Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,821,897 T→A 7.0% L173Q (CTG→CAG)  metW → methionine biosynthesis protein MetW

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,821,8970TA 7.0% 245.6 / 5.6 85L173Q (CTG→CAG) metWmethionine biosynthesis protein MetW
Reads supporting (aligned to +/- strand):  ref base T (41/38);  new base A (3/3);  total (44/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.27e-01

CCGCTGGTACCTGGCGACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGACGCCCACCGGAGGAATCGCAC  >  NC_002947/5821751‑5822039
                                                                                                                                                  |                                                                                                                                              
ccGCTGGTACCTGGCGACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCt                                                                                                                                                <  3:235851/147‑1 (MQ=255)
  gCTGGTACCTGGCGACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCg                                                                                                                                            >  2:120595/1‑149 (MQ=255)
   cTGGTACCTGGCGACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCgg                                                                                                                                           <  3:378815/149‑1 (MQ=255)
      gTACCTGGCGACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCg                                                                                                                                        <  2:205367/149‑1 (MQ=255)
       tACCTGGCGACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCAGGCGGTCGa                                                                                                                                       <  1:409923/149‑1 (MQ=255)
        aCCTGGCGACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGAc                                                                                                                                      >  7:328801/1‑149 (MQ=255)
            ggCGACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACt                                                                                                                                  >  6:282658/1‑149 (MQ=255)
               gACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGc                                                                                                                               >  5:392935/1‑149 (MQ=255)
               gACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCCACCGCCTGGCGGTCGACCACTTGc                                                                                                                               >  6:305533/1‑149 (MQ=255)
                  cAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCAcc                                                                                                                            <  4:369552/149‑1 (MQ=255)
                  cAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCAcc                                                                                                                            >  5:356035/1‑149 (MQ=255)
                  cAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCAcc                                                                                                                            >  1:437594/1‑149 (MQ=255)
                  cAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCAGGCGGTCGagt                                                                                                                                     >  1:245953/1‑138 (MQ=255)
                  cAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCAGGCGGTCGagt                                                                                                                                     <  2:245953/140‑3 (MQ=255)
                   aaaGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCg                                                                                                                           >  6:39016/1‑149 (MQ=255)
                    acggCCGCATGCGGGTATCGTACTTCATGCCTTATACCTTGTACAACACGCCTAACATCCACTTCTGCACCTTCCCCGACTTCCAACAACTTGTGCACCAACGCGGGGCCATGGTACTCGACCGCCTGGGGGTCGACCACTTGCACCGc                                                                                                                          <  2:437594/147‑1 (MQ=255)
                        ccGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACg                                                                                                                      >  1:401603/1‑149 (MQ=255)
                                cGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTgg                                                                                                                                              >  5:172871/1‑117 (MQ=255)
                                cGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTgg                                                                                                                                              <  6:172871/117‑1 (MQ=255)
                                  gTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGggtggggtgg                                                                                                            >  6:11393/1‑149 (MQ=255)
                                     tCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCg                                                                                                         >  4:249470/1‑149 (MQ=255)
                                       ggACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGc                                                                                                       <  5:282658/149‑1 (MQ=255)
                                          cTTCATGCCGTATACCTGGTACAACACGCCGCACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTAt                                                                                                    <  5:11393/149‑1 (MQ=255)
                                           ttCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCAAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATg                                                                                                   >  4:101773/1‑149 (MQ=255)
                                             cATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGc                                                                                                 >  7:126041/1‑149 (MQ=255)
                                             cATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTACACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGc                                                                                                 >  7:386239/1‑149 (MQ=255)
                                              aTGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGcc                                                                                                >  8:362230/1‑149 (MQ=255)
                                                  cGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAAt                                                                                            >  1:297211/1‑149 (MQ=255)
                                                       aCCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAAtcttc                                                                                        >  7:320886/1‑148 (MQ=255)
                                                        ccTGGTACAACAGGCCGACCTTCCACTTCTGCACCTTCGCCGACTTCGATGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCGTGGCGGTCGACCACTTGCTCCGCAACGGGTGGGGTGGCCGGCTATGGCCTAAtcttct                                                                                       <  6:234094/148‑1 (MQ=255)
                                                        ccTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAAtcttc                                                                                        >  8:374289/1‑147 (MQ=255)
                                                        ccTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTa                                                                                      <  2:297211/149‑1 (MQ=255)
                                                           ggTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGt                                                                                   >  6:81409/1‑149 (MQ=255)
                                                            gTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGt                                                                                   <  5:81409/148‑1 (MQ=255)
                                                            gTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGGGGGGTGGCCGGCTATGGCCTAATCTTCTAGGt                                                                                   <  5:324010/148‑1 (MQ=255)
                                                               caacaCGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGt                                                                                   >  7:279648/1‑145 (MQ=255)
                                                                    cGCCTAACATCAACTTCTGCACCTTCGCCGACTTCGAATAACTTGTGAACTAACTCCGGTCCATGGTACTCTACCGTTTGGTGGTTGACCACTTGCACCGAAACGGGTGGGGTTGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGc                                                                          <  7:65750/149‑1 (MQ=255)
                                                                     gCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCa                                                                                                                                    >  3:291276/1‑90 (MQ=255)
                                                                      ccGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCAt                                                                        <  5:164064/149‑1 (MQ=255)
                                                                         aaCATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGt                                                                                   <  3:156031/135‑1 (MQ=255)
                                                                         aaCATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGt                                                                                   >  4:156031/1‑135 (MQ=255)
                                                                          aCATCCACTTCTGCACCTTCGCCGACTTCTAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTAc                                                                    <  6:92668/149‑1 (MQ=255)
                                                                           cATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTAcc                                                                   >  3:70087/1‑149 (MQ=255)
                                                                             tgcACTTCTGCAACTTCTCCTACTTCTAATAACTTGTGCAAGAAAGCCGGCGCAAGGTACTCCACGGCCTGGAGGTCGACCACTTGCACCGCTACGGGTGGTGTGGCCGGCTATGGCCTAATCTTCTATGTGAGATCGGCATCTACcgc                                                                 <  7:228190/147‑1 (MQ=255)
                                                                                aCTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGt                                                               >  4:325904/1‑148 (MQ=255)
                                                                                 cTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTga                                                                                 <  5:224592/129‑1 (MQ=255)
                                                                                 cTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTga                                                                                 >  6:224592/1‑129 (MQ=255)
                                                                                  ttCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTcag                                                            >  3:233498/1‑149 (MQ=255)
                                                                                        aCCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTcc                                                      >  2:416103/1‑149 (MQ=255)
                                                                                           ttCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCggg                                                   >  2:313208/1‑149 (MQ=255)
                                                                                             cGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGc                                                  <  3:325904/148‑1 (MQ=255)
                                                                                              gCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCt                                                >  3:12752/1‑149 (MQ=255)
                                                                                               ccGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTg                                               <  3:249470/149‑1 (MQ=255)
                                                                                                cGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGc                                              <  8:126041/149‑1 (MQ=255)
                                                                                                cGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGACTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTg                                               >  1:397870/1‑148 (MQ=255)
                                                                                                  aCTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGc                                              >  4:401668/1‑147 (MQ=255)
                                                                                                   cTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGc                                              >  5:266691/1‑146 (MQ=255)
                                                                                                    ttCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAaga                                          <  4:233498/149‑1 (MQ=255)
                                                                                                      cGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCTTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAagag                                         <  1:120595/148‑1 (MQ=255)
                                                                                                      cGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAaga                                          <  1:416103/147‑1 (MQ=255)
                                                                                                       gaagaaCTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTTGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGc                                        <  4:12752/148‑1 (MQ=255)
                                                                                                       gaagaaCTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCa                                       >  6:308476/1‑149 (MQ=255)
                                                                                                        aagaaCTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCAt                                      <  4:70087/149‑1 (MQ=255)
                                                                                                                gtgCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTcgc                              >  5:88450/1‑149 (MQ=255)
                                                                                                                gtgCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTcg                               <  8:279648/148‑1 (MQ=255)
                                                                                                                gtgCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTcg                               <  5:308476/148‑1 (MQ=255)
                                                                                                                 tgCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTcgcg                             <  2:77175/149‑1 (MQ=255)
                                                                                                                 tgCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTcgc                              <  1:313208/148‑1 (MQ=255)
                                                                                                                  gCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTcgcg                             <  7:362230/148‑1 (MQ=255)
                                                                                                                   cACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGt                           >  6:213971/1‑149 (MQ=255)
                                                                                                                   cACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGt                           <  6:266691/149‑1 (MQ=255)
                                                                                                                        aCGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGAc                      <  6:356035/149‑1 (MQ=255)
                                                                                                                            cGGGCCAAGGTACTCGACCGCCTGGCGGTCGCCCACTTGCACCGCAACGGGTGGGGTGACCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGACGccc                  >  2:415121/1‑149 (MQ=255)
                                                                                                                            cGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGACGccc                  <  5:39016/149‑1 (MQ=255)
                                                                                                                             gggCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGACGCCCa                 <  5:213971/149‑1 (MQ=255)
                                                                                                                               gCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGACGCCCAcc               >  5:216409/1‑149 (MQ=255)
                                                                                                                                ccAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGACGCCCACCg              <  2:401603/149‑1 (MQ=255)
                                                                                                                                   aGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGACGCCCACCggag           <  7:374289/149‑1 (MQ=255)
                                                                                                                                     gTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGACGCCCACCggagg          >  5:44584/1‑148 (MQ=255)
                                                                                                                                        gtCGACCGCCAGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGAc                      <  5:145197/132‑1 (MQ=255)
                                                                                                                                        gtCGACCGCCAGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGACGCCCACCGGAGGAATc      >  4:199813/2‑149 (MQ=255)
                                                                                                                                        gtCGACCGCCAGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTAGCGGTCTGAc                      >  6:145197/2‑133 (MQ=255)
                                                                                                                                         tCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGGGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTTCCCCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGACGCCAAGCGGAGGAATCg     >  6:280932/1‑149 (MQ=255)
                                                                                                                                           gACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGACGCCCACCGGAGGAATCGCa   <  7:377383/149‑1 (MQ=255)
                                                                                                                                            aCCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGACGCCCACCGGAGGAATCGCAc  >  6:67815/1‑149 (MQ=255)
                                                                                                                                            aCCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGACGCCCACCGGAGGAATCGCAc  <  3:101773/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
CCGCTGGTACCTGGCGACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGACGCCCACCGGAGGAATCGCAC  >  NC_002947/5821751‑5822039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑