Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,785,965	C→A	5.6%	F323L (TTC→TTA) ‡	PP_5068 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,785,965	0	C	A	5.6%	288.7 / 4.9	89	F323L (TTC→TTA) ‡	PP_5068	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (43/41);  new base A (2/3);  total (45/44)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

CCAACTTCATCTGCAACCACTCCGACGACCGTGGCCGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACATGGTGCTGGTCGAGCGCCTGCTGCAGTGCATGCAACGCGGTGGCGTGGACTACAGCCTGTTCTTCCGC  >  NC_002947/5785817‑5786109                                                                                                                                                      |                                                                                                                                                 ccAACTTCATCTGCAACCACTCCGACGACCGTGGCCGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTc                                                                                                                                                  >  2:349239/1‑149 (MQ=255)  cAACTTCATCTGCAACCACTCCGACGACCGTGGCCGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTcc                                                                                                                                                 >  3:338954/1‑149 (MQ=255)  cAACTTCATCTGCAACCACTCCGACGACCGTGGCCGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTcc                                                                                                                                                 >  8:319031/1‑149 (MQ=255)  cAACTTCATCTGCAACCACTCCGACGACCGTGGCCGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTcc                                                                                                                                                 <  6:377116/149‑1 (MQ=255)   aaCTTCATCTGCAACCACTCCGACGACCGTGGCCGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCt                                                                                                                                                <  2:53870/149‑1 (MQ=255)      ttCATCTGCAACCACTCCGACGACCGTGGCCGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGcc                                                                                                                                             >  1:28718/1‑149 (MQ=255)       tCATCTGCAACCACTCCGACGACCGTGGACGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCg                                                                                                                                            >  3:97516/1‑149 (MQ=255)         aTCTGCAACCACTCCGACGACCGTGGCCGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCt                                                                                                                                          >  6:63451/1‑149 (MQ=255)          tCTGCAACCACTCCGACGACCGTGGCCGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTg                                                                                                                                         >  4:204202/1‑149 (MQ=255)            tGCAACCACTCCGACGACCGTGGCCGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTa                                                                                                                                       >  4:146208/1‑149 (MQ=255)                  cagtCCGACGACGGTGGCCGCTTCATCTACGCCAACCAGGTGCCCTTCGCCCAGTTGAACCTCAGCGCGGTGGGCCAGGC‑ACTGACCACCGTCTTCGAAGTTGAGCCTGTGAAGGATGCTTTGGGCCTGTTCCTGCCGCTGTACCAGGc                                                                                                                                 <  2:370264/146‑1 (MQ=255)                   aCTCCGACGACCGTGGCCGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGcc                                                                                                                                >  3:266482/1‑149 (MQ=255)                   aCTCCGACGACCGTGGCCGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGcc                                                                                                                                >  7:255573/1‑149 (MQ=255)                   aCTCCGACGACCGTGGCCGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGGTACAGCGGCAGGAACAGGcc                                                                                                                                <  6:113210/149‑1 (MQ=255)                    cTCCGACGACCGTGGCCGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGccc                                                                                                                               <  4:11103/149‑1 (MQ=255)                    cTCCGACGACCGTGGACGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCACAGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGccc                                                                                                                               <  5:31777/149‑1 (MQ=255)                     tCCGACGACCGTGGCCGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCa                                                                                                                              >  6:394393/1‑149 (MQ=255)                     tCCGACGACCGTGGCCGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGGTACAGCGGCAGGAACAGGccc                                                                                                                               >  2:281260/1‑148 (MQ=255)                       cgacgaCCGTGGCCGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCAtt                                                                                                                            <  1:271894/149‑1 (MQ=255)                       cgacgaCCGTGGCCGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCAt                                                                                                                             >  1:251074/1‑148 (MQ=255)                       cgacgaCCGTGGCCGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCAt                                                                                                                             >  6:101018/1‑148 (MQ=255)                       cgacgaCCGTGGCCGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGGTACAGCGGCAGGAACAGGccc                                                                                                                               <  1:281260/146‑1 (MQ=255)                       cgacgaCCGTGGCCGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTGGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCAt                                                                                                                             >  7:347857/1‑148 (MQ=255)                           gacCGTGGCCGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCt                                                                                                                        >  5:46889/1‑149 (MQ=255)                           gacCGTGGCCGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCt                                                                                                                        <  7:146504/149‑1 (MQ=255)                               gTGGCCGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGAc                                                                                                                    <  2:392543/149‑1 (MQ=255)                                tGGCCGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGAcc                                                                                                                   >  6:150420/1‑149 (MQ=255)                                  gCCGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCt                                                                                                                  >  6:17427/1‑148 (MQ=255)                                  gCCGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCt                                                                                                                  >  8:398798/1‑148 (MQ=255)                                    cGCTACAGCTATGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGAt                                                                                                               >  7:228988/1‑149 (MQ=255)                                    cGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGAt                                                                                                               <  5:17427/149‑1 (MQ=255)                                    cGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGAt                                                                                                               >  1:283839/1‑149 (MQ=255)                                     gCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATg                                                                                                              <  5:101018/149‑1 (MQ=255)                                         cAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATgcg                                                                                                            <  7:87480/147‑1 (MQ=255)                                          aGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCg                                                                                                         <  2:283839/149‑1 (MQ=255)                                           gCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGCGGATGACAACCGTGATCGAAGTTGCGCCACTGAAGGAACCGTTGGGCCTGTTCCTGCCGCTGTA‑CAGGCCCATTACCTGGCCCTGATGCGCCgg                                                                                                        >  1:337521/1‑149 (MQ=255)                                               cGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGgcgc                                                                                                     <  4:18623/148‑1 (MQ=255)                                               cGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGgcg                                                                                                      <  2:294125/147‑1 (MQ=255)                                                  cAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAg                                                                                                 <  8:327015/149‑1 (MQ=255)                                                   aaCCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAgg                                                                                                <  3:204202/149‑1 (MQ=255)                                                   aaCCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAgg                                                                                                >  7:110875/1‑149 (MQ=255)                                                    aCCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAggg                                                                                               <  8:347857/149‑1 (MQ=255)                                                         gggCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCTAAGTTGAGCCACTGAAGTAAGCGTTGTTCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGac                                                                                          <  1:349239/147‑1 (MQ=255)                                                         gTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGac                                                                                          <  7:319031/149‑1 (MQ=255)                                                            cccATCGCCAACTGGAACCTCAGCGAGCTGGACCAGGC‑ACTGACAACCGTGATCGAAGTTGATCCACTGAAGGAAGCGTTGGGCATGTTCCTGCCGCTGTACCAAGCCCATTACATGGACATGATGCGCCGGCGCCTATGGCTGaccaa                                                                                       >  8:13361/1‑148 (MQ=255)                                                              cATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCTAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCAccg                                                                                     <  6:362527/149‑1 (MQ=255)                                                               aTCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCAccgc                                                                                    <  8:110875/149‑1 (MQ=255)                                                                  gCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCAccgccg                                                                                  <  2:251074/148‑1 (MQ=255)                                                                   cccACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGaa                                                                                <  4:338954/149‑1 (MQ=255)                                                                   cccACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTGGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCAccgccg                                                                                  >  5:415730/1‑147 (MQ=255)                                                                    ccACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCAccgccg                                                                                  >  4:289388/1‑146 (MQ=255)                                                                    ccACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAg                                                                               <  7:398798/149‑1 (MQ=255)                                                                      aCTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAgat                                                                             <  4:266482/149‑1 (MQ=255)                                                                       cTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAgatg                                                                            <  5:150420/149‑1 (MQ=255)                                                                       cTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAgat                                                                             >  8:114401/1‑148 (MQ=255)                                                                          gAACCGCAGCGCGCTGGCCCAGGC‑ACGTACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACTTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGatgatg                                                                         <  8:374010/149‑1 (MQ=255)                                                                           aaCCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGAtgatga                                                                        <  8:228988/149‑1 (MQ=255)                                                                              cTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACAt                                                                     >  3:357606/1‑149 (MQ=255)                                                                              cTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACAt                                                                     <  3:146208/149‑1 (MQ=255)                                                                              cTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACAAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGGAGATGATGACAt                                                                     >  2:348942/1‑149 (MQ=255)                                                                               tCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACAt                                                                     >  4:2988/1‑148 (MQ=255)                                                                                 aGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACAt                                                                     <  3:2988/146‑1 (MQ=255)                                                                                          gCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACATGGTGCTGGTc                                                           >  6:217048/1‑147 (MQ=255)                                                                                          gCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACATGGTGCTGGTc                                                           >  7:372184/1‑147 (MQ=255)                                                                                           cccAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACATGGTGCTGGTCGAg                                                        <  8:372184/149‑1 (MQ=255)                                                                                             cAGGC‑ACTGACCACCGTGATCGAAGTAGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCACATTACCTGGACCTGATGCGGCGGCGCCTAGGGCTGcccacc                                                                                      >  7:151826/1‑117 (MQ=255)                                                                                                   aCTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACATGGTGCTGGTCGAGCGCctgc                                                 <  2:28718/149‑1 (MQ=255)                                                                                                   aCTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACATGGTGCTGGTCGAGCGCctgc                                                 <  3:289388/149‑1 (MQ=255)                                                                                                         caccGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACATGGTGCTGGTCGAGCGCCTGCTGCAGt                                           >  3:10807/1‑149 (MQ=255)                                                                                                         caccGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACATGGTGCTGGTCGAGCGCCTGCTGCAGt                                           <  2:359182/149‑1 (MQ=255)                                                                                                          accGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACATGGTGCTGGTCGAGCGCCTGCTGCAGtg                                          >  3:427536/1‑149 (MQ=255)                                                                                                            cGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACATGGTGCTg                                                              >  8:263263/1‑127 (MQ=255)                                                                                                            cGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACATGGTGCTg                                                              <  7:263263/127‑1 (MQ=255)                                                                                                              tGATCGAAGTTGAGCCAATGAAGGAAGCGTTGGGCATGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCAGAGGGCTGACCACCGCCGAAGATGATGACATGGTGATGGTCGATCGCATGCTGCATtgcagg                                      >  8:271929/1‑147 (MQ=255)                                                                                                                   gAAGTTGAGCGACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGaa                                                                                <  1:73363/102‑1 (MQ=255)                                                                                                                   gAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGaa                                                                                >  2:73363/1‑102 (MQ=255)                                                                                                                   gAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACATGGTGCTGGTCGAGCGCCTGCTGCAGTGCATGCAAcg                                 >  4:287473/1‑149 (MQ=255)                                                                                                                       ttGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACATGGTGCTGGTCGAGCGCCTGCTGCAGTGCATGCAACGCGGt                             <  5:394393/149‑1 (MQ=255)                                                                                                                             cACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACATGGTGCTGGTCGAGCGCCTGCTGCAGTGCATGCAACGCGGTGGCGTg                       <  6:415730/149‑1 (MQ=255)                                                                                                                                     gaagCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACATGGTGCTGGTCGAGCGCCTGCTGCAGTGCATGCAACGCGGTGGCGTGGACTACAg               >  3:142491/1‑149 (MQ=255)                                                                                                                                       agCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATAACCAGGACCTGATGCGCCGGCGACTAGGGCTGACCACCGCCGATGATGATGACATGGTGCTGGTCGACAGCCTGCTGCAGTGCATGCAACGCGGTGGCGTGGACTACAGcc             >  7:396861/1‑149 (MQ=255)                                                                                                                                         taatGGGCCTGGTACAGCGGCAGGAACAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACATGGTGCTGGTCGAGCGCCTGCTGCa                                             <  7:197986/112‑1 (MQ=37)                                                                                                                                         taatGGGCCTGGTACAGCGGCAGGAACAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACATGGTGCTGGTCGAGCGCCTGCTGCa                                             >  8:197986/4‑115 (MQ=37)                                                                                                                                          gTTTGGCCTGTTCCTGCCGCTTTACCATGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTTACCACCGCCGAAGATGATGACATTGTGCTGGTCGAGCGCCTGCTGCAGTGCATGCAACGCGGTGGCGTGGACTACAGCCTg           <  4:97516/148‑1 (MQ=255)                                                                                                                                              ggCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACATGGTGCTGGTCGAGCGCCTGCTGCAGTGCATGCAACGCGGTGGCGTGGACTACAGCCTg           >  4:119675/1‑144 (MQ=255)                                                                                                                                              ggCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACATGGTGCTGGTCGAGCGCCTGCTGCAGTGCATGCAACGCGGTGGCGTGGACTACAGCCTg           <  3:119675/144‑1 (MQ=255)                                                                                                                                               gCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACATGGTGCTGGTCGAGCGCCTGCTGCAGTGCATGCAACGCGGTGGCGTGGACTACAGCCTGtgcttc     >  8:183515/1‑149 (MQ=255)                                                                                                                                               cccTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCACACCACCGCCGAAGATGATGACATGGTGCTGGTCGAGCGCCTCCTGCAGTGCATGCAACGCGGTCGCGTCGACTACAGCATcttctta     >  2:86947/2‑148 (MQ=255)                                                                                                                                                ccTGTTCCTGCCTCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACATGGTGCTGGTCGAGCGCCTGCTGCAGTGCATGCAACGCGGTGGCGTGGACTACAGCCTGTTCTTcc    <  7:13361/149‑1 (MQ=255)                                                                                                                                                ccTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACATGGTGCTGGTCGAGCGCCTGCTGCAGTGCATGCAACGCGGTGGCGTGGACTACAGCCTGTTCTTcc    <  3:119214/149‑1 (MQ=255)                                                                                                                                                ccTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACATGGTGCTGGTCGAGCGCCTGCTGCAGTGCATGCAACGCGGTGGCGTGGACTACAGCCTGTTCTTcc    >  3:106793/1‑149 (MQ=255)                                                                                                                                                 cTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACATGGTGCTGGTCGAGCGCCTGCTGCAGTGCATGCAACGCGGTGGCGTGGACTACAGCCTGTTCTTCCg   >  8:334050/1‑149 (MQ=255)                                                                                                                                                   gTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACATGGTGCTGGTCGAGCGCCTGCTGCAGTGCATGCAACGCGGTGGCGTGGACTACAGCCTGTTCTTCCGc  >  1:264059/1‑148 (MQ=255)                                                                                                                                                      |                                                                                                                                                 CCAACTTCATCTGCAACCACTCCGACGACCGTGGCCGCTACAGCTACGCCAACCAGGTGCCCATCGCCCACTGGAACCTCAGCGCGCTGGCCCAGGC‑ACTGACCACCGTGATCGAAGTTGAGCCACTGAAGGAAGCGTTGGGCCTGTTCCTGCCGCTGTACCAGGCCCATTACCTGGACCTGATGCGCCGGCGCCTAGGGCTGACCACCGCCGAAGATGATGACATGGTGCTGGTCGAGCGCCTGCTGCAGTGCATGCAACGCGGTGGCGTGGACTACAGCCTGTTCTTCCGC  >  NC_002947/5785817‑5786109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑