) ggTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAgg > 4:130335/1‑149 (MQ=255) tCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGcg > 8:352904/1‑149 (MQ=255) ttGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGcgtc > 5:360180/1‑149 (MQ=255) gACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGcgtc < 6:265856/147‑1 (MQ=255) cAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGc > 7:25263/1‑149 (MQ=255) cAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGc > 1:100961/1‑149 (MQ=255) cAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGc < 5:301054/149‑1 (MQ=255) gTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCgg > 3:117497/1‑149 (MQ=255) tCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGCGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCgg > 1:131890/1‑149 (MQ=255) tGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTc > 1:382171/1‑149 (MQ=255) tGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTc > 3:247407/1‑149 (MQ=255) gtcAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTc < 3:150849/145‑1 (MQ=255) gCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGa > 1:295171/1‑129 (MQ=255) gCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGa < 2:295171/129‑1 (MQ=255) gCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTc < 6:360180/147‑1 (MQ=255) gCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGc > 8:257929/1‑149 (MQ=255) cAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCGCGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCa < 4:358244/149‑1 (MQ=255) cTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCagcagc > 1:379142/1‑97 (MQ=255) cTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCagcagc < 2:379142/97‑1 (MQ=255) ttCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGTCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCtg > 8:29497/1‑149 (MQ=255) tCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCtgt > 4:297225/1‑149 (MQ=255) cAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGTTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGcgtcgt < 3:18418/129‑1 (MQ=255) cAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGcgtcgt > 4:18418/1‑129 (MQ=255) cAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCtgt < 5:286793/148‑1 (MQ=255) aaGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGa > 1:106004/1‑149 (MQ=255) gggCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGAc > 4:308487/1‑148 (MQ=255) gcgcgcAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGaa < 2:100961/149‑1 (MQ=255) cgcgcAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAg < 4:354472/149‑1 (MQ=255) cgcgcAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAg < 2:402732/149‑1 (MQ=255) gatggcgTGGCCATCCAGCTGGCCACCCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGt > 1:101107/7‑149 (MQ=255) aGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAATGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGAc > 5:276621/1‑149 (MQ=255) ggTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGACCa < 5:176375/149‑1 (MQ=255) gCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAATGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGACCAGTGc < 6:276621/149‑1 (MQ=255) gCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGACCAGTGc > 4:131507/1‑149 (MQ=255) ccAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGACCAGTGcc > 1:128563/1‑149 (MQ=255) ccAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGAGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGACCAGTgat > 7:94952/1‑147 (MQ=255) aGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGACCAGTGCCGa < 2:215968/149‑1 (MQ=255) gCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGACCAGTGCCGAg > 7:326472/1‑149 (MQ=255) | GTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGACCAGTGCCGAG > NC_002947/92042‑92332 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |