BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,786,988	C→A	5.4%	S134* (TCG→TAG) ‡	trxC →	thioredoxin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,786,988	0	C	A	5.4%	326.7 / 5.3	103	S134* (TCG→TAG) ‡	trxC	thioredoxin
Reads supporting (aligned to +/- strand): ref base C (51/46); new base A (3/3); total (54/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

Only 100 of 103 total aligned reads displayed.

TTGCTGGCCGCTGCCGCCTGGCCAAGCTCGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCGTGGTGCGATTCGCGCATTTTCAC  >  NC_002947/5786841‑5787132

ttGCTGGCCGCTGCCGCCTGGCCAAGCTCGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCg                                                                                                                                                 >  3:345038/1‑149 (MQ=255)

ttGCTGGCCGCTGCCGCCTGGCCAAGCTCGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCg                                                                                                                                                 >  7:119582/1‑149 (MQ=255)

ttGCTGGCCGCTGCCGCCTGGCCAAGCTCGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCg                                                                                                                                                 >  2:19080/1‑149 (MQ=255)

    tGGCCGCTGCCGCCTGGCCAAGCTCGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCgctgc                                                                                                                                             >  7:182522/1‑149 (MQ=255)

      gCCGCTGCCGCCTGGCCAAGCTCGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGctgctg                                                                                                                                           <  7:399795/149‑1 (MQ=255)

         gCTGCCGCCTGGCCAAGCTCGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCTAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAg                                                                                                                                        <  7:90267/149‑1 (MQ=255)

             ccgccTGGCCAAGCTCGACAGCGAAGCCAACCGTACCCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGCAGCGACTGGAGTgga                                                                                                                                    <  5:287137/149‑2 (MQ=255)

              cgccTGGCCAAGCTCGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGTGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCt                                                                                                                                   <  2:302088/149‑1 (MQ=255)

               gccTGGCCAAGCTCGACAGTGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTg                                                                                                                                  >  7:203933/1‑149 (MQ=255)

               gccTGGCCAAGCTCGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGCAGCGACTGGAGt                                                                                                                                       <  1:46682/144‑1 (MQ=255)

               gccTGGCCAAGCTCGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGCAGCGACTGGAGt                                                                                                                                       >  2:46682/1‑144 (MQ=255)

                 cTGGCCAAGCTCGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCg                                                                                                                                >  2:232511/1‑149 (MQ=255)

                      cAAGCTCGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGcc                                                                                                                           <  4:345038/149‑1 (MQ=255)

                      cAAGCTCGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGcc                                                                                                                           <  6:96820/149‑1 (MQ=255)

                         gCTCGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAg                                                                                                                        <  1:232511/149‑1 (MQ=255)

                          cTCGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAgg                                                                                                                       >  3:52518/1‑149 (MQ=255)

                          cTCGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAgg                                                                                                                       <  3:10776/149‑1 (MQ=255)

                            cGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAgggg                                                                                                                     >  7:176642/1‑149 (MQ=255)

                             gACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCATGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGt                                                                                                                    <  3:64661/149‑1 (MQ=255)

                             gACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGc                                                                                                                                 <  5:259297/136‑1 (MQ=255)

                             gACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGc                                                                                                                                 >  6:259297/1‑136 (MQ=255)

                             gACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAggg                                                                                                                      <  2:421028/147‑1 (MQ=255)

                             gACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGt                                                                                                                    >  6:234089/1‑149 (MQ=255)

                             gACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCACGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGt                                                                                                                    >  1:310261/1‑149 (MQ=255)

                               cAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGtt                                                                                                                   >  6:247654/1‑148 (MQ=255)

                                aGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGttt                                                                                                                  >  1:425653/1‑148 (MQ=255)

                                      gCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGtt                                                                                                           >  8:84321/1‑149 (MQ=255)

                                      gCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCACCCGCCACGCCGGGGCCTTTCCACTCCACTCGCTGCTCGACTGGCTCCGTAGCAAAGGCGTTTAAGAGtg                                                                                                           >  4:348661/1‑148 (MQ=255)

                                      gCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCCGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGtt                                                                                                           >  7:35927/1‑149 (MQ=255)

                                       ccAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGttt                                                                                                          >  2:280668/1‑149 (MQ=255)

                                       ccAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGCAGCGACTGGAGTgg                                                                                                                                     >  5:310971/1‑122 (MQ=255)

                                       ccAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGCAGCGACTGGAGTgg                                                                                                                                     <  6:310971/122‑1 (MQ=255)

                                         aaCCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTttc                                                                                                        >  3:140501/1‑149 (MQ=255)

                                           ccGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTttctt                                                                                                      >  8:75302/1‑149 (MQ=255)

                                            cGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTttcttc                                                                                                     <  7:75302/149‑1 (MQ=255)

                                            cGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTttctt                                                                                                      >  6:135171/1‑148 (MQ=255)

                                             gTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCa                                                                                                    <  2:310261/149‑1 (MQ=255)

                                              tAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCaa                                                                                                   <  2:251070/149‑1 (MQ=255)

                                              tAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCaa                                                                                                   <  2:289668/149‑1 (MQ=255)

                                                aCCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAag                                                                                                  <  4:140501/148‑1 (MQ=255)

                                                 ccTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAag                                                                                                  >  8:251209/1‑147 (MQ=255)

                                                       aGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAAt                                                                                          >  6:197658/1‑149 (MQ=255)

                                                       aGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAAt                                                                                          <  5:234089/149‑1 (MQ=255)

                                                        gggCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATg                                                                                         <  5:212451/149‑1 (MQ=255)

                                                        gggCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTAAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATg                                                                                         <  4:67318/149‑1 (MQ=255)

                                                         ggCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATg                                                                                         >  7:247381/1‑148 (MQ=255)

                                                           cAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAg                                                                                      <  7:77628/149‑1 (MQ=255)

                                                             aCTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCGGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCt                                                                                    >  5:432286/1‑149 (MQ=255)

                                                              cTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTc                                                                                   <  8:385386/149‑1 (MQ=255)

                                                                 ggCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGa                                                                                 >  5:434593/1‑148 (MQ=255)

                                                                 ggCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGAc                                                                                <  8:203933/149‑1 (MQ=255)

                                                                 ggCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGAc                                                                                <  6:434593/149‑1 (MQ=255)

                                                                 ggCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGAc                                                                                <  7:39030/149‑1 (MQ=255)

                                                                    aTACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACaaa                                                                             >  8:109497/1‑149 (MQ=255)

                                                                     tACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAAc                                                                            >  2:224372/1‑149 (MQ=255)

                                                                     tACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAAc                                                                            >  4:395933/1‑149 (MQ=255)

                                                                       cGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAAct                                                                           <  7:251209/148‑1 (MQ=255)

                                                                        gCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAActgc                                                                         >  8:212567/1‑149 (MQ=255)

                                                                        gCTCGATCACCAGCCTGCTGCTGTTCAAGAACGGACGAGAAATCACCCGACAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAActgc                                                                         >  1:11402/1‑149 (MQ=255)

                                                                         cTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAActgct                                                                        >  1:68182/1‑149 (MQ=255)

                                                                          tCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCTGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAActgct                                                                        >  2:199488/1‑148 (MQ=255)

                                                                            gATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGa                                                                      <  4:52518/148‑1 (MQ=255)

                                                                            gATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGa                                                                      <  8:26619/148‑1 (MQ=255)

                                                                               ccccAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGt                                                                    >  5:363653/1‑147 (MQ=255)

                                                                               ccccAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGt                                                                    >  1:25808/1‑147 (MQ=255)

                                                                                cccAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGt                                                                                                                                       >  8:77024/1‑79 (MQ=255)

                                                                                cccAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGt                                                                                                                                       <  7:77024/79‑1 (MQ=255)

                                                                                 ccAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGc                                                                <  8:176642/149‑1 (MQ=255)

                                                                                 ccAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGc                                                                >  2:421136/1‑149 (MQ=255)

                                                                                     ccTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTtgt                                                            <  8:113931/149‑1 (MQ=255)

                                                                                                tCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAActg                                                                          >  7:93807/1‑124 (MQ=255)

                                                                                                  aaGAACGGCCGCGAAATCAGCCGCCGGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAg                                               <  6:432286/149‑1 (MQ=255)

                                                                                                  aaGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAg                                               <  1:224372/149‑1 (MQ=255)

                                                                                                  aaGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAg                                               >  3:367608/1‑149 (MQ=255)

                                                                                                     aaCGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAg                                                                 >  8:55091/1‑128 (MQ=255)

                                                                                                     aaCGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAg                                                                 <  7:55091/128‑1 (MQ=255)

                                                                                                       cGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGcc                                                         <  6:164133/134‑1 (MQ=255)

                                                                                                       cGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGcc                                                         >  5:164133/1‑134 (MQ=255)

                                                                                                       cGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGaa                                          <  5:135171/149‑1 (MQ=255)

                                                                                                          ccGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAAt                                         >  6:13750/1‑147 (MQ=255)

                                                                                                           cgcgAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAg                                      >  1:390262/1‑149 (MQ=255)

                                                                                                              gAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCgg                                   >  3:35407/1‑149 (MQ=255)

                                                                                                               aaaTCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGc                                  <  3:395933/149‑1 (MQ=255)

                                                                                                                aaTCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCgg                                   >  7:101195/1‑147 (MQ=255)

                                                                                                                  tCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCgg                                   >  6:358432/1‑145 (MQ=255)

                                                                                                                  tCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCgg                                   <  5:358432/145‑1 (MQ=255)

                                                                                                                       cgccAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGc                          <  8:35927/149‑1 (MQ=255)

                                                                                                                          cAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCgtg                       <  1:280668/149‑1 (MQ=255)

                                                                                                                             gCCGGGGCGTTTCCACTCCAGTCTCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCgtggtg                    <  8:119582/149‑1 (MQ=255)

                                                                                                                             gCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCgtggtg                    <  2:390262/149‑1 (MQ=255)

                                                                                                                              ctggtgCGTTTCCACTCCAGTCGCTGCTGTATTGGCTGCTTATCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCgtggtg                    <  5:122723/143‑1 (MQ=255)

                                                                                                                                  ggCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCGTGGTGCGAtt               <  7:109497/149‑1 (MQ=255)

                                                                                                                                     gTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCGTGGTGCGATTcgc            <  8:247381/149‑1 (MQ=255)

                                                                                                                                      tttCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCGTGGTGCGATTcgcg           >  2:280030/1‑149 (MQ=255)

                                                                                                                                        gccACTCCAGCAGCGACTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCGTGGTGCGATTCGCGCa         >  5:248139/2‑149 (MQ=255)

                                                                                                                                             tCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCGTGGTGCGATTCGCGCAtttt     >  7:238450/1‑148 (MQ=255)

                                                                                                                                             tCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCGTGGTGCGATTCGCGCAtttt     >  7:20658/1‑148 (MQ=255)

                                                                                                                                             tCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCGTGGTGCGATTCGCGCAtttt     >  7:14292/1‑148 (MQ=255)

                                                                                                                                             tCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCGTGGTGCGATTCGCGCAtttt     >  6:441852/1‑148 (MQ=255)

                                                                                                                                               cAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCGTGGTGCGATTCGCGCATTTTCAc  <  1:199488/149‑1 (MQ=255)

TTGCTGGCCGCTGCCGCCTGGCCAAGCTCGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCGTGGTGCGATTCGCGCATTTTCAC  >  NC_002947/5786841‑5787132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`